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The map label for this gene is argG

Identifier: 238921684

GI number: 238921684

Start: 3726022

End: 3727239

Strand: Reverse

Name: argG

Synonym: NT01EI_3845

Alternate gene names: 238921684

Gene position: 3727239-3726022 (Counterclockwise)

Preceding gene: 238921685

Following gene: 238921683

Centisome position: 97.77

GC content: 60.26

Gene sequence:

>1218_bases
ATGGCAACCCAAAGCATCAGCAAAATCGTTCTGGCCTACTCCGGCGGCCTGGATACCTCTGCCATTATTCCGTGGCTTAA
GGAAAACTACGGCGACTGTGAGGTTATCGCCTTTGTGGCGGACATCGGTCAGGAGCGTGAAGATCTGGTGGGTATCGAGC
AGAAAGCGCTCCGCTCCGGCGCCGCCTCCTGCTATGTCGTGGATCTGCGCGAAACCTTTATCAAGGACTACGTCTATCCG
GTGCTGAAGAGCGGCGCGCTCTATGAGGGTAGCTATCTGCTGGGGACGTCGATGGCCCGTCCACTGATCGCGAAAGCGCA
GGTTGAGCTGGCGCTGGAGCTGGGGGCCGACGCCCTGTGTCACGGCGCGACCGGGAAAGGCAACGATCAGGTGCGTTTTG
AAACCACCTATACTGCGCTGGCGCCGCAGCTGCAGGTCGTGGCACCGTGGCGTGAGTGGGATCTTCGCTCGCGTGAGGCG
CTGCTCGATTATCTGAAGGGGCGCGATATTCCCACTACCGCCACGCTGGAGAAGATCTACAGCCGTGACGCCAACGCTTG
GCATATCTCTACCGAGGGGGGCGTGCTGGAGAGTCCGTGGAGTCCGGCCAACCAAGACTGCTGGGTGTGGACCTGTGCCC
CAGAGGAGGCACCGGATCAGCCTGAACTGGTGACCGTGGGCGTTGAAAAGGGCGAGGTCGTTTCGGTCAATGGGGTGGCG
ATGACCCCCTTTGGCTGCCTGCAGGCGCTGAATGCGCTGGGCACCCGCCACGGCGTAGGACGGATTGATATCGTCGAAAA
CCGTCTGGTGGGCATTAAATCACGTGGCTGCTATGAGACGCCGGGGGGCACCATCATGATGGCAGCGCTGCGCGGCGTGG
AACAACTGGTACTGGATCGCGACAGCTTCCAGTGGCGTGAGAAGCTGGGCCAGGAGATGTCCTATGTCGTGTACGACGGC
CGCTGGTTCGCACCGCTGCGTCAGTCGCTGCAGGCGGCGGCCGATGCGTTGGCTGACGCGGTGAGCGGTGAGGTGGTGAT
TAAGCTGTATAAAGGACAGGCTATCGCCATACAGAAGAAGTCGGTCAACAGCCTGTATAACGAGGCTTTCGCCACCTTTG
GTGAGGATGATGTGTATGATCACAGCCATGCCGGCGGCTTTATTCGCCTGTTCTCCCTCTCTTCACGCATTCGCGCGCTG
AACAGCGCAAAGCAGTAG

Upstream 100 bases:

>100_bases
ACGCCGAAAAGCTGCCGGCGCTGTTTAACGGCGAAGCCGTCGGCACGCGTATTCATGCATAACACAACGACACGATTTGG
TTAAGAATAAGGAAACCCGT

Downstream 100 bases:

>100_bases
TACGCGCAGGGCAGAATGCGGGCCGCGCTGCGGCCGCGGCCCGCCGATCATTTTATTTTGAGTACGGAGCAGTCACTATG
GCACTTTGGGGCGGGCGTTT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MATQSISKIVLAYSGGLDTSAIIPWLKENYGDCEVIAFVADIGQEREDLVGIEQKALRSGAASCYVVDLRETFIKDYVYP
VLKSGALYEGSYLLGTSMARPLIAKAQVELALELGADALCHGATGKGNDQVRFETTYTALAPQLQVVAPWREWDLRSREA
LLDYLKGRDIPTTATLEKIYSRDANAWHISTEGGVLESPWSPANQDCWVWTCAPEEAPDQPELVTVGVEKGEVVSVNGVA
MTPFGCLQALNALGTRHGVGRIDIVENRLVGIKSRGCYETPGGTIMMAALRGVEQLVLDRDSFQWREKLGQEMSYVVYDG
RWFAPLRQSLQAAADALADAVSGEVVIKLYKGQAIAIQKKSVNSLYNEAFATFGEDDVYDHSHAGGFIRLFSLSSRIRAL
NSAKQ

Sequences:

>Translated_405_residues
MATQSISKIVLAYSGGLDTSAIIPWLKENYGDCEVIAFVADIGQEREDLVGIEQKALRSGAASCYVVDLRETFIKDYVYP
VLKSGALYEGSYLLGTSMARPLIAKAQVELALELGADALCHGATGKGNDQVRFETTYTALAPQLQVVAPWREWDLRSREA
LLDYLKGRDIPTTATLEKIYSRDANAWHISTEGGVLESPWSPANQDCWVWTCAPEEAPDQPELVTVGVEKGEVVSVNGVA
MTPFGCLQALNALGTRHGVGRIDIVENRLVGIKSRGCYETPGGTIMMAALRGVEQLVLDRDSFQWREKLGQEMSYVVYDG
RWFAPLRQSLQAAADALADAVSGEVVIKLYKGQAIAIQKKSVNSLYNEAFATFGEDDVYDHSHAGGFIRLFSLSSRIRAL
NSAKQ
>Mature_404_residues
ATQSISKIVLAYSGGLDTSAIIPWLKENYGDCEVIAFVADIGQEREDLVGIEQKALRSGAASCYVVDLRETFIKDYVYPV
LKSGALYEGSYLLGTSMARPLIAKAQVELALELGADALCHGATGKGNDQVRFETTYTALAPQLQVVAPWREWDLRSREAL
LDYLKGRDIPTTATLEKIYSRDANAWHISTEGGVLESPWSPANQDCWVWTCAPEEAPDQPELVTVGVEKGEVVSVNGVAM
TPFGCLQALNALGTRHGVGRIDIVENRLVGIKSRGCYETPGGTIMMAALRGVEQLVLDRDSFQWREKLGQEMSYVVYDGR
WFAPLRQSLQAAADALADAVSGEVVIKLYKGQAIAIQKKSVNSLYNEAFATFGEDDVYDHSHAGGFIRLFSLSSRIRALN
SAKQ

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=41.7085427135678, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=41.7085427135678, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1789563, Length=370, Percent_Identity=29.1891891891892, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=38.6091127098321, Blast_Score=274, Evalue=2e-74,
Organism=Drosophila melanogaster, GI21358151, Length=409, Percent_Identity=41.320293398533, Blast_Score=292, Evalue=2e-79,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_EDWI9 (C5BC58)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935199.1
- GeneID:   7959886
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3845
- OMA:   ADLVTFE
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44298; Mature: 44166

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 40-40 BINDING 92-92 BINDING 97-97 BINDING 122-122 BINDING 124-124 BINDING 128-128 BINDING 128-128 BINDING 129-129 BINDING 132-132 BINDING 181-181 BINDING 190-190 BINDING 266-266 BINDING 278-278

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATQSISKIVLAYSGGLDTSAIIPWLKENYGDCEVIAFVADIGQEREDLVGIEQKALRSG
CCCHHHHHEEEEECCCCCHHHHHHHHHCCCCCHHEEHEEHHHCCCHHHHHHHHHHHHHCC
AASCYVVDLRETFIKDYVYPVLKSGALYEGSYLLGTSMARPLIAKAQVELALELGADALC
CCEEEEEHHHHHHHHHHHHHHHHCCCEECCCEEECHHHHCHHHHHHHHHHHHHCCCCHHC
HGATGKGNDQVRFETTYTALAPQLQVVAPWREWDLRSREALLDYLKGRDIPTTATLEKIY
CCCCCCCCCCEEEEEEHHHHCCCEEEEECCHHCCCCHHHHHHHHHCCCCCCCHHHHHHHH
SRDANAWHISTEGGVLESPWSPANQDCWVWTCAPEEAPDQPELVTVGVEKGEVVSVNGVA
CCCCCEEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEECCEE
MTPFGCLQALNALGTRHGVGRIDIVENRLVGIKSRGCYETPGGTIMMAALRGVEQLVLDR
ECCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHCC
DSFQWREKLGQEMSYVVYDGRWFAPLRQSLQAAADALADAVSGEVVIKLYKGQAIAIQKK
CCHHHHHHHCCCCEEEEECCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEHH
SVNSLYNEAFATFGEDDVYDHSHAGGFIRLFSLSSRIRALNSAKQ
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATQSISKIVLAYSGGLDTSAIIPWLKENYGDCEVIAFVADIGQEREDLVGIEQKALRSG
CCHHHHHEEEEECCCCCHHHHHHHHHCCCCCHHEEHEEHHHCCCHHHHHHHHHHHHHCC
AASCYVVDLRETFIKDYVYPVLKSGALYEGSYLLGTSMARPLIAKAQVELALELGADALC
CCEEEEEHHHHHHHHHHHHHHHHCCCEECCCEEECHHHHCHHHHHHHHHHHHHCCCCHHC
HGATGKGNDQVRFETTYTALAPQLQVVAPWREWDLRSREALLDYLKGRDIPTTATLEKIY
CCCCCCCCCCEEEEEEHHHHCCCEEEEECCHHCCCCHHHHHHHHHCCCCCCCHHHHHHHH
SRDANAWHISTEGGVLESPWSPANQDCWVWTCAPEEAPDQPELVTVGVEKGEVVSVNGVA
CCCCCEEEEECCCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEECCEE
MTPFGCLQALNALGTRHGVGRIDIVENRLVGIKSRGCYETPGGTIMMAALRGVEQLVLDR
ECCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHCC
DSFQWREKLGQEMSYVVYDGRWFAPLRQSLQAAADALADAVSGEVVIKLYKGQAIAIQKK
CCHHHHHHHCCCCEEEEECCEECHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEEHH
SVNSLYNEAFATFGEDDVYDHSHAGGFIRLFSLSSRIRALNSAKQ
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA