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The map label for this gene is msmE [H]

Identifier: 238922898

GI number: 238922898

Start: 444806

End: 446107

Strand: Direct

Name: msmE [H]

Synonym: EUBREC_0486

Alternate gene names: 238922898

Gene position: 444806-446107 (Clockwise)

Preceding gene: 238922896

Following gene: 238922899

Centisome position: 12.89

GC content: 43.32

Gene sequence:

>1302_bases
ATGGCAAAAAGAAAGATTTGTCAGGCTATGGCGGTTGTGCTGGCACTCGGAATGTGCACGACAGGACTGACCGGATGTGG
AAATGAAAAGCGGGCGGACGGAAAAACTGAGATTGAGGTGGTTTCTTATAAGCCGGAGGCTGTTAAGGCTTTCGAGAAGA
TTGAGAAGCAATTTAATGAGACTCACGATGATATTCATCTGACTATCTCATCTCCGAATGAGGCTATGACTATTTTAAAG
ACACGCTTCATCCGTGAGGATTATCCGGATATCATTGCAATAGGTGGAGATATCAATTATTCAAATTTCCTGGATGCAGA
TTTGTTTGAGGATATCTCAGATCTGGATGCAGTGGACAATGTAAAAGAGGCATATCTGGATATGGACAAGGAGCTTGAGT
TCATTCCAAAGGATGGCACCTATGCACTTCCTTACGCCGCAAATGCAGCAGGAGTACTTTACAACAAGGATATGTTTGCG
GAGAATGGCTGGAAGGTGCCTACAACCTGGAGTGAGTTTACAGCACTGTGCGATGAGATAAAGGAAAGCGGAACACTTCC
TTTGTATCTGGGCTTTAAGGATACATGGACCTGTCTGGCACCGTGGAATGCTCTTGCAGTAGGTCTTTGTGATTCAGACA
CCTGTAATCAGGTAAATATGGGAAATACGACATTTGAGGAGGCATATTCTCCGGTTGCCGACAAGATACGTACATTGCTT
GATTATGCAGAGGACAATCCATATGCTTACAGTTACAATGACGCATGTACAGCCTTTGCCAGAGGTGAATCTGCCATGTA
CACGATAGGAAGCTATGCTATTCCTCAGATTAAGTCAGTTAATCCTGATATGAATATCGGTTCATTTACTTTCCCGGCAA
ATGATAATGAGGCTGACAATGTGTTAAATTCAGGAATAGATTTACAGCTTTCAGTTATGAAGGCCTGCAAAAATAAGGAA
GCAGCATACGAGGTGCTTGAATATCTGTACAGCGATGAGACAATTCAGACATATCTGGATGACCAGGGCGGAATTGCCTG
CAAGGAGGGAGATTTCGCAATACCTGATACACTGGAGGATATGCAGGAGTATATAAAGGACAACCGTATGTCGGATTATC
AGGATCACCATTATCCAAGTGAGATGAGCGTGGACGCCATGATTCAGACATATCTGCTTGACACAGGTGACAATGCAAAA
GAGAAGTTCTTAAAGAAATTTGATTCAGACTGGGAGCGTTACAACAGAGATCTGATTCGTGAAGTACAGGATTACCAGAA
GGAACAGGAGGATGCAAAATGA

Upstream 100 bases:

>100_bases
AAAAAAGCAAAAATAAGGGTATTTATTGTATGCATAATGGTATGTTATAATCAGTTAATCAGGAAAAAGAGCAAATAAAA
CAGAAAAGGAGTGAAAAAAT

Downstream 100 bases:

>100_bases
AAAAGAAAATGTCAAATAGGGACAAGACATTCTGGGCAGTTGTGATACCTGTTGTGGTTTTATTTTTCGCATTTAACACG
CTGCCGATGATAAAGGGTGT

Product: sugar ABC transporter substrate-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MAKRKICQAMAVVLALGMCTTGLTGCGNEKRADGKTEIEVVSYKPEAVKAFEKIEKQFNETHDDIHLTISSPNEAMTILK
TRFIREDYPDIIAIGGDINYSNFLDADLFEDISDLDAVDNVKEAYLDMDKELEFIPKDGTYALPYAANAAGVLYNKDMFA
ENGWKVPTTWSEFTALCDEIKESGTLPLYLGFKDTWTCLAPWNALAVGLCDSDTCNQVNMGNTTFEEAYSPVADKIRTLL
DYAEDNPYAYSYNDACTAFARGESAMYTIGSYAIPQIKSVNPDMNIGSFTFPANDNEADNVLNSGIDLQLSVMKACKNKE
AAYEVLEYLYSDETIQTYLDDQGGIACKEGDFAIPDTLEDMQEYIKDNRMSDYQDHHYPSEMSVDAMIQTYLLDTGDNAK
EKFLKKFDSDWERYNRDLIREVQDYQKEQEDAK

Sequences:

>Translated_433_residues
MAKRKICQAMAVVLALGMCTTGLTGCGNEKRADGKTEIEVVSYKPEAVKAFEKIEKQFNETHDDIHLTISSPNEAMTILK
TRFIREDYPDIIAIGGDINYSNFLDADLFEDISDLDAVDNVKEAYLDMDKELEFIPKDGTYALPYAANAAGVLYNKDMFA
ENGWKVPTTWSEFTALCDEIKESGTLPLYLGFKDTWTCLAPWNALAVGLCDSDTCNQVNMGNTTFEEAYSPVADKIRTLL
DYAEDNPYAYSYNDACTAFARGESAMYTIGSYAIPQIKSVNPDMNIGSFTFPANDNEADNVLNSGIDLQLSVMKACKNKE
AAYEVLEYLYSDETIQTYLDDQGGIACKEGDFAIPDTLEDMQEYIKDNRMSDYQDHHYPSEMSVDAMIQTYLLDTGDNAK
EKFLKKFDSDWERYNRDLIREVQDYQKEQEDAK
>Mature_432_residues
AKRKICQAMAVVLALGMCTTGLTGCGNEKRADGKTEIEVVSYKPEAVKAFEKIEKQFNETHDDIHLTISSPNEAMTILKT
RFIREDYPDIIAIGGDINYSNFLDADLFEDISDLDAVDNVKEAYLDMDKELEFIPKDGTYALPYAANAAGVLYNKDMFAE
NGWKVPTTWSEFTALCDEIKESGTLPLYLGFKDTWTCLAPWNALAVGLCDSDTCNQVNMGNTTFEEAYSPVADKIRTLLD
YAEDNPYAYSYNDACTAFARGESAMYTIGSYAIPQIKSVNPDMNIGSFTFPANDNEADNVLNSGIDLQLSVMKACKNKEA
AYEVLEYLYSDETIQTYLDDQGGIACKEGDFAIPDTLEDMQEYIKDNRMSDYQDHHYPSEMSVDAMIQTYLLDTGDNAKE
KFLKKFDSDWERYNRDLIREVQDYQKEQEDAK

Specific function: Involved in a binding protein-dependent transport system responsible for the uptake of melibiose, raffinose and isomaltotriose [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 48828; Mature: 48696

Theoretical pI: Translated: 4.06; Mature: 4.06

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRKICQAMAVVLALGMCTTGLTGCGNEKRADGKTEIEVVSYKPEAVKAFEKIEKQFNE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
THDDIHLTISSPNEAMTILKTRFIREDYPDIIAIGGDINYSNFLDADLFEDISDLDAVDN
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
VKEAYLDMDKELEFIPKDGTYALPYAANAAGVLYNKDMFAENGWKVPTTWSEFTALCDEI
HHHHHHCCCCCCEEECCCCCEECCCCCCCCCEEECCCHHCCCCCCCCCCHHHHHHHHHHH
KESGTLPLYLGFKDTWTCLAPWNALAVGLCDSDTCNQVNMGNTTFEEAYSPVADKIRTLL
HHCCCCEEEECCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DYAEDNPYAYSYNDACTAFARGESAMYTIGSYAIPQIKSVNPDMNIGSFTFPANDNEADN
HHHCCCCEEEECCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCHHHH
VLNSGIDLQLSVMKACKNKEAAYEVLEYLYSDETIQTYLDDQGGIACKEGDFAIPDTLED
HHHCCCCEEHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCHHHHH
MQEYIKDNRMSDYQDHHYPSEMSVDAMIQTYLLDTGDNAKEKFLKKFDSDWERYNRDLIR
HHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EVQDYQKEQEDAK
HHHHHHHHHHCCC
>Mature Secondary Structure 
AKRKICQAMAVVLALGMCTTGLTGCGNEKRADGKTEIEVVSYKPEAVKAFEKIEKQFNE
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCC
THDDIHLTISSPNEAMTILKTRFIREDYPDIIAIGGDINYSNFLDADLFEDISDLDAVDN
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
VKEAYLDMDKELEFIPKDGTYALPYAANAAGVLYNKDMFAENGWKVPTTWSEFTALCDEI
HHHHHHCCCCCCEEECCCCCEECCCCCCCCCEEECCCHHCCCCCCCCCCHHHHHHHHHHH
KESGTLPLYLGFKDTWTCLAPWNALAVGLCDSDTCNQVNMGNTTFEEAYSPVADKIRTLL
HHCCCCEEEECCCCCEEEECCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DYAEDNPYAYSYNDACTAFARGESAMYTIGSYAIPQIKSVNPDMNIGSFTFPANDNEADN
HHHCCCCEEEECCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEECCCCCCHHHH
VLNSGIDLQLSVMKACKNKEAAYEVLEYLYSDETIQTYLDDQGGIACKEGDFAIPDTLED
HHHCCCCEEHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCHHHHH
MQEYIKDNRMSDYQDHHYPSEMSVDAMIQTYLLDTGDNAKEKFLKKFDSDWERYNRDLIR
HHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EVQDYQKEQEDAK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1537846; 12397186 [H]