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The map label for this gene is nrfA

Identifier: 238921032

GI number: 238921032

Start: 3043165

End: 3044613

Strand: Reverse

Name: nrfA

Synonym: NT01EI_3162

Alternate gene names: 238921032

Gene position: 3044613-3043165 (Counterclockwise)

Preceding gene: 238921033

Following gene: 238921031

Centisome position: 79.86

GC content: 62.46

Gene sequence:

>1449_bases
ATGATTAAGGTAAGCAATGCGCTGCAGCGTATTTTGATCGGCGCCGCACTGGCGCTGTTTGGCGGCGGGGCCCAGGCGGC
CGCCGCGGATTCCGCCGCCGCGGCAGAGAAGGCGCCGCCGAAGATTGAGGCGCGCAACGATCGCTTCGCCGCGCCGCACC
CTGACCAGTATGCCTCCTGGAAGGCCACCGCCGAGCAGAGTGAAATTAGCGATGCGCTGGCGGAGGATCCACGCTTGGTG
ATCCTGTGGGCCGGCTACGCCTTTGCTAAGGACTATAACAAGCCGCGCGGCCACGCCTATGCGTTGACCGACGTGCGCGA
GACCCTGCGCACCGGTGCGCCGATGAAGCCGGAGGATGGGCCTCAGCCGATGGCCTGCTGGAGCTGTAAGAGTCCGGACG
TGGCGCGGGTGATCGCGGAGCAGGGAGAAGCGGCCTATTTCCACGGTATGTGGGCGCGCGGCGGGCCGGAGATCGTCAAC
GCCCTAGGCTGTGCCGACTGCCATAATACTGCCTCCGATGACTTTGCCGCCGGCAAACCGGCCCTGCAGCTGTCGCGCCC
CTACGCCGCGCGGGCGCTGGAGGCGATTGGCAAACCGTTCGATAAAGCCAACCGCATCGATCAGCAGTCGATGGTATGCG
GTCAGTGCCACGTGGAGTACTACTTCTCCGGCAAGGATAAAACCGTCAAATTCCCCTGGGATCAGGGCACCAGCGTCGAT
GAGATCGAGGCCTATTACGACGGTATTGCCTTTACCGACTGGGTTAACCCGCTCTCCAAGGCGCCGATGCTGAAGGCGCA
GCACCCGGATTATGAGACTTGGCGCGCCGGGATCCACGGACAGAACAACATCTCCTGCGTCGATTGCCATATGCCGAAGG
TGCAGAACGCCGAAGGCAAGCTGTACACCAGCCATAAGATAGGTAATCCATTCGATAGCTTTGAGCAGACCTGCCGGACC
TGTCATACCCAGGATAAAGCGACGCTGCAGCAGACCGTGGCCAAGCGCAAGCAGGATGTCAATGAGATCAAGCTGAAGGT
TGAGGATCAGCTGGTACGGGCGCACTTTGAGGCCAAGGCCGCTTGGGCCGCCGGTGCCAGCGAGGCCGAAATGCAGCCGA
TCCTGACCGACATTCGCCACGCCCAGTGGCGCTGGGACTTCTCTATTGCCTCCCACGGCGTGCATATGCATGCGCCGGAC
GTCGCGCTACGGATGTTGGGCACCGCGCTGGACAAGGCGGCCAATGCGCGTACTCAGCTGGTGCGGGTGCTGGCTAACCA
CGGCGTCAACGACGCGGTACCGCTGCCGGATATCTCCACCAAGGCGCTGGCGCAGCAGGCGCTGGGGATGAATATGCAGC
AGTTTACGCAGGAAAAGCAGACTTTCCTGAATACGGTTGTTCCTGAGTGGGAAGCCCAGGCGCGCAGCGCCGGGCGCCTG
GACCATTAA

Upstream 100 bases:

>100_bases
TCTATGTTCACCCTTACGCCCACGGATGTGCGATGTATAACTCATAACCTGCGTGCCATGACCCGTGTGCGACAGGGATA
TAACTCTGGATGGAATGCTT

Downstream 100 bases:

>100_bases
CCCCTGCGCCCCGCGCTGCGGGGCACTGCAGATGGAGTCATCATGAACCTACTGCGTTCGTTATTTACCGCCGGGGCGCT
GGTCAGTGGGCTGCTGCTTT

Product: hypothetical protein

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIKVSNALQRILIGAALALFGGGAQAAAADSAAAAEKAPPKIEARNDRFAAPHPDQYASWKATAEQSEISDALAEDPRLV
ILWAGYAFAKDYNKPRGHAYALTDVRETLRTGAPMKPEDGPQPMACWSCKSPDVARVIAEQGEAAYFHGMWARGGPEIVN
ALGCADCHNTASDDFAAGKPALQLSRPYAARALEAIGKPFDKANRIDQQSMVCGQCHVEYYFSGKDKTVKFPWDQGTSVD
EIEAYYDGIAFTDWVNPLSKAPMLKAQHPDYETWRAGIHGQNNISCVDCHMPKVQNAEGKLYTSHKIGNPFDSFEQTCRT
CHTQDKATLQQTVAKRKQDVNEIKLKVEDQLVRAHFEAKAAWAAGASEAEMQPILTDIRHAQWRWDFSIASHGVHMHAPD
VALRMLGTALDKAANARTQLVRVLANHGVNDAVPLPDISTKALAQQALGMNMQQFTQEKQTFLNTVVPEWEAQARSAGRL
DH

Sequences:

>Translated_482_residues
MIKVSNALQRILIGAALALFGGGAQAAAADSAAAAEKAPPKIEARNDRFAAPHPDQYASWKATAEQSEISDALAEDPRLV
ILWAGYAFAKDYNKPRGHAYALTDVRETLRTGAPMKPEDGPQPMACWSCKSPDVARVIAEQGEAAYFHGMWARGGPEIVN
ALGCADCHNTASDDFAAGKPALQLSRPYAARALEAIGKPFDKANRIDQQSMVCGQCHVEYYFSGKDKTVKFPWDQGTSVD
EIEAYYDGIAFTDWVNPLSKAPMLKAQHPDYETWRAGIHGQNNISCVDCHMPKVQNAEGKLYTSHKIGNPFDSFEQTCRT
CHTQDKATLQQTVAKRKQDVNEIKLKVEDQLVRAHFEAKAAWAAGASEAEMQPILTDIRHAQWRWDFSIASHGVHMHAPD
VALRMLGTALDKAANARTQLVRVLANHGVNDAVPLPDISTKALAQQALGMNMQQFTQEKQTFLNTVVPEWEAQARSAGRL
DH
>Mature_482_residues
MIKVSNALQRILIGAALALFGGGAQAAAADSAAAAEKAPPKIEARNDRFAAPHPDQYASWKATAEQSEISDALAEDPRLV
ILWAGYAFAKDYNKPRGHAYALTDVRETLRTGAPMKPEDGPQPMACWSCKSPDVARVIAEQGEAAYFHGMWARGGPEIVN
ALGCADCHNTASDDFAAGKPALQLSRPYAARALEAIGKPFDKANRIDQQSMVCGQCHVEYYFSGKDKTVKFPWDQGTSVD
EIEAYYDGIAFTDWVNPLSKAPMLKAQHPDYETWRAGIHGQNNISCVDCHMPKVQNAEGKLYTSHKIGNPFDSFEQTCRT
CHTQDKATLQQTVAKRKQDVNEIKLKVEDQLVRAHFEAKAAWAAGASEAEMQPILTDIRHAQWRWDFSIASHGVHMHAPD
VALRMLGTALDKAANARTQLVRVLANHGVNDAVPLPDISTKALAQQALGMNMQQFTQEKQTFLNTVVPEWEAQARSAGRL
DH

Specific function: Plays a role in nitrite reduction

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family

Homologues:

Organism=Escherichia coli, GI1790506, Length=447, Percent_Identity=73.1543624161074, Blast_Score=717, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFA_EDWI9 (C5BES4)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934547.1
- GeneID:   7958680
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3162
- OMA:   PWDMGTT
- ProtClustDB:   CLSK2813560
- GO:   GO:0006810
- HAMAP:   MF_01182
- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031
- PIRSF:   PIRSF000243
- TIGRFAMs:   TIGR03152

Pfam domain/function: PF02335 Cytochrom_C552

EC number: =1.7.2.2

Molecular weight: Translated: 52887; Mature: 52887

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 126-126 BINDING 129-129 BINDING 164-164 BINDING 167-167 BINDING 213-213 BINDING 216-216 BINDING 220-220 BINDING 268-268 BINDING 286-286 BINDING 289-289 BINDING 318-318 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVSNALQRILIGAALALFGGGAQAAAADSAAAAEKAPPKIEARNDRFAAPHPDQYASW
CCCHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
KATAEQSEISDALAEDPRLVILWAGYAFAKDYNKPRGHAYALTDVRETLRTGAPMKPEDG
CCHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCCCCCC
PQPMACWSCKSPDVARVIAEQGEAAYFHGMWARGGPEIVNALGCADCHNTASDDFAAGKP
CCCCEEECCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
ALQLSRPYAARALEAIGKPFDKANRIDQQSMVCGQCHVEYYFSGKDKTVKFPWDQGTSVD
CCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHCEEEEEEEECCCCCEEECCCCCCCCHH
EIEAYYDGIAFTDWVNPLSKAPMLKAQHPDYETWRAGIHGQNNISCVDCHMPKVQNAEGK
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
LYTSHKIGNPFDSFEQTCRTCHTQDKATLQQTVAKRKQDVNEIKLKVEDQLVRAHFEAKA
EEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AWAAGASEAEMQPILTDIRHAQWRWDFSIASHGVHMHAPDVALRMLGTALDKAANARTQL
HHHCCCCHHHHHHHHHHHHHHHEEEEEHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHH
VRVLANHGVNDAVPLPDISTKALAQQALGMNMQQFTQEKQTFLNTVVPEWEAQARSAGRL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
DH
CC
>Mature Secondary Structure
MIKVSNALQRILIGAALALFGGGAQAAAADSAAAAEKAPPKIEARNDRFAAPHPDQYASW
CCCHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
KATAEQSEISDALAEDPRLVILWAGYAFAKDYNKPRGHAYALTDVRETLRTGAPMKPEDG
CCHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCCCEEEHHHHHHHHHCCCCCCCCCC
PQPMACWSCKSPDVARVIAEQGEAAYFHGMWARGGPEIVNALGCADCHNTASDDFAAGKP
CCCCEEECCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
ALQLSRPYAARALEAIGKPFDKANRIDQQSMVCGQCHVEYYFSGKDKTVKFPWDQGTSVD
CCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHCEEEEEEEECCCCCEEECCCCCCCCHH
EIEAYYDGIAFTDWVNPLSKAPMLKAQHPDYETWRAGIHGQNNISCVDCHMPKVQNAEGK
HHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
LYTSHKIGNPFDSFEQTCRTCHTQDKATLQQTVAKRKQDVNEIKLKVEDQLVRAHFEAKA
EEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
AWAAGASEAEMQPILTDIRHAQWRWDFSIASHGVHMHAPDVALRMLGTALDKAANARTQL
HHHCCCCHHHHHHHHHHHHHHHEEEEEHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHH
VRVLANHGVNDAVPLPDISTKALAQQALGMNMQQFTQEKQTFLNTVVPEWEAQARSAGRL
HHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
DH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA