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The map label for this gene is hybC [H]

Identifier: 238920545

GI number: 238920545

Start: 2550710

End: 2552413

Strand: Reverse

Name: hybC [H]

Synonym: NT01EI_2657

Alternate gene names: 238920545

Gene position: 2552413-2550710 (Counterclockwise)

Preceding gene: 238920546

Following gene: 238920544

Centisome position: 66.95

GC content: 58.98

Gene sequence:

>1704_bases
ATGAGCCAACGTATTACTATCGACCCGATTACCCGCATCGAGGGCCATCTGCGCATTGACTGCGAGATCGAAAACGGCAA
AGTGACTAAAGCCTGGTCATCCGGCACTATGTGGCGTGGGATGGAAGACATCGTTAAGGGTAATGACCCACGCGATGCCT
GGATGATCGTTCAGCGTATCTGCGGCGTATGTACCACCATCCACGCTATCGCCTCCGTCCGCGCGGTCGAAAGCGCCGTT
GGGGCCAAGGTCCCGGTGAACGCGCAGTATATCCGTAACCTGATCCTGTCGGCGCACAGCATTCACGACCATATCGTGCA
CTTTTATCAGCTGTCGGCGATGGACTGGGTGGACATCACCTCCGCGCTGAAAGCCGATCCGAACAAGTGTGAGGCGATGC
TGCAGGGCGTTTCCGCCTGGCAGCTGAACAGCGCCGGCGAGTTCGCCAAGGTACAGCAGAAGATCCGTAATCTGGTCGAC
AGCGGCCAACTGGGGATCTTTGCCAACGGCTACTGGGGCCACAAGGAGATGAAGCTCTCTCCAGAGGTGAACCTGATCGC
TGTCGCCCACTACCTGCAGGCACTGGAGTGTCAGCGTGATGCCAACCGCGTCGTTGCCATCCTGGGCGGTAAGTCTCCGC
ATATCCAGAACCTGGCGGTCGGTGGGGTAGCCAACCCGATCAACCTGAACGCACCGAGCGTGCTCAACCTGGAGCGCCTG
ATGTACGTCAAGATGTTTATTGAACGTCTGGGCGACTTTATTGAGCAGGTGTACAAGGTGGATGCGGCGATCATCGCGGC
CAACTACCCGGAGTGGATGACGCTGGGCCGCGGCGCTCAGCACTACCTGAGCGTGCCGGAGCTGCCGACTGACGGCGACG
GTGGTAGCTTCCTGCTGCCGGGCGGCTACATTGAAAACGGCGACATGGCCAACTACCGTCCGATCAACAGCCAGCAGGAT
CCGTGGCTGATTGCCGGTATCGCCGAATCCAGCAAACACTCCTGGTACCAGGATGATGCCAACCTGAAGCCGTGGGAAGG
GAAAACCGAGCCGAACTACACCGGCTGGCAGGAGGACGGTAAGTACTCCTGGGTGAAATCACCGACCCTGTACGGCAAGG
TGGTCGAAGTCGGCCCGCTGGCCGATCTGCTGGTGAAGCTGGCGGCCAAGCATGAAGACACCGTCAAACATTACAACGAA
GTCAATGGGCTGTACCAGAAGCTGACCGGCAAGACGCTGCAGGTCGAGCAGCTGCACTCCACCATGGGCCGTATCATTGG
CCGCGCGGTGCGTTGCTGCGTGCTGAAAGATACCCTGATGATGCAGTGGCAGGCGCTGATCGACAATATTGGTAAGGGCG
ATCACGTGGCCTATATCCCGGCTGAAATTGACCCGAACAAAGAGTACCGTGGCGTTGGCTTCGAAGAGGCGCCGCGCGGT
GCACTGTCGCACTGGATCGTCATCAAGGGTGGCAAGATCGCCAACTATCAGGCAGTGGTGCCATCAACCTGGAACTCCGG
CCCGCGCAACGGACAGGATGAGCCGGGACCGTACGAGCAGGCGCTGGTTGGTACGCCGGTAGCAGACATCAACAAACCGC
TGGAGGTGGTGCGTACCGTGCACTCCTTCGACCCCTGTATGTCCTGCGCTGTGCACGTGGTGAATACCCTGGACGGCGAA
ACCACCAAGGTAAAAATCCTGTAA

Upstream 100 bases:

>100_bases
AAGTCGTTGCTTATTTGCTGCTGATCCGGTTGCTGCCGGTGTTGCCAGCGCTTGACCATTTAAAACACCAAGGTTATCAG
GCCCAAGGGAAAGCTAAAGT

Downstream 100 bases:

>100_bases
TGAATACGCTGATTCTCGGGATTGGAAACCTGCTGCTCAGCGATGAGGCTGTCGGCGTGCGTATTGTTGAGCGCCTACAG
CGCGACTACCGCTTTCCGGC

Product: hydrogenase 2 large subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 large subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSQRITIDPITRIEGHLRIDCEIENGKVTKAWSSGTMWRGMEDIVKGNDPRDAWMIVQRICGVCTTIHAIASVRAVESAV
GAKVPVNAQYIRNLILSAHSIHDHIVHFYQLSAMDWVDITSALKADPNKCEAMLQGVSAWQLNSAGEFAKVQQKIRNLVD
SGQLGIFANGYWGHKEMKLSPEVNLIAVAHYLQALECQRDANRVVAILGGKSPHIQNLAVGGVANPINLNAPSVLNLERL
MYVKMFIERLGDFIEQVYKVDAAIIAANYPEWMTLGRGAQHYLSVPELPTDGDGGSFLLPGGYIENGDMANYRPINSQQD
PWLIAGIAESSKHSWYQDDANLKPWEGKTEPNYTGWQEDGKYSWVKSPTLYGKVVEVGPLADLLVKLAAKHEDTVKHYNE
VNGLYQKLTGKTLQVEQLHSTMGRIIGRAVRCCVLKDTLMMQWQALIDNIGKGDHVAYIPAEIDPNKEYRGVGFEEAPRG
ALSHWIVIKGGKIANYQAVVPSTWNSGPRNGQDEPGPYEQALVGTPVADINKPLEVVRTVHSFDPCMSCAVHVVNTLDGE
TTKVKIL

Sequences:

>Translated_567_residues
MSQRITIDPITRIEGHLRIDCEIENGKVTKAWSSGTMWRGMEDIVKGNDPRDAWMIVQRICGVCTTIHAIASVRAVESAV
GAKVPVNAQYIRNLILSAHSIHDHIVHFYQLSAMDWVDITSALKADPNKCEAMLQGVSAWQLNSAGEFAKVQQKIRNLVD
SGQLGIFANGYWGHKEMKLSPEVNLIAVAHYLQALECQRDANRVVAILGGKSPHIQNLAVGGVANPINLNAPSVLNLERL
MYVKMFIERLGDFIEQVYKVDAAIIAANYPEWMTLGRGAQHYLSVPELPTDGDGGSFLLPGGYIENGDMANYRPINSQQD
PWLIAGIAESSKHSWYQDDANLKPWEGKTEPNYTGWQEDGKYSWVKSPTLYGKVVEVGPLADLLVKLAAKHEDTVKHYNE
VNGLYQKLTGKTLQVEQLHSTMGRIIGRAVRCCVLKDTLMMQWQALIDNIGKGDHVAYIPAEIDPNKEYRGVGFEEAPRG
ALSHWIVIKGGKIANYQAVVPSTWNSGPRNGQDEPGPYEQALVGTPVADINKPLEVVRTVHSFDPCMSCAVHVVNTLDGE
TTKVKIL
>Mature_566_residues
SQRITIDPITRIEGHLRIDCEIENGKVTKAWSSGTMWRGMEDIVKGNDPRDAWMIVQRICGVCTTIHAIASVRAVESAVG
AKVPVNAQYIRNLILSAHSIHDHIVHFYQLSAMDWVDITSALKADPNKCEAMLQGVSAWQLNSAGEFAKVQQKIRNLVDS
GQLGIFANGYWGHKEMKLSPEVNLIAVAHYLQALECQRDANRVVAILGGKSPHIQNLAVGGVANPINLNAPSVLNLERLM
YVKMFIERLGDFIEQVYKVDAAIIAANYPEWMTLGRGAQHYLSVPELPTDGDGGSFLLPGGYIENGDMANYRPINSQQDP
WLIAGIAESSKHSWYQDDANLKPWEGKTEPNYTGWQEDGKYSWVKSPTLYGKVVEVGPLADLLVKLAAKHEDTVKHYNEV
NGLYQKLTGKTLQVEQLHSTMGRIIGRAVRCCVLKDTLMMQWQALIDNIGKGDHVAYIPAEIDPNKEYRGVGFEEAPRGA
LSHWIVIKGGKIANYQAVVPSTWNSGPRNGQDEPGPYEQALVGTPVADINKPLEVVRTVHSFDPCMSCAVHVVNTLDGET
TKVKIL

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=567, Percent_Identity=73.36860670194, Blast_Score=886, Evalue=0.0,
Organism=Escherichia coli, GI1787207, Length=591, Percent_Identity=41.7935702199662, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 62690; Mature: 62559

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRITIDPITRIEGHLRIDCEIENGKVTKAWSSGTMWRGMEDIVKGNDPRDAWMIVQRI
CCCCEEECCHHHCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHH
CGVCTTIHAIASVRAVESAVGAKVPVNAQYIRNLILSAHSIHDHIVHFYQLSAMDWVDIT
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SALKADPNKCEAMLQGVSAWQLNSAGEFAKVQQKIRNLVDSGQLGIFANGYWGHKEMKLS
HHHCCCHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEC
PEVNLIAVAHYLQALECQRDANRVVAILGGKSPHIQNLAVGGVANPINLNAPSVLNLERL
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
MYVKMFIERLGDFIEQVYKVDAAIIAANYPEWMTLGRGAQHYLSVPELPTDGDGGSFLLP
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCHHHCCCCCCCCCCCCCCEEEC
GGYIENGDMANYRPINSQQDPWLIAGIAESSKHSWYQDDANLKPWEGKTEPNYTGWQEDG
CCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTLYGKVVEVGPLADLLVKLAAKHEDTVKHYNEVNGLYQKLTGKTLQVEQLHS
CEEEECCCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
TMGRIIGRAVRCCVLKDTLMMQWQALIDNIGKGDHVAYIPAEIDPNKEYRGVGFEEAPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
ALSHWIVIKGGKIANYQAVVPSTWNSGPRNGQDEPGPYEQALVGTPVADINKPLEVVRTV
CCCEEEEEECCEEECEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCHHHHHHHHH
HSFDPCMSCAVHVVNTLDGETTKVKIL
HHCCHHHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure 
SQRITIDPITRIEGHLRIDCEIENGKVTKAWSSGTMWRGMEDIVKGNDPRDAWMIVQRI
CCCEEECCHHHCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHH
CGVCTTIHAIASVRAVESAVGAKVPVNAQYIRNLILSAHSIHDHIVHFYQLSAMDWVDIT
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SALKADPNKCEAMLQGVSAWQLNSAGEFAKVQQKIRNLVDSGQLGIFANGYWGHKEMKLS
HHHCCCHHHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEC
PEVNLIAVAHYLQALECQRDANRVVAILGGKSPHIQNLAVGGVANPINLNAPSVLNLERL
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHH
MYVKMFIERLGDFIEQVYKVDAAIIAANYPEWMTLGRGAQHYLSVPELPTDGDGGSFLLP
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCHHHCCCCCCCCCCCCCCEEEC
GGYIENGDMANYRPINSQQDPWLIAGIAESSKHSWYQDDANLKPWEGKTEPNYTGWQEDG
CCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KYSWVKSPTLYGKVVEVGPLADLLVKLAAKHEDTVKHYNEVNGLYQKLTGKTLQVEQLHS
CEEEECCCCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHH
TMGRIIGRAVRCCVLKDTLMMQWQALIDNIGKGDHVAYIPAEIDPNKEYRGVGFEEAPRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
ALSHWIVIKGGKIANYQAVVPSTWNSGPRNGQDEPGPYEQALVGTPVADINKPLEVVRTV
CCCEEEEEECCEEECEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCHHHCCHHHHHHHHH
HSFDPCMSCAVHVVNTLDGETTKVKIL
HHCCHHHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]