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The map label for this gene is punA [H]

Identifier: 238922722

GI number: 238922722

Start: 278279

End: 279112

Strand: Direct

Name: punA [H]

Synonym: EUBREC_0297

Alternate gene names: 238922722

Gene position: 278279-279112 (Clockwise)

Preceding gene: 238922721

Following gene: 238922723

Centisome position: 8.07

GC content: 48.44

Gene sequence:

>834_bases
ATGGAGAAAAAAATGAACAGTATATATGAAAAACTTACCACATGCCTTGCAAGTGTGCGTGAAAAAACAGATTTTGTACC
GGAGACAGCCATAGTTTTGGGCTCGGGACTTGGAGACTATGCAGAGCAGATTAAGATAGAGACAACCATAGATTACAAGG
ATATCAAGGGCTTTCCAACATCCACAGTTCCCGGACACAAAGGCAGGTTTGTATTTGGATATGTGGATAGTGTGCCTGTT
GTCATCATGCAGGGCAGAGTCCATTATTATGAGGGGTATCCGATAACAGACGTTGTGCTACCGACACGCCTCATGGGAAT
GATGGGCGCAAAAAAGCTGATACTCACAAATGCGGCAGGTGGTCTCAATACTGATTTTAATCCGGGAGACTTTATGCTGA
TTTCGGACCATATCGCGACAGCCATACCAAGTCCACTGATCGGAGCCAATATTGATGAGCTGGGCGAGCGTTTTCCGGAT
ATGAGCGAGGTATACAGCCGCAGAATGAGGGAAATCGTAAAAGAAAAGGCAGATAAGCTGGGCATAAAGCTGAGAGAAGG
AGTCTACGTACAGCTCACAGGCCCGCAGTATGAGACTCCGGCCGAGGTCAGGATGTGTAAAATCCTCGGAGGAGATGCCG
TCGGAATGAGCACAGCCTGTGAGGCACTTGCAGCGCGTCATATGGGGCTTGAGGTCTGCGGAATCTCATGTATCACCAAC
CTGGCAGCAGGACTATCCGACAAAAAGCTCAACCACAAGGAAGTGCAGGAAACCGCAGACCGCGTGGCAAAGCAGTTTAC
AGAACTGATCACTGCTGTGGTGAGAGCAGTGTAA

Upstream 100 bases:

>100_bases
TTCGGCGTAAAAATGCCGGAGGGTACCATCGGACATTCTATTCTTGAAGAGCTTTAAGGGGTAATATCTCAGGAATTTTT
TAAACTTTAACAGAAAATAT

Downstream 100 bases:

>100_bases
ATGCTTAGAAACTTGACAATAAATCGTATCATAGCGTATACTTTAGAATCCTAACAGATGGGATGGAGGATTTCAATGTT
AAATATATATTTAGGGGATA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEKKMNSIYEKLTTCLASVREKTDFVPETAIVLGSGLGDYAEQIKIETTIDYKDIKGFPTSTVPGHKGRFVFGYVDSVPV
VIMQGRVHYYEGYPITDVVLPTRLMGMMGAKKLILTNAAGGLNTDFNPGDFMLISDHIATAIPSPLIGANIDELGERFPD
MSEVYSRRMREIVKEKADKLGIKLREGVYVQLTGPQYETPAEVRMCKILGGDAVGMSTACEALAARHMGLEVCGISCITN
LAAGLSDKKLNHKEVQETADRVAKQFTELITAVVRAV

Sequences:

>Translated_277_residues
MEKKMNSIYEKLTTCLASVREKTDFVPETAIVLGSGLGDYAEQIKIETTIDYKDIKGFPTSTVPGHKGRFVFGYVDSVPV
VIMQGRVHYYEGYPITDVVLPTRLMGMMGAKKLILTNAAGGLNTDFNPGDFMLISDHIATAIPSPLIGANIDELGERFPD
MSEVYSRRMREIVKEKADKLGIKLREGVYVQLTGPQYETPAEVRMCKILGGDAVGMSTACEALAARHMGLEVCGISCITN
LAAGLSDKKLNHKEVQETADRVAKQFTELITAVVRAV
>Mature_277_residues
MEKKMNSIYEKLTTCLASVREKTDFVPETAIVLGSGLGDYAEQIKIETTIDYKDIKGFPTSTVPGHKGRFVFGYVDSVPV
VIMQGRVHYYEGYPITDVVLPTRLMGMMGAKKLILTNAAGGLNTDFNPGDFMLISDHIATAIPSPLIGANIDELGERFPD
MSEVYSRRMREIVKEKADKLGIKLREGVYVQLTGPQYETPAEVRMCKILGGDAVGMSTACEALAARHMGLEVCGISCITN
LAAGLSDKKLNHKEVQETADRVAKQFTELITAVVRAV

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=276, Percent_Identity=41.6666666666667, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1788746, Length=256, Percent_Identity=42.578125, Blast_Score=220, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI17541190, Length=278, Percent_Identity=39.2086330935252, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323238, Length=279, Percent_Identity=39.7849462365591, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=40.7272727272727, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=40.7272727272727, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24656093, Length=275, Percent_Identity=40.7272727272727, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI24656090, Length=275, Percent_Identity=40.7272727272727, Blast_Score=194, Evalue=8e-50,
Organism=Drosophila melanogaster, GI24762376, Length=244, Percent_Identity=31.5573770491803, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 30300; Mature: 30300

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKMNSIYEKLTTCLASVREKTDFVPETAIVLGSGLGDYAEQIKIETTIDYKDIKGFPT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHEEEEEEECHHHCCCCCC
STVPGHKGRFVFGYVDSVPVVIMQGRVHYYEGYPITDVVLPTRLMGMMGAKKLILTNAAG
CCCCCCCCCEEEEECCCCCEEEEECEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GLNTDFNPGDFMLISDHIATAIPSPLIGANIDELGERFPDMSEVYSRRMREIVKEKADKL
CCCCCCCCCCEEEECCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GIKLREGVYVQLTGPQYETPAEVRMCKILGGDAVGMSTACEALAARHMGLEVCGISCITN
CCEEECCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LAAGLSDKKLNHKEVQETADRVAKQFTELITAVVRAV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKKMNSIYEKLTTCLASVREKTDFVPETAIVLGSGLGDYAEQIKIETTIDYKDIKGFPT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHEEEEEEECHHHCCCCCC
STVPGHKGRFVFGYVDSVPVVIMQGRVHYYEGYPITDVVLPTRLMGMMGAKKLILTNAAG
CCCCCCCCCEEEEECCCCCEEEEECEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GLNTDFNPGDFMLISDHIATAIPSPLIGANIDELGERFPDMSEVYSRRMREIVKEKADKL
CCCCCCCCCCEEEECCHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
GIKLREGVYVQLTGPQYETPAEVRMCKILGGDAVGMSTACEALAARHMGLEVCGISCITN
CCEEECCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHH
LAAGLSDKKLNHKEVQETADRVAKQFTELITAVVRAV
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]