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The map label for this gene is ansB [H]

Identifier: 238920350

GI number: 238920350

Start: 2352631

End: 2353680

Strand: Reverse

Name: ansB [H]

Synonym: NT01EI_2460

Alternate gene names: 238920350

Gene position: 2353680-2352631 (Counterclockwise)

Preceding gene: 238920355

Following gene: 238920349

Centisome position: 61.74

GC content: 59.05

Gene sequence:

>1050_bases
ATGAAGTTAGTCAAGGTTAGCCTGTTGGCATTATTGGTTTCCGGCTTTAGCGGCGCGGCGCTGGCGCTGCCGAGCATCAC
CATACTGGCGACGGGCGGTACCATTGCCGGCGGCGGCGACTCCGCCACCAAGTCCAACTACACCGCCGGGAAGGTTGGGG
TAGAGGCACTGGTGGACGCGGTGCCGCAGCTGAAAAACATTGCCAACATTAAGGGTGAGCAGCTGGTGAATATTGGCTCC
CAGGATATGAATGATCAGGTCTGGCTGAAGCTGGCGAAGAAAATCAATGTCGAGTGCAGTAAGACCGACGGGTTTGTGAT
CACCCACGGCACCGATACGATGGAAGAGACGGCCTATTTCCTGGATCTGACCGTGAAGTGCGACAAACCGGTAGTGTTGG
TCGGCGCGATGCGCCCGTCCACGGCGATGAGTGCAGACGGTCCGTTCAACCTGTATAACGCGGTCGTTACCGCTGCCGAC
CCGGCCTCCGCCAACCGTGGCGTACTGGTTGCGATGAATGACACGGTGCTGGATGGTCGCGACGTGACCAAGACCAACAC
CACCGGGGTGGAAACCTTTAAGTCCGTCAATTATGGTCCGCTGGGCTATATCCACAACGGCAAGATCGACTATCAGCGCA
CACCGGCGCGTAAGCACACGACCCAGACGCCGTTTGACGTCAGTAAGTTGACCTCGCTGCCGAAAGTCGGCATCGTCTAC
AACTATGCCAACGCCAGTGCGCTACCGGTTGAGGCGCTGGTGAAAGACGGCTACCAGGGGATCGTGAGCGCCGGCGTCGG
CAACGGCAACATGTATAAGACGGTATTCGACGCGTTGGCCAATGCCGCGCACGACGGCGTTGCGGTGGTTCGCTCCTCCC
GCGTGCCGACAGGCTCAACCACGCAGGACGCCGAAGTGGACGACGCCAGGTACGGCTTTATCGCCTCCGGTACGCTGAAT
CCGCAGAAGGCCCGTATCCTGCTGCAGCTGGCGCTGACGGAGACCAAAAATCCGCAGCAGATCCAGAAAATCTTCAACAC
CTACGGATAG

Upstream 100 bases:

>100_bases
TTTAACCTATTCCTCCACGTATTCACTATATCTGTCTGAGGTCACATTTTTTATATGGATGGAATAGATAATTCATACAG
TTAATATAAGGAGAATTGGG

Downstream 100 bases:

>100_bases
CGGACGGGGGCATACGTCCCCCGTTTCCCGCCGACCACCCGATGGCGTCCTAGGGCGCCATCCTGCATCTTTCTCCGTCG
AATCTTCCGTGTTGGCGACG

Product: L-asparaginase II

Products: NA

Alternate protein names: L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MKLVKVSLLALLVSGFSGAALALPSITILATGGTIAGGGDSATKSNYTAGKVGVEALVDAVPQLKNIANIKGEQLVNIGS
QDMNDQVWLKLAKKINVECSKTDGFVITHGTDTMEETAYFLDLTVKCDKPVVLVGAMRPSTAMSADGPFNLYNAVVTAAD
PASANRGVLVAMNDTVLDGRDVTKTNTTGVETFKSVNYGPLGYIHNGKIDYQRTPARKHTTQTPFDVSKLTSLPKVGIVY
NYANASALPVEALVKDGYQGIVSAGVGNGNMYKTVFDALANAAHDGVAVVRSSRVPTGSTTQDAEVDDARYGFIASGTLN
PQKARILLQLALTETKNPQQIQKIFNTYG

Sequences:

>Translated_349_residues
MKLVKVSLLALLVSGFSGAALALPSITILATGGTIAGGGDSATKSNYTAGKVGVEALVDAVPQLKNIANIKGEQLVNIGS
QDMNDQVWLKLAKKINVECSKTDGFVITHGTDTMEETAYFLDLTVKCDKPVVLVGAMRPSTAMSADGPFNLYNAVVTAAD
PASANRGVLVAMNDTVLDGRDVTKTNTTGVETFKSVNYGPLGYIHNGKIDYQRTPARKHTTQTPFDVSKLTSLPKVGIVY
NYANASALPVEALVKDGYQGIVSAGVGNGNMYKTVFDALANAAHDGVAVVRSSRVPTGSTTQDAEVDDARYGFIASGTLN
PQKARILLQLALTETKNPQQIQKIFNTYG
>Mature_349_residues
MKLVKVSLLALLVSGFSGAALALPSITILATGGTIAGGGDSATKSNYTAGKVGVEALVDAVPQLKNIANIKGEQLVNIGS
QDMNDQVWLKLAKKINVECSKTDGFVITHGTDTMEETAYFLDLTVKCDKPVVLVGAMRPSTAMSADGPFNLYNAVVTAAD
PASANRGVLVAMNDTVLDGRDVTKTNTTGVETFKSVNYGPLGYIHNGKIDYQRTPARKHTTQTPFDVSKLTSLPKVGIVY
NYANASALPVEALVKDGYQGIVSAGVGNGNMYKTVFDALANAAHDGVAVVRSSRVPTGSTTQDAEVDDARYGFIASGTLN
PQKARILLQLALTETKNPQQIQKIFNTYG

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=228, Percent_Identity=28.5087719298246, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789327, Length=348, Percent_Identity=85.3448275862069, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1788065, Length=225, Percent_Identity=31.5555555555556, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17558278, Length=226, Percent_Identity=28.7610619469027, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=226, Percent_Identity=28.7610619469027, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=226, Percent_Identity=28.7610619469027, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=226, Percent_Identity=28.7610619469027, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=369, Percent_Identity=40.9214092140921, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323187, Length=369, Percent_Identity=40.9214092140921, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323186, Length=369, Percent_Identity=40.9214092140921, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323184, Length=369, Percent_Identity=40.9214092140921, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6320527, Length=351, Percent_Identity=38.4615384615385, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24645530, Length=216, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639609, Length=233, Percent_Identity=26.6094420600858, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36792; Mature: 36792

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVKVSLLALLVSGFSGAALALPSITILATGGTIAGGGDSATKSNYTAGKVGVEALVDA
CCHHHHHHHHHHHHCCCCCEEECCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHH
VPQLKNIANIKGEQLVNIGSQDMNDQVWLKLAKKINVECSKTDGFVITHGTDTMEETAYF
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCCHHHCEEE
LDLTVKCDKPVVLVGAMRPSTAMSADGPFNLYNAVVTAADPASANRGVLVAMNDTVLDGR
EEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHEEEEECCCCCCCCCEEEEECCEEECCC
DVTKTNTTGVETFKSVNYGPLGYIHNGKIDYQRTPARKHTTQTPFDVSKLTSLPKVGIVY
CCCCCCCCCHHHHHCCCCCCCEEEECCEECCCCCCCCCCCCCCCCCHHHHHCCCCCEEEE
NYANASALPVEALVKDGYQGIVSAGVGNGNMYKTVFDALANAAHDGVAVVRSSRVPTGST
EECCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
TQDAEVDDARYGFIASGTLNPQKARILLQLALTETKNPQQIQKIFNTYG
CCCCCCCCCCCCEEEECCCCHHHHEEEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLVKVSLLALLVSGFSGAALALPSITILATGGTIAGGGDSATKSNYTAGKVGVEALVDA
CCHHHHHHHHHHHHCCCCCEEECCCEEEEEECCEEECCCCCCCCCCCCCHHHHHHHHHHH
VPQLKNIANIKGEQLVNIGSQDMNDQVWLKLAKKINVECSKTDGFVITHGTDTMEETAYF
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCCHHHCEEE
LDLTVKCDKPVVLVGAMRPSTAMSADGPFNLYNAVVTAADPASANRGVLVAMNDTVLDGR
EEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHEEEEECCCCCCCCCEEEEECCEEECCC
DVTKTNTTGVETFKSVNYGPLGYIHNGKIDYQRTPARKHTTQTPFDVSKLTSLPKVGIVY
CCCCCCCCCHHHHHCCCCCCCEEEECCEECCCCCCCCCCCCCCCCCHHHHHCCCCCEEEE
NYANASALPVEALVKDGYQGIVSAGVGNGNMYKTVFDALANAAHDGVAVVRSSRVPTGST
EECCCCCCCHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
TQDAEVDDARYGFIASGTLNPQKARILLQLALTETKNPQQIQKIFNTYG
CCCCCCCCCCCCEEEECCCCHHHHEEEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2407723; 2144836; 9278503; 6766894; 387570; 321449; 4561256; 1906013; 1521538; 1287659; 8434007; 8706862 [H]