Click here to switch to the map view.

The map label for this gene is rsmB [H]

Identifier: 238921411

GI number: 238921411

Start: 3463786

End: 3465078

Strand: Direct

Name: rsmB [H]

Synonym: NT01EI_3562

Alternate gene names: 238921411

Gene position: 3463786-3465078 (Clockwise)

Preceding gene: 238921410

Following gene: 238921412

Centisome position: 90.86

GC content: 60.32

Gene sequence:

>1293_bases
ATGAAAACTACGTCCTATAACCTGCGCGCTATCGCCGCCCGCACGCTGAGTGCTGTCCTCGATCAGGGACAATCTCTGAG
TAGTGTCCTGCCCAGCGCCGCCCAATCGCTCAGCGAAAAAGATCGCGCCCTGTTACAGGAGCTATGCTTCGGCACCTTAC
GCGTATTGCCGCAGCTGGAGTGGATCCTACAACAGTTGATGGCCAAGGTACTGACCGGCAAACAACGTACGCTTCATTAC
CTGCTGATGGTTGGCCTGTATCAACTCGTGTATACCCGTATCCCACCCCATGCCGCCCTGGCCGAAACGGTCGATGGGGC
AACCGCCCTCAAACGGCCGCAGCTCAAGGGGCTGATCAATGGCGTGCTGCGCCAGTTCCAGCGCCAACAAGAGGCTCTAC
TGCTTCAGTCTCAGCAACATGCTAGCCACTGGTTACACCCCTCATGGTTGCTCAAGCGTCTGCAACACGCCTACCCGGAG
CAATGGCCTGCGATTGTCGAGGCCAATAACCAGCGTCCACCGATGTGGTTGCGTGTTAATCGCCGCCATCATACCCGCGA
CGCCTACCTCACTCTGTTACAGCAGGCTGGCATTGAAGCGGTGGCACATGACTACTTCCCCGATGCCATCCGTCTACTGG
TGGCACAGCCGGTAACACGCCTACCGGGATTCGAACACGGATGGGTCACGGTGCAGGATGCCAGCGCACAGCACAGCGTG
GATCTGCTCGATCCGCAGGATAGCGAGCAGATCCTCGATCTGTGCTGCGCGCCGGGCGGCAAAACCACCCATATCCTGGA
GGCGGCGCCTAAGGCCCACGTATTGGCTGTTGACGTCGATGAAACCCGCCTCAAGCGGGTGCGCGAAAACCTGCAGCGGC
TACAGCAGCATGCCGAGATCCGCTGCGGCGATGGGCGTACCCCTGACGAGTGGGCCAGCGGTCGGCAGTTCGACCGGATT
CTCTTGGACGCCCCCTGTTCAGCGACCGGCGTCATTCGCCGCCATCCGGACATTAAATGGCTGCGCCGCGACAGCGATAT
CACCGAGCTGGCCGCGCTGCAGCGCGAGATCATTGATGCCGTCTGGCCTTTTTTGAAATCCGGCGGCGTGATGGTCTACG
CCACCTGCTCCATTCTGCCGCAGGAAAACCAGGAGCAGATCCTCACCTTCCTGTCGCGCCACCCGGATGCGCGCCTGAGC
GACACCGGCGATATACAGCGGCCCGGCCTTCAGTTTTTGCCGCGGCCCGACGCGGGCGACGGCTTCTTTTACACTAAGCT
GATAAAAGCGTAA

Upstream 100 bases:

>100_bases
GCCCTCCGACGCGGCGTGTCCAATGCGCCATTTCTGCCCCAATCCGTGATAAACTCCAGGCCATATTCGACTTAACTTGC
AGCGACGGTCTCTTGCATCC

Downstream 100 bases:

>100_bases
TCCGCAGGCCGCCCGCCAGGCGGTCGGCGCTCGCCAAGCAGAGAACAGCATCATGAAAATTATCATTTTAGGTGCCGGCC
AGGTGGGGGGTACCCTAGCG

Product: 16S rRNA methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKTTSYNLRAIAARTLSAVLDQGQSLSSVLPSAAQSLSEKDRALLQELCFGTLRVLPQLEWILQQLMAKVLTGKQRTLHY
LLMVGLYQLVYTRIPPHAALAETVDGATALKRPQLKGLINGVLRQFQRQQEALLLQSQQHASHWLHPSWLLKRLQHAYPE
QWPAIVEANNQRPPMWLRVNRRHHTRDAYLTLLQQAGIEAVAHDYFPDAIRLLVAQPVTRLPGFEHGWVTVQDASAQHSV
DLLDPQDSEQILDLCCAPGGKTTHILEAAPKAHVLAVDVDETRLKRVRENLQRLQQHAEIRCGDGRTPDEWASGRQFDRI
LLDAPCSATGVIRRHPDIKWLRRDSDITELAALQREIIDAVWPFLKSGGVMVYATCSILPQENQEQILTFLSRHPDARLS
DTGDIQRPGLQFLPRPDAGDGFFYTKLIKA

Sequences:

>Translated_430_residues
MKTTSYNLRAIAARTLSAVLDQGQSLSSVLPSAAQSLSEKDRALLQELCFGTLRVLPQLEWILQQLMAKVLTGKQRTLHY
LLMVGLYQLVYTRIPPHAALAETVDGATALKRPQLKGLINGVLRQFQRQQEALLLQSQQHASHWLHPSWLLKRLQHAYPE
QWPAIVEANNQRPPMWLRVNRRHHTRDAYLTLLQQAGIEAVAHDYFPDAIRLLVAQPVTRLPGFEHGWVTVQDASAQHSV
DLLDPQDSEQILDLCCAPGGKTTHILEAAPKAHVLAVDVDETRLKRVRENLQRLQQHAEIRCGDGRTPDEWASGRQFDRI
LLDAPCSATGVIRRHPDIKWLRRDSDITELAALQREIIDAVWPFLKSGGVMVYATCSILPQENQEQILTFLSRHPDARLS
DTGDIQRPGLQFLPRPDAGDGFFYTKLIKA
>Mature_430_residues
MKTTSYNLRAIAARTLSAVLDQGQSLSSVLPSAAQSLSEKDRALLQELCFGTLRVLPQLEWILQQLMAKVLTGKQRTLHY
LLMVGLYQLVYTRIPPHAALAETVDGATALKRPQLKGLINGVLRQFQRQQEALLLQSQQHASHWLHPSWLLKRLQHAYPE
QWPAIVEANNQRPPMWLRVNRRHHTRDAYLTLLQQAGIEAVAHDYFPDAIRLLVAQPVTRLPGFEHGWVTVQDASAQHSV
DLLDPQDSEQILDLCCAPGGKTTHILEAAPKAHVLAVDVDETRLKRVRENLQRLQQHAEIRCGDGRTPDEWASGRQFDRI
LLDAPCSATGVIRRHPDIKWLRRDSDITELAALQREIIDAVWPFLKSGGVMVYATCSILPQENQEQILTFLSRHPDARLS
DTGDIQRPGLQFLPRPDAGDGFFYTKLIKA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=344, Percent_Identity=32.8488372093023, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI76150623, Length=344, Percent_Identity=32.8488372093023, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI32698918, Length=199, Percent_Identity=33.6683417085427, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI11545785, Length=221, Percent_Identity=28.9592760180996, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI301336155, Length=209, Percent_Identity=32.0574162679426, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI39995082, Length=209, Percent_Identity=32.0574162679426, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI2367212, Length=423, Percent_Identity=66.903073286052, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI87081985, Length=219, Percent_Identity=33.7899543378995, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=273, Percent_Identity=31.5018315018315, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25143471, Length=196, Percent_Identity=35.2040816326531, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324268, Length=270, Percent_Identity=32.962962962963, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=31.5533980582524, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI22024126, Length=236, Percent_Identity=33.8983050847458, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI21355201, Length=226, Percent_Identity=31.858407079646, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24668781, Length=189, Percent_Identity=29.1005291005291, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356513, Length=180, Percent_Identity=30, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920494, Length=153, Percent_Identity=30.0653594771242, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48561; Mature: 48561

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTSYNLRAIAARTLSAVLDQGQSLSSVLPSAAQSLSEKDRALLQELCFGTLRVLPQLE
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WILQQLMAKVLTGKQRTLHYLLMVGLYQLVYTRIPPHAALAETVDGATALKRPQLKGLIN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCHHHHHHHH
GVLRQFQRQQEALLLQSQQHASHWLHPSWLLKRLQHAYPEQWPAIVEANNQRPPMWLRVN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHEECCCCCCCEEEEEC
RRHHTRDAYLTLLQQAGIEAVAHDYFPDAIRLLVAQPVTRLPGFEHGWVTVQDASAQHSV
CCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
DLLDPQDSEQILDLCCAPGGKTTHILEAAPKAHVLAVDVDETRLKRVRENLQRLQQHAEI
CCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCE
RCGDGRTPDEWASGRQFDRILLDAPCSATGVIRRHPDIKWLRRDSDITELAALQREIIDA
ECCCCCCCHHHHCCCCHHHHHEECCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHH
VWPFLKSGGVMVYATCSILPQENQEQILTFLSRHPDARLSDTGDIQRPGLQFLPRPDAGD
HHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
GFFYTKLIKA
CHHHHHHHCC
>Mature Secondary Structure
MKTTSYNLRAIAARTLSAVLDQGQSLSSVLPSAAQSLSEKDRALLQELCFGTLRVLPQLE
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WILQQLMAKVLTGKQRTLHYLLMVGLYQLVYTRIPPHAALAETVDGATALKRPQLKGLIN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCHHHHHHHH
GVLRQFQRQQEALLLQSQQHASHWLHPSWLLKRLQHAYPEQWPAIVEANNQRPPMWLRVN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHEECCCCCCCEEEEEC
RRHHTRDAYLTLLQQAGIEAVAHDYFPDAIRLLVAQPVTRLPGFEHGWVTVQDASAQHSV
CCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCC
DLLDPQDSEQILDLCCAPGGKTTHILEAAPKAHVLAVDVDETRLKRVRENLQRLQQHAEI
CCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCE
RCGDGRTPDEWASGRQFDRILLDAPCSATGVIRRHPDIKWLRRDSDITELAALQREIIDA
ECCCCCCCHHHHCCCCHHHHHEECCCCCCCHHHCCCCHHHHHCCCCHHHHHHHHHHHHHH
VWPFLKSGGVMVYATCSILPQENQEQILTFLSRHPDARLSDTGDIQRPGLQFLPRPDAGD
HHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
GFFYTKLIKA
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA