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The map label for this gene is pfkA

Identifier: 238921652

GI number: 238921652

Start: 3689310

End: 3690272

Strand: Reverse

Name: pfkA

Synonym: NT01EI_3808

Alternate gene names: 238921652

Gene position: 3690272-3689310 (Counterclockwise)

Preceding gene: 238921653

Following gene: 238921649

Centisome position: 96.8

GC content: 56.07

Gene sequence:

>963_bases
ATGATCAAAAAAATCGGTGTTTTGACCAGTGGCGGTGATGCGCCGGGCATGAACGCAGCCATCCGCGGCGTTGTGCGTGC
GGCCCTGTCCAAAGGGTTAGATGTTTACGGCATTCATGATGGCTACCTCGGTCTGTATGAAGGACGCATTGAGCAACTGG
ATCGCCATAGTGTTTCCGACGTGATCAACCGCGGCGGCACTTTCCTGGGCTCAGCCCGTTTCCCGGAGTTTCGCGACGAG
AAAGTTCGTGAAAAGGCCGTAGAGAATATGCGTGAGTTTGGCCTGGATGCGCTGGTGGTGATCGGCGGCGACGGTTCTTA
TCTGGGCGCCAAGCGCCTGACCGAGATGGGATTCCCCTGCATTGGCCTACCGGGGACTATCGATAACGATGTGGCCGGCA
CCGATTACACTATCGGGTATTTTACGGCGCTGGAAACCGTTGTGGAGGCCATTGATCGTCTGCGTGATACCTCCTCTTCA
CACCAGCGTATCTCCATCGTTGAGGTGATGGGGCGCTACTGCGGCGATCTGACGCTGGCGGCGGCGATTGCCGGTGGCTG
CGAATATATCGTGCTGCCGGAGGTGGAGTTCAAGCGCGAAGATCTGGTCGAAGAGATTAAGGTCGGTATCACTAAAGGCA
AGAAACACGCTATCGTTGCTATCACCGAGCATATCTGTGACGTGGATGAGCTGGCCAAGTTTATCGAACAGGAGACCGGC
CGTGAGACTCGTTCGACCGTTCTGGGACATATCCAGCGCGGTGGTGCGCCGGTGGCCTATGACCGTATTCTGGCGTCCCG
TATGGGAGCCTACTCCATCGAGCTGCTGCTGCAGGGATACGGCGGTCGCTGCGTGGGGATCCAGAACGAAAAGCTGGTAC
ATCACGACATCATCGATGCGGTTGAAAATATGAAGCGTCCATTTAAGGGCGATTGGCTGGATACTGCCAAGATGCTGTTC
TAA

Upstream 100 bases:

>100_bases
TGGGTGTCACGTCGACCGTCGCCGTTTCCGACGGCCGGGGCTTGGGTTGTACGCGCCAAGCCATTCCTAAGAAATAGTAT
CCACAAGCTCAGAGGTAGTC

Downstream 100 bases:

>100_bases
TGGCGCTTGTGCTACCGTAACTATCAGAATATAAAGAAAGTCTGATAGATAAGGACATCAAGGGAAAAGTTGGCTTCAGG
ATATTTTAGCAGAGGCTAAT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MIKKIGVLTSGGDAPGMNAAIRGVVRAALSKGLDVYGIHDGYLGLYEGRIEQLDRHSVSDVINRGGTFLGSARFPEFRDE
KVREKAVENMREFGLDALVVIGGDGSYLGAKRLTEMGFPCIGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEYIVLPEVEFKREDLVEEIKVGITKGKKHAIVAITEHICDVDELAKFIEQETG
RETRSTVLGHIQRGGAPVAYDRILASRMGAYSIELLLQGYGGRCVGIQNEKLVHHDIIDAVENMKRPFKGDWLDTAKMLF

Sequences:

>Translated_320_residues
MIKKIGVLTSGGDAPGMNAAIRGVVRAALSKGLDVYGIHDGYLGLYEGRIEQLDRHSVSDVINRGGTFLGSARFPEFRDE
KVREKAVENMREFGLDALVVIGGDGSYLGAKRLTEMGFPCIGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEYIVLPEVEFKREDLVEEIKVGITKGKKHAIVAITEHICDVDELAKFIEQETG
RETRSTVLGHIQRGGAPVAYDRILASRMGAYSIELLLQGYGGRCVGIQNEKLVHHDIIDAVENMKRPFKGDWLDTAKMLF
>Mature_320_residues
MIKKIGVLTSGGDAPGMNAAIRGVVRAALSKGLDVYGIHDGYLGLYEGRIEQLDRHSVSDVINRGGTFLGSARFPEFRDE
KVREKAVENMREFGLDALVVIGGDGSYLGAKRLTEMGFPCIGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSS
HQRISIVEVMGRYCGDLTLAAAIAGGCEYIVLPEVEFKREDLVEEIKVGITKGKKHAIVAITEHICDVDELAKFIEQETG
RETRSTVLGHIQRGGAPVAYDRILASRMGAYSIELLLQGYGGRCVGIQNEKLVHHDIIDAVENMKRPFKGDWLDTAKMLF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=312, Percent_Identity=40.0641025641026, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI266453768, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI266453748, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI4505749, Length=312, Percent_Identity=39.1025641025641, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI266453619, Length=312, Percent_Identity=39.1025641025641, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI48762920, Length=313, Percent_Identity=40.5750798722045, Blast_Score=202, Evalue=4e-52,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=84.6875, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI25147584, Length=359, Percent_Identity=36.4902506963788, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17558788, Length=308, Percent_Identity=38.6363636363636, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=30.9278350515464, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321679, Length=342, Percent_Identity=37.4269005847953, Blast_Score=205, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6323861, Length=344, Percent_Identity=35.1744186046512, Blast_Score=186, Evalue=6e-48,
Organism=Drosophila melanogaster, GI28573326, Length=359, Percent_Identity=37.6044568245125, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24652337, Length=358, Percent_Identity=36.8715083798883, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI17647809, Length=312, Percent_Identity=40.0641025641026, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_EDWI9 (C5BC26)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002935167.1
- ProteinModelPortal:   C5BC26
- GeneID:   7959316
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3808
- OMA:   VDAIENM
- ProtClustDB:   CLSK2813739
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34954; Mature: 34954

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 128-128 BINDING 163-163 BINDING 244-244 BINDING 250-250 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKIGVLTSGGDAPGMNAAIRGVVRAALSKGLDVYGIHDGYLGLYEGRIEQLDRHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
VINRGGTFLGSARFPEFRDEKVREKAVENMREFGLDALVVIGGDGSYLGAKRLTEMGFPC
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
IGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAIAGGCEYIVLPEVEFKREDLVEEIKVGITKGKKHAIVAITEHICDVDELAKFIEQETG
HHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHHHCC
RETRSTVLGHIQRGGAPVAYDRILASRMGAYSIELLLQGYGGRCVGIQNEKLVHHDIIDA
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEHHEECCCCEEEECCCCCHHHHHHHHH
VENMKRPFKGDWLDTAKMLF
HHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MIKKIGVLTSGGDAPGMNAAIRGVVRAALSKGLDVYGIHDGYLGLYEGRIEQLDRHSVSD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
VINRGGTFLGSARFPEFRDEKVREKAVENMREFGLDALVVIGGDGSYLGAKRLTEMGFPC
HHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCCC
IGLPGTIDNDVAGTDYTIGYFTALETVVEAIDRLRDTSSSHQRISIVEVMGRYCGDLTLA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAIAGGCEYIVLPEVEFKREDLVEEIKVGITKGKKHAIVAITEHICDVDELAKFIEQETG
HHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHHHHHHCC
RETRSTVLGHIQRGGAPVAYDRILASRMGAYSIELLLQGYGGRCVGIQNEKLVHHDIIDA
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEHHEECCCCEEEECCCCCHHHHHHHHH
VENMKRPFKGDWLDTAKMLF
HHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA