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The map label for this gene is rumA

Identifier: 238920899

GI number: 238920899

Start: 2914175

End: 2915491

Strand: Reverse

Name: rumA

Synonym: NT01EI_3026

Alternate gene names: 238920899

Gene position: 2915491-2914175 (Counterclockwise)

Preceding gene: 238920901

Following gene: 238920898

Centisome position: 76.48

GC content: 63.25

Gene sequence:

>1317_bases
ATGGCTCAATTTTACACACCGGGGCGCCGTACCGCGACCCGGAAAATCCTGATTGTGACCGCAACCGATCTGGATGCCTT
TGGCCAGGGGGTGGCTCGCGAGCAGGGCAAGACGCTGTTTATCAGCGGGCTGCTGCCCGGTGAGCAGGCTGAGGTTATCC
TGAGCGAGGAGAAACGTCACTTTGCCCGCGGACGGGTTACGCGTTTAATCAGTCACAGCCCACAGAGGGTTGCGCCGCGT
TGTCCTCACGCTGGCGTCTGTGGTGGCTGTCAGCAGCAGCATGCGGCGATCGCCCTACAGCAGCAGAGCAAGTCGCAGGC
GCTGCAGCGTCTGATGGCGCGTGAGACCGGGGTGGATATTGCGCCGCAGGTGATCGCCGGAGAGCCTTATGGCTACCGGC
GGCGGGCCCGTTTTGGGCTACAGTTTAACGTGAAAACGCAGCGGGTGGTGCTGGGGTTCCGTCAGGCGGCCTCTAACGAT
CTGGTGGCCCTGAAAGCCTGCCCGGTGTTGGCGCCCGCGCTGGAAGCGCTGCTGCTGCCGCTGGGCGAATGCCTGTCGGC
GTTACGTGCGCGGCGCCGTCTGGGACACCTGGAGCTGGTGCTGGCCGACAATGGGCCGCTGATGGTGTTGCGCCATCTGG
ACCCGCTCTCTTTGTCGGATCGGGAGGCTCTGAGCGCCTTTGCCCGCCAGCGCGGACTCACGCTGTACCTGAGCGACGGC
GGCGCACCGCAGCGCCTGCTTGGCGAGGCACCCTATTACCAGATAGACGGGAGCCGCCTGGATTTTAACCCGCAGGATTT
TATCCAGGTGAATGCCGCGGTAAATGCGCAGATGGTCGGACAGGCTCTGGCCTGGCTGGATGTCAGGGCTGATGAGCGCG
TGCTGGATCTGTTCTGCGGCATGGGTAACTTTACCCTGCCGCTGGCGCGCCTCGCCCTGCGGGTGGTGGGCGTCGAGGGG
GTTCCCGGGCTGGTGGCGACGGCGCAGAATAATGCACGTAACAACGGGCTGAGTAATGTAGAATTCTTCCATCAGAATCT
GGAGGAGGACGTGACGCGTCAGGCCTGGGCGTCGCAGGGATTTGACAAGATTTTGCTCGATCCGGCGCGTGCCGGCGCCC
CCGGGGTGATGCAGCATATCGTTCGCCTGGCGCCGCGTCGGGTAGTCTATGTCTCCTGTAACCCGACCACGCTGGCCCGG
GACAGTCAGACGCTGCTGCAGGGCGGCTATCGGTTGACGCGTTTATGTATGCTGGACATGTTTCCCCACACGGGGCATTT
GGAGTCGATGGCGCTGTTTGAATCTGCGGCGCAATAG

Upstream 100 bases:

>100_bases
AGGCTGTATTTGGTCATGGCGTTCCGATGTGGGAGAATTGGCGGCAAACTATTATTGGGGTAAGCCCCGGCTTAACCCAC
CGCACAGATAAGCAATCGAC

Downstream 100 bases:

>100_bases
GCATTTGATAGGCAGTAGTCGAGAGGGAGAGAACCATGGTCGCGGTAAGAAGTGCTCATTTGAATACGGCTGGTGAATTT
GTCCCCGAGCAGTGGGTGGC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAQFYTPGRRTATRKILIVTATDLDAFGQGVAREQGKTLFISGLLPGEQAEVILSEEKRHFARGRVTRLISHSPQRVAPR
CPHAGVCGGCQQQHAAIALQQQSKSQALQRLMARETGVDIAPQVIAGEPYGYRRRARFGLQFNVKTQRVVLGFRQAASND
LVALKACPVLAPALEALLLPLGECLSALRARRRLGHLELVLADNGPLMVLRHLDPLSLSDREALSAFARQRGLTLYLSDG
GAPQRLLGEAPYYQIDGSRLDFNPQDFIQVNAAVNAQMVGQALAWLDVRADERVLDLFCGMGNFTLPLARLALRVVGVEG
VPGLVATAQNNARNNGLSNVEFFHQNLEEDVTRQAWASQGFDKILLDPARAGAPGVMQHIVRLAPRRVVYVSCNPTTLAR
DSQTLLQGGYRLTRLCMLDMFPHTGHLESMALFESAAQ

Sequences:

>Translated_438_residues
MAQFYTPGRRTATRKILIVTATDLDAFGQGVAREQGKTLFISGLLPGEQAEVILSEEKRHFARGRVTRLISHSPQRVAPR
CPHAGVCGGCQQQHAAIALQQQSKSQALQRLMARETGVDIAPQVIAGEPYGYRRRARFGLQFNVKTQRVVLGFRQAASND
LVALKACPVLAPALEALLLPLGECLSALRARRRLGHLELVLADNGPLMVLRHLDPLSLSDREALSAFARQRGLTLYLSDG
GAPQRLLGEAPYYQIDGSRLDFNPQDFIQVNAAVNAQMVGQALAWLDVRADERVLDLFCGMGNFTLPLARLALRVVGVEG
VPGLVATAQNNARNNGLSNVEFFHQNLEEDVTRQAWASQGFDKILLDPARAGAPGVMQHIVRLAPRRVVYVSCNPTTLAR
DSQTLLQGGYRLTRLCMLDMFPHTGHLESMALFESAAQ
>Mature_437_residues
AQFYTPGRRTATRKILIVTATDLDAFGQGVAREQGKTLFISGLLPGEQAEVILSEEKRHFARGRVTRLISHSPQRVAPRC
PHAGVCGGCQQQHAAIALQQQSKSQALQRLMARETGVDIAPQVIAGEPYGYRRRARFGLQFNVKTQRVVLGFRQAASNDL
VALKACPVLAPALEALLLPLGECLSALRARRRLGHLELVLADNGPLMVLRHLDPLSLSDREALSAFARQRGLTLYLSDGG
APQRLLGEAPYYQIDGSRLDFNPQDFIQVNAAVNAQMVGQALAWLDVRADERVLDLFCGMGNFTLPLARLALRVVGVEGV
PGLVATAQNNARNNGLSNVEFFHQNLEEDVTRQAWASQGFDKILLDPARAGAPGVMQHIVRLAPRRVVYVSCNPTTLARD
SQTLLQGGYRLTRLCMLDMFPHTGHLESMALFESAAQ

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=191, Percent_Identity=37.17277486911, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI51173878, Length=191, Percent_Identity=37.17277486911, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI269784680, Length=229, Percent_Identity=29.2576419213974, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI269784676, Length=229, Percent_Identity=29.2576419213974, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI20149688, Length=229, Percent_Identity=29.2576419213974, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI269784678, Length=229, Percent_Identity=29.2576419213974, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789148, Length=434, Percent_Identity=61.9815668202765, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1787083, Length=374, Percent_Identity=28.3422459893048, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790403, Length=350, Percent_Identity=28.2857142857143, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24666579, Length=198, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_EDWI9 (C5B8X6)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934414.1
- ProteinModelPortal:   C5B8X6
- GeneID:   7960304
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3026
- OMA:   IQVNQKV
- ProtClustDB:   PRK13168
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 47966; Mature: 47835

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 393-393 BINDING 269-269 BINDING 298-298 BINDING 303-303 BINDING 319-319 BINDING 346-346 BINDING 367-367

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFYTPGRRTATRKILIVTATDLDAFGQGVAREQGKTLFISGLLPGEQAEVILSEEKRH
CCCCCCCCCCCCCEEEEEEEECCHHHHCCCHHHHCCCEEEEEECCCCCCHHEEEHHHHHH
FARGRVTRLISHSPQRVAPRCPHAGVCGGCQQQHAAIALQQQSKSQALQRLMARETGVDI
HHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APQVIAGEPYGYRRRARFGLQFNVKTQRVVLGFRQAASNDLVALKACPVLAPALEALLLP
CCCEECCCCCCCHHHHEECEEECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHH
LGECLSALRARRRLGHLELVLADNGPLMVLRHLDPLSLSDREALSAFARQRGLTLYLSDG
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCC
GAPQRLLGEAPYYQIDGSRLDFNPQDFIQVNAAVNAQMVGQALAWLDVRADERVLDLFCG
CCHHHHHCCCCEEEECCCCCCCCCHHHEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHC
MGNFTLPLARLALRVVGVEGVPGLVATAQNNARNNGLSNVEFFHQNLEEDVTRQAWASQG
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FDKILLDPARAGAPGVMQHIVRLAPRRVVYVSCNPTTLARDSQTLLQGGYRLTRLCMLDM
CCEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCEECCCHHHHHHCCHHHHHHHHHHC
FPHTGHLESMALFESAAQ
CCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AQFYTPGRRTATRKILIVTATDLDAFGQGVAREQGKTLFISGLLPGEQAEVILSEEKRH
CCCCCCCCCCCCEEEEEEEECCHHHHCCCHHHHCCCEEEEEECCCCCCHHEEEHHHHHH
FARGRVTRLISHSPQRVAPRCPHAGVCGGCQQQHAAIALQQQSKSQALQRLMARETGVDI
HHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APQVIAGEPYGYRRRARFGLQFNVKTQRVVLGFRQAASNDLVALKACPVLAPALEALLLP
CCCEECCCCCCCHHHHEECEEECCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHH
LGECLSALRARRRLGHLELVLADNGPLMVLRHLDPLSLSDREALSAFARQRGLTLYLSDG
HHHHHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCC
GAPQRLLGEAPYYQIDGSRLDFNPQDFIQVNAAVNAQMVGQALAWLDVRADERVLDLFCG
CCHHHHHCCCCEEEECCCCCCCCCHHHEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHC
MGNFTLPLARLALRVVGVEGVPGLVATAQNNARNNGLSNVEFFHQNLEEDVTRQAWASQG
CCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FDKILLDPARAGAPGVMQHIVRLAPRRVVYVSCNPTTLARDSQTLLQGGYRLTRLCMLDM
CCEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCEECCCHHHHHHCCHHHHHHHHHHC
FPHTGHLESMALFESAAQ
CCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA