Click here to switch to the map view.

The map label for this gene is lepA [H]

Identifier: 238920907

GI number: 238920907

Start: 2923216

End: 2925009

Strand: Reverse

Name: lepA [H]

Synonym: NT01EI_3035

Alternate gene names: 238920907

Gene position: 2925009-2923216 (Counterclockwise)

Preceding gene: 238920908

Following gene: 238920906

Centisome position: 76.73

GC content: 58.31

Gene sequence:

>1794_bases
ATGAAGAACATACGTAATTTCTCCATTATCGCGCACATTGACCACGGTAAGTCGACGCTGTCTGACCGTATCATCCAGCT
CTGCGGCGGTCTGTCTGACCGTGAGATGGAGGCCCAGGTTCTGGACTCCATGGATCTGGAGCGCGAACGCGGTATCACCA
TCAAGGCGCAGAGCGTCACGCTCGACTATAAGGCGCAGGATGGGCAGGTGTATCAGCTCAACTTTATCGATACGCCGGGA
CACGTGGATTTCTCTTATGAGGTCTCCCGTTCGCTGGCCGCCTGTGAGGGGGCGCTGCTGGTGGTTGACGCCGGGCAGGG
GGTCGAAGCTCAGACCCTGGCCAACTGCTATACCGCCATTGAGATGGATCTGGAGGTGGTCCCGGTGTTGAACAAGATCG
ACCTGCCCGCCGCCGATCCGGATCGCGTGGCGCAGGAGATCGAAGACATCGTTGGTATTGATGCTACCGATGCGGTGCGT
TGTTCGGCCAAGACCGGGGTCGGTGTCGGCGATGTGCTGGAGCGTCTGGTGCGCGATATTCCAGCACCGGAAGGAGAGCC
AGAGGCGCCGCTGCAGGCGCTGATTATCGACTCTTGGTTCGATAACTATCTGGGCGTTGTATCGCTGATCCGCATTAAGA
ACGGTACGTTGCGTAAGGGCGATAAGGTCAAGGTGATGACCACCGGGCAGACCTATAACGCCGATCGCCTAGGGATCTTT
ACCCCGAAACAGGTCGACCGTGACGTACTGAACTGCGGTGAGGTCGGCTGGCTGGTTTGCGCCATCAAGGATATTCACGG
TGCGCCGGTTGGCGATACTCTGACCTTGGCCCGCAATCCGGCGGACGTCGCCCTGCCGGGCTTTAAGAAGGTGAAGCCGC
AGGTTTACGCCGGGCTGTTCCCGGTCAGCTCCGACGACTACGATGCCTTCCGTGATGCGTTGGGCAAGCTGAGCCTGAAT
GACGCCTCGTTGTTCTATGAGCCGGAAACCTCAACGGCGCTGGGCTTCGGCTTCCGCTGTGGCTTCCTGGGTCTGCTGCA
CATGGAGATCATTCAGGAGCGTCTGGAGCGTGAATACGATCTGGATCTGATCACCACCGCGCCGACGGTGGTGTATGAGG
TGGAGACCACCCGTAACGAAGTGATCTACGTAGACAGCCCCTCCAAGCTGCCGGCGGTGAACAATATTCAGGAGCTGCGC
GAGCCGATCGCCGAGTGCCACATGCTGCTGCCGCAGGAGTTCCTGGGCAACGTGATTACGCTCTGTATTGAGAAGCGTGG
CGTGCAGACCAATATGGTCTACCATGGTAATCAGGTGGCGCTGACCTATGAAATTCCGATGGCTGAGGTGGTGCTGGACT
TCTTTGACCGCCTGAAGTCTACCTCGCGCGGCTATGCGTCGCTGGATTACGGCTTTAAGCGCTTCCAGGCCTCGGATATG
GTGCGGGTTGATGTGATGGTCAACAGCGAGCGCGTCGATGCGCTGGCGCTGATCACCCACCGCGCTAACGCCAACGTGCG
TGGCCGTGAGCTGGTCGAGAAGATGAAAGAGCTGATCCCGCGTCAGCAGTTTGACATCGCCATTCAGGCGGCGATCGGCA
ACCAGATCATCGCTCGATCCACGGTGAAGCAGCTGCGTAAGAACGTACTGGCCAAGTGTTATGGCGGCGATGTCAGCCGT
AAGAAGAAGCTGCTGCAGAAGCAGAAAGAGGGTAAGAAACGCATGAAGCAGATCGGTAACGTTGAGCTGCCGCAGGAAGC
GTTCCTGGCCATTCTACACGTCGGTAAGGACTGA

Upstream 100 bases:

>100_bases
GATTCGGCGCCTGGCGCCACTTAACCCAAATCATCGCTTTGAGCCCGTCCGCGTGTCCTTAGGCGAGTAATTCAGAAATC
ATCAGGCATATATATTAATA

Downstream 100 bases:

>100_bases
TCGTTAGCCTGTCGGGCGCGGTGGATACCGCGCCCGCTGCGTTTATAAGCATTGATTTTAAGGAGTAATGGACATGGCAA
ATATGTTTGCCTTGATCCTG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQLCGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKAQDGQVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVR
CSAKTGVGVGDVLERLVRDIPAPEGEPEAPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMTTGQTYNADRLGIF
TPKQVDRDVLNCGEVGWLVCAIKDIHGAPVGDTLTLARNPADVALPGFKKVKPQVYAGLFPVSSDDYDAFRDALGKLSLN
DASLFYEPETSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTRNEVIYVDSPSKLPAVNNIQELR
EPIAECHMLLPQEFLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYGFKRFQASDM
VRVDVMVNSERVDALALITHRANANVRGRELVEKMKELIPRQQFDIAIQAAIGNQIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKD

Sequences:

>Translated_597_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQLCGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKAQDGQVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVR
CSAKTGVGVGDVLERLVRDIPAPEGEPEAPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMTTGQTYNADRLGIF
TPKQVDRDVLNCGEVGWLVCAIKDIHGAPVGDTLTLARNPADVALPGFKKVKPQVYAGLFPVSSDDYDAFRDALGKLSLN
DASLFYEPETSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTRNEVIYVDSPSKLPAVNNIQELR
EPIAECHMLLPQEFLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYGFKRFQASDM
VRVDVMVNSERVDALALITHRANANVRGRELVEKMKELIPRQQFDIAIQAAIGNQIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
>Mature_597_residues
MKNIRNFSIIAHIDHGKSTLSDRIIQLCGGLSDREMEAQVLDSMDLERERGITIKAQSVTLDYKAQDGQVYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAIEMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVR
CSAKTGVGVGDVLERLVRDIPAPEGEPEAPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMTTGQTYNADRLGIF
TPKQVDRDVLNCGEVGWLVCAIKDIHGAPVGDTLTLARNPADVALPGFKKVKPQVYAGLFPVSSDDYDAFRDALGKLSLN
DASLFYEPETSTALGFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTRNEVIYVDSPSKLPAVNNIQELR
EPIAECHMLLPQEFLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYGFKRFQASDM
VRVDVMVNSERVDALALITHRANANVRGRELVEKMKELIPRQQFDIAIQAAIGNQIIARSTVKQLRKNVLAKCYGGDVSR
KKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKD

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=46.4226289517471, Blast_Score=588, Evalue=1e-168,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.1127819548872, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=43.6241610738255, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI19923640, Length=137, Percent_Identity=45.985401459854, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=45.985401459854, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=42.3611111111111, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=35.625, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=42.7272727272727, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=42.7272727272727, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=42.7272727272727, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI53729339, Length=237, Percent_Identity=28.6919831223629, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729337, Length=237, Percent_Identity=28.6919831223629, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI217272892, Length=167, Percent_Identity=31.1377245508982, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI217272894, Length=167, Percent_Identity=31.1377245508982, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=93.6348408710218, Blast_Score=1116, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=515, Percent_Identity=28.9320388349515, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=34.8387096774194, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1789559, Length=289, Percent_Identity=28.719723183391, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=40.4255319148936, Blast_Score=485, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17556745, Length=159, Percent_Identity=35.8490566037736, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=37.6712328767123, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=36.9426751592357, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=35.8208955223881, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=35.8208955223881, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=28.3333333333333, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=226, Percent_Identity=27.4336283185841, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32566303, Length=244, Percent_Identity=26.6393442622951, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=45.9234608985025, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=43.75, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=43.75, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323098, Length=182, Percent_Identity=36.2637362637363, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=37.3913043478261, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=39.5833333333333, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=281, Percent_Identity=24.5551601423488, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319594, Length=281, Percent_Identity=24.5551601423488, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=246, Percent_Identity=25.609756097561, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=43.0948419301165, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.0248447204969, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=43.4782608695652, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=36.9127516778523, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=36.9127516778523, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=36.9127516778523, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.255033557047, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=35.3383458646617, Blast_Score=82, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28572034, Length=245, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66160; Mature: 66160

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQLCGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAQDGQVYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEECCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVRCSAKTGVGVGDVLERLVRDI
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PAPEGEPEAPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMTTGQTYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDRDVLNCGEVGWLVCAIKDIHGAPVGDTLTLARNPADVALPGFKKVKPQVYAGLF
CCHHHHHHHHCCCCHHEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHCCCHHHEEEE
PVSSDDYDAFRDALGKLSLNDASLFYEPETSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTRNEVIYVDSPSKLPAVNNIQELREPIAECHMLLPQEFLGNVIT
CEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYGFKRFQASDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCE
VRVDVMVNSERVDALALITHRANANVRGRELVEKMKELIPRQQFDIAIQAAIGNQIIARS
EEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MKNIRNFSIIAHIDHGKSTLSDRIIQLCGGLSDREMEAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAQDGQVYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAI
EEEECCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDLEVVPVLNKIDLPAADPDRVAQEIEDIVGIDATDAVRCSAKTGVGVGDVLERLVRDI
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PAPEGEPEAPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMTTGQTYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKQVDRDVLNCGEVGWLVCAIKDIHGAPVGDTLTLARNPADVALPGFKKVKPQVYAGLF
CCHHHHHHHHCCCCHHEEEEEEECCCCCCCCCEEEEECCCCCCCCCCCHHCCCHHHEEEE
PVSSDDYDAFRDALGKLSLNDASLFYEPETSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
LDLITTAPTVVYEVETTRNEVIYVDSPSKLPAVNNIQELREPIAECHMLLPQEFLGNVIT
CEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHH
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYGFKRFQASDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCE
VRVDVMVNSERVDALALITHRANANVRGRELVEKMKELIPRQQFDIAIQAAIGNQIIARS
EEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEHHCCHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA