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The map label for this gene is ulaC [H]

Identifier: 238921508

GI number: 238921508

Start: 3546161

End: 3546646

Strand: Direct

Name: ulaC [H]

Synonym: NT01EI_3660

Alternate gene names: 238921508

Gene position: 3546161-3546646 (Clockwise)

Preceding gene: 238921507

Following gene: 238921509

Centisome position: 93.02

GC content: 65.84

Gene sequence:

>486_bases
ATGAAACTGCGAGACTCTCTGGCGGAAAACCACAGCATCCGCCTACAGGCCGAGGCGGCGGACTGGCCTGCGGCCGTCAG
GATCGGCGTGGATCTCCTGGTCAGCGCCGGCGTCGTAGAGCCACGCTACTACCGGGCCATCCTGGATAGCGTCGCGCGCT
TTGGCCCCTACTTTGTGCTGGCTCCGGGGCTGGCCATGCCGCACGGGCGCCCGGAAGAGGGGGTGATCAAGACCGGCTTT
GCACTGGTCACCCTGCGTACGCCGGTCGCGTTCGGCCATGAGGATAACGACCCGGTGGACATTCTGATCACCCTGGCCGC
CGTCGACGCCCACACCCATCAGGAGGTCGGTATCCGTCAGATCGTCGACCTGTTCGAGGATGAGGCCAACTTTACGCGCC
TGCGCGCCTGCCGCGCGCCTGCGCAGGTGCTGGCGCTCATCGATTCGGCAACCACCCCATCGCATCCCCAGGGAGTCTCC
TCATGA

Upstream 100 bases:

>100_bases
GGTCGGGGTGCGCAATATGCTGTCGGCAGACGAGTTCGGCCCCAGGCTGCTGGAGGTCATCCGCGCCCATTTCCCCCAGG
ACCTCAGCTAAGGAGCCATG

Downstream 100 bases:

>100_bases
TACCGTCCCTGCCTATGCTGCAAATCGCGCTGGATAACCCAACGCTGGAGGCGGCCTACCTCACCACCCGGCAGATCGTC
GAAGCCGTCGATATCATCGA

Product: PTS system L-ascorbate-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKLRDSLAENHSIRLQAEAADWPAAVRIGVDLLVSAGVVEPRYYRAILDSVARFGPYFVLAPGLAMPHGRPEEGVIKTGF
ALVTLRTPVAFGHEDNDPVDILITLAAVDAHTHQEVGIRQIVDLFEDEANFTRLRACRAPAQVLALIDSATTPSHPQGVS
S

Sequences:

>Translated_161_residues
MKLRDSLAENHSIRLQAEAADWPAAVRIGVDLLVSAGVVEPRYYRAILDSVARFGPYFVLAPGLAMPHGRPEEGVIKTGF
ALVTLRTPVAFGHEDNDPVDILITLAAVDAHTHQEVGIRQIVDLFEDEANFTRLRACRAPAQVLALIDSATTPSHPQGVS
S
>Mature_161_residues
MKLRDSLAENHSIRLQAEAADWPAAVRIGVDLLVSAGVVEPRYYRAILDSVARFGPYFVLAPGLAMPHGRPEEGVIKTGF
ALVTLRTPVAFGHEDNDPVDILITLAAVDAHTHQEVGIRQIVDLFEDEANFTRLRACRAPAQVLALIDSATTPSHPQGVS
S

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=148, Percent_Identity=78.3783783783784, Blast_Score=244, Evalue=2e-66,
Organism=Escherichia coli, GI1790755, Length=101, Percent_Identity=43.5643564356436, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1789302, Length=113, Percent_Identity=41.5929203539823, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17430; Mature: 17430

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRDSLAENHSIRLQAEAADWPAAVRIGVDLLVSAGVVEPRYYRAILDSVARFGPYFVL
CCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEE
APGLAMPHGRPEEGVIKTGFALVTLRTPVAFGHEDNDPVDILITLAAVDAHTHQEVGIRQ
CCCCCCCCCCCCCCEEECCEEEEEEECCEEECCCCCCCEEEEEEEEHHCCCCCHHHHHHH
IVDLFEDEANFTRLRACRAPAQVLALIDSATTPSHPQGVSS
HHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLRDSLAENHSIRLQAEAADWPAAVRIGVDLLVSAGVVEPRYYRAILDSVARFGPYFVL
CCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEE
APGLAMPHGRPEEGVIKTGFALVTLRTPVAFGHEDNDPVDILITLAAVDAHTHQEVGIRQ
CCCCCCCCCCCCCCEEECCEEEEEEECCEEECCCCCCCEEEEEEEEHHCCCCCHHHHHHH
IVDLFEDEANFTRLRACRAPAQVLALIDSATTPSHPQGVSS
HHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]