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The map label for this gene is nodI [H]

Identifier: 238922014

GI number: 238922014

Start: 204783

End: 205481

Strand: Reverse

Name: nodI [H]

Synonym: EUBELI_20249

Alternate gene names: 238922014

Gene position: 205481-204783 (Counterclockwise)

Preceding gene: 238922015

Following gene: 238922013

Centisome position: 32.79

GC content: 25.75

Gene sequence:

>699_bases
ATGATAGAAATAAATAATTTAAGTAAGTCTTATGGTATAAATAAAATTTTTGAAAATGAGAGCATTACATTAACTAATTC
CTGTATATATGCTTTAGTAGGGGTAAATGGTATTGGAAAATCGACTTTTTTAAATTCGATTATCCAACCTAGCACATTGG
ATAAAGGAAGTGTAAAAATAGATGATATTCCTTCTACCGATTTTACAGCAAAATATCACTATGCTTTTGTTCCAGATACA
AAGGATATGTTTTTAAATTTGACTGGAAAAGAATATTTACAATTTGTTAGTGAACTTTATAATCAAAAAGAAAAGTTTGA
TGAACTGCTTGCTATCTATATACCAAAATTGAAGTTGGAAAATTCATTAGAACAAACTATTTCCAGTTATTCTCTTGGAA
TGAAACAAAAAATATATCTTGCAGGAGCTTTCATGTCAAATGCTCAAAACATTATTTTGGACGAACCGTTTAATGCGATT
GACCCAGAAAGTACTTCCATTATCAAAAAAATATTATTTGATTTAAAAGATACTGGAAAAATGATTTTATTTTCTGTTCA
TAATTTAGATTTAGTATCAAATTTCTGTGATAAAATTATTTTTATAGATAACAGACATAGCATTTTTGAGTGTAACAACC
CACAAAACTTTGATGTATTAGAAAAAATATTTTTTGACAAATGTGTAATAAAAAAATAA

Upstream 100 bases:

>100_bases
AAATTTTCCTTTGTTAAATTTTCTATTGGATTATCAATGATGAATATCTTGATTTTATTATTGATAGGTGTGGTTATGTA
TATGAAAAGAGGTCTTTCAG

Downstream 100 bases:

>100_bases
ATTTAATGACTTAGTGCTAGAATACTGGGAGAATTTTATGAAATGAATGAGAAAGAACTAACTTCTAGTTTTAAGGCTCT
CTCTTGCGAAACTAGAAGAA

Product: ABC-2 type transport system ATP-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: Nodulation ATP-binding protein I [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIEINNLSKSYGINKIFENESITLTNSCIYALVGVNGIGKSTFLNSIIQPSTLDKGSVKIDDIPSTDFTAKYHYAFVPDT
KDMFLNLTGKEYLQFVSELYNQKEKFDELLAIYIPKLKLENSLEQTISSYSLGMKQKIYLAGAFMSNAQNIILDEPFNAI
DPESTSIIKKILFDLKDTGKMILFSVHNLDLVSNFCDKIIFIDNRHSIFECNNPQNFDVLEKIFFDKCVIKK

Sequences:

>Translated_232_residues
MIEINNLSKSYGINKIFENESITLTNSCIYALVGVNGIGKSTFLNSIIQPSTLDKGSVKIDDIPSTDFTAKYHYAFVPDT
KDMFLNLTGKEYLQFVSELYNQKEKFDELLAIYIPKLKLENSLEQTISSYSLGMKQKIYLAGAFMSNAQNIILDEPFNAI
DPESTSIIKKILFDLKDTGKMILFSVHNLDLVSNFCDKIIFIDNRHSIFECNNPQNFDVLEKIFFDKCVIKK
>Mature_232_residues
MIEINNLSKSYGINKIFENESITLTNSCIYALVGVNGIGKSTFLNSIIQPSTLDKGSVKIDDIPSTDFTAKYHYAFVPDT
KDMFLNLTGKEYLQFVSELYNQKEKFDELLAIYIPKLKLENSLEQTISSYSLGMKQKIYLAGAFMSNAQNIILDEPFNAI
DPESTSIIKKILFDLKDTGKMILFSVHNLDLVSNFCDKIIFIDNRHSIFECNNPQNFDVLEKIFFDKCVIKK

Specific function: Part of the ABC transporter complex NodIJ involved in the export of the nodulation factors (Nod factors), the bacterial signal molecules that induce symbiosis and subsequent nodulation induction. Nod factors are LCO (lipo-chitin oligosaccharide), a modifi

COG id: COG1131

COG function: function code V; ABC-type multidrug transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI45446740, Length=213, Percent_Identity=27.6995305164319, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI47078218, Length=213, Percent_Identity=27.6995305164319, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI31657092, Length=222, Percent_Identity=25.2252252252252, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI105990541, Length=188, Percent_Identity=27.1276595744681, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21536376, Length=213, Percent_Identity=27.2300469483568, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI27881501, Length=218, Percent_Identity=23.8532110091743, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI30795238, Length=218, Percent_Identity=23.8532110091743, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787029, Length=222, Percent_Identity=25.6756756756757, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789593, Length=214, Percent_Identity=27.1028037383178, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790739, Length=217, Percent_Identity=23.0414746543779, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788450, Length=226, Percent_Identity=23.8938053097345, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787105, Length=220, Percent_Identity=25, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786563, Length=228, Percent_Identity=25, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI87082268, Length=219, Percent_Identity=26.027397260274, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786398, Length=220, Percent_Identity=26.8181818181818, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789672, Length=224, Percent_Identity=22.3214285714286, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1789751, Length=248, Percent_Identity=26.2096774193548, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786319, Length=217, Percent_Identity=27.1889400921659, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48995001, Length=218, Percent_Identity=25.2293577981651, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081782, Length=221, Percent_Identity=22.1719457013575, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786872, Length=210, Percent_Identity=22.3809523809524, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789991, Length=218, Percent_Identity=26.605504587156, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=197, Percent_Identity=25.3807106598985, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=221, Percent_Identity=25.3393665158371, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=221, Percent_Identity=25.3393665158371, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=221, Percent_Identity=25.3393665158371, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=208, Percent_Identity=23.5576923076923, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=198, Percent_Identity=22.7272727272727, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17569143, Length=189, Percent_Identity=31.7460317460317, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17569145, Length=189, Percent_Identity=32.2751322751323, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24666092, Length=218, Percent_Identity=28.4403669724771, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28574150, Length=219, Percent_Identity=22.8310502283105, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI116007328, Length=219, Percent_Identity=22.8310502283105, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648314, Length=210, Percent_Identity=29.0476190476191, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI221512771, Length=208, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28573571, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28573573, Length=204, Percent_Identity=26.9607843137255, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24643674, Length=204, Percent_Identity=23.0392156862745, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR015851
- InterPro:   IPR005978
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26437; Mature: 26437

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEINNLSKSYGINKIFENESITLTNSCIYALVGVNGIGKSTFLNSIIQPSTLDKGSVKI
CEEECCCHHHCCCCCEECCCCEEEEHHHEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEE
DDIPSTDFTAKYHYAFVPDTKDMFLNLTGKEYLQFVSELYNQKEKFDELLAIYIPKLKLE
CCCCCCCCEEEEEEEECCCCHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NSLEQTISSYSLGMKQKIYLAGAFMSNAQNIILDEPFNAIDPESTSIIKKILFDLKDTGK
HHHHHHHHHHHCCCCEEEEEEEHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
MILFSVHNLDLVSNFCDKIIFIDNRHSIFECNNPQNFDVLEKIFFDKCVIKK
EEEEEECCHHHHHHHHCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEINNLSKSYGINKIFENESITLTNSCIYALVGVNGIGKSTFLNSIIQPSTLDKGSVKI
CEEECCCHHHCCCCCEECCCCEEEEHHHEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEE
DDIPSTDFTAKYHYAFVPDTKDMFLNLTGKEYLQFVSELYNQKEKFDELLAIYIPKLKLE
CCCCCCCCEEEEEEEECCCCHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
NSLEQTISSYSLGMKQKIYLAGAFMSNAQNIILDEPFNAIDPESTSIIKKILFDLKDTGK
HHHHHHHHHHHCCCCEEEEEEEHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
MILFSVHNLDLVSNFCDKIIFIDNRHSIFECNNPQNFDVLEKIFFDKCVIKK
EEEEEECCHHHHHHHHCEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]