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The map label for this gene is thiDE [H]

Identifier: 238921815

GI number: 238921815

Start: 27903

End: 28259

Strand: Reverse

Name: thiDE [H]

Synonym: EUBELI_20048

Alternate gene names: 238921815

Gene position: 28259-27903 (Counterclockwise)

Preceding gene: 238921816

Following gene: 238921813

Centisome position: 4.51

GC content: 39.22

Gene sequence:

>357_bases
ATGACAGCTAATCGTGTATATGCGATGAGTGCAATCACAGCCCTGACTGCACAGAACACTACTTGTGTAACAGATATAAT
GGAGGTATCTCCTAAGTTTTTAGCAGAGCAGCTGGATGGTATATTTACAGATATATATCCGGATGCTGTCAAGACAGGAA
TGGTTGCTTCAGGTGAACTGATTCAGGTAATAGCAGATAAGCTTACAGAGTATAAAGCAGGAAATATAGTTGTTGATCCA
GTTATGGTGGCGACAAGTGGTGCAAGACTTATAAGTGAAGATGCTATCAGCATATTAAAGAGCAGATTATTACCACTTGC
AACAGTTATTACTAGAACATTCCACGACAGAATGTAA

Upstream 100 bases:

>100_bases
ACCAGACATTTACGTATAATGGGAAAAAGCTTGAACAGGCGGGGCTCATAGATTATATAAAGACTAAAAAGGTGCCGGAA
TACAGGCAGATATAAAGACA

Downstream 100 bases:

>100_bases
TCATACGAATAGTATAATTGCTTGAAATTAGAATGCAGTATTTAAGCGGTTTGGCAAAAAATGTATATACTAATACTTAA
TGTAAATTGGTGTAAACTAG

Product: hydroxymethylpyrimidine kinase / phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MTANRVYAMSAITALTAQNTTCVTDIMEVSPKFLAEQLDGIFTDIYPDAVKTGMVASGELIQVIADKLTEYKAGNIVVDP
VMVATSGARLISEDAISILKSRLLPLATVITRTFHDRM

Sequences:

>Translated_118_residues
MTANRVYAMSAITALTAQNTTCVTDIMEVSPKFLAEQLDGIFTDIYPDAVKTGMVASGELIQVIADKLTEYKAGNIVVDP
VMVATSGARLISEDAISILKSRLLPLATVITRTFHDRM
>Mature_117_residues
TANRVYAMSAITALTAQNTTCVTDIMEVSPKFLAEQLDGIFTDIYPDAVKTGMVASGELIQVIADKLTEYKAGNIVVDPV
MVATSGARLISEDAISILKSRLLPLATVITRTFHDRM

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=105, Percent_Identity=43.8095238095238, Blast_Score=98, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324997, Length=113, Percent_Identity=37.1681415929204, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324517, Length=113, Percent_Identity=35.3982300884956, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325378, Length=118, Percent_Identity=30.5084745762712, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 12751; Mature: 12620

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANRVYAMSAITALTAQNTTCVTDIMEVSPKFLAEQLDGIFTDIYPDAVKTGMVASGEL
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCHH
IQVIADKLTEYKAGNIVVDPVMVATSGARLISEDAISILKSRLLPLATVITRTFHDRM
HHHHHHHHHHCCCCCEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TANRVYAMSAITALTAQNTTCVTDIMEVSPKFLAEQLDGIFTDIYPDAVKTGMVASGEL
CCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHCCCCCCHH
IQVIADKLTEYKAGNIVVDPVMVATSGARLISEDAISILKSRLLPLATVITRTFHDRM
HHHHHHHHHHCCCCCEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA