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The map label for this gene is trmA

Identifier: 238921674

GI number: 238921674

Start: 3716032

End: 3717132

Strand: Direct

Name: trmA

Synonym: NT01EI_3835

Alternate gene names: 238921674

Gene position: 3716032-3717132 (Clockwise)

Preceding gene: 238921673

Following gene: 238921678

Centisome position: 97.47

GC content: 57.04

Gene sequence:

>1101_bases
ATGACGCCAAGCATGCTGCCAACCGAACACTATGACGACCAGCTCAGTGAAAAAATCCACCGTCTGGAGCAAATGATGCA
GCCGTTCGGTGCCCCTGCGGTACAGCCATTCCGCTCGCCGGTGAGCCATTACCGCATGCGTGCCGAGTTCCGGATCTGGC
ACGATGGCGATGACCTCTACCATATTATGTTTGATCCCCAGAGCAAACAGCGCATCCGGGTGGATCAGTTCCCGGCCGCC
AGCGAACTGATCAACCGCCTGATGCCGCTGCTGCTCGACGCTCTGCGTCCCAACCCAACCCTACGCCATAAACTGTTTCA
GATAGACTACCTGTCCACCCGCAGCGGGCAGATTATCGTATCCCTACTGTATCACCGCACGCTGGATGATGCCTGGAAGC
AGGCCGCAGAGGCTCTGCGCGATGCGTTACGCGGCCAAGGATTCGCTATCCAGCTAATCGGACGGGCCAGTAAGACCAAG
ATCTGCCTAGACCAGGATTGGGTCGATGAGGTATTGCCGGTCGCGGGACGCCAGATGATCTATCGCCAGGTCGAAAACAG
CTTCACCCAGCCCAACGCAGCCATCAATATTCAGATGCTGGAGTGGGCGCTGGACGCCACGCGCGGTGCAGAGGGGGATT
TACTGGAGCTGTACTGCGGCAACGGCAACTTCTCACTGGCGCTGGCACACAACTTTCGTCAGGTGCTGGCCACTGAGATA
GCCAAACCGTCCGTCGCGGCTGCGCAGTACAACATTGCCGCCAATCAGATAAACAACGTACAGATCATTCGCATGTCGGC
AGAGGAGTTTACCCAAGCCATGCAGGGCGTCCGGGCGTTTAACCGTCTGCAGGGCATCGATCTGCACAGCTATGAGTGCA
ATACCATCTTCGTCGATCCCCCACGCAGCGGTCTGGATACAGAAACCCTTGGCATGGTACAGGGATACCCGAACATCCTG
TACATCTCCTGCAATCCACTGACGCTATGCGATAACCTTCACACGCTCAACCAGAGTCATCGGATCACGCGCCTGGCGCT
GTTTGATCAATTTCCGTATACCCACCATATGGAGTGCGGGGTACTGCTGGAACGTCGCTGA

Upstream 100 bases:

>100_bases
GATGGCCATCTGCTCGACCGTTGACTGCCCGTCCCGCCGACGCCCCGTGCGGCGGGATGAACATACCTGCCGTAACTTTC
CCGTGCCGATGAGAACCGAT

Downstream 100 bases:

>100_bases
TCCCAAAAAAACGGGCAGCTCTCTGTCTGGCTGCCCGCGGCGGTGTGACTCAACGGTGTCTCTCATCGTCATCATCATCC
CTATCCTGCACGGTGCGGCT

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MTPSMLPTEHYDDQLSEKIHRLEQMMQPFGAPAVQPFRSPVSHYRMRAEFRIWHDGDDLYHIMFDPQSKQRIRVDQFPAA
SELINRLMPLLLDALRPNPTLRHKLFQIDYLSTRSGQIIVSLLYHRTLDDAWKQAAEALRDALRGQGFAIQLIGRASKTK
ICLDQDWVDEVLPVAGRQMIYRQVENSFTQPNAAINIQMLEWALDATRGAEGDLLELYCGNGNFSLALAHNFRQVLATEI
AKPSVAAAQYNIAANQINNVQIIRMSAEEFTQAMQGVRAFNRLQGIDLHSYECNTIFVDPPRSGLDTETLGMVQGYPNIL
YISCNPLTLCDNLHTLNQSHRITRLALFDQFPYTHHMECGVLLERR

Sequences:

>Translated_366_residues
MTPSMLPTEHYDDQLSEKIHRLEQMMQPFGAPAVQPFRSPVSHYRMRAEFRIWHDGDDLYHIMFDPQSKQRIRVDQFPAA
SELINRLMPLLLDALRPNPTLRHKLFQIDYLSTRSGQIIVSLLYHRTLDDAWKQAAEALRDALRGQGFAIQLIGRASKTK
ICLDQDWVDEVLPVAGRQMIYRQVENSFTQPNAAINIQMLEWALDATRGAEGDLLELYCGNGNFSLALAHNFRQVLATEI
AKPSVAAAQYNIAANQINNVQIIRMSAEEFTQAMQGVRAFNRLQGIDLHSYECNTIFVDPPRSGLDTETLGMVQGYPNIL
YISCNPLTLCDNLHTLNQSHRITRLALFDQFPYTHHMECGVLLERR
>Mature_365_residues
TPSMLPTEHYDDQLSEKIHRLEQMMQPFGAPAVQPFRSPVSHYRMRAEFRIWHDGDDLYHIMFDPQSKQRIRVDQFPAAS
ELINRLMPLLLDALRPNPTLRHKLFQIDYLSTRSGQIIVSLLYHRTLDDAWKQAAEALRDALRGQGFAIQLIGRASKTKI
CLDQDWVDEVLPVAGRQMIYRQVENSFTQPNAAINIQMLEWALDATRGAEGDLLELYCGNGNFSLALAHNFRQVLATEIA
KPSVAAAQYNIAANQINNVQIIRMSAEEFTQAMQGVRAFNRLQGIDLHSYECNTIFVDPPRSGLDTETLGMVQGYPNILY
ISCNPLTLCDNLHTLNQSHRITRLALFDQFPYTHHMECGVLLERR

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=164, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI51173878, Length=164, Percent_Identity=28.0487804878049, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790403, Length=363, Percent_Identity=76.5840220385675, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1789148, Length=185, Percent_Identity=33.5135135135135, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787083, Length=184, Percent_Identity=28.2608695652174, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24666579, Length=166, Percent_Identity=27.710843373494, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_EDWI9 (C5BC48)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935189.1
- ProteinModelPortal:   C5BC48
- GeneID:   7959876
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3835
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 41851; Mature: 41720

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 324-324 BINDING 190-190 BINDING 218-218 BINDING 223-223 BINDING 239-239 BINDING 299-299

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSMLPTEHYDDQLSEKIHRLEQMMQPFGAPAVQPFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEECCCCEE
HIMFDPQSKQRIRVDQFPAASELINRLMPLLLDALRPNPTLRHKLFQIDYLSTRSGQIIV
EEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCCHHHH
SLLYHRTLDDAWKQAAEALRDALRGQGFAIQLIGRASKTKICLDQDWVDEVLPVAGRQMI
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHHHH
YRQVENSFTQPNAAINIQMLEWALDATRGAEGDLLELYCGNGNFSLALAHNFRQVLATEI
HHHHHHHCCCCCCEEEEEEEEEHHHHCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHH
AKPSVAAAQYNIAANQINNVQIIRMSAEEFTQAMQGVRAFNRLQGIDLHSYECNTIFVDP
CCCCHHHHEEEEHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCEECEECCEEEECC
PRSGLDTETLGMVQGYPNILYISCNPLTLCDNLHTLNQSHRITRLALFDQFPYTHHMECG
CCCCCCHHHHHHHCCCCCEEEEECCCEEECCCHHHCCCCCCEEEEEEHHCCCCCCCCCCC
VLLERR
EEEECC
>Mature Secondary Structure 
TPSMLPTEHYDDQLSEKIHRLEQMMQPFGAPAVQPFRSPVSHYRMRAEFRIWHDGDDLY
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEEEEECCCCEE
HIMFDPQSKQRIRVDQFPAASELINRLMPLLLDALRPNPTLRHKLFQIDYLSTRSGQIIV
EEEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCCHHHH
SLLYHRTLDDAWKQAAEALRDALRGQGFAIQLIGRASKTKICLDQDWVDEVLPVAGRQMI
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECHHHHHHHHHHHHHHHH
YRQVENSFTQPNAAINIQMLEWALDATRGAEGDLLELYCGNGNFSLALAHNFRQVLATEI
HHHHHHHCCCCCCEEEEEEEEEHHHHCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHHH
AKPSVAAAQYNIAANQINNVQIIRMSAEEFTQAMQGVRAFNRLQGIDLHSYECNTIFVDP
CCCCHHHHEEEEHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCEECEECCEEEECC
PRSGLDTETLGMVQGYPNILYISCNPLTLCDNLHTLNQSHRITRLALFDQFPYTHHMECG
CCCCCCHHHHHHHCCCCCEEEEECCCEEECCCHHHCCCCCCEEEEEEHHCCCCCCCCCCC
VLLERR
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA