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The map label for this gene is ldh [H]

Identifier: 238921793

GI number: 238921793

Start: 13540

End: 14490

Strand: Reverse

Name: ldh [H]

Synonym: EUBELI_20026

Alternate gene names: 238921793

Gene position: 14490-13540 (Counterclockwise)

Preceding gene: 238921794

Following gene: 238921792

Centisome position: 2.31

GC content: 38.7

Gene sequence:

>951_bases
ATGAAAGTTATTAATTCAAAAAAAGCAGTAATGATAGGCTGTGGCTTTGTTGGTTCTGCATCGGTATTTGCTCTGATGCA
GAGTGGCTTGTTTACAGAGATTGTCCTTATCGATGCAGATAAGAACAAGGCAGAAGGAGAAGCGATGGATATCAGTCATG
GTATTCCATTTGCGAGTCCGATGAAAATATATGCCGGAGATTATGATGATGTGGCTGATGCTGCAATTGTTGTCATATCT
GCCGGAGCGGGACAGAAGCCGGGAGAGACAAGGCTTGATCTCGTAAATAAAAATGTAGCAATATTTAAGTCAATTATTCC
TGAAATAGCAAAGAGAAATTTTGCAGGTATCATGCTTGTAGTTGCGAATCCGGTAGATATCCTGACTCAGGTAGCCATAA
AGTTATCAGGACTTCCAGAAAACAGGGTTATTGGATCGGGTACTGTGTTAGATAGTGCAAGATTAAGATATAAGCTTGGT
GAGCATTTATCTGTGGACAGCAGGAGTGTTCATGCATTTATAGTAGGTGAGCATGGAGACAGCGAAGTAGTAGTATGGTC
ATCAGCCAATGTATCTGGTGTTCCATTAAGTGAAATGTGTGAAATGCGAGGACATTACAAGCACAAGGAAAACACGGCAG
AAATAGCTACAGCAGTCAAGAACAGTGCTTATGAGATTATAAACAAAAAGCACGCTACATATTATGGAATTGCAATGTCT
GTAAAAAGAATTTGCGAAGTGATTATGAGAGATGAAAAATCAATACTTCCTGTATCACACATGATTCATGGAGTATATGA
TATTGACGGAGTATCGTTAAGTATGCCAGCTATTGTAGGTGCAGATGGTATTGAGTCTGATATACCTATTAATTTAAGTG
GTGAGGAAGCGCTAAAGCTTAAAGAATCCGCAGATTCTTTGAAAAAGATTATAGAGACAATTGAATTATAA

Upstream 100 bases:

>100_bases
CAGGGAGAAGATAAAGGAGGAAAAGTATGGATAGGAAAGCAATGTATAAGTTGAGTTATGGACTGTTTATATTGATTAAG
GAGATGAGATTATTACGAAG

Downstream 100 bases:

>100_bases
ATTTTACACATGGAAGTATATACAGTGGAAGGCAGTCATGTTCATGTCGTGGTTGGAGAGACAAAACATCCAATGCTTGA
AGAGCATTTCATTGAGTGGA

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKVINSKKAVMIGCGFVGSASVFALMQSGLFTEIVLIDADKNKAEGEAMDISHGIPFASPMKIYAGDYDDVADAAIVVIS
AGAGQKPGETRLDLVNKNVAIFKSIIPEIAKRNFAGIMLVVANPVDILTQVAIKLSGLPENRVIGSGTVLDSARLRYKLG
EHLSVDSRSVHAFIVGEHGDSEVVVWSSANVSGVPLSEMCEMRGHYKHKENTAEIATAVKNSAYEIINKKHATYYGIAMS
VKRICEVIMRDEKSILPVSHMIHGVYDIDGVSLSMPAIVGADGIESDIPINLSGEEALKLKESADSLKKIIETIEL

Sequences:

>Translated_316_residues
MKVINSKKAVMIGCGFVGSASVFALMQSGLFTEIVLIDADKNKAEGEAMDISHGIPFASPMKIYAGDYDDVADAAIVVIS
AGAGQKPGETRLDLVNKNVAIFKSIIPEIAKRNFAGIMLVVANPVDILTQVAIKLSGLPENRVIGSGTVLDSARLRYKLG
EHLSVDSRSVHAFIVGEHGDSEVVVWSSANVSGVPLSEMCEMRGHYKHKENTAEIATAVKNSAYEIINKKHATYYGIAMS
VKRICEVIMRDEKSILPVSHMIHGVYDIDGVSLSMPAIVGADGIESDIPINLSGEEALKLKESADSLKKIIETIEL
>Mature_316_residues
MKVINSKKAVMIGCGFVGSASVFALMQSGLFTEIVLIDADKNKAEGEAMDISHGIPFASPMKIYAGDYDDVADAAIVVIS
AGAGQKPGETRLDLVNKNVAIFKSIIPEIAKRNFAGIMLVVANPVDILTQVAIKLSGLPENRVIGSGTVLDSARLRYKLG
EHLSVDSRSVHAFIVGEHGDSEVVVWSSANVSGVPLSEMCEMRGHYKHKENTAEIATAVKNSAYEIINKKHATYYGIAMS
VKRICEVIMRDEKSILPVSHMIHGVYDIDGVSLSMPAIVGADGIESDIPINLSGEEALKLKESADSLKKIIETIEL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI5031857, Length=311, Percent_Identity=45.3376205787781, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI260099723, Length=311, Percent_Identity=45.3376205787781, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI47059044, Length=315, Percent_Identity=44.1269841269841, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI221136809, Length=315, Percent_Identity=44.1269841269841, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI15082234, Length=317, Percent_Identity=43.8485804416404, Blast_Score=255, Evalue=3e-68,
Organism=Homo sapiens, GI291575128, Length=309, Percent_Identity=44.6601941747573, Blast_Score=246, Evalue=1e-65,
Organism=Homo sapiens, GI4557032, Length=309, Percent_Identity=44.6601941747573, Blast_Score=246, Evalue=1e-65,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=44.5901639344262, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=44.5901639344262, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI260099727, Length=215, Percent_Identity=46.9767441860465, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI260099725, Length=210, Percent_Identity=47.6190476190476, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI207028494, Length=198, Percent_Identity=46.969696969697, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI103472011, Length=317, Percent_Identity=27.7602523659306, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI103472015, Length=186, Percent_Identity=30.6451612903226, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1789632, Length=282, Percent_Identity=26.241134751773, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=294, Percent_Identity=42.5170068027211, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17136226, Length=300, Percent_Identity=40.6666666666667, Blast_Score=226, Evalue=1e-59,
Organism=Drosophila melanogaster, GI45550422, Length=305, Percent_Identity=29.5081967213115, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24647881, Length=322, Percent_Identity=25.4658385093168, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.27 [H]

Molecular weight: Translated: 33910; Mature: 33910

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVINSKKAVMIGCGFVGSASVFALMQSGLFTEIVLIDADKNKAEGEAMDISHGIPFASP
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCC
MKIYAGDYDDVADAAIVVISAGAGQKPGETRLDLVNKNVAIFKSIIPEIAKRNFAGIMLV
CEEEECCCCHHCCEEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
VANPVDILTQVAIKLSGLPENRVIGSGTVLDSARLRYKLGEHLSVDSRSVHAFIVGEHGD
EECCHHHHHHHHHHHCCCCCCCEECCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
SEVVVWSSANVSGVPLSEMCEMRGHYKHKENTAEIATAVKNSAYEIINKKHATYYGIAMS
CCEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
VKRICEVIMRDEKSILPVSHMIHGVYDIDGVSLSMPAIVGADGIESDIPINLSGEEALKL
HHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEECCEEECCCCCCCCCCEEECCHHHHHH
KESADSLKKIIETIEL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVINSKKAVMIGCGFVGSASVFALMQSGLFTEIVLIDADKNKAEGEAMDISHGIPFASP
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEECCCCCCCCCC
MKIYAGDYDDVADAAIVVISAGAGQKPGETRLDLVNKNVAIFKSIIPEIAKRNFAGIMLV
CEEEECCCCHHCCEEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCEEEEE
VANPVDILTQVAIKLSGLPENRVIGSGTVLDSARLRYKLGEHLSVDSRSVHAFIVGEHGD
EECCHHHHHHHHHHHCCCCCCCEECCCCEEHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
SEVVVWSSANVSGVPLSEMCEMRGHYKHKENTAEIATAVKNSAYEIINKKHATYYGIAMS
CCEEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
VKRICEVIMRDEKSILPVSHMIHGVYDIDGVSLSMPAIVGADGIESDIPINLSGEEALKL
HHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEECCEEECCCCCCCCCCEEECCHHHHHH
KESADSLKKIIETIEL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA