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The map label for this gene is insB [H]

Identifier: 238920855

GI number: 238920855

Start: 2871445

End: 2871822

Strand: Reverse

Name: insB [H]

Synonym: NT01EI_2981

Alternate gene names: 238920855

Gene position: 2871822-2871445 (Counterclockwise)

Preceding gene: 238920856

Following gene: 238920854

Centisome position: 75.33

GC content: 49.74

Gene sequence:

>378_bases
GTGGACAGTAAAGCCCGGCAGCACTGGCTCTGGTACGCGTACAATACCAAAACAGGCGGTGTACTGGCCTACATTTTTGG
TCCCAGAACCGATGAAAGCTGCCGGGAGCTACTGGCAGTGCTCATGCCATTCAACATTGGCATGAGCACCAGCGACGACC
GGGGCCGCGATGGCCGGGAGGTGCCGAAGGATAAGCATCTGACCGGCAAAATATTCACCCAACGCATTGAGTGTAATAAC
CTGATGCTCTGCACCATGATTAAGCGGCTTTCCCGTAAAATAATCAGTTTCTCACGCTCGGTTGAGATCCACGAAAAATT
CATCGGAGATTTTATCGGAAAATACATATTCTACTCATTGGATACATCGCCAACTTAA

Upstream 100 bases:

>100_bases
ATCCGGACGTTAAAAAACTCGCGTCAAAGCGAATAACGTCTTCACCTGTCGCCCATACTGATGTGGCGCTTATCTGCGAA
CTCGATGAGTAATGAGCTTT

Downstream 100 bases:

>100_bases
GGGATCTACTATTTTGCGCAGCTATTTATGATCGGCGCTAAGTTTATTTATTAATATATCTGTGGTAATAGAGGTGTAAT
GTATAAATTATATAAATGTA

Product: iso-IS1 ORF2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MDSKARQHWLWYAYNTKTGGVLAYIFGPRTDESCRELLAVLMPFNIGMSTSDDRGRDGREVPKDKHLTGKIFTQRIECNN
LMLCTMIKRLSRKIISFSRSVEIHEKFIGDFIGKYIFYSLDTSPT

Sequences:

>Translated_125_residues
MDSKARQHWLWYAYNTKTGGVLAYIFGPRTDESCRELLAVLMPFNIGMSTSDDRGRDGREVPKDKHLTGKIFTQRIECNN
LMLCTMIKRLSRKIISFSRSVEIHEKFIGDFIGKYIFYSLDTSPT
>Mature_125_residues
MDSKARQHWLWYAYNTKTGGVLAYIFGPRTDESCRELLAVLMPFNIGMSTSDDRGRDGREVPKDKHLTGKIFTQRIECNN
LMLCTMIKRLSRKIISFSRSVEIHEKFIGDFIGKYIFYSLDTSPT

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=110, Percent_Identity=39.0909090909091, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788203, Length=110, Percent_Identity=39.0909090909091, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1786203, Length=110, Percent_Identity=39.0909090909091, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1787223, Length=110, Percent_Identity=39.0909090909091, Blast_Score=83, Evalue=5e-18,
Organism=Escherichia coli, GI1786470, Length=110, Percent_Identity=39.0909090909091, Blast_Score=82, Evalue=8e-18,
Organism=Escherichia coli, GI1786459, Length=110, Percent_Identity=39.0909090909091, Blast_Score=82, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 14477; Mature: 14477

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSKARQHWLWYAYNTKTGGVLAYIFGPRTDESCRELLAVLMPFNIGMSTSDDRGRDGRE
CCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VPKDKHLTGKIFTQRIECNNLMLCTMIKRLSRKIISFSRSVEIHEKFIGDFIGKYIFYSL
CCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEC
DTSPT
CCCCC
>Mature Secondary Structure
MDSKARQHWLWYAYNTKTGGVLAYIFGPRTDESCRELLAVLMPFNIGMSTSDDRGRDGRE
CCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VPKDKHLTGKIFTQRIECNNLMLCTMIKRLSRKIISFSRSVEIHEKFIGDFIGKYIFYSL
CCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEEC
DTSPT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]