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The map label for this gene is pcrA [H]

Identifier: 238922029

GI number: 238922029

Start: 216239

End: 218392

Strand: Reverse

Name: pcrA [H]

Synonym: EUBELI_20264

Alternate gene names: 238922029

Gene position: 218392-216239 (Counterclockwise)

Preceding gene: 238922030

Following gene: 238922027

Centisome position: 34.85

GC content: 39.93

Gene sequence:

>2154_bases
ATGGAGAGATACCAAAGGTATACTAAATATTTACAGGTAAAGAGGACTATGAACATACCAGAAAGAGCAACGAAGGAACA
GACAGAGGCAATAACACAGACAGAAGGCAACATAAGGGTTGCGTCAGTTCCAGGTTCGGGAAAAACATTTGTACTTACAT
ATAGAATAGCATATCTTATAACAGAACTGTTCGTTGAGCCATCATCTATTGTGGCGCTTACATTTACAAACAAGGCAGCT
TCGCAGATGAAGCACAGGTTAAGGAATATTGTTGGTGATAATGCCAACTGTTTTACGGGAACATTTCACGGATATTGTAA
CATGTTTCTTAAAGAGGAGATTCACAGGCTGACATTTCCCAAGACATTCACGATTCTTGATAAGAAGGCAGAGCTGGAGA
TGATTAAGGACGTGGCAGAGGATATGGGAATATCGCTCAAGGACAATACTGCGAAGAAGATAATGAGTGATATAGACATA
ACCAAGCAGGATATGTCCTATGTTGAGCTTATGGCAGGCGTTGATAAGACAGAGCTTAAAAGAAGGGCTTCCAGCGAGAA
AAACGTGGATAAAAAGGTTTTCTATAATTATCTTAAGAGACAGTGTGACAATTATGCTCTGGACTTTGAAGACCTTATTA
ATTTCACTTTATATATTTTAAACAGGAATGAAGATGTGCGGATTCGCTGGCAGGAAAAATGCCAGTATGTACTGTGCGAC
GAGTATCAGGATGTCAATATGAAACAGGATATGCTGCTTCACATTCTAAGCGGTTTGTATCAGAATCTGTTCGTGGTAGG
TGACGACGACCAGAATATATATACATGGCGTGGTTCTGACCCTGAATACATGATTAATTTTGACAAAACACATGAAAATC
TGCAGGATTATTCACTTACTGAGAATTTCAGGAGTACTCCTCAGATTGTAAATGCAGCGAAATCCCTTATCTCTGCTAAT
CAGAACAGGCTTGAAAAGCAGATGATTTCTAACAGACCAGATGGCAATAAGCCGGTATTCAATGCAGCTGACACGGAGGA
GAAAGAGTCTTTGTGGATAGCTGATACTATTCTTGATAATGTCGCTAAGAATATGAAGTACAGCGACCATACTATATTGG
TGCGGGCTGCTTCACAGACAAGGTCGTTAGAGGAAGCATTTATAAAAAGAAAAGTTCCTTACAAGATACTAAGCGGTGCG
AAATTCTATGAAAGCGAAGAGATAAGAACGGTGCTTTCATACATGAGAATGGTGTACAGTCTTACGGACATGGATTTGCT
GTATACTATATCCAGACCGAGAAGGGGCTTTGGTAAGAAGTCTGTTGAGAAGCTTAAAAATGCAGCAGCACAGAACAACT
GCAGTCTTTTCAAGGCACTTGGAAAGCAGATTGAAGATGGCGATATAACACAGAAAAATGTTATAGAATATTACAATGCA
ATCAGTGAATTGCATGATACATATGTTACTTATACCTGTACTGATATAGTAAACAAATGTCTTGATATGGGATATCGTAA
GGCGTTGGAGCAGGACATCGACCAGACCAGACTTGATAATGTGTCAGAGCTTATCAGGACTATTACGGCACTGGAGGAAG
ATAATCAGGAAAAGGTTGAGCTGGCTGATCTGTTAAGCCATTTTGCATTATTTTCAGCGCAAGATGAGGACGGAGAGTAT
AATGTTGTAAAAGTAATGACAATTCATACAGCAAAAGGACTTGAGTTTGATACAGTATTTGTTCCAGGACTCGTTGAAGG
ACAGTTTCCAAGCAGCAGGCTTCGTAATGAAGACGAATATGAAGAGGAAAGAAGATTGTTGTATGTTGCAATGACGAGGG
CTAAGAATATGCTTTACCTCAGCACTTATAGAAAGAAAGATGGCCGTTTCGCTGCAAGACCATCAGCATTTTTAAGTGAC
ATTGATACTAATCTTCTTGACTGTATCAACAACAGCAGAGTATTAATCAGAGGTGTGACTGAGCAGATGCTGCCAAAGGC
CGTATTTGAGGTTGGAGATAAAGTCAGACATAAGGTATTTGGAACTGGCGAGATTGTTAAGGTAGATAAGGTTACACAGA
CATATGAAATCCAGTTTGAGAATATCAGGGGAACAAGAAGACTGATGTTTCGGGCAGAGCTGGGGAGATGTTGA

Upstream 100 bases:

>100_bases
GTTGCAGGATTTCAGGATATCCATACCGGTAAGATAGAGGAGATAATGCTGATTAAGCAGACTGGGGATATAGATACATT
TAAACAAATGTATGGAATTG

Downstream 100 bases:

>100_bases
AAATTAAAGCAGGTTAGAGAATGGAAAAATAGGGGAACCCCCTATTTTTTCATTCTTGACTGCAGAAAAGTAGGAATGAC
AAGTTCATTTTCTTTCTTTT

Product: DNA helicase II / ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MERYQRYTKYLQVKRTMNIPERATKEQTEAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPSSIVALTFTNKAA
SQMKHRLRNIVGDNANCFTGTFHGYCNMFLKEEIHRLTFPKTFTILDKKAELEMIKDVAEDMGISLKDNTAKKIMSDIDI
TKQDMSYVELMAGVDKTELKRRASSEKNVDKKVFYNYLKRQCDNYALDFEDLINFTLYILNRNEDVRIRWQEKCQYVLCD
EYQDVNMKQDMLLHILSGLYQNLFVVGDDDQNIYTWRGSDPEYMINFDKTHENLQDYSLTENFRSTPQIVNAAKSLISAN
QNRLEKQMISNRPDGNKPVFNAADTEEKESLWIADTILDNVAKNMKYSDHTILVRAASQTRSLEEAFIKRKVPYKILSGA
KFYESEEIRTVLSYMRMVYSLTDMDLLYTISRPRRGFGKKSVEKLKNAAAQNNCSLFKALGKQIEDGDITQKNVIEYYNA
ISELHDTYVTYTCTDIVNKCLDMGYRKALEQDIDQTRLDNVSELIRTITALEEDNQEKVELADLLSHFALFSAQDEDGEY
NVVKVMTIHTAKGLEFDTVFVPGLVEGQFPSSRLRNEDEYEEERRLLYVAMTRAKNMLYLSTYRKKDGRFAARPSAFLSD
IDTNLLDCINNSRVLIRGVTEQMLPKAVFEVGDKVRHKVFGTGEIVKVDKVTQTYEIQFENIRGTRRLMFRAELGRC

Sequences:

>Translated_717_residues
MERYQRYTKYLQVKRTMNIPERATKEQTEAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPSSIVALTFTNKAA
SQMKHRLRNIVGDNANCFTGTFHGYCNMFLKEEIHRLTFPKTFTILDKKAELEMIKDVAEDMGISLKDNTAKKIMSDIDI
TKQDMSYVELMAGVDKTELKRRASSEKNVDKKVFYNYLKRQCDNYALDFEDLINFTLYILNRNEDVRIRWQEKCQYVLCD
EYQDVNMKQDMLLHILSGLYQNLFVVGDDDQNIYTWRGSDPEYMINFDKTHENLQDYSLTENFRSTPQIVNAAKSLISAN
QNRLEKQMISNRPDGNKPVFNAADTEEKESLWIADTILDNVAKNMKYSDHTILVRAASQTRSLEEAFIKRKVPYKILSGA
KFYESEEIRTVLSYMRMVYSLTDMDLLYTISRPRRGFGKKSVEKLKNAAAQNNCSLFKALGKQIEDGDITQKNVIEYYNA
ISELHDTYVTYTCTDIVNKCLDMGYRKALEQDIDQTRLDNVSELIRTITALEEDNQEKVELADLLSHFALFSAQDEDGEY
NVVKVMTIHTAKGLEFDTVFVPGLVEGQFPSSRLRNEDEYEEERRLLYVAMTRAKNMLYLSTYRKKDGRFAARPSAFLSD
IDTNLLDCINNSRVLIRGVTEQMLPKAVFEVGDKVRHKVFGTGEIVKVDKVTQTYEIQFENIRGTRRLMFRAELGRC
>Mature_717_residues
MERYQRYTKYLQVKRTMNIPERATKEQTEAITQTEGNIRVASVPGSGKTFVLTYRIAYLITELFVEPSSIVALTFTNKAA
SQMKHRLRNIVGDNANCFTGTFHGYCNMFLKEEIHRLTFPKTFTILDKKAELEMIKDVAEDMGISLKDNTAKKIMSDIDI
TKQDMSYVELMAGVDKTELKRRASSEKNVDKKVFYNYLKRQCDNYALDFEDLINFTLYILNRNEDVRIRWQEKCQYVLCD
EYQDVNMKQDMLLHILSGLYQNLFVVGDDDQNIYTWRGSDPEYMINFDKTHENLQDYSLTENFRSTPQIVNAAKSLISAN
QNRLEKQMISNRPDGNKPVFNAADTEEKESLWIADTILDNVAKNMKYSDHTILVRAASQTRSLEEAFIKRKVPYKILSGA
KFYESEEIRTVLSYMRMVYSLTDMDLLYTISRPRRGFGKKSVEKLKNAAAQNNCSLFKALGKQIEDGDITQKNVIEYYNA
ISELHDTYVTYTCTDIVNKCLDMGYRKALEQDIDQTRLDNVSELIRTITALEEDNQEKVELADLLSHFALFSAQDEDGEY
NVVKVMTIHTAKGLEFDTVFVPGLVEGQFPSSRLRNEDEYEEERRLLYVAMTRAKNMLYLSTYRKKDGRFAARPSAFLSD
IDTNLLDCINNSRVLIRGVTEQMLPKAVFEVGDKVRHKVFGTGEIVKVDKVTQTYEIQFENIRGTRRLMFRAELGRC

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=700, Percent_Identity=30.1428571428571, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI48994965, Length=647, Percent_Identity=29.984544049459, Blast_Score=259, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6322369, Length=460, Percent_Identity=30.2173913043478, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324477, Length=494, Percent_Identity=24.4939271255061, Blast_Score=97, Evalue=7e-21,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83004; Mature: 83004

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYQRYTKYLQVKRTMNIPERATKEQTEAITQTEGNIRVASVPGSGKTFVLTYRIAYLI
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHHH
TELFVEPSSIVALTFTNKAASQMKHRLRNIVGDNANCFTGTFHGYCNMFLKEEIHRLTFP
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHCCC
KTFTILDKKAELEMIKDVAEDMGISLKDNTAKKIMSDIDITKQDMSYVELMAGVDKTELK
CEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHH
RRASSEKNVDKKVFYNYLKRQCDNYALDFEDLINFTLYILNRNEDVRIRWQEKCQYVLCD
HHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCEEEEEEECCCCEEEEEHHCCCEEEEC
EYQDVNMKQDMLLHILSGLYQNLFVVGDDDQNIYTWRGSDPEYMINFDKTHENLQDYSLT
CCCCCCCHHHHHHHHHHHHHHCEEEEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHH
ENFRSTPQIVNAAKSLISANQNRLEKQMISNRPDGNKPVFNAADTEEKESLWIADTILDN
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHH
VAKNMKYSDHTILVRAASQTRSLEEAFIKRKVPYKILSGAKFYESEEIRTVLSYMRMVYS
HHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
LTDMDLLYTISRPRRGFGKKSVEKLKNAAAQNNCSLFKALGKQIEDGDITQKNVIEYYNA
HCCCHHEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
ISELHDTYVTYTCTDIVNKCLDMGYRKALEQDIDQTRLDNVSELIRTITALEEDNQEKVE
HHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LADLLSHFALFSAQDEDGEYNVVKVMTIHTAKGLEFDTVFVPGLVEGQFPSSRLRNEDEY
HHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCEECEEECCCCCCCCCCHHHCCCCCHH
EEERRLLYVAMTRAKNMLYLSTYRKKDGRFAARPSAFLSDIDTNLLDCINNSRVLIRGVT
HHHHHEEHEEEHHCCCEEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCH
EQMLPKAVFEVGDKVRHKVFGTGEIVKVDKVTQTYEIQFENIRGTRRLMFRAELGRC
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MERYQRYTKYLQVKRTMNIPERATKEQTEAITQTEGNIRVASVPGSGKTFVLTYRIAYLI
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEHHHHHHHHH
TELFVEPSSIVALTFTNKAASQMKHRLRNIVGDNANCFTGTFHGYCNMFLKEEIHRLTFP
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHCCC
KTFTILDKKAELEMIKDVAEDMGISLKDNTAKKIMSDIDITKQDMSYVELMAGVDKTELK
CEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHH
RRASSEKNVDKKVFYNYLKRQCDNYALDFEDLINFTLYILNRNEDVRIRWQEKCQYVLCD
HHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCEEEEEEECCCCEEEEEHHCCCEEEEC
EYQDVNMKQDMLLHILSGLYQNLFVVGDDDQNIYTWRGSDPEYMINFDKTHENLQDYSLT
CCCCCCCHHHHHHHHHHHHHHCEEEEECCCCEEEEEECCCCCEEEEECCCCCCHHHHHHH
ENFRSTPQIVNAAKSLISANQNRLEKQMISNRPDGNKPVFNAADTEEKESLWIADTILDN
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHH
VAKNMKYSDHTILVRAASQTRSLEEAFIKRKVPYKILSGAKFYESEEIRTVLSYMRMVYS
HHHHCCCCCCEEEEEECHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
LTDMDLLYTISRPRRGFGKKSVEKLKNAAAQNNCSLFKALGKQIEDGDITQKNVIEYYNA
HCCCHHEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
ISELHDTYVTYTCTDIVNKCLDMGYRKALEQDIDQTRLDNVSELIRTITALEEDNQEKVE
HHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LADLLSHFALFSAQDEDGEYNVVKVMTIHTAKGLEFDTVFVPGLVEGQFPSSRLRNEDEY
HHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCEECEEECCCCCCCCCCHHHCCCCCHH
EEERRLLYVAMTRAKNMLYLSTYRKKDGRFAARPSAFLSDIDTNLLDCINNSRVLIRGVT
HHHHHEEHEEEHHCCCEEEEEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCH
EQMLPKAVFEVGDKVRHKVFGTGEIVKVDKVTQTYEIQFENIRGTRRLMFRAELGRC
HHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]