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The map label for this gene is mepA [H]

Identifier: 238921962

GI number: 238921962

Start: 158414

End: 159781

Strand: Reverse

Name: mepA [H]

Synonym: EUBELI_20197

Alternate gene names: 238921962

Gene position: 159781-158414 (Counterclockwise)

Preceding gene: 238921963

Following gene: 238921961

Centisome position: 25.49

GC content: 38.08

Gene sequence:

>1368_bases
ATGGCAGAAAGTAACAAGATGAGAGAGATGCCGGTAAATAAGCTCATGGTTCAGATGGGAATACCGATGATCTTATCTAT
GGCATTGCAAGCGGTTTACAATATTGTAGACAGTGCCTTTGTTGGAAACATGAGATCAGGAAGTGAGGCGGCACTTAATG
CACTTACACTGGTGTTTCCAGTTCAGATGCTCATGGTAGCAGTTGGAATCGGAACAGGTGTGGGAACAAATGCACTTCTT
GCAAGAACACTTGGACAGGGAAATGGTAAAAAAGCAGCAAAAGTTGCGGGAAACAGTTTGTTTCTTGGAGTGATTATTTA
TGTGGTGTGCCTGTTGTTTGGGATTTTTGGAGTAAGAGCATATATTTCATCACAGACAATTGACCCTGAAGTTATATTAA
TGGGAACAGATTACTTAAGAATATGCTGTGTAATTTCATTTGGAATTATTTTCTTTTCACTATTTGAAAAGCTGCTGCAG
GCAACCGGACGTTCTCTTTATTCTACAATTGGTCAGGTAGTTGGAGCAGTGGTAAATATTATTCTTGATCCTATTATGAT
TTATGGTATTGGACCGGTTCCTGAAATGGGAGTTAAGGGGGCTGCATATGCAACTGTTATTGGACAGGTTGCGTCTGCAA
TATTATTGTTTGTATTTCATATGAAACTGAATAAGGAATTTGAACATGATGTAAAGTATATGAAACCGGATAGCAGAATA
ATCAAGGAAATATATGCAATTGGACTTCCTGCAATTATTGCACAGGCACTTATGTCTATCATGGTATATGTGATGAATCT
GATTTTGAAATTCAGCCCTTCAGCACAGACTGCATATGGATTGTTCTATAAAGTACAGCAGTTTGTATTGTTCCTTGCCT
TTGGGCTTAGAGATGCAATCACTCCGATTATTGCATTTGCTTATGGTATGAGAAGTAAAAAGAGAATTCAGGATGGGATC
AGGTATGGACTGCTCTATACCGTTGTACTAATGATTCTAGGAATTGCAATTACGGAAATTTTTCCTGGAGCTTTTGCAAC
TTTGTTCAATGCAGGACAGTCAAGAGAATATTTTATTGGAGCAATGAGGATAATCTCAATAAGCTTTCTGTTTGCTGGAA
TAAATGTTGCTTATCAGGGAATTTATCAGGCACTGGATGGCGGCGTTGAATCATTGGTTATCTCTCTTCTGAGACAGCTT
ATCATTATCCTGCCACTGTCTGGAATTTTTTCTGTGTTTGTCAGAAATGGTCAGATGGGAGTTTCTCTTATCTGGTGGGC
ATTCCCAATAACTGAGGTTGTTTCATGTCTGGCAGGATATGTATTTTTAAAGAGAATCAGAAAGAATAAAGTAGATGTTT
TAAATTAA

Upstream 100 bases:

>100_bases
TGAGATAAGTACACACTTTTTTTATGCAAAAAAAGGAGGCTGAATCCTCTCCTCACCAAAGGGGTCGGATTTTGCTTCGC
AAAACAAGGAGGATTATAAA

Downstream 100 bases:

>100_bases
GGAGGAATCGTAAATGGCAAAAAGGATTATTACAATCAGCAGGGAATTTGGAAGCGGTGGTCGATTTATCGGTGAAGAAG
TGGCAAAGAAACTTGGAGTT

Product: multidrug resistance protein, MATE family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MAESNKMREMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFPVQMLMVAVGIGTGVGTNALL
ARTLGQGNGKKAAKVAGNSLFLGVIIYVVCLLFGIFGVRAYISSQTIDPEVILMGTDYLRICCVISFGIIFFSLFEKLLQ
ATGRSLYSTIGQVVGAVVNIILDPIMIYGIGPVPEMGVKGAAYATVIGQVASAILLFVFHMKLNKEFEHDVKYMKPDSRI
IKEIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAITPIIAFAYGMRSKKRIQDGI
RYGLLYTVVLMILGIAITEIFPGAFATLFNAGQSREYFIGAMRIISISFLFAGINVAYQGIYQALDGGVESLVISLLRQL
IIILPLSGIFSVFVRNGQMGVSLIWWAFPITEVVSCLAGYVFLKRIRKNKVDVLN

Sequences:

>Translated_455_residues
MAESNKMREMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFPVQMLMVAVGIGTGVGTNALL
ARTLGQGNGKKAAKVAGNSLFLGVIIYVVCLLFGIFGVRAYISSQTIDPEVILMGTDYLRICCVISFGIIFFSLFEKLLQ
ATGRSLYSTIGQVVGAVVNIILDPIMIYGIGPVPEMGVKGAAYATVIGQVASAILLFVFHMKLNKEFEHDVKYMKPDSRI
IKEIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAITPIIAFAYGMRSKKRIQDGI
RYGLLYTVVLMILGIAITEIFPGAFATLFNAGQSREYFIGAMRIISISFLFAGINVAYQGIYQALDGGVESLVISLLRQL
IIILPLSGIFSVFVRNGQMGVSLIWWAFPITEVVSCLAGYVFLKRIRKNKVDVLN
>Mature_454_residues
AESNKMREMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFPVQMLMVAVGIGTGVGTNALLA
RTLGQGNGKKAAKVAGNSLFLGVIIYVVCLLFGIFGVRAYISSQTIDPEVILMGTDYLRICCVISFGIIFFSLFEKLLQA
TGRSLYSTIGQVVGAVVNIILDPIMIYGIGPVPEMGVKGAAYATVIGQVASAILLFVFHMKLNKEFEHDVKYMKPDSRII
KEIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAITPIIAFAYGMRSKKRIQDGIR
YGLLYTVVLMILGIAITEIFPGAFATLFNAGQSREYFIGAMRIISISFLFAGINVAYQGIYQALDGGVESLVISLLRQLI
IILPLSGIFSVFVRNGQMGVSLIWWAFPITEVVSCLAGYVFLKRIRKNKVDVLN

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=344, Percent_Identity=22.9651162790698, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49679; Mature: 49548

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESNKMREMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFP
CCCCCCCHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VQMLMVAVGIGTGVGTNALLARTLGQGNGKKAAKVAGNSLFLGVIIYVVCLLFGIFGVRA
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
YISSQTIDPEVILMGTDYLRICCVISFGIIFFSLFEKLLQATGRSLYSTIGQVVGAVVNI
HHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ILDPIMIYGIGPVPEMGVKGAAYATVIGQVASAILLFVFHMKLNKEFEHDVKYMKPDSRI
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHH
IKEIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGLFYKVQQFVLFLAFGLRDAI
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TPIIAFAYGMRSKKRIQDGIRYGLLYTVVLMILGIAITEIFPGAFATLFNAGQSREYFIG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHH
AMRIISISFLFAGINVAYQGIYQALDGGVESLVISLLRQLIIILPLSGIFSVFVRNGQMG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VSLIWWAFPITEVVSCLAGYVFLKRIRKNKVDVLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AESNKMREMPVNKLMVQMGIPMILSMALQAVYNIVDSAFVGNMRSGSEAALNALTLVFP
CCCCCCHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA