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The map label for this gene is sgaE [H]

Identifier: 238921511

GI number: 238921511

Start: 3548170

End: 3548859

Strand: Direct

Name: sgaE [H]

Synonym: NT01EI_3663

Alternate gene names: 238921511

Gene position: 3548170-3548859 (Clockwise)

Preceding gene: 238921510

Following gene: 238921516

Centisome position: 93.07

GC content: 66.09

Gene sequence:

>690_bases
ATGGAACGGCTGAAACAGCAGGTCTGCCAGGCCAACATGGCCCTGCCGCACCACGGGCTGGTCACCTTTACCTGGGGCAA
CGTCAGCGCCATCGATCGCCAGTGCGGCCATGTCGTCATCAAACCCAGCGGCGTCGCCTATGAGGCACTGACCCCCGATG
AGATGGTCGTCGTCGATCTTGACGGACGGATCGTCGCTGGCGCGCTGCGCCCCTCCTCCGACACCCCGACCCACCTGGCA
CTGTACCGTCGCTACCCGCAGCTGGGCGGCATTGTGCATACCCACTCGACCCATGCGACCGCCTGGGCACAGGCGGGGCG
GGCCATCCCGGCGCTCGGCACCACCCACGCTGACTACTTCGATGGCGACATTCCCTGCACCCGCGCGCTGAGCACCGCCG
AGATCGACCACGACTATGAGCATCACACCGGGCTGGTCATCATCGAAACGCTGGCGCAGCGCGACCCGGGCCATATGCCG
GGGATCGTCGTTCACCAGCACGGCTCCTTCGCGTGGGGAGAGAGCGCGGCACAGGCGGTGCATAACGCGGTGGTGCTGGA
AGAGGTGGCCAAAATGGCCTGGATTGCCCATAGCCTGAACCCGCGGCTCGCCCCGATGGATCGGGCGCTGCACGATAAAC
ATTTCCGGCGCAAGCACGGACCGGATGCCTACTACGGCCAGCGCGGCTAA

Upstream 100 bases:

>100_bases
ACGAAAGCGCCCCGGGCCCTCTGCAGGCGGTGCGCGAGGCGCGCGACTGGGTCATGGAGCGGATGCGGCGTGCCGGGCTG
GCGGTGGCACAGGAGGCATC

Downstream 100 bases:

>100_bases
CGCGTCGGCTCCCCGGCTCAGTCGCGCAGTCGGCGGCTGAGCCACAGCCCGTGATGCGGGCGCTGGCGCCAGGCGATAGA
TGAAATGGTATGCATGGTAC

Product: L-ribulose-5-phosphate 4-epimerase

Products: NA

Alternate protein names: L-ascorbate utilization protein F; Phosphoribulose isomerase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MERLKQQVCQANMALPHHGLVTFTWGNVSAIDRQCGHVVIKPSGVAYEALTPDEMVVVDLDGRIVAGALRPSSDTPTHLA
LYRRYPQLGGIVHTHSTHATAWAQAGRAIPALGTTHADYFDGDIPCTRALSTAEIDHDYEHHTGLVIIETLAQRDPGHMP
GIVVHQHGSFAWGESAAQAVHNAVVLEEVAKMAWIAHSLNPRLAPMDRALHDKHFRRKHGPDAYYGQRG

Sequences:

>Translated_229_residues
MERLKQQVCQANMALPHHGLVTFTWGNVSAIDRQCGHVVIKPSGVAYEALTPDEMVVVDLDGRIVAGALRPSSDTPTHLA
LYRRYPQLGGIVHTHSTHATAWAQAGRAIPALGTTHADYFDGDIPCTRALSTAEIDHDYEHHTGLVIIETLAQRDPGHMP
GIVVHQHGSFAWGESAAQAVHNAVVLEEVAKMAWIAHSLNPRLAPMDRALHDKHFRRKHGPDAYYGQRG
>Mature_229_residues
MERLKQQVCQANMALPHHGLVTFTWGNVSAIDRQCGHVVIKPSGVAYEALTPDEMVVVDLDGRIVAGALRPSSDTPTHLA
LYRRYPQLGGIVHTHSTHATAWAQAGRAIPALGTTHADYFDGDIPCTRALSTAEIDHDYEHHTGLVIIETLAQRDPGHMP
GIVVHQHGSFAWGESAAQAVHNAVVLEEVAKMAWIAHSLNPRLAPMDRALHDKHFRRKHGPDAYYGQRG

Specific function: Catalyzes the isomerization of L-ribulose 5-phosphate to D-xylulose 5-phosphate. Is involved in the anaerobic L-ascorbate utilization [H]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family. AraD/FucA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790642, Length=228, Percent_Identity=71.4912280701754, Blast_Score=341, Evalue=3e-95,
Organism=Escherichia coli, GI1786247, Length=230, Percent_Identity=60, Blast_Score=284, Evalue=3e-78,
Organism=Escherichia coli, GI1790008, Length=230, Percent_Identity=60, Blast_Score=283, Evalue=6e-78,
Organism=Escherichia coli, GI1789164, Length=140, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: =5.1.3.4 [H]

Molecular weight: Translated: 25095; Mature: 25095

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLKQQVCQANMALPHHGLVTFTWGNVSAIDRQCGHVVIKPSGVAYEALTPDEMVVVDL
CHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCEEEECCCCEEEEECCCCCEEEEEE
DGRIVAGALRPSSDTPTHLALYRRYPQLGGIVHTHSTHATAWAQAGRAIPALGTTHADYF
CCEEEEEECCCCCCCCHHHHHHHHCHHHCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCC
DGDIPCTRALSTAEIDHDYEHHTGLVIIETLAQRDPGHMPGIVVHQHGSFAWGESAAQAV
CCCCCHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCCCCCCCEEEECCCCCCCCHHHHHHH
HNAVVLEEVAKMAWIAHSLNPRLAPMDRALHDKHFRRKHGPDAYYGQRG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MERLKQQVCQANMALPHHGLVTFTWGNVSAIDRQCGHVVIKPSGVAYEALTPDEMVVVDL
CHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHCCCEEEECCCCEEEEECCCCCEEEEEE
DGRIVAGALRPSSDTPTHLALYRRYPQLGGIVHTHSTHATAWAQAGRAIPALGTTHADYF
CCEEEEEECCCCCCCCHHHHHHHHCHHHCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCC
DGDIPCTRALSTAEIDHDYEHHTGLVIIETLAQRDPGHMPGIVVHQHGSFAWGESAAQAV
CCCCCHHHHHHHHHCCCCCCCCCCEEEEEEHHHCCCCCCCCEEEECCCCCCCCHHHHHHH
HNAVVLEEVAKMAWIAHSLNPRLAPMDRALHDKHFRRKHGPDAYYGQRG
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA