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The map label for this gene is tycC [H]

Identifier: 238920284

GI number: 238920284

Start: 2292332

End: 2294572

Strand: Reverse

Name: tycC [H]

Synonym: NT01EI_2394

Alternate gene names: 238920284

Gene position: 2294572-2292332 (Counterclockwise)

Preceding gene: 238920286

Following gene: 238920283

Centisome position: 60.19

GC content: 47.39

Gene sequence:

>2241_bases
ATGACTATGTTAGATAGAACATTAATTTCAGCATTTACAATTCATGTTAAAAATACACCAGATAAAGTGGCGATAAAACA
CAATGGAAAGCAAACAACCTATAGGCAGCTTGATGAATTATCAACAAATATTGCCAGTTATATCTGGCAAAGAAAACCTG
GTCATGATAAGTCCACGTCAATATTAATTTGCCTCGAGCGCAGCGAACTTACTATTGGCGTGATTCTTGCTTGCGTGAAG
CTTGGTTTTACATATGTTCCCTTTGATCCAAAAAATCCTGACGAACGCTTGCAATATATGCTTGATGACTGTCGGCCCGC
ATTGGTTATCACCAGCAAGAAAGATGCAAAAAGGCAAGATGGTTACCTTGAAATATATGTTGAGGACATCCCGCTGCCCA
CAGCGGAAGTATCTCCTGATTTTCCAGGAGTATCGAATCTGGAGGCGGTTTATATTATGTACACCTCCGGCTCGACCGGA
AATCCCAAGGGTGTCGTCATTTCACAGAAAGGAATCCTTCGTCTGGTGCTTGATGCCAAACCATTCCAGTTTTCATCTGA
TGCCATTATTGCGCAATCTGGGAATATCGCTTTTGATGCGTCAACGTTTGAAATCTGGGGGGCGCTGCTTAACGGTGCTA
CGCTGGTTATTACGCCTTATGAGACCGTGATTGATAGTGATGCATTGGCGACGTTTCTGTGCGTCGAAACAATCACTGAT
GCTTGGTTTACCGTGGCACTCTTTAACCTGCTGGTGATGGAAAACCCTGGTGTGTTTGGTTCGTTAAACAATGTCTTAAT
CGGTGGGGATGCGCTAAACCCCAAATTGGTCCATGCCGTACTCTGTTCTGATACACCGCCTGGTGCACTGTGGAATGGCT
ATGGTCCGACAGAAAATACGGTATTTACTACGTTGCACCGAATTACGCTGGAAGACTCAACGCGGAGTAGTATTCCAATT
GGAATACCGATAGCGGGAACGCACTGCTATGTGCTCGATGCGGATCGTCAGCCTGTTATACCGGGAGAGGAGGGGGAGCT
ATATACCAGTGGGGCCGGGTTGGCGACCGGATATCTGAACAAGCCGGAAAAAACCGCTGAGGCATTTTTCCCTAATCCTT
ACTATAAGCTAGAGAAATGCCAGAACCTGCAATCCGCAAGCGCATGGATGTATAAAACCGGCGATCGCGTGAAGATGCTT
GCAGATGGCACTTTGGATTTTCTGGGGCGGGTGGATAACCAGGTAAAAATTCGCGGATTCCGTCTGGAACCTGGAGAGGT
GGAGTTTCAGCTGTGCCAACTCGCTGATGTGGATCTGGCTGTTGTGACGGTTGTGGAACATCAGGGGCAGAAAAAACTGG
CTGCATGGTGTAAAAGCTCCCGGAATGCTAACGAGATTCTGCAACTATTCCGCCAGCAGGTTCCGGCATATATGGTGCCT
TCTTATCTTCAGGTGGTAGAAAGCTTCCCCGTAACGGCAAACGGTAAAATTGACAAACGTAGTCTTCCTGCGCCTCAGTT
CAACAATCATCAGGCCACACCGCCGCAGACGGCCACAGAAATTTGGCTAAGTAAGACCTGGCAGCAGTTGCTCTCTTTAC
CACATCCCGTTGGCCGTGAGGATAACTTCTCTTCTCTTGGTGGGCATTCACTGCTGATTGTTAAACTCAAGCAAAAAATT
CAAACAGGCCTGAATAAACGTGTTTCATTAACTGAACTATTCAATCTTTCTTCGTTGAGCGACATGGCTGAGTATATCGA
TACGCTGCCAGAAAGCTGCGGCGTTATCACACTCAACCGGGTTGGTTACGGGGAAAAAATCGCGCTCACAAACGAACAGC
TTCGTTTGTGGTTGATTTGCAATCGTGAGCCACAATTACCCCACTACTCTATTCCCCTGGTGTTTCAACTTTCAGGTGAA
CTTCATCCCGAGCGGCTGGCGCAAGCGCTGGATTCGCTCTGCCAGCGCCATCAAAGCCTGCGCTCGCGTATTTTCAACAC
GGAAGGGACGCCTTGGCAGCAAGTGGCAGAAATGTCCCCCCAGCTGATTATGGATGAAGTAAAGGATGAAGATACGCTGC
AGGAGATACTGCGTCAGGAGGTTCAACGGCCGTTCACCTTTGGGAATGATCCATTACTGCGTATGGTGCTCTATCGCGTA
GCTCATAGCCGCTGGGTACTGTTTATTAATATTCACCATATTATTACAGATGGCTGGTCAATGGGCGTTTTTTTTCCGTG
A

Upstream 100 bases:

>100_bases
TTTATGCGGTGCGTCTGTTGAGCTCACCTGCAAGGCACTTGGCATTAAGTAAAATCAATGTCTTCATTGCTGTTGCATAC
CATAAGTGGATATTTTACAT

Downstream 100 bases:

>100_bases
GCTTTCATCCCTCTACGCTCAGGATGACGAACTTCCTGCACTGGATTACCAATTTATCGATTATTGTGCCTGGCAGCAGA
GGTTGGATCACTCGGCTGAT

Product: hypothetical protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: Tyrocidine synthase III; ATP-dependent asparagine adenylase; AsnA; Asparagine activase; ATP-dependent glutamine adenylase; GlnA; Glutamine activase; ATP-dependent tyrosine adenylase; TyrA; Tyrosine activase; ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent ornithine adenylase; OrnA; Ornithine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase [H]

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MTMLDRTLISAFTIHVKNTPDKVAIKHNGKQTTYRQLDELSTNIASYIWQRKPGHDKSTSILICLERSELTIGVILACVK
LGFTYVPFDPKNPDERLQYMLDDCRPALVITSKKDAKRQDGYLEIYVEDIPLPTAEVSPDFPGVSNLEAVYIMYTSGSTG
NPKGVVISQKGILRLVLDAKPFQFSSDAIIAQSGNIAFDASTFEIWGALLNGATLVITPYETVIDSDALATFLCVETITD
AWFTVALFNLLVMENPGVFGSLNNVLIGGDALNPKLVHAVLCSDTPPGALWNGYGPTENTVFTTLHRITLEDSTRSSIPI
GIPIAGTHCYVLDADRQPVIPGEEGELYTSGAGLATGYLNKPEKTAEAFFPNPYYKLEKCQNLQSASAWMYKTGDRVKML
ADGTLDFLGRVDNQVKIRGFRLEPGEVEFQLCQLADVDLAVVTVVEHQGQKKLAAWCKSSRNANEILQLFRQQVPAYMVP
SYLQVVESFPVTANGKIDKRSLPAPQFNNHQATPPQTATEIWLSKTWQQLLSLPHPVGREDNFSSLGGHSLLIVKLKQKI
QTGLNKRVSLTELFNLSSLSDMAEYIDTLPESCGVITLNRVGYGEKIALTNEQLRLWLICNREPQLPHYSIPLVFQLSGE
LHPERLAQALDSLCQRHQSLRSRIFNTEGTPWQQVAEMSPQLIMDEVKDEDTLQEILRQEVQRPFTFGNDPLLRMVLYRV
AHSRWVLFINIHHIITDGWSMGVFFP

Sequences:

>Translated_746_residues
MTMLDRTLISAFTIHVKNTPDKVAIKHNGKQTTYRQLDELSTNIASYIWQRKPGHDKSTSILICLERSELTIGVILACVK
LGFTYVPFDPKNPDERLQYMLDDCRPALVITSKKDAKRQDGYLEIYVEDIPLPTAEVSPDFPGVSNLEAVYIMYTSGSTG
NPKGVVISQKGILRLVLDAKPFQFSSDAIIAQSGNIAFDASTFEIWGALLNGATLVITPYETVIDSDALATFLCVETITD
AWFTVALFNLLVMENPGVFGSLNNVLIGGDALNPKLVHAVLCSDTPPGALWNGYGPTENTVFTTLHRITLEDSTRSSIPI
GIPIAGTHCYVLDADRQPVIPGEEGELYTSGAGLATGYLNKPEKTAEAFFPNPYYKLEKCQNLQSASAWMYKTGDRVKML
ADGTLDFLGRVDNQVKIRGFRLEPGEVEFQLCQLADVDLAVVTVVEHQGQKKLAAWCKSSRNANEILQLFRQQVPAYMVP
SYLQVVESFPVTANGKIDKRSLPAPQFNNHQATPPQTATEIWLSKTWQQLLSLPHPVGREDNFSSLGGHSLLIVKLKQKI
QTGLNKRVSLTELFNLSSLSDMAEYIDTLPESCGVITLNRVGYGEKIALTNEQLRLWLICNREPQLPHYSIPLVFQLSGE
LHPERLAQALDSLCQRHQSLRSRIFNTEGTPWQQVAEMSPQLIMDEVKDEDTLQEILRQEVQRPFTFGNDPLLRMVLYRV
AHSRWVLFINIHHIITDGWSMGVFFP
>Mature_745_residues
TMLDRTLISAFTIHVKNTPDKVAIKHNGKQTTYRQLDELSTNIASYIWQRKPGHDKSTSILICLERSELTIGVILACVKL
GFTYVPFDPKNPDERLQYMLDDCRPALVITSKKDAKRQDGYLEIYVEDIPLPTAEVSPDFPGVSNLEAVYIMYTSGSTGN
PKGVVISQKGILRLVLDAKPFQFSSDAIIAQSGNIAFDASTFEIWGALLNGATLVITPYETVIDSDALATFLCVETITDA
WFTVALFNLLVMENPGVFGSLNNVLIGGDALNPKLVHAVLCSDTPPGALWNGYGPTENTVFTTLHRITLEDSTRSSIPIG
IPIAGTHCYVLDADRQPVIPGEEGELYTSGAGLATGYLNKPEKTAEAFFPNPYYKLEKCQNLQSASAWMYKTGDRVKMLA
DGTLDFLGRVDNQVKIRGFRLEPGEVEFQLCQLADVDLAVVTVVEHQGQKKLAAWCKSSRNANEILQLFRQQVPAYMVPS
YLQVVESFPVTANGKIDKRSLPAPQFNNHQATPPQTATEIWLSKTWQQLLSLPHPVGREDNFSSLGGHSLLIVKLKQKIQ
TGLNKRVSLTELFNLSSLSDMAEYIDTLPESCGVITLNRVGYGEKIALTNEQLRLWLICNREPQLPHYSIPLVFQLSGEL
HPERLAQALDSLCQRHQSLRSRIFNTEGTPWQQVAEMSPQLIMDEVKDEDTLQEILRQEVQRPFTFGNDPLLRMVLYRVA
HSRWVLFINIHHIITDGWSMGVFFP

Specific function: Incorporates six amino acids (for tyrocidine A, Asn, Gln, Tyr, Val, Orn, and Leu) in their L-configuration into the peptide product [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=480, Percent_Identity=24.375, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI122937307, Length=528, Percent_Identity=24.2424242424242, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1786801, Length=631, Percent_Identity=29.7939778129952, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI145693145, Length=538, Percent_Identity=24.1635687732342, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786810, Length=540, Percent_Identity=24.0740740740741, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1788107, Length=230, Percent_Identity=25.6521739130435, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556356, Length=578, Percent_Identity=26.643598615917, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17550940, Length=590, Percent_Identity=22.8813559322034, Blast_Score=115, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319591, Length=671, Percent_Identity=23.0998509687034, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319699, Length=381, Percent_Identity=23.0971128608924, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648676, Length=507, Percent_Identity=30.1775147928994, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 83302; Mature: 83171

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLDRTLISAFTIHVKNTPDKVAIKHNGKQTTYRQLDELSTNIASYIWQRKPGHDKSTS
CCCHHHHHEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
ILICLERSELTIGVILACVKLGFTYVPFDPKNPDERLQYMLDDCRPALVITSKKDAKRQD
EEEEEECCCCHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
GYLEIYVEDIPLPTAEVSPDFPGVSNLEAVYIMYTSGSTGNPKGVVISQKGILRLVLDAK
CEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEEECC
PFQFSSDAIIAQSGNIAFDASTFEIWGALLNGATLVITPYETVIDSDALATFLCVETITD
CCCCCCCEEEEECCCEEEECHHHHHHHHHHCCCEEEEECHHHHHCCHHHHHHHHHHHHHH
AWFTVALFNLLVMENPGVFGSLNNVLIGGDALNPKLVHAVLCSDTPPGALWNGYGPTENT
HHHHHHHHHHHHCCCCCCEECCCCEEEECCCCCHHHEEEEEECCCCCCCCCCCCCCCCCC
VFTTLHRITLEDSTRSSIPIGIPIAGTHCYVLDADRQPVIPGEEGELYTSGAGLATGYLN
EEEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCEEECCCCEEECCCC
KPEKTAEAFFPNPYYKLEKCQNLQSASAWMYKTGDRVKMLADGTLDFLGRVDNQVKIRGF
CCCHHHHHCCCCCCHHHHHHCCHHHCCCEEEECCCEEEEEECCCHHHHHCCCCEEEEEEE
RLEPGEVEFQLCQLADVDLAVVTVVEHQGQKKLAAWCKSSRNANEILQLFRQQVPAYMVP
EECCCCCEEEEEEECCCCEEEEEEEHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
SYLQVVESFPVTANGKIDKRSLPAPQFNNHQATPPQTATEIWLSKTWQQLLSLPHPVGRE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
DNFSSLGGHSLLIVKLKQKIQTGLNKRVSLTELFNLSSLSDMAEYIDTLPESCGVITLNR
CCCCCCCCCEEEEEEEHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEC
VGYGEKIALTNEQLRLWLICNREPQLPHYSIPLVFQLSGELHPERLAQALDSLCQRHQSL
CCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RSRIFNTEGTPWQQVAEMSPQLIMDEVKDEDTLQEILRQEVQRPFTFGNDPLLRMVLYRV
HHHHCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AHSRWVLFINIHHIITDGWSMGVFFP
HCCCEEEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TMLDRTLISAFTIHVKNTPDKVAIKHNGKQTTYRQLDELSTNIASYIWQRKPGHDKSTS
CCHHHHHEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
ILICLERSELTIGVILACVKLGFTYVPFDPKNPDERLQYMLDDCRPALVITSKKDAKRQD
EEEEEECCCCHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
GYLEIYVEDIPLPTAEVSPDFPGVSNLEAVYIMYTSGSTGNPKGVVISQKGILRLVLDAK
CEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEEECC
PFQFSSDAIIAQSGNIAFDASTFEIWGALLNGATLVITPYETVIDSDALATFLCVETITD
CCCCCCCEEEEECCCEEEECHHHHHHHHHHCCCEEEEECHHHHHCCHHHHHHHHHHHHHH
AWFTVALFNLLVMENPGVFGSLNNVLIGGDALNPKLVHAVLCSDTPPGALWNGYGPTENT
HHHHHHHHHHHHCCCCCCEECCCCEEEECCCCCHHHEEEEEECCCCCCCCCCCCCCCCCC
VFTTLHRITLEDSTRSSIPIGIPIAGTHCYVLDADRQPVIPGEEGELYTSGAGLATGYLN
EEEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCCEEECCCCEEECCCC
KPEKTAEAFFPNPYYKLEKCQNLQSASAWMYKTGDRVKMLADGTLDFLGRVDNQVKIRGF
CCCHHHHHCCCCCCHHHHHHCCHHHCCCEEEECCCEEEEEECCCHHHHHCCCCEEEEEEE
RLEPGEVEFQLCQLADVDLAVVTVVEHQGQKKLAAWCKSSRNANEILQLFRQQVPAYMVP
EECCCCCEEEEEEECCCCEEEEEEEHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
SYLQVVESFPVTANGKIDKRSLPAPQFNNHQATPPQTATEIWLSKTWQQLLSLPHPVGRE
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
DNFSSLGGHSLLIVKLKQKIQTGLNKRVSLTELFNLSSLSDMAEYIDTLPESCGVITLNR
CCCCCCCCCEEEEEEEHHHHHHCCCCCEEHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEC
VGYGEKIALTNEQLRLWLICNREPQLPHYSIPLVFQLSGELHPERLAQALDSLCQRHQSL
CCCCCEEEEECCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
RSRIFNTEGTPWQQVAEMSPQLIMDEVKDEDTLQEILRQEVQRPFTFGNDPLLRMVLYRV
HHHHCCCCCCCHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AHSRWVLFINIHHIITDGWSMGVFFP
HCCCEEEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9352938; 10801488 [H]