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The map label for this gene is mepA [H]

Identifier: 238920641

GI number: 238920641

Start: 2652351

End: 2653175

Strand: Reverse

Name: mepA [H]

Synonym: NT01EI_2753

Alternate gene names: 238920641

Gene position: 2653175-2652351 (Counterclockwise)

Preceding gene: 238920642

Following gene: 238920624

Centisome position: 69.59

GC content: 66.55

Gene sequence:

>825_bases
GTGAGTAAATGTGCGTGGTTATGGCTGGCGGCGCTGTGCGCGTTGCCGGCGCTGGCTCAGACGCCCTGGCAACGCATTAC
CCAACCGGTGGCCGGTGCGCCGCAGGCCATCGGCAGTTACGCCAATGGCTGTATCATCGGCGCCCGGGCACTGCCGCTGG
CGTCGGAGAACTATCAGGTTATGCGCAGTGAGCAGCTGCGCTACTTCGGCCATCCGACGCTGTTGGCGTTCGTGGAGCGT
CTGGGTACCGAGGTCCGTGCGCAGCATCTGGGTACGCTGTTGATCGGCGATATGGCGATGCCGGCCGGCGGGAGCTTCAA
CGGCGGTCACGCCAGCCATCAGTCGGGGCTGGATGTGGACATCTGGCTACAGCTGCCCCAGGTGCGCTGGACGCAGCAGC
AGCTGCGGCAGCCGGTACCACAGGATCTGGTGGCCGTCGGCGCTGACAGGGTGATCCCATCGCGCTGGACGCCGCAGATC
GGTAAGCTGATTAGGCTGGCGGCGCAGGATCCTCAGGTGGTGCGCATTTTTGTCAACCCGGCGATCAAGCGTCAGCTGTG
CGACACGGCCGACGGCGATCGCGGCTGGCTGCATAAAGTACGCCCGTGGTTTGCCCATCGTGCCCATATGCATGTGCGCC
TGCGTTGTCCACCGGGTAGCGCGGCCTGTGAGGCGCAAACGCCGCCTCCTCCGGGCGATGGCTGCGGTGCGGAGCTGGCC
AGCTGGTTCTTGCCTCCGCCGTCGCGCAGTGCGCCGTCCAGGCCACAGACGCCACCGCCGCTGCCACCGACGTGTCAGGC
GCTGCTGGCGCAACTTCAGGGCTAA

Upstream 100 bases:

>100_bases
GCAATGATGGCACTGGTGCTGATGGATCATCTGCTGCGCCAGCGTGCGCAGTGTGCGGATGTCGCCTGTACGGTGCCACG
CTGGTAAAGGAGTGGTCACA

Downstream 100 bases:

>100_bases
GCCGCCGCTCGCCGCGCCTGCCGTGGTCTTCAGGCCATTATCGCGACACCGGGCCATCAGTGAGAGGTGCTGGCAGAGTG
CCGGTACCGCGTTAGTTAGG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSKCAWLWLAALCALPALAQTPWQRITQPVAGAPQAIGSYANGCIIGARALPLASENYQVMRSEQLRYFGHPTLLAFVER
LGTEVRAQHLGTLLIGDMAMPAGGSFNGGHASHQSGLDVDIWLQLPQVRWTQQQLRQPVPQDLVAVGADRVIPSRWTPQI
GKLIRLAAQDPQVVRIFVNPAIKRQLCDTADGDRGWLHKVRPWFAHRAHMHVRLRCPPGSAACEAQTPPPPGDGCGAELA
SWFLPPPSRSAPSRPQTPPPLPPTCQALLAQLQG

Sequences:

>Translated_274_residues
MSKCAWLWLAALCALPALAQTPWQRITQPVAGAPQAIGSYANGCIIGARALPLASENYQVMRSEQLRYFGHPTLLAFVER
LGTEVRAQHLGTLLIGDMAMPAGGSFNGGHASHQSGLDVDIWLQLPQVRWTQQQLRQPVPQDLVAVGADRVIPSRWTPQI
GKLIRLAAQDPQVVRIFVNPAIKRQLCDTADGDRGWLHKVRPWFAHRAHMHVRLRCPPGSAACEAQTPPPPGDGCGAELA
SWFLPPPSRSAPSRPQTPPPLPPTCQALLAQLQG
>Mature_273_residues
SKCAWLWLAALCALPALAQTPWQRITQPVAGAPQAIGSYANGCIIGARALPLASENYQVMRSEQLRYFGHPTLLAFVERL
GTEVRAQHLGTLLIGDMAMPAGGSFNGGHASHQSGLDVDIWLQLPQVRWTQQQLRQPVPQDLVAVGADRVIPSRWTPQIG
KLIRLAAQDPQVVRIFVNPAIKRQLCDTADGDRGWLHKVRPWFAHRAHMHVRLRCPPGSAACEAQTPPPPGDGCGAELAS
WFLPPPSRSAPSRPQTPPPLPPTCQALLAQLQG

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=271, Percent_Identity=61.6236162361624, Blast_Score=336, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 29792; Mature: 29661

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKCAWLWLAALCALPALAQTPWQRITQPVAGAPQAIGSYANGCIIGARALPLASENYQV
CCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHH
MRSEQLRYFGHPTLLAFVERLGTEVRAQHLGTLLIGDMAMPAGGSFNGGHASHQSGLDVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPQVRWTQQQLRQPVPQDLVAVGADRVIPSRWTPQIGKLIRLAAQDPQVVRIFVNP
EEEECCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECH
AIKRQLCDTADGDRGWLHKVRPWFAHRAHMHVRLRCPPGSAACEAQTPPPPGDGCGAELA
HHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
SWFLPPPSRSAPSRPQTPPPLPPTCQALLAQLQG
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SKCAWLWLAALCALPALAQTPWQRITQPVAGAPQAIGSYANGCIIGARALPLASENYQV
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHCCCEEEECCCCCCCCCCHHH
MRSEQLRYFGHPTLLAFVERLGTEVRAQHLGTLLIGDMAMPAGGSFNGGHASHQSGLDVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPQVRWTQQQLRQPVPQDLVAVGADRVIPSRWTPQIGKLIRLAAQDPQVVRIFVNP
EEEECCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECH
AIKRQLCDTADGDRGWLHKVRPWFAHRAHMHVRLRCPPGSAACEAQTPPPPGDGCGAELA
HHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHH
SWFLPPPSRSAPSRPQTPPPLPPTCQALLAQLQG
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA