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The map label for this gene is purR [H]

Identifier: 238922295

GI number: 238922295

Start: 494868

End: 495887

Strand: Reverse

Name: purR [H]

Synonym: EUBELI_20531

Alternate gene names: 238922295

Gene position: 495887-494868 (Counterclockwise)

Preceding gene: 238922298

Following gene: 238922294

Centisome position: 79.12

GC content: 38.92

Gene sequence:

>1020_bases
ATGCATGAAAGGAATGAATCTATGGTATCTATGAAAGATGTTGCTGTAGAATGTGGGGTTTCAATTGCTACAGTAAGTAA
AGCTCTCAATGACCATAGTGACATCAGTGAAGAGACACGCAGGAAAGTTAAAGAGGCAGCTAACAGACTTGGATATTATC
CGAATTCAGCAGCCAGAGCACTTAAGACGAACCGTTCATATAACATAGGAGTTCTTCTTAATAATGGTCTTGCACATGAG
TATTTTGCCGAGGTTCTTGAGAGTTTTAAGAATGAAGCAGAGAAGAAGGGGTATGATATTACATTTGTAAGCAATCACTC
TAAGAAGATGACTTTCTACGAACACTGTTTATACAGGAATTTTGATGGCGTGCTTGTAGCTTGTGAGGATTTCAGCAATC
CTGAAATCTATGAAATGGTTAACGGGAGATTACCAGTTGTGACCATCGACTACATTTTCAATGGCAGTACATCGGTCAAT
TCTAACAACGTGAAAGGGATGAGTGAATTAGTACGTTACGCGTACAGCAGGGGACACAGAAAGATAGCTTATATTTATGG
AAACGTTGAATCAGAGGTTACGAAAGAACGTCTGGCAGCCTTTTATAAGACAATGGGTGAATTAGGGGTAGAAGTTCCAG
ATGAATACATTAAAGAATCAACCTATCTTGATTCGATAAAAGCTGAGAAGCTTACATATGAGCTGTTAAAATTAAAGGAA
CCGCCAACATGTATTTTTTATCCAGATGATTTATCTGTTGTTGGGGGCAAGAATGCTATTAAGAATATGAAATTGCATAG
TCCGAAGAATGTGTCTGTTGCAGGTTACGATGGAACAAGATTCTCGCAGATGTTAAATCCTAAGATTACAACAATTCGTC
AGAACACCAGGGAGATCGGCAAGATTGCCGCAGATGAGCTTATAAGTGCTATAGAGAATCCCAGGGCAACGATAGTAAAG
AGGGTTGTTGTAGATGCGGAACTAATCGAAGGGGAAACAATTAAAAAGATAGGAAACTAA

Upstream 100 bases:

>100_bases
AAAAAATACAATGATATATAAGAAAATAAGTAAAATTTACAAAAGTGCTTGCATTTATATCTGATATGAGTTATAGTAAA
GCTACGAAAACGATTAAGTA

Downstream 100 bases:

>100_bases
AGGTGTCGTCTAAGATTTATGGAGGGTTTTTTATGAAAAAGAGATTATTAAGTCTGGCACTTGGCACAATGATGGTGCTT
TCCACATTAGCAGGTTGTGG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MHERNESMVSMKDVAVECGVSIATVSKALNDHSDISEETRRKVKEAANRLGYYPNSAARALKTNRSYNIGVLLNNGLAHE
YFAEVLESFKNEAEKKGYDITFVSNHSKKMTFYEHCLYRNFDGVLVACEDFSNPEIYEMVNGRLPVVTIDYIFNGSTSVN
SNNVKGMSELVRYAYSRGHRKIAYIYGNVESEVTKERLAAFYKTMGELGVEVPDEYIKESTYLDSIKAEKLTYELLKLKE
PPTCIFYPDDLSVVGGKNAIKNMKLHSPKNVSVAGYDGTRFSQMLNPKITTIRQNTREIGKIAADELISAIENPRATIVK
RVVVDAELIEGETIKKIGN

Sequences:

>Translated_339_residues
MHERNESMVSMKDVAVECGVSIATVSKALNDHSDISEETRRKVKEAANRLGYYPNSAARALKTNRSYNIGVLLNNGLAHE
YFAEVLESFKNEAEKKGYDITFVSNHSKKMTFYEHCLYRNFDGVLVACEDFSNPEIYEMVNGRLPVVTIDYIFNGSTSVN
SNNVKGMSELVRYAYSRGHRKIAYIYGNVESEVTKERLAAFYKTMGELGVEVPDEYIKESTYLDSIKAEKLTYELLKLKE
PPTCIFYPDDLSVVGGKNAIKNMKLHSPKNVSVAGYDGTRFSQMLNPKITTIRQNTREIGKIAADELISAIENPRATIVK
RVVVDAELIEGETIKKIGN
>Mature_339_residues
MHERNESMVSMKDVAVECGVSIATVSKALNDHSDISEETRRKVKEAANRLGYYPNSAARALKTNRSYNIGVLLNNGLAHE
YFAEVLESFKNEAEKKGYDITFVSNHSKKMTFYEHCLYRNFDGVLVACEDFSNPEIYEMVNGRLPVVTIDYIFNGSTSVN
SNNVKGMSELVRYAYSRGHRKIAYIYGNVESEVTKERLAAFYKTMGELGVEVPDEYIKESTYLDSIKAEKLTYELLKLKE
PPTCIFYPDDLSVVGGKNAIKNMKLHSPKNVSVAGYDGTRFSQMLNPKITTIRQNTREIGKIAADELISAIENPRATIVK
RVVVDAELIEGETIKKIGN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=315, Percent_Identity=31.4285714285714, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=28.1437125748503, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=27.8996865203762, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=25.9146341463415, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=25.5520504731861, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1789068, Length=299, Percent_Identity=25.4180602006689, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI48994940, Length=323, Percent_Identity=26.3157894736842, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1786540, Length=321, Percent_Identity=25.5451713395639, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789202, Length=291, Percent_Identity=23.3676975945017, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787906, Length=336, Percent_Identity=22.3214285714286, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789456, Length=308, Percent_Identity=25, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=24.2990654205607, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38135; Mature: 38135

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHERNESMVSMKDVAVECGVSIATVSKALNDHSDISEETRRKVKEAANRLGYYPNSAARA
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
LKTNRSYNIGVLLNNGLAHEYFAEVLESFKNEAEKKGYDITFVSNHSKKMTFYEHCLYRN
HHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCC
FDGVLVACEDFSNPEIYEMVNGRLPVVTIDYIFNGSTSVNSNNVKGMSELVRYAYSRGHR
CCCEEEEECCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
KIAYIYGNVESEVTKERLAAFYKTMGELGVEVPDEYIKESTYLDSIKAEKLTYELLKLKE
EEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPTCIFYPDDLSVVGGKNAIKNMKLHSPKNVSVAGYDGTRFSQMLNPKITTIRQNTREIG
CCEEEECCCCCEECCCCHHHHCCCCCCCCCCEEECCCCHHHHHHCCCHHHHHHHHHHHHH
KIAADELISAIENPRATIVKRVVVDAELIEGETIKKIGN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MHERNESMVSMKDVAVECGVSIATVSKALNDHSDISEETRRKVKEAANRLGYYPNSAARA
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
LKTNRSYNIGVLLNNGLAHEYFAEVLESFKNEAEKKGYDITFVSNHSKKMTFYEHCLYRN
HHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCC
FDGVLVACEDFSNPEIYEMVNGRLPVVTIDYIFNGSTSVNSNNVKGMSELVRYAYSRGHR
CCCEEEEECCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCC
KIAYIYGNVESEVTKERLAAFYKTMGELGVEVPDEYIKESTYLDSIKAEKLTYELLKLKE
EEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPTCIFYPDDLSVVGGKNAIKNMKLHSPKNVSVAGYDGTRFSQMLNPKITTIRQNTREIG
CCEEEECCCCCEECCCCHHHHCCCCCCCCCCEEECCCCHHHHHHCCCHHHHHHHHHHHHH
KIAADELISAIENPRATIVKRVVVDAELIEGETIKKIGN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA