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The map label for this gene is metF [H]

Identifier: 238921690

GI number: 238921690

Start: 3733375

End: 3734277

Strand: Reverse

Name: metF [H]

Synonym: NT01EI_3851

Alternate gene names: 238921690

Gene position: 3734277-3733375 (Counterclockwise)

Preceding gene: 238921691

Following gene: 238921687

Centisome position: 97.95

GC content: 59.58

Gene sequence:

>903_bases
ATGAGTTTTTTTCACGCAAGCCAGCATGATGCGCTAAACCAGAGCCTGGCCGAGCTGCAGGGGCAGATCCAGGTTTCTTT
TGAGTTTTTTCCGCCGCGCAGCCCGGAGATGGAGCAAACGCTGTGGCGATCCATCGATCGGCTCAGCATCCTGAGGCCGT
CGTTTATTTCGGTGACCTACGGCGCCAACTCCGGCGAGCGCGACCGTACCCACAGCGTGATTAAGGGGATAAAAGAGCGC
ACCGGGCTGGAGGCGGCACCTCACCTGACCTGCGTTGATGCCAGCAGGGAGGCGCTGCGGGGAATAGCCCGGGATTACTG
GAATAGCGGTATCCGCCATATTGTAGCGCTGCGCGGCGATTTGCCGGCGGAAGGCGGGCGTCCGGAAATGTACGCCCGCG
ATCTGGTGGCGCTGTTGCGCGAGGAGGCTGACTTTGATATTTCGGTCGCCGCCTATCCCGAGGTTCACCCGGAGGCTAAG
AGCGCCCAGGCCGACCTGTTAAACCTAAAGCGTAAAATTGACGCCGGTGCCAGCCGGGCGATCACCCAGTTCTTCTTTGA
CGTGGAGAGCTATCTTCGCTTTCGCGATCGCTGTGCGGCAGCCGGTATTGACGCGGAGATTGTTCCGGGGATCTTGCCGG
TGTCCAACTTTCGCCAGCTGCAGCGCTTTGCCGCGCTGACCAATGTCCGCGTGCCGCAGTGGATGCTGGCTATGTTTGAC
GGGTTGGATGAGGACGCGGAGACGCGTAAGCTGGTCGGTGCCAATATTGCCATGGATATGGTGAAACTACTCACCCGCGA
GGGGGTGAAAGACTTTCACTTTTATACCCTGAATCGAGCCGAGCTGAGCTATGCCATTTGCCATACGCTGGGCGTACGGC
CACAGGGTGGCGTGGCGTCGTGA

Upstream 100 bases:

>100_bases
GAATCCATCAACTTACGGCAATTTCTATTTAGACGTCTAAACGTATGGATGTTTAAAGTGGGGGCGCAGAGAGTCGCGCA
GAGCATAAGGATGATGGGGT

Downstream 100 bases:

>100_bases
TAGGCAAAAAAAGCCCGCGGCCGCGGGCTTTTTCCTTTGCATCGCGGCTAGCCGGTATTGCGCATCCCGGCGGCGACTCC
GGCGATAGTGACCATCAGCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSFFHASQHDALNQSLAELQGQIQVSFEFFPPRSPEMEQTLWRSIDRLSILRPSFISVTYGANSGERDRTHSVIKGIKER
TGLEAAPHLTCVDASREALRGIARDYWNSGIRHIVALRGDLPAEGGRPEMYARDLVALLREEADFDISVAAYPEVHPEAK
SAQADLLNLKRKIDAGASRAITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVSNFRQLQRFAALTNVRVPQWMLAMFD
GLDEDAETRKLVGANIAMDMVKLLTREGVKDFHFYTLNRAELSYAICHTLGVRPQGGVAS

Sequences:

>Translated_300_residues
MSFFHASQHDALNQSLAELQGQIQVSFEFFPPRSPEMEQTLWRSIDRLSILRPSFISVTYGANSGERDRTHSVIKGIKER
TGLEAAPHLTCVDASREALRGIARDYWNSGIRHIVALRGDLPAEGGRPEMYARDLVALLREEADFDISVAAYPEVHPEAK
SAQADLLNLKRKIDAGASRAITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVSNFRQLQRFAALTNVRVPQWMLAMFD
GLDEDAETRKLVGANIAMDMVKLLTREGVKDFHFYTLNRAELSYAICHTLGVRPQGGVAS
>Mature_299_residues
SFFHASQHDALNQSLAELQGQIQVSFEFFPPRSPEMEQTLWRSIDRLSILRPSFISVTYGANSGERDRTHSVIKGIKERT
GLEAAPHLTCVDASREALRGIARDYWNSGIRHIVALRGDLPAEGGRPEMYARDLVALLREEADFDISVAAYPEVHPEAKS
AQADLLNLKRKIDAGASRAITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVSNFRQLQRFAALTNVRVPQWMLAMFDG
LDEDAETRKLVGANIAMDMVKLLTREGVKDFHFYTLNRAELSYAICHTLGVRPQGGVAS

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=284, Percent_Identity=35.9154929577465, Blast_Score=154, Evalue=7e-38,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=84.0136054421769, Blast_Score=533, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI25147436, Length=279, Percent_Identity=31.8996415770609, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25147441, Length=279, Percent_Identity=31.8996415770609, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321313, Length=292, Percent_Identity=31.1643835616438, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=31.6326530612245, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24662658, Length=225, Percent_Identity=27.1111111111111, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33539; Mature: 33407

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFHASQHDALNQSLAELQGQIQVSFEFFPPRSPEMEQTLWRSIDRLSILRPSFISVTY
CCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSVIKGIKERTGLEAAPHLTCVDASREALRGIARDYWNSGIRHIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEEHHHCCC
LPAEGGRPEMYARDLVALLREEADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGASRA
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVSNFRQLQRFAALTNVRVPQWMLAMFD
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHC
GLDEDAETRKLVGANIAMDMVKLLTREGVKDFHFYTLNRAELSYAICHTLGVRPQGGVAS
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SFFHASQHDALNQSLAELQGQIQVSFEFFPPRSPEMEQTLWRSIDRLSILRPSFISVTY
CCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSVIKGIKERTGLEAAPHLTCVDASREALRGIARDYWNSGIRHIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHEEHHHCCC
LPAEGGRPEMYARDLVALLREEADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGASRA
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHH
ITQFFFDVESYLRFRDRCAAAGIDAEIVPGILPVSNFRQLQRFAALTNVRVPQWMLAMFD
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHC
GLDEDAETRKLVGANIAMDMVKLLTREGVKDFHFYTLNRAELSYAICHTLGVRPQGGVAS
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEECHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]