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The map label for this gene is metB [H]

Identifier: 238921650

GI number: 238921650

Start: 3685428

End: 3686588

Strand: Direct

Name: metB [H]

Synonym: NT01EI_3805

Alternate gene names: 238921650

Gene position: 3685428-3686588 (Clockwise)

Preceding gene: 238921648

Following gene: 238921651

Centisome position: 96.67

GC content: 63.91

Gene sequence:

>1161_bases
GTGCGCTATCAACCGGCAACTCAGGCAGTACGCTGTGGGCTGAACGACGATGAACAGTATGGCAGCGTGGTTCCCCCAAT
CCATCTTTCCACCACCTACAACTTTACCGATTTTAATCAGCCACGCACGCATGACTATACCCGGCGCGGGAATCCCACAC
GAGACGTGGCCGAGCGGGCGCTGGCGGAGCTGGAGGGGGGCGCCGGTGCGGTGGTGACCGCCAGCGGGATGGGTGCCATT
CATCTGATGTGTACGGCGCTGCTCTCCCCCGGCGATCTGCTGCTGGCGCCGCACGACTGCTATGGCGGCAGCTATCGCCT
GTTCGATAGCTTGGCGCGCCGGGGCGCTTTCCGGGTGGTATTTGTCGATCAGGGCGATGCGCAGGCGCTGGCCCAGGCGC
TGGCGCTGCGCCCAAAGCTGGTGCTGATTGAGACGCCGAGCAATCCGCTGCTGCGGGTAGTGGATATCGCCGCGATCTGC
GCCGCCGCGCAGCGGATGGGTGCCCTGACGGCGGTAGATAATACGTTCCTCAGTCCGGCACTGCAGCAGCCGCTGGCACT
GGGCGCCGATCTGGTGCTGCACTCCTGTACCAAGTACCTGAACGGCCACTCCGACGTGGTGGCCGGGGCGGTGATCGCCA
AAACGGCGGCGATGGCGCAGGAGCTGGCCTGGTGGGGAAATAATATCGGGGTGACCGGCGCACCTTTTGACTGCTATCTG
CTGCTGCGGGGGCTGCGCACGCTGGCGCCGCGTATCGCGCAGCAGCAGGTCAATGCGCTGCGCATCCTCGACTGGCTGCG
GACGCAGCCGCAGGTCGCGCGGCTGTATCATCCGTCGCTGCCCACGCACCCCGGCCATGCCATTGCCGCCCGCCAGCAGC
GGGGCTTTGGCGCCATGCTCAGTTTTGAGCTAGAGGGAGATGAGTCCCATCTACGGCGTTTCCTAAAATCCCTGCAACTG
TTTACACTGGCAGAGTCTCTGGGGGGGGTGGAGAGCCTGATCTCCCATGCCGCCACCATGACCCACGCCGGCATGGCACC
GGAAGCGCGCAGGGCAGCAGGCATTAGCGACAGCCTGCTGCGTATTTCCGTGGGTATTGAGGACGGTGATGATTTAATCG
CTGATCTGGAACAGGCATTTCAGGCAGCAGCAACGAGGTAA

Upstream 100 bases:

>100_bases
CTACACGCAAAGACTTTTAGATGGCTGGATGTATTGACGTCCATTTTTGCAGCGGTTACGCTCTATCCATTGTTGGCACC
ATTTCAGGGAGGGCAATCAG

Downstream 100 bases:

>100_bases
GCGATGAGTGCACAAGCGGTAACGGGGCAGCAGCAGGCAGATCGCCAACTGCATAAGTTCGGCGGCAGTAGCCTGGCAGA
CGTAAAGTGTTATTTGCGCG

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MRYQPATQAVRCGLNDDEQYGSVVPPIHLSTTYNFTDFNQPRTHDYTRRGNPTRDVAERALAELEGGAGAVVTASGMGAI
HLMCTALLSPGDLLLAPHDCYGGSYRLFDSLARRGAFRVVFVDQGDAQALAQALALRPKLVLIETPSNPLLRVVDIAAIC
AAAQRMGALTAVDNTFLSPALQQPLALGADLVLHSCTKYLNGHSDVVAGAVIAKTAAMAQELAWWGNNIGVTGAPFDCYL
LLRGLRTLAPRIAQQQVNALRILDWLRTQPQVARLYHPSLPTHPGHAIAARQQRGFGAMLSFELEGDESHLRRFLKSLQL
FTLAESLGGVESLISHAATMTHAGMAPEARRAAGISDSLLRISVGIEDGDDLIADLEQAFQAAATR

Sequences:

>Translated_386_residues
MRYQPATQAVRCGLNDDEQYGSVVPPIHLSTTYNFTDFNQPRTHDYTRRGNPTRDVAERALAELEGGAGAVVTASGMGAI
HLMCTALLSPGDLLLAPHDCYGGSYRLFDSLARRGAFRVVFVDQGDAQALAQALALRPKLVLIETPSNPLLRVVDIAAIC
AAAQRMGALTAVDNTFLSPALQQPLALGADLVLHSCTKYLNGHSDVVAGAVIAKTAAMAQELAWWGNNIGVTGAPFDCYL
LLRGLRTLAPRIAQQQVNALRILDWLRTQPQVARLYHPSLPTHPGHAIAARQQRGFGAMLSFELEGDESHLRRFLKSLQL
FTLAESLGGVESLISHAATMTHAGMAPEARRAAGISDSLLRISVGIEDGDDLIADLEQAFQAAATR
>Mature_386_residues
MRYQPATQAVRCGLNDDEQYGSVVPPIHLSTTYNFTDFNQPRTHDYTRRGNPTRDVAERALAELEGGAGAVVTASGMGAI
HLMCTALLSPGDLLLAPHDCYGGSYRLFDSLARRGAFRVVFVDQGDAQALAQALALRPKLVLIETPSNPLLRVVDIAAIC
AAAQRMGALTAVDNTFLSPALQQPLALGADLVLHSCTKYLNGHSDVVAGAVIAKTAAMAQELAWWGNNIGVTGAPFDCYL
LLRGLRTLAPRIAQQQVNALRILDWLRTQPQVARLYHPSLPTHPGHAIAARQQRGFGAMLSFELEGDESHLRRFLKSLQL
FTLAESLGGVESLISHAATMTHAGMAPEARRAAGISDSLLRISVGIEDGDDLIADLEQAFQAAATR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=381, Percent_Identity=43.5695538057743, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI299473759, Length=381, Percent_Identity=40.6824146981627, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI25453487, Length=380, Percent_Identity=39.4736842105263, Blast_Score=234, Evalue=1e-61,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=76.5013054830287, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=30.9462915601023, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=385, Percent_Identity=44.4155844155844, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17560056, Length=382, Percent_Identity=44.5026178010471, Blast_Score=296, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=35.5013550135501, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=40.1554404145078, Blast_Score=268, Evalue=9e-73,
Organism=Saccharomyces cerevisiae, GI6321254, Length=396, Percent_Identity=34.5959595959596, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321170, Length=333, Percent_Identity=30.03003003003, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323333, Length=409, Percent_Identity=28.6063569682152, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=386, Percent_Identity=30.0518134715026, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922554, Length=389, Percent_Identity=43.4447300771208, Blast_Score=328, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41464; Mature: 41464

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYQPATQAVRCGLNDDEQYGSVVPPIHLSTTYNFTDFNQPRTHDYTRRGNPTRDVAERA
CCCCCCCHHHHCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVVTASGMGAIHLMCTALLSPGDLLLAPHDCYGGSYRLFDSLARRGAFRVV
HHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGDAQALAQALALRPKLVLIETPSNPLLRVVDIAAICAAAQRMGALTAVDNTFLSPA
EECCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHH
LQQPLALGADLVLHSCTKYLNGHSDVVAGAVIAKTAAMAQELAWWGNNIGVTGAPFDCYL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LLRGLRTLAPRIAQQQVNALRILDWLRTQPQVARLYHPSLPTHPGHAIAARQQRGFGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEE
SFELEGDESHLRRFLKSLQLFTLAESLGGVESLISHAATMTHAGMAPEARRAAGISDSLL
EEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEE
RISVGIEDGDDLIADLEQAFQAAATR
EEEECCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRYQPATQAVRCGLNDDEQYGSVVPPIHLSTTYNFTDFNQPRTHDYTRRGNPTRDVAERA
CCCCCCCHHHHCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVVTASGMGAIHLMCTALLSPGDLLLAPHDCYGGSYRLFDSLARRGAFRVV
HHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGDAQALAQALALRPKLVLIETPSNPLLRVVDIAAICAAAQRMGALTAVDNTFLSPA
EECCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCHH
LQQPLALGADLVLHSCTKYLNGHSDVVAGAVIAKTAAMAQELAWWGNNIGVTGAPFDCYL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LLRGLRTLAPRIAQQQVNALRILDWLRTQPQVARLYHPSLPTHPGHAIAARQQRGFGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHCCCCCEEE
SFELEGDESHLRRFLKSLQLFTLAESLGGVESLISHAATMTHAGMAPEARRAAGISDSLL
EEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEE
RISVGIEDGDDLIADLEQAFQAAATR
EEEECCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]