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The map label for this gene is hybD [H]

Identifier: 238920544

GI number: 238920544

Start: 2550216

End: 2550710

Strand: Reverse

Name: hybD [H]

Synonym: NT01EI_2656

Alternate gene names: 238920544

Gene position: 2550710-2550216 (Counterclockwise)

Preceding gene: 238920545

Following gene: 238920543

Centisome position: 66.91

GC content: 60.61

Gene sequence:

>495_bases
ATGAATACGCTGATTCTCGGGATTGGAAACCTGCTGCTCAGCGATGAGGCTGTCGGCGTGCGTATTGTTGAGCGCCTACA
GCGCGACTACCGCTTTCCGGCCAATGTCGAGCTGATGGATGGCGGTACCGCAGGAATGGAGCTGCTGGAGTACCTGGCCT
GCCGCGATCACCTGATCGTGGTGGATGCCGTGTTCTGCGGTGGCGAGCCTGGCAGTATCATCACGCTGCGTGATGAGGAA
GTCCCGGCGATGTTCAGCCGCAAGATTTCGCCTCATCAGCTGGGGCTGTCCGATGTACTGTCGGCACTGCGCCTGACGGA
CGAGTTTCCGAAGAAGCTGACGCTGGTCGGCATTGAGCCGCAGTCGCTGGAGCCGAACATTGGTTTGACGCCAGCGATCG
CCAGCGCAGTGCCGGTGGCGATGGATAGGGTGTTGGCTATCCTGCGTGAGCAGGGGATCCAGGCCCTGCATCAGGGGGAC
ATCCATGCACGTTGA

Upstream 100 bases:

>100_bases
AGGTGGTGCGTACCGTGCACTCCTTCGACCCCTGTATGTCCTGCGCTGTGCACGTGGTGAATACCCTGGACGGCGAAACC
ACCAAGGTAAAAATCCTGTA

Downstream 100 bases:

>100_bases
GTTCCCGCACGATGAGCAGCCGGAGCAGGGCATGGTCGCCGGGCGCTTAGGGTTCGACAGCGATCCTGCCGCGCAGCTGA
TCGCACAGTATCAGCAGATC

Product: hydrogenase 2 maturation endopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNTLILGIGNLLLSDEAVGVRIVERLQRDYRFPANVELMDGGTAGMELLEYLACRDHLIVVDAVFCGGEPGSIITLRDEE
VPAMFSRKISPHQLGLSDVLSALRLTDEFPKKLTLVGIEPQSLEPNIGLTPAIASAVPVAMDRVLAILREQGIQALHQGD
IHAR

Sequences:

>Translated_164_residues
MNTLILGIGNLLLSDEAVGVRIVERLQRDYRFPANVELMDGGTAGMELLEYLACRDHLIVVDAVFCGGEPGSIITLRDEE
VPAMFSRKISPHQLGLSDVLSALRLTDEFPKKLTLVGIEPQSLEPNIGLTPAIASAVPVAMDRVLAILREQGIQALHQGD
IHAR
>Mature_164_residues
MNTLILGIGNLLLSDEAVGVRIVERLQRDYRFPANVELMDGGTAGMELLEYLACRDHLIVVDAVFCGGEPGSIITLRDEE
VPAMFSRKISPHQLGLSDVLSALRLTDEFPKKLTLVGIEPQSLEPNIGLTPAIASAVPVAMDRVLAILREQGIQALHQGD
IHAR

Specific function: Protease involved in the C-terminal processing of hybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of hybC [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=163, Percent_Identity=60.7361963190184, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI1787209, Length=122, Percent_Identity=41.8032786885246, Blast_Score=105, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 17819; Mature: 17819

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLILGIGNLLLSDEAVGVRIVERLQRDYRFPANVELMDGGTAGMELLEYLACRDHLIV
CCEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCEEE
VDAVFCGGEPGSIITLRDEEVPAMFSRKISPHQLGLSDVLSALRLTDEFPKKLTLVGIEP
EEEEEECCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCC
QSLEPNIGLTPAIASAVPVAMDRVLAILREQGIQALHQGDIHAR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNTLILGIGNLLLSDEAVGVRIVERLQRDYRFPANVELMDGGTAGMELLEYLACRDHLIV
CCEEEEECCHHHHCCCCHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCEEE
VDAVFCGGEPGSIITLRDEEVPAMFSRKISPHQLGLSDVLSALRLTDEFPKKLTLVGIEP
EEEEEECCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCC
QSLEPNIGLTPAIASAVPVAMDRVLAILREQGIQALHQGDIHAR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503; 10331925 [H]