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The map label for this gene is ubiF [H]

Identifier: 238920796

GI number: 238920796

Start: 2809751

End: 2810926

Strand: Reverse

Name: ubiF [H]

Synonym: NT01EI_2922

Alternate gene names: 238920796

Gene position: 2810926-2809751 (Counterclockwise)

Preceding gene: 238920798

Following gene: 238920792

Centisome position: 73.73

GC content: 67.6

Gene sequence:

>1176_bases
ATGGTTCAGGAAGCTGGCGGTATTGTGGTGGTCGGCGGTGGGATGGTGGGTGCGGCGGCGGCTCTGGGGCTGGCGCAGGC
CGGTCTGCGGGTGACTGTGCTGGAAAACAGCCCGCCGCCATCGTTTGTACCGGACAGCGATCCCGATCTGCGCATCTCCG
CCATCAGCATGGCCAGCGTGCGCCTGCTGGAGCGGCTCGGGGTGTGGCCGGCGGTGCGGGCGATGCGCGCGGTGCCCTAC
CGCGGGCTGGAGACCTGGGAATGGGCATCGTCAACGGTCTCATTTCGTGCCGAGACGCTCGGCCTGCCATGCCTGGGCTT
TATGCTGGAGAACCGGGTGCTGCAGCGCGCGCTGTGGCAGGCGCTGGAGGCCTGCGAAGGCGTTACGTTATATACCGGGG
CGTCGCTGCGTCAACTGCGACGCGATGGGCAGGGCTGGCAGCTGGCGTTGGATAACGGTGAAGGGCTGTATGCCGGGTTG
GTCATCGCGGCGGATGGCGCTGAGTCCCGGGTACGCAGTCAGGCCGGTATCGGTGTCCACGGCTGGCAGTACCGCCACGA
CTGCATGCTGATCAGCGTCAGGACACCGTTGGCGCCGCAGGATGTCACCTGGCAACAGTTTACCCCTGACGGTCCGCGGG
CCTTTCTTCCCCTGTGGCCCGGCTGGGGCTCGCTGGTGTGGTATGACAGCCCGGCGCGTATCCGCCAGCTGCTGGCGATG
TCGCTGGCGCAGTTGAATGAGGCGATCCTGCGTACGTTTCCCGCACGCCTGGGGCCGGTGCAGGCGGTGGCCGCGGCGGC
GTTTCCGCTGATCCGGCGCCACGCGCAACGTTACGTAGATGAGGGACTGCTGCTGCTGGGCGATGCGGCGCACACCATTA
ACCCGCTCGCCGGGCAGGGGGTTAATCTGGGCTACCGTGACGTATCGGCGCTGCTGGAGGTGGTACCGGCAGCGGCAGCG
TGCGGTGAGGCGTGGCAGACGCAGGCGGTGCTGCAGCGTTATCAGCGCCGTCGCCTGGGCGACAACCTGCTGATGCAGAG
CGGGATGGACCTCTTGTACGGCGCCTTCAGCAACCGTCTACCGCCGCTGATGCTGCTGCGCAATCTGGGGCTAATGGCGG
CGGAGCGCGCCGGTGGGCTCAAGGCACGCGCGCTGCGCTACGCTATCGGGCTGTAG

Upstream 100 bases:

>100_bases
TGTAATCGCTTGGCGCTGTCGTGACCAGTCAGATAGCAGGGGGCAAGGGAGCGGAAATCCGTTACACTAGCGCAAAACGT
GCGGCGACAGGGGAGAGGGC

Downstream 100 bases:

>100_bases
GCGATGGCGATGGGCGGTGCGTCGTGTGGCGCGCCGCGCTGTCAAAGGCGTTGGCAAACGGCAGGCAAAAAAAAAGCCCA
CCCGAAGGTGAGCTCTCTTA

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MVQEAGGIVVVGGGMVGAAAALGLAQAGLRVTVLENSPPPSFVPDSDPDLRISAISMASVRLLERLGVWPAVRAMRAVPY
RGLETWEWASSTVSFRAETLGLPCLGFMLENRVLQRALWQALEACEGVTLYTGASLRQLRRDGQGWQLALDNGEGLYAGL
VIAADGAESRVRSQAGIGVHGWQYRHDCMLISVRTPLAPQDVTWQQFTPDGPRAFLPLWPGWGSLVWYDSPARIRQLLAM
SLAQLNEAILRTFPARLGPVQAVAAAAFPLIRRHAQRYVDEGLLLLGDAAHTINPLAGQGVNLGYRDVSALLEVVPAAAA
CGEAWQTQAVLQRYQRRRLGDNLLMQSGMDLLYGAFSNRLPPLMLLRNLGLMAAERAGGLKARALRYAIGL

Sequences:

>Translated_391_residues
MVQEAGGIVVVGGGMVGAAAALGLAQAGLRVTVLENSPPPSFVPDSDPDLRISAISMASVRLLERLGVWPAVRAMRAVPY
RGLETWEWASSTVSFRAETLGLPCLGFMLENRVLQRALWQALEACEGVTLYTGASLRQLRRDGQGWQLALDNGEGLYAGL
VIAADGAESRVRSQAGIGVHGWQYRHDCMLISVRTPLAPQDVTWQQFTPDGPRAFLPLWPGWGSLVWYDSPARIRQLLAM
SLAQLNEAILRTFPARLGPVQAVAAAAFPLIRRHAQRYVDEGLLLLGDAAHTINPLAGQGVNLGYRDVSALLEVVPAAAA
CGEAWQTQAVLQRYQRRRLGDNLLMQSGMDLLYGAFSNRLPPLMLLRNLGLMAAERAGGLKARALRYAIGL
>Mature_391_residues
MVQEAGGIVVVGGGMVGAAAALGLAQAGLRVTVLENSPPPSFVPDSDPDLRISAISMASVRLLERLGVWPAVRAMRAVPY
RGLETWEWASSTVSFRAETLGLPCLGFMLENRVLQRALWQALEACEGVTLYTGASLRQLRRDGQGWQLALDNGEGLYAGL
VIAADGAESRVRSQAGIGVHGWQYRHDCMLISVRTPLAPQDVTWQQFTPDGPRAFLPLWPGWGSLVWYDSPARIRQLLAM
SLAQLNEAILRTFPARLGPVQAVAAAAFPLIRRHAQRYVDEGLLLLGDAAHTINPLAGQGVNLGYRDVSALLEVVPAAAA
CGEAWQTQAVLQRYQRRRLGDNLLMQSGMDLLYGAFSNRLPPLMLLRNLGLMAAERAGGLKARALRYAIGL

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=382, Percent_Identity=30.1047120418848, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI32967307, Length=332, Percent_Identity=30.1204819277108, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1786883, Length=391, Percent_Identity=63.4271099744246, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1789273, Length=387, Percent_Identity=39.0180878552972, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1789274, Length=372, Percent_Identity=31.989247311828, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25146631, Length=427, Percent_Identity=28.3372365339578, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321694, Length=449, Percent_Identity=24.9443207126949, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19920760, Length=418, Percent_Identity=28.7081339712919, Blast_Score=161, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 42321; Mature: 42321

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQEAGGIVVVGGGMVGAAAALGLAQAGLRVTVLENSPPPSFVPDSDPDLRISAISMASV
CCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHHH
RLLERLGVWPAVRAMRAVPYRGLETWEWASSTVSFRAETLGLPCLGFMLENRVLQRALWQ
HHHHHHCCCHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ALEACEGVTLYTGASLRQLRRDGQGWQLALDNGEGLYAGLVIAADGAESRVRSQAGIGVH
HHHHHCCCEEECCHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCHHHHHHHHCCCCCC
GWQYRHDCMLISVRTPLAPQDVTWQQFTPDGPRAFLPLWPGWGSLVWYDSPARIRQLLAM
CEEEECCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHHH
SLAQLNEAILRTFPARLGPVQAVAAAAFPLIRRHAQRYVDEGLLLLGDAAHTINPLAGQG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCCCC
VNLGYRDVSALLEVVPAAAACGEAWQTQAVLQRYQRRRLGDNLLMQSGMDLLYGAFSNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHCCC
PPLMLLRNLGLMAAERAGGLKARALRYAIGL
CHHHHHHHCCHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVQEAGGIVVVGGGMVGAAAALGLAQAGLRVTVLENSPPPSFVPDSDPDLRISAISMASV
CCCCCCCEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEHHHHHHH
RLLERLGVWPAVRAMRAVPYRGLETWEWASSTVSFRAETLGLPCLGFMLENRVLQRALWQ
HHHHHHCCCHHHHHHHHCCCCCCCCHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ALEACEGVTLYTGASLRQLRRDGQGWQLALDNGEGLYAGLVIAADGAESRVRSQAGIGVH
HHHHHCCCEEECCHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCHHHHHHHHCCCCCC
GWQYRHDCMLISVRTPLAPQDVTWQQFTPDGPRAFLPLWPGWGSLVWYDSPARIRQLLAM
CEEEECCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCHHHHHHHHHH
SLAQLNEAILRTFPARLGPVQAVAAAAFPLIRRHAQRYVDEGLLLLGDAAHTINPLAGQG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHCCCCCCCC
VNLGYRDVSALLEVVPAAAACGEAWQTQAVLQRYQRRRLGDNLLMQSGMDLLYGAFSNRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCHHHHHHHHHCCC
PPLMLLRNLGLMAAERAGGLKARALRYAIGL
CHHHHHHHCCHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]