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The map label for this gene is fdnI [H]

Identifier: 238921564

GI number: 238921564

Start: 3602542

End: 3603192

Strand: Direct

Name: fdnI [H]

Synonym: NT01EI_3716

Alternate gene names: 238921564

Gene position: 3602542-3603192 (Clockwise)

Preceding gene: 238921563

Following gene: 238921565

Centisome position: 94.5

GC content: 57.14

Gene sequence:

>651_bases
ATGAGCAAAAATAATCTGATCCTGCGCACCCGGTTTATTGACCGGGCGTGTCACTGGACGGTGGTGATCTGCTTCTTCCT
GGTCGCGCTATCCGGGATAGCGCTGTTCTTCCCCACGCTACAGTGGCTGACCGAAACCTTCGGAACACCGCAGATGGGGC
GGATTCTGCACCCCTTCTTCGGCGTGCTGATCTTTGTTACCCTGCTGGTTATGTTCTTCCGCTTCGTAAGGCATAACCTG
TTCGAACGCCAGGATATTCCCTGGTTTAAGGGGATCATTGAGGTGCTGAAGGGCAACGAGCACCTGGTGGCGCGCGTGGG
TAAATATAACCCGGGGCAGAAAGCGATGTTCTGGAGCATCATGAGCCTGATCCTGGTGCTGCTGATCACCGGCATCATCA
TCTGGCGCCCCTACTTCGCCGACCGCTTCCCGATCCCGCTGGTGCGCTACTCGCTGCTGCTGCACGCCGTCGCCGCCATC
GTGCTGATCCATGCCATCCTGATCCATATCTACATGGCATTTTGGGTCAAAGGCTCGATCAAAGGGATGGTGGAAGGCAA
GGTCAGCCGTCGCTGGGCCAGAAAACACCACCCGCGCTGGCTAGAAGGAGTGGAAGAAAAGGAGGCCCAGTCCGGTAAAA
AGCCGGGCTGA

Upstream 100 bases:

>100_bases
CCCAATACGGAGGACGCAGACGATCACGGCGATACGCCGCGGGGTAACGCTGGCGGGCAGGAGCATAGCCAGAAGCAAGG
ACATGCAGAGGAGAATCACC

Downstream 100 bases:

>100_bases
TCCCCCATTCTGTCGCGGACTCCCGTCGTCCCCCGGGCGGCGGGAGTCCGCGATGTATTCACAACACATCAGACGAAACC
CTATGAGCATTCGAATTCAT

Product: formate dehydrogenase-N subunit gamma

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase cytochrome b556 subunit; Formate dehydrogenase-N subunit gamma; FDH-N subunit gamma [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSKNNLILRTRFIDRACHWTVVICFFLVALSGIALFFPTLQWLTETFGTPQMGRILHPFFGVLIFVTLLVMFFRFVRHNL
FERQDIPWFKGIIEVLKGNEHLVARVGKYNPGQKAMFWSIMSLILVLLITGIIIWRPYFADRFPIPLVRYSLLLHAVAAI
VLIHAILIHIYMAFWVKGSIKGMVEGKVSRRWARKHHPRWLEGVEEKEAQSGKKPG

Sequences:

>Translated_216_residues
MSKNNLILRTRFIDRACHWTVVICFFLVALSGIALFFPTLQWLTETFGTPQMGRILHPFFGVLIFVTLLVMFFRFVRHNL
FERQDIPWFKGIIEVLKGNEHLVARVGKYNPGQKAMFWSIMSLILVLLITGIIIWRPYFADRFPIPLVRYSLLLHAVAAI
VLIHAILIHIYMAFWVKGSIKGMVEGKVSRRWARKHHPRWLEGVEEKEAQSGKKPG
>Mature_215_residues
SKNNLILRTRFIDRACHWTVVICFFLVALSGIALFFPTLQWLTETFGTPQMGRILHPFFGVLIFVTLLVMFFRFVRHNLF
ERQDIPWFKGIIEVLKGNEHLVARVGKYNPGQKAMFWSIMSLILVLLITGIIIWRPYFADRFPIPLVRYSLLLHAVAAIV
LIHAILIHIYMAFWVKGSIKGMVEGKVSRRWARKHHPRWLEGVEEKEAQSGKKPG

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556(FDN) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=216, Percent_Identity=74.0740740740741, Blast_Score=327, Evalue=4e-91,
Organism=Escherichia coli, GI1790325, Length=213, Percent_Identity=47.4178403755869, Blast_Score=218, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 25128; Mature: 24996

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNNLILRTRFIDRACHWTVVICFFLVALSGIALFFPTLQWLTETFGTPQMGRILHPFF
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GVLIFVTLLVMFFRFVRHNLFERQDIPWFKGIIEVLKGNEHLVARVGKYNPGQKAMFWSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHH
MSLILVLLITGIIIWRPYFADRFPIPLVRYSLLLHAVAAIVLIHAILIHIYMAFWVKGSI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGMVEGKVSRRWARKHHPRWLEGVEEKEAQSGKKPG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKNNLILRTRFIDRACHWTVVICFFLVALSGIALFFPTLQWLTETFGTPQMGRILHPFF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GVLIFVTLLVMFFRFVRHNLFERQDIPWFKGIIEVLKGNEHLVARVGKYNPGQKAMFWSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHH
MSLILVLLITGIIIWRPYFADRFPIPLVRYSLLLHAVAAIVLIHAILIHIYMAFWVKGSI
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGMVEGKVSRRWARKHHPRWLEGVEEKEAQSGKKPG
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]