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The map label for this gene is cobD [H]

Identifier: 238921941

GI number: 238921941

Start: 139804

End: 140841

Strand: Reverse

Name: cobD [H]

Synonym: EUBELI_20175

Alternate gene names: 238921941

Gene position: 140841-139804 (Counterclockwise)

Preceding gene: 238921942

Following gene: 238921940

Centisome position: 22.47

GC content: 38.44

Gene sequence:

>1038_bases
ATGAATAATAATATTTTTACATGTATATTTACATGTCAGACTATAGCAATCATGTGTGGATATGTGCTTGATCTGATTAT
CGGAGATCCGCACTGGCTGTATCACCCGGTACGCCTGATAGGAAAAATGATTTCATGGCTGGAAAGCATTTTGTTAAAAG
AAGAGTATTCACAAGCAAAGAAATATAAAAGAGGCATAGTTCTGGCAGTTTTAACACCATTAATAACAGGTATTGTTACA
GCGGGCATACTTGCGGTCTGCTATTATATTAATATAGTGCTTGGATGTGTAGTCGAAACGATTATGTGCTATCAGATTCT
TGCTGTGAAATCACTAAAAACAGAGAGCATGAAAGTATATTATGCTTTGAAAAATGAAGGGATTCCACAGGCAAGACAGG
CTGTATCAATGATTGTCGGAAGAGATACCAGCCAGCTTGATGAACATGGTATTACGCGTGCGGCTGTTGAGACGGTTGCG
GAAAATACATCAGATGGTGTTGTTGCGCCATTGTTTTATATGATGTTTTTCGGTGCTGTCGGGGGCTTCGTATATAAGGC
AGTTAATACCATGGATTCAATGATTGGCTATAAGAATGATAAATATCTGCATTTCGGAAGATTTGCGGCTAAAATGGATG
ATGTTGTGAACTTTATTCCTGCAAGAGCAGGCGGATGTCTTATGGTTGCAGCTGCCGGAATTGCACAGCTTGCAGAAAAG
TGTATGGGAAGGAATAAAGATTTATCACATTACAGCATGGGAAATGCATGGAAGATTTTTTTAAGGGACAGAATGAAACA
CAGCAGTCCTAATTCCGCACAGACAGAATCGGCATGTGCAGGAGCATTGAAGGTTTCTCTGTCAGGAGACAATTATTATT
TTGGCAGACTTGTACATAAGCCGCAGATTGGAGATTCAATCCGGGAGCTGGAAATTGAAGATATAAAAAGAAGTAATATT
TTATTGTATATCACCGCATTTATTGTGATTATTATTGTGCTCATTATAAGAAGTGCAGTGATGTGTTATTTCTGTTAA

Upstream 100 bases:

>100_bases
GATACTATTATGATTATCATTGTGGCAATGGGGATGGATATATCTGCGATGTTACTGAGGAAGGACATATAAGTACAATA
AAAAAGATTACGGAGAAATA

Downstream 100 bases:

>100_bases
TCAGACGAAAGGTTATCATTTATGGAAAAACAGATACATGGCGGGAATATCTATGACAAGGAAGTGTCACTTGATTTTTC
GGTTAATATTAATCCGCTTG

Product: adenosylcobinamide-phosphate synthase CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MNNNIFTCIFTCQTIAIMCGYVLDLIIGDPHWLYHPVRLIGKMISWLESILLKEEYSQAKKYKRGIVLAVLTPLITGIVT
AGILAVCYYINIVLGCVVETIMCYQILAVKSLKTESMKVYYALKNEGIPQARQAVSMIVGRDTSQLDEHGITRAAVETVA
ENTSDGVVAPLFYMMFFGAVGGFVYKAVNTMDSMIGYKNDKYLHFGRFAAKMDDVVNFIPARAGGCLMVAAAGIAQLAEK
CMGRNKDLSHYSMGNAWKIFLRDRMKHSSPNSAQTESACAGALKVSLSGDNYYFGRLVHKPQIGDSIRELEIEDIKRSNI
LLYITAFIVIIIVLIIRSAVMCYFC

Sequences:

>Translated_345_residues
MNNNIFTCIFTCQTIAIMCGYVLDLIIGDPHWLYHPVRLIGKMISWLESILLKEEYSQAKKYKRGIVLAVLTPLITGIVT
AGILAVCYYINIVLGCVVETIMCYQILAVKSLKTESMKVYYALKNEGIPQARQAVSMIVGRDTSQLDEHGITRAAVETVA
ENTSDGVVAPLFYMMFFGAVGGFVYKAVNTMDSMIGYKNDKYLHFGRFAAKMDDVVNFIPARAGGCLMVAAAGIAQLAEK
CMGRNKDLSHYSMGNAWKIFLRDRMKHSSPNSAQTESACAGALKVSLSGDNYYFGRLVHKPQIGDSIRELEIEDIKRSNI
LLYITAFIVIIIVLIIRSAVMCYFC
>Mature_345_residues
MNNNIFTCIFTCQTIAIMCGYVLDLIIGDPHWLYHPVRLIGKMISWLESILLKEEYSQAKKYKRGIVLAVLTPLITGIVT
AGILAVCYYINIVLGCVVETIMCYQILAVKSLKTESMKVYYALKNEGIPQARQAVSMIVGRDTSQLDEHGITRAAVETVA
ENTSDGVVAPLFYMMFFGAVGGFVYKAVNTMDSMIGYKNDKYLHFGRFAAKMDDVVNFIPARAGGCLMVAAAGIAQLAEK
CMGRNKDLSHYSMGNAWKIFLRDRMKHSSPNSAQTESACAGALKVSLSGDNYYFGRLVHKPQIGDSIRELEIEDIKRSNI
LLYITAFIVIIIVLIIRSAVMCYFC

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 38337; Mature: 38337

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNIFTCIFTCQTIAIMCGYVLDLIIGDPHWLYHPVRLIGKMISWLESILLKEEYSQAK
CCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYKRGIVLAVLTPLITGIVTAGILAVCYYINIVLGCVVETIMCYQILAVKSLKTESMKVY
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YALKNEGIPQARQAVSMIVGRDTSQLDEHGITRAAVETVAENTSDGVVAPLFYMMFFGAV
EEECCCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GGFVYKAVNTMDSMIGYKNDKYLHFGRFAAKMDDVVNFIPARAGGCLMVAAAGIAQLAEK
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
CMGRNKDLSHYSMGNAWKIFLRDRMKHSSPNSAQTESACAGALKVSLSGDNYYFGRLVHK
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCEEEEEEEEC
PQIGDSIRELEIEDIKRSNILLYITAFIVIIIVLIIRSAVMCYFC
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNNIFTCIFTCQTIAIMCGYVLDLIIGDPHWLYHPVRLIGKMISWLESILLKEEYSQAK
CCCCEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYKRGIVLAVLTPLITGIVTAGILAVCYYINIVLGCVVETIMCYQILAVKSLKTESMKVY
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
YALKNEGIPQARQAVSMIVGRDTSQLDEHGITRAAVETVAENTSDGVVAPLFYMMFFGAV
EEECCCCCHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GGFVYKAVNTMDSMIGYKNDKYLHFGRFAAKMDDVVNFIPARAGGCLMVAAAGIAQLAEK
HHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
CMGRNKDLSHYSMGNAWKIFLRDRMKHSSPNSAQTESACAGALKVSLSGDNYYFGRLVHK
HCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEECCCCEEEEEEEEC
PQIGDSIRELEIEDIKRSNILLYITAFIVIIIVLIIRSAVMCYFC
CCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]