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The map label for this gene is glgB [H]

Identifier: 238922453

GI number: 238922453

Start: 33103

End: 35076

Strand: Direct

Name: glgB [H]

Synonym: EUBREC_0027

Alternate gene names: 238922453

Gene position: 33103-35076 (Clockwise)

Preceding gene: 238922452

Following gene: 238922454

Centisome position: 0.96

GC content: 44.07

Gene sequence:

>1974_bases
ATGGGGGGTATATATATGTGTAATTTTACAGCAATGGATTGTTACCTGTTTGGTCAGGCAACACACTATGATATCTACAA
GAAGATGGGTGCTCACTTAGCTGAGCGTAAAGGAAAAAAAGGAGTGTGCTTTGACGTCTGGGCGCCACATGCGAGGGAAG
TATACGTGTTTGGTGAGTTTAACGACTGGAATGAGACCAGTCATGAGATGAAGCGTGTAGAGCCGGAAACCATGGGAGTG
TATGAGCTTTTTGTACCGGGAGCGCAAAAGGGTCAGATGTATAAGTTTATTATAGTCACTGAACAGGGTGATAAGCTGTA
CAAGGCGGATCCATACGCAAACTACGCAGAGCTTCGTCCGGGAACAGCGTCAATCATCACAGATATCGAGCACTTCACAT
GGACTGACAAAGAGTGGATGGATAAGAGAGCAGCAAAGTCAGATGCTGATGTATATTCTTCACCTATGGCTATATATGAG
TGTCATCCGGGCTCATGGATGAGACATCCGGGCAGGGATGATGAGGGCTTTTATACATACAGGGAGCTTGCAAAATCACT
CATATCCTATGTGAAAACTATGGGATATACACATATTGAGCTTATGGGTATTTCAGAGTATCCGTTTGATGGCTCATGGG
GATATCAGGTGACAGGCTACTATGCACCTACATCGAGGTATGGAGAGCCTGAGGATTTTGCATACTTTGTTGATCAGTGC
CATAAAAATAATATCGGAGTAATCCTCGACTGGGTACCTGCACATTTCCCAAAGGATGCGCATGGACTTGCCAATTTCGA
TGGAACAGCCACATATGAGTATGCAGACCCAAGAAAGGGAGAGCATCCCGACTGGGGCACAAAGATATTTGACTACGGTA
AGAATGAAGTAAAGAACTTCCTTATCGGAAGTGCACTTATGTGGATAGAAAATTACCACGTAGACGGACTCCGTGTGGAT
GCAGTTGCGTCAATGCTTTATCTTGACTATGGAAAGAACGACGGACAGTGGGTGCCAAACAAGTTTGGAGGAAATAAGAA
CCTCGAGGCAGTAGAGTTTTTCAAGCATATCAATACACTCATACTTGGAAGAAATCACGGAACTGTAATGATAGCCGAAG
AGTCTACCGCATGGCCAAAGGTAACAGGCAAGGTAGAGGAAGACGGACTGAACTTCAGCTACAAATGGAACATGGGATGG
ATGCATGACTTCCTTGATTATATGAAGCTGGATCCATATTTCCGTAAAAATAACCACAACAAGATGACATTTGCGATGAG
CTATAATGAGAGTGAGAAATATATTCTGGTACTGTCACATGATGAGGTGGTACATCTGAAGTGTTCTATGCTCAACAAAA
TGCCGGGCTTAGAAGGGGATAAATTCAAGAACCTCATGGCAGGATATGCATTTATGATGGGACACTGTGGAAAGAAGCTT
CTTTTCATGGGACAGGAGTTTGCACAGAAGCAGGAGTGGAGTGAGGAAAGAGAACTCGACTGGTATCTGCTTGAGAATCC
GGAGCATAAAAAGATCCAGAACTGGGTAAAGGAGCTGCTTCACCTCTATAGGAGAAACCGCTGTCTGTATGAGCTGGATT
CAAGCTGGGAAGGCTTTGAATGGATAAATGCAAACGATGCAGACAGAAGCATCTTCAGCTTTATCAGAAAGAGTAAGGAT
GGCAAGAATAATATGCTCTTTGTCATCAATTTCACACCGGTAGAGAGACCTGATTACAGAGTCGGAGTGCCAAAGCACAA
GACATATCAGCTCGTTTTAGATAGTGAGGATCCTAAGTTTACCGGCAAAACGGTACAAGCGCCTGTAAAGAAGGCTGCAA
AGGGCAGAGGCAAGGCAGCAAAACAGTCATATAAGAAGCTGGACTATAAGGCAGTCAAATCAGAGTGTGACGGCAGACCA
TTTTCATTTGCTTATCCACTTGCGCCTTACGGAGTTGCTGTATTTAAATACTAA

Upstream 100 bases:

>100_bases
CGGACTTAAGAAGGCTATAAGTATTCTGACAGCCAGGCGATATACAGATGATGACCGAATTGTACTCAAAGAGGATCATT
TCAGGGAAAAATAAATACTT

Downstream 100 bases:

>100_bases
TATACCGGTAAACAGCAGGGTAAAGGACCGCGCCAATTTAAGGCGCGGTCTTTATTTTTGCGTATTATATGTCGATATAT
AATAAAAAGATAACTATTCA

Product: 1,4-alpha-glucan branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MGGIYMCNFTAMDCYLFGQATHYDIYKKMGAHLAERKGKKGVCFDVWAPHAREVYVFGEFNDWNETSHEMKRVEPETMGV
YELFVPGAQKGQMYKFIIVTEQGDKLYKADPYANYAELRPGTASIITDIEHFTWTDKEWMDKRAAKSDADVYSSPMAIYE
CHPGSWMRHPGRDDEGFYTYRELAKSLISYVKTMGYTHIELMGISEYPFDGSWGYQVTGYYAPTSRYGEPEDFAYFVDQC
HKNNIGVILDWVPAHFPKDAHGLANFDGTATYEYADPRKGEHPDWGTKIFDYGKNEVKNFLIGSALMWIENYHVDGLRVD
AVASMLYLDYGKNDGQWVPNKFGGNKNLEAVEFFKHINTLILGRNHGTVMIAEESTAWPKVTGKVEEDGLNFSYKWNMGW
MHDFLDYMKLDPYFRKNNHNKMTFAMSYNESEKYILVLSHDEVVHLKCSMLNKMPGLEGDKFKNLMAGYAFMMGHCGKKL
LFMGQEFAQKQEWSEERELDWYLLENPEHKKIQNWVKELLHLYRRNRCLYELDSSWEGFEWINANDADRSIFSFIRKSKD
GKNNMLFVINFTPVERPDYRVGVPKHKTYQLVLDSEDPKFTGKTVQAPVKKAAKGRGKAAKQSYKKLDYKAVKSECDGRP
FSFAYPLAPYGVAVFKY

Sequences:

>Translated_657_residues
MGGIYMCNFTAMDCYLFGQATHYDIYKKMGAHLAERKGKKGVCFDVWAPHAREVYVFGEFNDWNETSHEMKRVEPETMGV
YELFVPGAQKGQMYKFIIVTEQGDKLYKADPYANYAELRPGTASIITDIEHFTWTDKEWMDKRAAKSDADVYSSPMAIYE
CHPGSWMRHPGRDDEGFYTYRELAKSLISYVKTMGYTHIELMGISEYPFDGSWGYQVTGYYAPTSRYGEPEDFAYFVDQC
HKNNIGVILDWVPAHFPKDAHGLANFDGTATYEYADPRKGEHPDWGTKIFDYGKNEVKNFLIGSALMWIENYHVDGLRVD
AVASMLYLDYGKNDGQWVPNKFGGNKNLEAVEFFKHINTLILGRNHGTVMIAEESTAWPKVTGKVEEDGLNFSYKWNMGW
MHDFLDYMKLDPYFRKNNHNKMTFAMSYNESEKYILVLSHDEVVHLKCSMLNKMPGLEGDKFKNLMAGYAFMMGHCGKKL
LFMGQEFAQKQEWSEERELDWYLLENPEHKKIQNWVKELLHLYRRNRCLYELDSSWEGFEWINANDADRSIFSFIRKSKD
GKNNMLFVINFTPVERPDYRVGVPKHKTYQLVLDSEDPKFTGKTVQAPVKKAAKGRGKAAKQSYKKLDYKAVKSECDGRP
FSFAYPLAPYGVAVFKY
>Mature_656_residues
GGIYMCNFTAMDCYLFGQATHYDIYKKMGAHLAERKGKKGVCFDVWAPHAREVYVFGEFNDWNETSHEMKRVEPETMGVY
ELFVPGAQKGQMYKFIIVTEQGDKLYKADPYANYAELRPGTASIITDIEHFTWTDKEWMDKRAAKSDADVYSSPMAIYEC
HPGSWMRHPGRDDEGFYTYRELAKSLISYVKTMGYTHIELMGISEYPFDGSWGYQVTGYYAPTSRYGEPEDFAYFVDQCH
KNNIGVILDWVPAHFPKDAHGLANFDGTATYEYADPRKGEHPDWGTKIFDYGKNEVKNFLIGSALMWIENYHVDGLRVDA
VASMLYLDYGKNDGQWVPNKFGGNKNLEAVEFFKHINTLILGRNHGTVMIAEESTAWPKVTGKVEEDGLNFSYKWNMGWM
HDFLDYMKLDPYFRKNNHNKMTFAMSYNESEKYILVLSHDEVVHLKCSMLNKMPGLEGDKFKNLMAGYAFMMGHCGKKLL
FMGQEFAQKQEWSEERELDWYLLENPEHKKIQNWVKELLHLYRRNRCLYELDSSWEGFEWINANDADRSIFSFIRKSKDG
KNNMLFVINFTPVERPDYRVGVPKHKTYQLVLDSEDPKFTGKTVQAPVKKAAKGRGKAAKQSYKKLDYKAVKSECDGRPF
SFAYPLAPYGVAVFKY

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Transfers chain

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=622, Percent_Identity=26.8488745980707, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1789839, Length=596, Percent_Identity=47.6510067114094, Blast_Score=579, Evalue=1e-166,
Organism=Caenorhabditis elegans, GI17554896, Length=646, Percent_Identity=26.7801857585139, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI32564391, Length=611, Percent_Identity=26.3502454991817, Blast_Score=171, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6320826, Length=640, Percent_Identity=26.40625, Blast_Score=173, Evalue=9e-44,
Organism=Drosophila melanogaster, GI28573410, Length=650, Percent_Identity=27.6923076923077, Blast_Score=207, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 76192; Mature: 76061

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGIYMCNFTAMDCYLFGQATHYDIYKKMGAHLAERKGKKGVCFDVWAPHAREVYVFGEF
CCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECC
NDWNETSHEMKRVEPETMGVYELFVPGAQKGQMYKFIIVTEQGDKLYKADPYANYAELRP
CCCCHHHHHHHHCCCHHCCEEEEECCCCCCCCEEEEEEEECCCCCEEECCCCCCHHHCCC
GTASIITDIEHFTWTDKEWMDKRAAKSDADVYSSPMAIYECHPGSWMRHPGRDDEGFYTY
CCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCHHHH
RELAKSLISYVKTMGYTHIELMGISEYPFDGSWGYQVTGYYAPTSRYGEPEDFAYFVDQC
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
HKNNIGVILDWVPAHFPKDAHGLANFDGTATYEYADPRKGEHPDWGTKIFDYGKNEVKNF
CCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHCCHHHHHHH
LIGSALMWIENYHVDGLRVDAVASMLYLDYGKNDGQWVPNKFGGNKNLEAVEFFKHINTL
HHHHHEEEHHHCCCCCEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCEE
ILGRNHGTVMIAEESTAWPKVTGKVEEDGLNFSYKWNMGWMHDFLDYMKLDPYFRKNNHN
EEECCCCEEEEEECCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHCCCHHCCCCCC
KMTFAMSYNESEKYILVLSHDEVVHLKCSMLNKMPGLEGDKFKNLMAGYAFMMGHCGKKL
EEEEEEEECCCCCEEEEEECCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHH
LFMGQEFAQKQEWSEERELDWYLLENPEHKKIQNWVKELLHLYRRNRCLYELDSSWEGFE
HHHCHHHHHHHHCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE
WINANDADRSIFSFIRKSKDGKNNMLFVINFTPVERPDYRVGVPKHKTYQLVLDSEDPKF
EECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCCCEEEEEEECCCCCC
TGKTVQAPVKKAAKGRGKAAKQSYKKLDYKAVKSECDGRPFSFAYPLAPYGVAVFKY
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEECC
>Mature Secondary Structure 
GGIYMCNFTAMDCYLFGQATHYDIYKKMGAHLAERKGKKGVCFDVWAPHAREVYVFGEF
CCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECC
NDWNETSHEMKRVEPETMGVYELFVPGAQKGQMYKFIIVTEQGDKLYKADPYANYAELRP
CCCCHHHHHHHHCCCHHCCEEEEECCCCCCCCEEEEEEEECCCCCEEECCCCCCHHHCCC
GTASIITDIEHFTWTDKEWMDKRAAKSDADVYSSPMAIYECHPGSWMRHPGRDDEGFYTY
CCHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHCCCEEEEEECCCCCCCCCCCCCCCHHHH
RELAKSLISYVKTMGYTHIELMGISEYPFDGSWGYQVTGYYAPTSRYGEPEDFAYFVDQC
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
HKNNIGVILDWVPAHFPKDAHGLANFDGTATYEYADPRKGEHPDWGTKIFDYGKNEVKNF
CCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHCCHHHHHHH
LIGSALMWIENYHVDGLRVDAVASMLYLDYGKNDGQWVPNKFGGNKNLEAVEFFKHINTL
HHHHHEEEHHHCCCCCEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCEE
ILGRNHGTVMIAEESTAWPKVTGKVEEDGLNFSYKWNMGWMHDFLDYMKLDPYFRKNNHN
EEECCCCEEEEEECCCCCCCCCCCEECCCCCEEEEECCCHHHHHHHHHHCCCHHCCCCCC
KMTFAMSYNESEKYILVLSHDEVVHLKCSMLNKMPGLEGDKFKNLMAGYAFMMGHCGKKL
EEEEEEEECCCCCEEEEEECCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHH
LFMGQEFAQKQEWSEERELDWYLLENPEHKKIQNWVKELLHLYRRNRCLYELDSSWEGFE
HHHCHHHHHHHHCCHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE
WINANDADRSIFSFIRKSKDGKNNMLFVINFTPVERPDYRVGVPKHKTYQLVLDSEDPKF
EECCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCEECCCCCCEEEEEEECCCCCC
TGKTVQAPVKKAAKGRGKAAKQSYKKLDYKAVKSECDGRPFSFAYPLAPYGVAVFKY
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1938880 [H]