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The map label for this gene is cmr [H]

Identifier: 238921696

GI number: 238921696

Start: 3736177

End: 3737307

Strand: Direct

Name: cmr [H]

Synonym: NT01EI_3857

Alternate gene names: 238921696

Gene position: 3736177-3737307 (Clockwise)

Preceding gene: 238921694

Following gene: 238921702

Centisome position: 98.0

GC content: 58.89

Gene sequence:

>1131_bases
ATGATTCAACCCGGCATGCTGTCAGTAGTACAAACCTTTGGCGTGGACGAAAGCTGGGTTCCGACTTCCATGACCGCCTA
TCTGGCCGGCGGCATGTTTCTGCAGTGGCTGCTCGGCCCGCTCTCCGATCGCATCGGGCGGCGACCGGTAATGTTAATCG
GCACGCTCTACTTTGCCGCAACCTGCCTGGCAATTTTGCTGACCAACAGCATCGAACAGTTCACCCTGATGCGTTTTTTA
CAAGGGATCAGCCTGTGCTTTATTGGCGCCGTCGGCTATGCCGCGATCCAGGAGTCCTTTGAGGAGTCCGTATGTATCAA
AATCACCGCGCTAATGGCCAACGTTGCGCTGATCGCGCCACTGCTCGGGCCATTAGCCGGCGCTGCATGGGTGCATCTGT
TTCCCTGGGAGGGCATGTTTATCCTGTTTGCCGCGCTGTCTCTGCTCGCCTTCCTTGGTCTGTATAAAGCCATGCCGGAA
ACCGCGACACGACGGGGAGAAAAGCTGTCACTGTCAGCACTGGGCCGCGACTATACGCTGGTGCTGAAAAATAGACGCTT
TCTGTGCGGCTCGCTGGCCTGCGGCTTCGCCAGCCTGCCTCTACTGGCCTGGATAGCCCAGTCGCCGGTGATCATCATCA
GCGGCGAAGGCCTCAGCAGCTATGACTACGGCATGCTACAGGTTCCAATTTTCGGCATGCTGATCCTGGGTAACCTTACC
CTGGCCCGCCTGAGCGGCCGCCGCCCGGTCCGCCGTCTGATTCAGCTGGGCGCCTGGCCAATGGTTGGCGGGCTGGCGAT
CGCCGCCGCCTCTACGCTATACAGCGCCCATGCCTACCTGTGGATGACGGCAGGCCTGAGTCTATATGCCTTTGGTATTG
GCCTGGCCAACGCCGGTCTCTATCGCCTGACGCTGTTTTCCAGCACGATGAGCAAGGGCACCGTCTCTGCTGCAATGGGC
ATGATCAGTATGTTCATCTATACCCTTGGCATTGAGGTAGGCAAATACGCCTGGCTGCTGGGTGGAAACGGCGCATTCAA
CCTGTTCAACCTGATAAGCGGCCTGCTGTGGCTGGCGCTGATCGCACGGATGCTGCGCGATCAGCTGGTGGGGAGGATGG
CGGGTAGATGA

Upstream 100 bases:

>100_bases
GCAAAACCATTTATCTTCTACTCGCCGACTGGGACGGCGTGCGTTGTTATTTCCCCTCTGTCTGGTGTTGTATGAGTTTG
CCACTTATATCGGAAACGAT

Downstream 100 bases:

>100_bases
GACAGCGGCGCAGACGTATGACAGGCTGTACGCCGCCATCGGCTTAAACCTGCAGCCAGAAAGTGACCGGACCGTCATTG
AGCAGGGAGACCTGCATATC

Product: multidrug resistance protein MdtM

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIQPGMLSVVQTFGVDESWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLIGTLYFAATCLAILLTNSIEQFTLMRFL
QGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAPLLGPLAGAAWVHLFPWEGMFILFAALSLLAFLGLYKAMPE
TATRRGEKLSLSALGRDYTLVLKNRRFLCGSLACGFASLPLLAWIAQSPVIIISGEGLSSYDYGMLQVPIFGMLILGNLT
LARLSGRRPVRRLIQLGAWPMVGGLAIAAASTLYSAHAYLWMTAGLSLYAFGIGLANAGLYRLTLFSSTMSKGTVSAAMG
MISMFIYTLGIEVGKYAWLLGGNGAFNLFNLISGLLWLALIARMLRDQLVGRMAGR

Sequences:

>Translated_376_residues
MIQPGMLSVVQTFGVDESWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLIGTLYFAATCLAILLTNSIEQFTLMRFL
QGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAPLLGPLAGAAWVHLFPWEGMFILFAALSLLAFLGLYKAMPE
TATRRGEKLSLSALGRDYTLVLKNRRFLCGSLACGFASLPLLAWIAQSPVIIISGEGLSSYDYGMLQVPIFGMLILGNLT
LARLSGRRPVRRLIQLGAWPMVGGLAIAAASTLYSAHAYLWMTAGLSLYAFGIGLANAGLYRLTLFSSTMSKGTVSAAMG
MISMFIYTLGIEVGKYAWLLGGNGAFNLFNLISGLLWLALIARMLRDQLVGRMAGR
>Mature_376_residues
MIQPGMLSVVQTFGVDESWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLIGTLYFAATCLAILLTNSIEQFTLMRFL
QGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAPLLGPLAGAAWVHLFPWEGMFILFAALSLLAFLGLYKAMPE
TATRRGEKLSLSALGRDYTLVLKNRRFLCGSLACGFASLPLLAWIAQSPVIIISGEGLSSYDYGMLQVPIFGMLILGNLT
LARLSGRRPVRRLIQLGAWPMVGGLAIAAASTLYSAHAYLWMTAGLSLYAFGIGLANAGLYRLTLFSSTMSKGTVSAAMG
MISMFIYTLGIEVGKYAWLLGGNGAFNLFNLISGLLWLALIARMLRDQLVGRMAGR

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=372, Percent_Identity=75, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1790794, Length=356, Percent_Identity=41.5730337078652, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI48994889, Length=229, Percent_Identity=27.9475982532751, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788509, Length=230, Percent_Identity=25.2173913043478, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790146, Length=224, Percent_Identity=23.6607142857143, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40575; Mature: 40575

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPGMLSVVQTFGVDESWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLIGTLYFAA
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TCLAILLTNSIEQFTLMRFLQGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGPLAGAAWVHLFPWEGMFILFAALSLLAFLGLYKAMPETATRRGEKLSLSALGRDYTL
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEHHHCCCCEEE
VLKNRRFLCGSLACGFASLPLLAWIAQSPVIIISGEGLSSYDYGMLQVPIFGMLILGNLT
EEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCHH
LARLSGRRPVRRLIQLGAWPMVGGLAIAAASTLYSAHAYLWMTAGLSLYAFGIGLANAGL
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCH
YRLTLFSSTMSKGTVSAAMGMISMFIYTLGIEVGKYAWLLGGNGAFNLFNLISGLLWLAL
HEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
IARMLRDQLVGRMAGR
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQPGMLSVVQTFGVDESWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLIGTLYFAA
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TCLAILLTNSIEQFTLMRFLQGISLCFIGAVGYAAIQESFEESVCIKITALMANVALIAP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGPLAGAAWVHLFPWEGMFILFAALSLLAFLGLYKAMPETATRRGEKLSLSALGRDYTL
HHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEHHHCCCCEEE
VLKNRRFLCGSLACGFASLPLLAWIAQSPVIIISGEGLSSYDYGMLQVPIFGMLILGNLT
EEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCHH
LARLSGRRPVRRLIQLGAWPMVGGLAIAAASTLYSAHAYLWMTAGLSLYAFGIGLANAGL
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCH
YRLTLFSSTMSKGTVSAAMGMISMFIYTLGIEVGKYAWLLGGNGAFNLFNLISGLLWLAL
HEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
IARMLRDQLVGRMAGR
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]