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The map label for this gene is artJ [H]

Identifier: 238920412

GI number: 238920412

Start: 2423646

End: 2424380

Strand: Direct

Name: artJ [H]

Synonym: NT01EI_2522

Alternate gene names: 238920412

Gene position: 2423646-2424380 (Clockwise)

Preceding gene: 238920411

Following gene: 238920413

Centisome position: 63.57

GC content: 58.37

Gene sequence:

>735_bases
ATGAAGAAGTTACTGGCCTGCGCCATCGTTGTCGCCTGCAGTTTCAGCGCCCAGGCGGCAGAGAATATCCGCTTCGCCTC
ATCGGCCACCTACCCGCCGTTCGAATCCCTGGATGCCAGCAACAACATCGTCGGCTTCGACATCGATCTGGCCAAGGCGC
TGTGCCAGGAGATGAAGGCGAACTGCACCTTCAACAACCAGGCGTTTGACAGCCTGATCACGGCGCTGAAGTTCAAACGC
TACGACGCCGTTATCTCCGGCATGGACATCACCCCGGAGCGACAGAGGCAGGTGGCGTTCACCCAGCCTTACTACGCCAA
CTCGGCGATCATCATCGCGCAGAAAGGCAAATATCACTCGATAGCCGATATGAAGGGGCTGCGGGTCGGCATGGAAAACG
GGACCACTCACCAGAAGTACCTGCAGGATAAACATCCGGAGATCAAGCCGGTGGCCTATGACAGCTACCAGAATGCGATT
CTGGATCTGAAAAACGGGCGTATTGATGGCGTCTTCGGCGATACCGCGGTGGTCAATGAGTGGCTGAAAAACAATCCGCA
GCTGGCGGCGGTCGGCGAGTATGTCACCGATCCGCAGTACTTTGGCATCGGGCTGGGCATCGCGGTGCGCCCGGACAACC
AGGCGCTGCTACATCAGCTGAATCAGGCGCTGGCCGCCATCAAGGCGAACGGCACCTATCAGAAGATCAGCGACAAGTGG
TTCCCGGCGTCCTGA

Upstream 100 bases:

>100_bases
GTGCCATCGCGCCGATCGTTTCCGGCACGCGCCCAAGGCAGAGGGGAATCGACAGACGCCCTCTATCCTTATCGGGATTA
GCCTTGTACAGGAGTTAAGC

Downstream 100 bases:

>100_bases
ATGCATCCGGTTGGCGCTCCGGGCGCGATTTGTTAGCATGTCGCCTCCGCCCGTCGGCGCCACGCCACGCTTCAGGATAC
GCTATGGACTATGACCACTA

Product: arginine-binding periplasmic protein 2

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKKLLACAIVVACSFSAQAAENIRFASSATYPPFESLDASNNIVGFDIDLAKALCQEMKANCTFNNQAFDSLITALKFKR
YDAVISGMDITPERQRQVAFTQPYYANSAIIIAQKGKYHSIADMKGLRVGMENGTTHQKYLQDKHPEIKPVAYDSYQNAI
LDLKNGRIDGVFGDTAVVNEWLKNNPQLAAVGEYVTDPQYFGIGLGIAVRPDNQALLHQLNQALAAIKANGTYQKISDKW
FPAS

Sequences:

>Translated_244_residues
MKKLLACAIVVACSFSAQAAENIRFASSATYPPFESLDASNNIVGFDIDLAKALCQEMKANCTFNNQAFDSLITALKFKR
YDAVISGMDITPERQRQVAFTQPYYANSAIIIAQKGKYHSIADMKGLRVGMENGTTHQKYLQDKHPEIKPVAYDSYQNAI
LDLKNGRIDGVFGDTAVVNEWLKNNPQLAAVGEYVTDPQYFGIGLGIAVRPDNQALLHQLNQALAAIKANGTYQKISDKW
FPAS
>Mature_244_residues
MKKLLACAIVVACSFSAQAAENIRFASSATYPPFESLDASNNIVGFDIDLAKALCQEMKANCTFNNQAFDSLITALKFKR
YDAVISGMDITPERQRQVAFTQPYYANSAIIIAQKGKYHSIADMKGLRVGMENGTTHQKYLQDKHPEIKPVAYDSYQNAI
LDLKNGRIDGVFGDTAVVNEWLKNNPQLAAVGEYVTDPQYFGIGLGIAVRPDNQALLHQLNQALAAIKANGTYQKISDKW
FPAS

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=242, Percent_Identity=73.1404958677686, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1787088, Length=241, Percent_Identity=65.5601659751037, Blast_Score=347, Evalue=5e-97,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=37.9912663755458, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI1788649, Length=251, Percent_Identity=35.0597609561753, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788228, Length=254, Percent_Identity=28.3464566929134, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=29.5081967213115, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26879; Mature: 26879

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLACAIVVACSFSAQAAENIRFASSATYPPFESLDASNNIVGFDIDLAKALCQEMKA
CHHHHHHHHHHHHCCCHHHHHCEEECCCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHC
NCTFNNQAFDSLITALKFKRYDAVISGMDITPERQRQVAFTQPYYANSAIIIAQKGKYHS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEECCCEECCCEEEEEECCCCCC
IADMKGLRVGMENGTTHQKYLQDKHPEIKPVAYDSYQNAILDLKNGRIDGVFGDTAVVNE
HHCCCCEEEECCCCCCHHHHHHHCCCCCCCEEECCHHHHEEECCCCEECCEECCHHHHHH
WLKNNPQLAAVGEYVTDPQYFGIGLGIAVRPDNQALLHQLNQALAAIKANGTYQKISDKW
HHHCCCCEEECCCHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
FPAS
CCCC
>Mature Secondary Structure
MKKLLACAIVVACSFSAQAAENIRFASSATYPPFESLDASNNIVGFDIDLAKALCQEMKA
CHHHHHHHHHHHHCCCHHHHHCEEECCCCCCCCHHHCCCCCCEEEECHHHHHHHHHHHHC
NCTFNNQAFDSLITALKFKRYDAVISGMDITPERQRQVAFTQPYYANSAIIIAQKGKYHS
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCEEECCCEECCCEEEEEECCCCCC
IADMKGLRVGMENGTTHQKYLQDKHPEIKPVAYDSYQNAILDLKNGRIDGVFGDTAVVNE
HHCCCCEEEECCCCCCHHHHHHHCCCCCCCEEECCHHHHEEECCCCEECCEECCHHHHHH
WLKNNPQLAAVGEYVTDPQYFGIGLGIAVRPDNQALLHQLNQALAAIKANGTYQKISDKW
HHHCCCCEEECCCHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHCCCC
FPAS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]