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The map label for this gene is rsuA [H]

Identifier: 238920493

GI number: 238920493

Start: 2495629

End: 2496336

Strand: Reverse

Name: rsuA [H]

Synonym: NT01EI_2604

Alternate gene names: 238920493

Gene position: 2496336-2495629 (Counterclockwise)

Preceding gene: 238920498

Following gene: 238920492

Centisome position: 65.48

GC content: 62.85

Gene sequence:

>708_bases
ATGAGACTGGACAAATTTATTTCCCAGCAGCTGGGGATCAGCCGCTCATTGGTGATCCGCGAGCTGCGGGCTGCGCGCGT
CACCGTCGATGGTGTCGTCGCCCGCAGCGGATCGCTGCAGGTCACGCCGGATCAGGAGGTTGCCTATGAGGGCAACGTGC
TGACGTTTATCACCGGGCCGCGCTACTTTATGCTGCATAAGCCCGAGGGTTACGTCTGCTCGACGGACGATCCGGACCAT
GCCACCATTCTCTACTTTATCGATGAGCCGATGGCAGAAAAGCTGCACGCCGCCGGGCGTCTGGACCTGGACACCACCGG
TCTGGTGCTGCTGACGGACGACGGGCAGTGGTCTCACCGCATCACTTCGCCGCGCCATCACTGTGAGAAGAGCTATCTGG
TGACGCTGGAGCAGCCGCTGGCGCCGCAGACCGCGGCGCTGTTTGCCCAGGGGATTATGTTGAACGGTGAAACGGATCTG
ACCAAACCGGCGCAGCTGGAGGCTTTCGACGAGCATCATGTCCGCCTGACCATCAGCGAAGGGCGCTATCATCAGGTGAA
ACGCATGTTCGCCGCCGTGGGTAACCGGGTGGTGGGACTGCACCGCGAGCGTATCGGTGCCATTGCGCTGGATGCGGCGC
TGGCGCCCGGCGAGTACCGTGCGCTGAGCGACGATGAGGTGGCCAGCGCACTGGCTGGCAGGGGGTAA

Upstream 100 bases:

>100_bases
GGTCTGCGTTTGCCTCGGCAGTGGGAGATTCCCGCGTTCGGTACAGTGCCGCGGACAATATCGGTTTTTATTTCGCCTTG
GTTATAACAGAGCAGGTTTC

Downstream 100 bases:

>100_bases
GGAGCCCGGCGTGTAAAGAGGGCCTCATCTGAGGCTTCTCCTGATATTAGGAGTGCTGCCGATGTTGATGCCGCTGGTCA
TCGGCATCTATCTGCCCAGT

Product: 16S rRNA pseudouridylate synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRLDKFISQQLGISRSLVIRELRAARVTVDGVVARSGSLQVTPDQEVAYEGNVLTFITGPRYFMLHKPEGYVCSTDDPDH
ATILYFIDEPMAEKLHAAGRLDLDTTGLVLLTDDGQWSHRITSPRHHCEKSYLVTLEQPLAPQTAALFAQGIMLNGETDL
TKPAQLEAFDEHHVRLTISEGRYHQVKRMFAAVGNRVVGLHRERIGAIALDAALAPGEYRALSDDEVASALAGRG

Sequences:

>Translated_235_residues
MRLDKFISQQLGISRSLVIRELRAARVTVDGVVARSGSLQVTPDQEVAYEGNVLTFITGPRYFMLHKPEGYVCSTDDPDH
ATILYFIDEPMAEKLHAAGRLDLDTTGLVLLTDDGQWSHRITSPRHHCEKSYLVTLEQPLAPQTAALFAQGIMLNGETDL
TKPAQLEAFDEHHVRLTISEGRYHQVKRMFAAVGNRVVGLHRERIGAIALDAALAPGEYRALSDDEVASALAGRG
>Mature_235_residues
MRLDKFISQQLGISRSLVIRELRAARVTVDGVVARSGSLQVTPDQEVAYEGNVLTFITGPRYFMLHKPEGYVCSTDDPDH
ATILYFIDEPMAEKLHAAGRLDLDTTGLVLLTDDGQWSHRITSPRHHCEKSYLVTLEQPLAPQTAALFAQGIMLNGETDL
TKPAQLEAFDEHHVRLTISEGRYHQVKRMFAAVGNRVVGLHRERIGAIALDAALAPGEYRALSDDEVASALAGRG

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=70.1298701298701, Blast_Score=345, Evalue=1e-96,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=30.0429184549356, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787524, Length=229, Percent_Identity=27.9475982532751, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 25915; Mature: 25915

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKFISQQLGISRSLVIRELRAARVTVDGVVARSGSLQVTPDQEVAYEGNVLTFITGP
CCHHHHHHHHHCCHHHHHHHHHHHHEEEEEHEEECCCCEEECCCCCEEECCCEEEEEECC
RYFMLHKPEGYVCSTDDPDHATILYFIDEPMAEKLHAAGRLDLDTTGLVLLTDDGQWSHR
EEEEEECCCCCEECCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
ITSPRHHCEKSYLVTLEQPLAPQTAALFAQGIMLNGETDLTKPAQLEAFDEHHVRLTISE
CCCCHHHCCCEEEEEECCCCCCHHHHHHHHCEEECCCCCCCCCCEEEECCCCEEEEEECC
GRYHQVKRMFAAVGNRVVGLHRERIGAIALDAALAPGEYRALSDDEVASALAGRG
CCHHHHHHHHHHHCCEEEEEHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRLDKFISQQLGISRSLVIRELRAARVTVDGVVARSGSLQVTPDQEVAYEGNVLTFITGP
CCHHHHHHHHHCCHHHHHHHHHHHHEEEEEHEEECCCCEEECCCCCEEECCCEEEEEECC
RYFMLHKPEGYVCSTDDPDHATILYFIDEPMAEKLHAAGRLDLDTTGLVLLTDDGQWSHR
EEEEEECCCCCEECCCCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
ITSPRHHCEKSYLVTLEQPLAPQTAALFAQGIMLNGETDLTKPAQLEAFDEHHVRLTISE
CCCCHHHCCCEEEEEECCCCCCHHHHHHHHCEEECCCCCCCCCCEEEECCCCEEEEEECC
GRYHQVKRMFAAVGNRVVGLHRERIGAIALDAALAPGEYRALSDDEVASALAGRG
CCHHHHHHHHHHHCCEEEEEHHHHHCEEEEEEEECCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]