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The map label for this gene is glyA

Identifier: 238921060

GI number: 238921060

Start: 3067637

End: 3068890

Strand: Reverse

Name: glyA

Synonym: NT01EI_3190

Alternate gene names: 238921060

Gene position: 3068890-3067637 (Counterclockwise)

Preceding gene: 238921062

Following gene: 238921058

Centisome position: 80.5

GC content: 58.13

Gene sequence:

>1254_bases
ATGTTAAAGCGTGAAATGAACATTGCCGATTACGATACCGAGCTGTGGCAGGCAATGCAGCAGGAAGTGACGCGTCAGGA
GCAGCATATTGAGCTGATCGCCTCGGAAAACTACACCAGCCCGCGGGTTATGCAGGCGCAGGGCTCCCAGCTGACCAATA
AATATGCCGAAGGTTACCCGGGTAAGCGTTACTACGGCGGTTGCCAGTATGTGGATCAGGTCGAGCAGCTGGCGATTGAT
CGCGCCAAGGCGCTGTTTGGCGCCGACTACGCCAACGTACAGCCCCACTCAGGTTCTCAGGCCAATTTCGCCGTCTATAC
CGCACTGCTGCAACCGGGCGATACGGTGCTGGGCATGAATCTGGCGCACGGCGGCCATCTGACGCATGGCTCTCCGGTTA
ACTTTTCCGGTAAGCTGTATAACGTGGTGCCTTACGGCATCGACGCGCATGGCCGTATCGACTATGACGATCTGGCGGCG
CAGGCGCAGCGGCACCGTCCGAAGATGATCATCGGCGGTTTCTCGGCCTATTCCGGCGTCGTGGACTGGGCGCGTATGCG
TGAGATCGCCAATAGCATCGGCGCCTATCTGTTTGTGGATATGGCGCATGTGGCGGGTCTGGTCGCTGCCGGGGTTTATC
CCAATCCGATTCCCCATGCCCACGTAGTGACCACCACTACTCACAAAACCCTGGCCGGTCCACGCGGCGGCTTGATCCTG
GCCAAGGGGTTGGATGAGACGATGTATAAAAAGCTGAACTCCGCCGTGTTCCCGGGAGCACAGGGGGGCCCGCTGATGCA
CGTGATCGCAGCCAAGGCGGTCGCTCTGAAAGAGGCGATGGAGCCGGAGTTTACCCGTTACCAGCAGCAGGTGGCCAAAA
ATGCCAAGGCGATGGTCGACGTCTTTCTGCAACGCGGCTATAAGGTCGTGTCCGGTGGCACGGAAAATCATCTATTTCTG
CTGGATCTGGTTGACAGGCAGATCACCGGCAAGGAGGCCGACGCCGCACTGGGACACGCCAATATTACGGTGAATAAAAA
CAGCGTGCCCAACGATCCGCAGAGTCCGTTCGTGACTTCCGGCATTCGCATCGGTACCCCTGCTATCACGCGCCGCGGTT
TTAAAGAGGCAGAGTCCCGTGAGCTGGCGGGATGGATGTGCGACGTGTTGGATAATATCCATGATGAGGCCACGATCGCC
TGCACCAAACAGAAGGTGCTGGCTCTTTGTGAACGTCTTCCGGTCTATGCCTGA

Upstream 100 bases:

>100_bases
AAAACATATTACACTGTGACGATTGCCCGTTGGGGTGGGGATGTCTTGGCTGTGAGGGCCAGATTTACTCAAAACCGGTT
AGCTGAGTCAGGAGAAGCGG

Downstream 100 bases:

>100_bases
CCGGCTGCCAGCCCTACCGAGCCCGCTACGCGCGGGCTTTTTACGTCAGTCGCTCCTGTCGTCGGCCTGCATCAGCCAGG
TGCCGGTACTGACGGCGATC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLKREMNIADYDTELWQAMQQEVTRQEQHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCQYVDQVEQLAID
RAKALFGADYANVQPHSGSQANFAVYTALLQPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNVVPYGIDAHGRIDYDDLAA
QAQRHRPKMIIGGFSAYSGVVDWARMREIANSIGAYLFVDMAHVAGLVAAGVYPNPIPHAHVVTTTTHKTLAGPRGGLIL
AKGLDETMYKKLNSAVFPGAQGGPLMHVIAAKAVALKEAMEPEFTRYQQQVAKNAKAMVDVFLQRGYKVVSGGTENHLFL
LDLVDRQITGKEADAALGHANITVNKNSVPNDPQSPFVTSGIRIGTPAITRRGFKEAESRELAGWMCDVLDNIHDEATIA
CTKQKVLALCERLPVYA

Sequences:

>Translated_417_residues
MLKREMNIADYDTELWQAMQQEVTRQEQHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCQYVDQVEQLAID
RAKALFGADYANVQPHSGSQANFAVYTALLQPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNVVPYGIDAHGRIDYDDLAA
QAQRHRPKMIIGGFSAYSGVVDWARMREIANSIGAYLFVDMAHVAGLVAAGVYPNPIPHAHVVTTTTHKTLAGPRGGLIL
AKGLDETMYKKLNSAVFPGAQGGPLMHVIAAKAVALKEAMEPEFTRYQQQVAKNAKAMVDVFLQRGYKVVSGGTENHLFL
LDLVDRQITGKEADAALGHANITVNKNSVPNDPQSPFVTSGIRIGTPAITRRGFKEAESRELAGWMCDVLDNIHDEATIA
CTKQKVLALCERLPVYA
>Mature_417_residues
MLKREMNIADYDTELWQAMQQEVTRQEQHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCQYVDQVEQLAID
RAKALFGADYANVQPHSGSQANFAVYTALLQPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNVVPYGIDAHGRIDYDDLAA
QAQRHRPKMIIGGFSAYSGVVDWARMREIANSIGAYLFVDMAHVAGLVAAGVYPNPIPHAHVVTTTTHKTLAGPRGGLIL
AKGLDETMYKKLNSAVFPGAQGGPLMHVIAAKAVALKEAMEPEFTRYQQQVAKNAKAMVDVFLQRGYKVVSGGTENHLFL
LDLVDRQITGKEADAALGHANITVNKNSVPNDPQSPFVTSGIRIGTPAITRRGFKEAESRELAGWMCDVLDNIHDEATIA
CTKQKVLALCERLPVYA

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=48.0099502487562, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=48.0099502487562, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=48.0099502487562, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=48.0099502487562, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI261862346, Length=401, Percent_Identity=47.1321695760599, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=46.1346633416459, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=43.4447300771208, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=87.5299760191847, Blast_Score=747, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=416, Percent_Identity=46.6346153846154, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI25144729, Length=416, Percent_Identity=46.6346153846154, Blast_Score=351, Evalue=5e-97,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=46.6334164588529, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=43.7810945273632, Blast_Score=328, Evalue=7e-91,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=46.75, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=46.75, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_EDWI9 (C5BEV2)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934575.1
- ProteinModelPortal:   C5BEV2
- GeneID:   7958708
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3190
- OMA:   PETERIN
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45527; Mature: 45527

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKREMNIADYDTELWQAMQQEVTRQEQHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEHHCCHHHHHHHHCCCC
GKRYYGGCQYVDQVEQLAIDRAKALFGADYANVQPHSGSQANFAVYTALLQPGDTVLGMN
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNVVPYGIDAHGRIDYDDLAAQAQRHRPKMIIGGFSAYSGV
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARMREIANSIGAYLFVDMAHVAGLVAAGVYPNPIPHAHVVTTTTHKTLAGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCEEE
AKGLDETMYKKLNSAVFPGAQGGPLMHVIAAKAVALKEAMEPEFTRYQQQVAKNAKAMVD
ECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLQRGYKVVSGGTENHLFLLDLVDRQITGKEADAALGHANITVNKNSVPNDPQSPFVTS
HHHHCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCEEC
GIRIGTPAITRRGFKEAESRELAGWMCDVLDNIHDEATIACTKQKVLALCERLPVYA
CEEECCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKREMNIADYDTELWQAMQQEVTRQEQHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEHHCCHHHHHHHHCCCC
GKRYYGGCQYVDQVEQLAIDRAKALFGADYANVQPHSGSQANFAVYTALLQPGDTVLGMN
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNVVPYGIDAHGRIDYDDLAAQAQRHRPKMIIGGFSAYSGV
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARMREIANSIGAYLFVDMAHVAGLVAAGVYPNPIPHAHVVTTTTHKTLAGPRGGLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCEEE
AKGLDETMYKKLNSAVFPGAQGGPLMHVIAAKAVALKEAMEPEFTRYQQQVAKNAKAMVD
ECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLQRGYKVVSGGTENHLFLLDLVDRQITGKEADAALGHANITVNKNSVPNDPQSPFVTS
HHHHCCCEEECCCCCCCEEEEEECCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCEEC
GIRIGTPAITRRGFKEAESRELAGWMCDVLDNIHDEATIACTKQKVLALCERLPVYA
CEEECCHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA