Click here to switch to the map view.

The map label for this gene is entD [C]

Identifier: 238920282

GI number: 238920282

Start: 2287757

End: 2288533

Strand: Reverse

Name: entD [C]

Synonym: NT01EI_2392

Alternate gene names: 238920282

Gene position: 2288533-2287757 (Counterclockwise)

Preceding gene: 238920283

Following gene: 238920281

Centisome position: 60.03

GC content: 45.05

Gene sequence:

>777_bases
ATGATGTCTAATAGCCAATATCTACCCGGAGAAAGTGAATACACTCCGGGTAACATCGGGGGGCCTCCGGGTGTTGAAGT
AAACTCATCCGATTTTTTACATGATTACCGCCCATTTCCATTGCCCGACCTCAATGGCGGTATTATCGGCTGCCAGTTCT
TCAAACAATATTATGAAGACAGATTATTTTCGGCACTGGGTGTCAGTTTTCCTCCATCGCTAAATAAGGCCCTTCCTATA
CGCCGGGCGGAGTATCTTGCAGGGCGGGCACTGGCAAAAATAAATCTGGCACAGTTGCACATATTTGACCATCAGCTGCT
GCCGGACAGGAACGGGGTCCCACAATGGCCAACGTGTATTAGTGGCTCACTCAGTCATAATCGTGACATGGCGATATGTG
TAACTCTGCCATGTGAACAGGCCAGGATGGTCGGGATAGATATTGAAAAACTGATATCACCTGAGGATGCACAGCTATTA
TGGCGACATATTATCAGTCCTCAGGAGTATGCATTTTTGCTGACCCAACCACTGGACTTTCATCAAATGTTAACGCTGGT
TTTTTCTGCGAAAGAGAGCTTTTATAAGCTTGTTTTTCCTAAAACAAGAGCTGCGATGATTTTTCATAGCGTCAAAATTG
TCGCCTTTGACCTATCCTCTGGAGTGTTTTCGCTGACGATACCGGATGAGCTGATTGCTCTTTTTCCATCCCGGCGTTAT
ACCGAAGGAAGGTTTTTGTTAATGGATGATAATGTTATTACATTTATGCGTGCATGA

Upstream 100 bases:

>100_bases
CGGATGCTGATTACAGCATCGATTTTCCGAAACTGTTGCGGCTTTATACTGATTTCTGGCTGCAAAGTGGCTTCCTTGTG
GCACCGATGGATAAGTTGGC

Downstream 100 bases:

>100_bases
ATTAAATTTGTTAATGAAATGGTTTCCCCTATATCCTGTGCCACAGTGGGACGATAAATCCTCACTACTTGAGTGGCGTG
GTCATCATGAACGTTAAAAC

Product: phosphopantetheinyl transferase

Products: pyrophosphate; AMP; enterobactin; aryl carrier protein/isochorismatase; 3',5'-ADP; serine activating enzyme + [C]

Alternate protein names: Phosphopantetheinyl Transferase; Siderophore Biosynthesis Protein; 4-Phosphopantetheinyl Tranferase; 4\-Phosphopantetheinyl Tranferase; 4-Phosphopantetheinyl Transferase EntD; Non-Ribosomal Peptide Synthetase; 4-Phosphopantetheinyl Transferase Family Protein; Enterobactin Synthetase Component D; Enterochelin Synthetase Component D; Phosphopantetheinyltransferase Component; Vibriobactin Synthase Component D; 4-Phosphopantetheinyl Transferase Protein; 4-Phosphopantetheinyl Transferase Entd; Phosphopantetheinyl Transferase EntD

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MMSNSQYLPGESEYTPGNIGGPPGVEVNSSDFLHDYRPFPLPDLNGGIIGCQFFKQYYEDRLFSALGVSFPPSLNKALPI
RRAEYLAGRALAKINLAQLHIFDHQLLPDRNGVPQWPTCISGSLSHNRDMAICVTLPCEQARMVGIDIEKLISPEDAQLL
WRHIISPQEYAFLLTQPLDFHQMLTLVFSAKESFYKLVFPKTRAAMIFHSVKIVAFDLSSGVFSLTIPDELIALFPSRRY
TEGRFLLMDDNVITFMRA

Sequences:

>Translated_258_residues
MMSNSQYLPGESEYTPGNIGGPPGVEVNSSDFLHDYRPFPLPDLNGGIIGCQFFKQYYEDRLFSALGVSFPPSLNKALPI
RRAEYLAGRALAKINLAQLHIFDHQLLPDRNGVPQWPTCISGSLSHNRDMAICVTLPCEQARMVGIDIEKLISPEDAQLL
WRHIISPQEYAFLLTQPLDFHQMLTLVFSAKESFYKLVFPKTRAAMIFHSVKIVAFDLSSGVFSLTIPDELIALFPSRRY
TEGRFLLMDDNVITFMRA
>Mature_258_residues
MMSNSQYLPGESEYTPGNIGGPPGVEVNSSDFLHDYRPFPLPDLNGGIIGCQFFKQYYEDRLFSALGVSFPPSLNKALPI
RRAEYLAGRALAKINLAQLHIFDHQLLPDRNGVPQWPTCISGSLSHNRDMAICVTLPCEQARMVGIDIEKLISPEDAQLL
WRHIISPQEYAFLLTQPLDFHQMLTLVFSAKESFYKLVFPKTRAAMIFHSVKIVAFDLSSGVFSLTIPDELIALFPSRRY
TEGRFLLMDDNVITFMRA

Specific function: Catalyzes The Transfer Of The 4'-Phosphopantetheine Moiety From Coenzyme A To Apo-Domains Of Both Entb (An Arcp Domain) And Entf (A Pcp Domain). Plays An Essential Role In The Assembly Of The Enterobactin. [C]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786797, Length=136, Percent_Identity=34.5588235294118, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.8.- [C]

Molecular weight: Translated: 29132; Mature: 29132

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNSQYLPGESEYTPGNIGGPPGVEVNSSDFLHDYRPFPLPDLNGGIIGCQFFKQYYED
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHH
RLFSALGVSFPPSLNKALPIRRAEYLAGRALAKINLAQLHIFDHQLLPDRNGVPQWPTCI
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEEECHHCCCCCCCCCCCCCHH
SGSLSHNRDMAICVTLPCEQARMVGIDIEKLISPEDAQLLWRHIISPQEYAFLLTQPLDF
CCCCCCCCCEEEEEECCCCCCEEECCCHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCH
HQMLTLVFSAKESFYKLVFPKTRAAMIFHSVKIVAFDLSSGVFSLTIPDELIALFPSRRY
HHHHHHHHHHHHHHEEEECCCHHHHHHHEEEEEEEEECCCCEEEEECHHHHHHHCCCCCC
TEGRFLLMDDNVITFMRA
CCCEEEEEECCEEEEEEC
>Mature Secondary Structure
MMSNSQYLPGESEYTPGNIGGPPGVEVNSSDFLHDYRPFPLPDLNGGIIGCQFFKQYYED
CCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHH
RLFSALGVSFPPSLNKALPIRRAEYLAGRALAKINLAQLHIFDHQLLPDRNGVPQWPTCI
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEEECHHCCCCCCCCCCCCCHH
SGSLSHNRDMAICVTLPCEQARMVGIDIEKLISPEDAQLLWRHIISPQEYAFLLTQPLDF
CCCCCCCCCEEEEEECCCCCCEEECCCHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCH
HQMLTLVFSAKESFYKLVFPKTRAAMIFHSVKIVAFDLSSGVFSLTIPDELIALFPSRRY
HHHHHHHHHHHHHHEEEECCCHHHHHHHEEEEEEEEECCCCEEEEECHHHHHHHCCCCCC
TEGRFLLMDDNVITFMRA
CCCEEEEEECCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate; Coenzyme A; apo-EntB; apo-serine activating enzyme [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin Coenzyme A + apo-EntB = aryl carrier protein/isochorismatase + 3',5'-ADP Coenzyme A + apo-serine activating enzyme = 3',5'-ADP + serine activating enzyme [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA