Click here to switch to the map view.

The map label for this gene is fdnH [H]

Identifier: 238921563

GI number: 238921563

Start: 3601602

End: 3602555

Strand: Direct

Name: fdnH [H]

Synonym: NT01EI_3715

Alternate gene names: 238921563

Gene position: 3601602-3602555 (Clockwise)

Preceding gene: 238921562

Following gene: 238921564

Centisome position: 94.47

GC content: 62.05

Gene sequence:

>954_bases
ATGTCACAGCAATCCCAAGACATTATTAAGCGTTCGGCCACCAATTCCCTGACGCCGCCCCCCAACGGGCGCAATCACCA
GGAGGAAGTGGCCAAGCTTATCGACGTGACCACCTGCGTCGGCTGTAAGGCCTGTCAGGTGGGCTGTTCAGAGTGGAACG
ATATCCGGGATGAGGTCGGACACTGCGTCGGGGTGTATGACAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATG
CGCTTCTCGGAGGTCGAGGAGAACGGTAAGCTGGAGTGGCTGATCCGTAAGGATGGCTGCATGCACTGTGCCGATCCGGG
CTGCCTGAAGGCCTGTCCGTCGGCAGGCGCCATCATCCAGTACGCCAACGGTATCGTCGACTTTCAGTCCGAGCACTGCA
TCGGCTGCGGCTACTGCATCGCCGGCTGCCCGTTCAATGTGCCGCGCATGAATCCGGAAGACAAACGGGTGTACAAGTGC
ACCCTGTGCGTCGACCGCGTCACCGTCGGCCAAGAGCCAGCCTGCGTCAAAACCTGCCCGACCGGCGCCATCCACTTTGG
CACCAAGGCGGCGATGAAGACGCTGGCGGCCGAGCGCATCAGCGAGCTGCAGGGCCGCGGCTTCGCCGATGCCGGCCTGT
ACGATCCGCCGGGCGTCGGTGGCACCCACGTGATGTATGTGCTGCACCACGCCGACCGGCCCGAGCTGTACCACGGGCTG
CCGAAGGATCCGCATATCGATACCCCCATCACGCTGTGGAAGGATGTGCTCAAACCGGTCGCCTTTGCCGGTTTCATCGC
CACCTTTATCGGGCTGCTGTACCACTACATCGCCGTCGGCCCCAATACGGAGGACGCAGACGATCACGGCGATACGCCGC
GGGGTAACGCTGGCGGGCAGGAGCATAGCCAGAAGCAAGGACATGCAGAGGAGAATCACCATGAGCAAAAATAA

Upstream 100 bases:

>100_bases
GGCCAACACGCTGACGCCGTTTGTCGGCGATGCCAATACGCAGACGCCGGAGTTCAAGGCGTTCCTGGTTAACGTGGAAA
AGGTGTAACGGAGGCTAACC

Downstream 100 bases:

>100_bases
TCTGATCCTGCGCACCCGGTTTATTGACCGGGCGTGTCACTGGACGGTGGTGATCTGCTTCTTCCTGGTCGCGCTATCCG
GGATAGCGCTGTTCTTCCCC

Product: formate dehydrogenase, nitrate-inducible, iron-sulfur subunit

Products: CO2; NADH

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSQQSQDIIKRSATNSLTPPPNGRNHQEEVAKLIDVTTCVGCKACQVGCSEWNDIRDEVGHCVGVYDNPADLSAKSWTVM
RFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSAGAIIQYANGIVDFQSEHCIGCGYCIAGCPFNVPRMNPEDKRVYKC
TLCVDRVTVGQEPACVKTCPTGAIHFGTKAAMKTLAAERISELQGRGFADAGLYDPPGVGGTHVMYVLHHADRPELYHGL
PKDPHIDTPITLWKDVLKPVAFAGFIATFIGLLYHYIAVGPNTEDADDHGDTPRGNAGGQEHSQKQGHAEENHHEQK

Sequences:

>Translated_317_residues
MSQQSQDIIKRSATNSLTPPPNGRNHQEEVAKLIDVTTCVGCKACQVGCSEWNDIRDEVGHCVGVYDNPADLSAKSWTVM
RFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSAGAIIQYANGIVDFQSEHCIGCGYCIAGCPFNVPRMNPEDKRVYKC
TLCVDRVTVGQEPACVKTCPTGAIHFGTKAAMKTLAAERISELQGRGFADAGLYDPPGVGGTHVMYVLHHADRPELYHGL
PKDPHIDTPITLWKDVLKPVAFAGFIATFIGLLYHYIAVGPNTEDADDHGDTPRGNAGGQEHSQKQGHAEENHHEQK
>Mature_316_residues
SQQSQDIIKRSATNSLTPPPNGRNHQEEVAKLIDVTTCVGCKACQVGCSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMR
FSEVEENGKLEWLIRKDGCMHCADPGCLKACPSAGAIIQYANGIVDFQSEHCIGCGYCIAGCPFNVPRMNPEDKRVYKCT
LCVDRVTVGQEPACVKTCPTGAIHFGTKAAMKTLAAERISELQGRGFADAGLYDPPGVGGTHVMYVLHHADRPELYHGLP
KDPHIDTPITLWKDVLKPVAFAGFIATFIGLLYHYIAVGPNTEDADDHGDTPRGNAGGQEHSQKQGHAEENHHEQK

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=291, Percent_Identity=79.3814432989691, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1790326, Length=289, Percent_Identity=76.1245674740484, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789370, Length=279, Percent_Identity=27.9569892473118, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787872, Length=173, Percent_Identity=33.5260115606936, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787122, Length=159, Percent_Identity=33.9622641509434, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI2367345, Length=154, Percent_Identity=32.4675324675325, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI226510944, Length=205, Percent_Identity=29.7560975609756, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787963, Length=148, Percent_Identity=25.6756756756757, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082179, Length=172, Percent_Identity=27.906976744186, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787740, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787478, Length=100, Percent_Identity=34, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 34524; Mature: 34393

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
5.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQSQDIIKRSATNSLTPPPNGRNHQEEVAKLIDVTTCVGCKACQVGCSEWNDIRDEVG
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHC
HCVGVYDNPADLSAKSWTVMRFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSAGAIIQ
CEEECCCCCCCCCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCHHHH
YANGIVDFQSEHCIGCGYCIAGCPFNVPRMNPEDKRVYKCTLCVDRVTVGQEPACVKTCP
HHCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCEEECCC
TGAIHFGTKAAMKTLAAERISELQGRGFADAGLYDPPGVGGTHVMYVLHHADRPELYHGL
CCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCC
PKDPHIDTPITLWKDVLKPVAFAGFIATFIGLLYHYIAVGPNTEDADDHGDTPRGNAGGQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
EHSQKQGHAEENHHEQK
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SQQSQDIIKRSATNSLTPPPNGRNHQEEVAKLIDVTTCVGCKACQVGCSEWNDIRDEVG
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHC
HCVGVYDNPADLSAKSWTVMRFSEVEENGKLEWLIRKDGCMHCADPGCLKACPSAGAIIQ
CEEECCCCCCCCCCCCEEEEEHHHHCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCHHHH
YANGIVDFQSEHCIGCGYCIAGCPFNVPRMNPEDKRVYKCTLCVDRVTVGQEPACVKTCP
HHCCEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEEHHHHHCCCCCCEEECCC
TGAIHFGTKAAMKTLAAERISELQGRGFADAGLYDPPGVGGTHVMYVLHHADRPELYHGL
CCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCC
PKDPHIDTPITLWKDVLKPVAFAGFIATFIGLLYHYIAVGPNTEDADDHGDTPRGNAGGQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
EHSQKQGHAEENHHEQK
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]