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The map label for this gene is manX [H]

Identifier: 238920699

GI number: 238920699

Start: 2703762

End: 2704202

Strand: Reverse

Name: manX [H]

Synonym: NT01EI_2812

Alternate gene names: 238920699

Gene position: 2704202-2703762 (Counterclockwise)

Preceding gene: 238920700

Following gene: 238920698

Centisome position: 70.93

GC content: 64.17

Gene sequence:

>441_bases
ATGATTGGTCTTGTCATTTCCGGGCACATCCATTTTGCAACCGGCATGGCCTCCGCCGTGCACGCCATCGCCGGCGATCA
GCCGCAGATGCGCTTTATCGATTTTGTTGAATCCATGTCGACCGACGATCTGGAGGAGGCGCTGCGCCAGGCGGCCGATG
AGGTCGACGACGGGGAGGGGGTGCTGTTCCTGACCGACCTGCCGGGGGGCTCGCCATGCAACCGCGCGCTGAACGTACTG
ATGCGCCGTGAGGGCGTTGAGGTGCTGGCCGGGGTCAACCTGCCGATGATCGCCAACGCCGCCTTTGAGCGCGACGGTGC
GACGCTGGCGGAGCTGGTGGCCGTTCTGCAGGAGATTGGCGCCAGCAGCGTACAGAATATGCGTCAGACGCTGGAAGCCG
CGATGGCCCCCGTCGAATGCGAAGCGAGCGACGGACTGTAA

Upstream 100 bases:

>100_bases
GCGTCGTCGGCCACCTGATGGGTCTTCTGTAATCGTCGCTCCCCGCGCCATCATCGATGGGCGGGGAGTTTACCAATGAG
TCAGTTGTAAGGGTTATTCC

Downstream 100 bases:

>100_bases
ACCATGTTAACCGCCTACCTGGCCGACAGCGTATTTACCCCCCGGGGGATTGAACGGGACGTCGCCGTGCTGGTTGACGA
CGGCGTGATCACCGGTATCG

Product: PTS system, N-acetylgalactosamine-specific IIA component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MIGLVISGHIHFATGMASAVHAIAGDQPQMRFIDFVESMSTDDLEEALRQAADEVDDGEGVLFLTDLPGGSPCNRALNVL
MRREGVEVLAGVNLPMIANAAFERDGATLAELVAVLQEIGASSVQNMRQTLEAAMAPVECEASDGL

Sequences:

>Translated_146_residues
MIGLVISGHIHFATGMASAVHAIAGDQPQMRFIDFVESMSTDDLEEALRQAADEVDDGEGVLFLTDLPGGSPCNRALNVL
MRREGVEVLAGVNLPMIANAAFERDGATLAELVAVLQEIGASSVQNMRQTLEAAMAPVECEASDGL
>Mature_146_residues
MIGLVISGHIHFATGMASAVHAIAGDQPQMRFIDFVESMSTDDLEEALRQAADEVDDGEGVLFLTDLPGGSPCNRALNVL
MRREGVEVLAGVNLPMIANAAFERDGATLAELVAVLQEIGASSVQNMRQTLEAAMAPVECEASDGL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=128, Percent_Identity=34.375, Blast_Score=73, Evalue=8e-15,
Organism=Escherichia coli, GI1786321, Length=127, Percent_Identity=32.2834645669291, Blast_Score=66, Evalue=8e-13,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 15384; Mature: 15384

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLVISGHIHFATGMASAVHAIAGDQPQMRFIDFVESMSTDDLEEALRQAADEVDDGEG
CEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
VLFLTDLPGGSPCNRALNVLMRREGVEVLAGVNLPMIANAAFERDGATLAELVAVLQEIG
EEEEEECCCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHC
ASSVQNMRQTLEAAMAPVECEASDGL
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIGLVISGHIHFATGMASAVHAIAGDQPQMRFIDFVESMSTDDLEEALRQAADEVDDGEG
CEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
VLFLTDLPGGSPCNRALNVLMRREGVEVLAGVNLPMIANAAFERDGATLAELVAVLQEIG
EEEEEECCCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHC
ASSVQNMRQTLEAAMAPVECEASDGL
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]