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The map label for this gene is nagE [H]

Identifier: 238921845

GI number: 238921845

Start: 59716

End: 61224

Strand: Reverse

Name: nagE [H]

Synonym: EUBELI_20078

Alternate gene names: 238921845

Gene position: 61224-59716 (Counterclockwise)

Preceding gene: 238921846

Following gene: 238921842

Centisome position: 9.77

GC content: 37.64

Gene sequence:

>1509_bases
ATGCGCGTGATTGGGATAACCCCGATTAATAATGATTTAAAGGAGGAGAAAAGTATGAAATTTCTTCAAAAACTGGGAAA
AGCGTTAATGCTTCCTGTGGCAGTACTGCCTATATGTGGCATCCTTATGGGTATCGGTTATATGCTGTGTCCAGAAACTA
TGCAGGGTGCAGCTAATATGATTGGTCTGTTTCTTGTAAAAGCTGGTGGAGCTTTAATTGATAACATGGCAATCCTTTTT
GTAATTGGTGTCGGTGTAGGTATGTCAGATAAAAATGATGGTACCGGAGGAATTGCGGCACTTGCTTCGTGGCTTATGAT
GACAACACTTCTTTCTACAAAGTTTGTTACAGTAATTATACCTTCTATAGCAGACAGTGCAACTAAAACTCTTGCATTTA
ACAAGATTGAGAATCCATTTATTGGTATTCTTGCTGGTATAATCGGTGCTATGTGTTATAACAAGTTTAAAGATACAAAA
TTACCAAACTGGTTATCTTTCTTTAGTGGAAAACGATGCGTAGCAATTGTTTCAGGAGTAGTATCAATTCTTGTATCTGT
TGTGTTATTATTTGTATGGCCATTACTGTTCGGCGCATTAGTAAGCGTTGGTAAGGCAATTGTAAAGCTTGATATTGTTG
GTGCAGGTATCTATGCATTCTTGAACAGATTGTTAATTCCTACCGGATTACATCATGCGTTGAACAACGTATTCTGGTTT
GATACTATCGGACTTGGAGATTTACAGCACTTCTGGGCAGGACAAACATCAAGTGATGTTTCATGGAGTCTTGGAATGTA
TATGTCAGGCTTCTTCCCATGTATGATGTTTGGTGTTCCTGGTGCAGCTTTAGCTATGATTAAATGTGCAAAGCCAGCTA
AGAAAAAAGTAGCTATAGGACTTGTTGCTTCTGCAGCATTATGTGCATTTATCTGTGGTGTTACAGAACCATTTGAATTT
GGTTTCATGTTCCTTGCTCCTGGACTCTATGTTATCTATGCACTTCTTTATGGTATATTTACAATGATTACAGTTGCATT
AGGCTTCAGAGCAGGATTTAGTTTCTCAGCAGGTGCTACTGACCTGTTATTCTCATCATCTCTTCCGGCAGCACAGAAGA
CATGGTTGATAATTCCTCTTGGTATAGCAGCATTCTTCGTATTCTATTTCGTATTCTTATTTGCCATTAAGAAATGGGAT
TTAAAGACTCCTGGCAGAGAAGATGATGATATAGAAGCAGAAAAGAAAGTTGAACTTGCAAGTGACGATTATACAGCAAT
TGCAAAGACAATTCTTGAAGGCTGTGGTGGAAAAGAGAATATTGCAAGCATAGATAATTGTATTACAAGACTGAGACTTG
AAGTAAAGGATATTACACTTGTTAATGATAAGGTTATTAAATCAGCTGGTGTTGCTGGTGTTATGAAACCTGGAAAGACA
TCTGTACAGGTTATTATAGGAACTAAGGTACAGTTTGTTGCGGATGCATTTTCTAAGCTTTGTGAATAA

Upstream 100 bases:

>100_bases
GCAATACCGACGATTATAATAAAATTAATCTGATAAAGGAGGGTGAGGTATCTATTGATGATGAAGTTCTGAAAATATCA
TAATTACTCAGGATTTCACA

Downstream 100 bases:

>100_bases
TTAAAATGTTAAAATAAAGATAATATAAAAATCCCCGGTAAGACTGTCAGAACAGCAGCTTATCGGGGATTATTTCATGT
GTTACTTTACCATGCTTAAA

Product: PTS system, N-acetylglucosamine-specific IIB component / PTS system, N-acetylglucosamine-specific IIC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MRVIGITPINNDLKEEKSMKFLQKLGKALMLPVAVLPICGILMGIGYMLCPETMQGAANMIGLFLVKAGGALIDNMAILF
VIGVGVGMSDKNDGTGGIAALASWLMMTTLLSTKFVTVIIPSIADSATKTLAFNKIENPFIGILAGIIGAMCYNKFKDTK
LPNWLSFFSGKRCVAIVSGVVSILVSVVLLFVWPLLFGALVSVGKAIVKLDIVGAGIYAFLNRLLIPTGLHHALNNVFWF
DTIGLGDLQHFWAGQTSSDVSWSLGMYMSGFFPCMMFGVPGAALAMIKCAKPAKKKVAIGLVASAALCAFICGVTEPFEF
GFMFLAPGLYVIYALLYGIFTMITVALGFRAGFSFSAGATDLLFSSSLPAAQKTWLIIPLGIAAFFVFYFVFLFAIKKWD
LKTPGREDDDIEAEKKVELASDDYTAIAKTILEGCGGKENIASIDNCITRLRLEVKDITLVNDKVIKSAGVAGVMKPGKT
SVQVIIGTKVQFVADAFSKLCE

Sequences:

>Translated_502_residues
MRVIGITPINNDLKEEKSMKFLQKLGKALMLPVAVLPICGILMGIGYMLCPETMQGAANMIGLFLVKAGGALIDNMAILF
VIGVGVGMSDKNDGTGGIAALASWLMMTTLLSTKFVTVIIPSIADSATKTLAFNKIENPFIGILAGIIGAMCYNKFKDTK
LPNWLSFFSGKRCVAIVSGVVSILVSVVLLFVWPLLFGALVSVGKAIVKLDIVGAGIYAFLNRLLIPTGLHHALNNVFWF
DTIGLGDLQHFWAGQTSSDVSWSLGMYMSGFFPCMMFGVPGAALAMIKCAKPAKKKVAIGLVASAALCAFICGVTEPFEF
GFMFLAPGLYVIYALLYGIFTMITVALGFRAGFSFSAGATDLLFSSSLPAAQKTWLIIPLGIAAFFVFYFVFLFAIKKWD
LKTPGREDDDIEAEKKVELASDDYTAIAKTILEGCGGKENIASIDNCITRLRLEVKDITLVNDKVIKSAGVAGVMKPGKT
SVQVIIGTKVQFVADAFSKLCE
>Mature_502_residues
MRVIGITPINNDLKEEKSMKFLQKLGKALMLPVAVLPICGILMGIGYMLCPETMQGAANMIGLFLVKAGGALIDNMAILF
VIGVGVGMSDKNDGTGGIAALASWLMMTTLLSTKFVTVIIPSIADSATKTLAFNKIENPFIGILAGIIGAMCYNKFKDTK
LPNWLSFFSGKRCVAIVSGVVSILVSVVLLFVWPLLFGALVSVGKAIVKLDIVGAGIYAFLNRLLIPTGLHHALNNVFWF
DTIGLGDLQHFWAGQTSSDVSWSLGMYMSGFFPCMMFGVPGAALAMIKCAKPAKKKVAIGLVASAALCAFICGVTEPFEF
GFMFLAPGLYVIYALLYGIFTMITVALGFRAGFSFSAGATDLLFSSSLPAAQKTWLIIPLGIAAFFVFYFVFLFAIKKWD
LKTPGREDDDIEAEKKVELASDDYTAIAKTILEGCGGKENIASIDNCITRLRLEVKDITLVNDKVIKSAGVAGVMKPGKT
SVQVIIGTKVQFVADAFSKLCE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=494, Percent_Identity=40.6882591093117, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1787343, Length=480, Percent_Identity=38.3333333333333, Blast_Score=288, Evalue=8e-79,
Organism=Escherichia coli, GI1787908, Length=519, Percent_Identity=34.6820809248555, Blast_Score=252, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 53798; Mature: 53798

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVIGITPINNDLKEEKSMKFLQKLGKALMLPVAVLPICGILMGIGYMLCPETMQGAANM
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IGLFLVKAGGALIDNMAILFVIGVGVGMSDKNDGTGGIAALASWLMMTTLLSTKFVTVII
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PSIADSATKTLAFNKIENPFIGILAGIIGAMCYNKFKDTKLPNWLSFFSGKRCVAIVSGV
CHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHH
VSILVSVVLLFVWPLLFGALVSVGKAIVKLDIVGAGIYAFLNRLLIPTGLHHALNNVFWF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEE
DTIGLGDLQHFWAGQTSSDVSWSLGMYMSGFFPCMMFGVPGAALAMIKCAKPAKKKVAIG
ECCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHH
LVASAALCAFICGVTEPFEFGFMFLAPGLYVIYALLYGIFTMITVALGFRAGFSFSAGAT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
DLLFSSSLPAAQKTWLIIPLGIAAFFVFYFVFLFAIKKWDLKTPGREDDDIEAEKKVELA
HHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCC
SDDYTAIAKTILEGCGGKENIASIDNCITRLRLEVKDITLVNDKVIKSAGVAGVMKPGKT
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEHHHHHHHCCCCCCCCCCCC
SVQVIIGTKVQFVADAFSKLCE
EEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure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EEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]