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The map label for this gene is fumB [H]

Identifier: 238920276

GI number: 238920276

Start: 2284344

End: 2285990

Strand: Reverse

Name: fumB [H]

Synonym: NT01EI_2386

Alternate gene names: 238920276

Gene position: 2285990-2284344 (Counterclockwise)

Preceding gene: 238920277

Following gene: 238920272

Centisome position: 59.96

GC content: 59.81

Gene sequence:

>1647_bases
ATGTCAAATAAACCCTTCCATTATCAAGATCCGTTTCCGCTGCAAGGCGATGACACCGACTATTATCTGCTGAGTAAGGA
GCATGTTTCCGTGGTCAGCTTTGAAGGCCAGGAGATGCTGAAGGTCGAGCCGCAGGCGCTGACGCTGTTGGCCCAGCAGG
CGTTCCACGATGCCTCGTTTATGCTGCGCCCGGCTCATCAGCGCCAGGTCGCCGCGATCCTTGACGATCCGCAGGCCAGC
GAAAACGATAAATATGTCGCGCTGCAGTTCCTGCGTAACTCCGAAATCGCCGCCAAGGGGGTGCTGCCGACCTGTCAGGA
CACCGGGACGGCGATTATCGTGGGTAAAAAAGGCCAGCGCGTTTGGACCGGTGGCGGCGATGCCGAGGCGCTGTCGCGCG
GGGTTTATAACACCTATATCGAAGATAACCTGCGCTATTCCCAGAACGCCGCGCTGGATATGTACAATGAGGTCAATACC
GGGACCAACCTGCCGGCGCAGATCGATCTGTACGCCACTGAGGGCGAGGAGTACAAATTCCTGTGCGTCGCCAAAGGCGG
CGGCTCAGCCAATAAAACCTATCTGTATCAGGAGACCAAGGCGCTGCTCACGCCGGAGAAGCTGAAGGCTTTCCTGATCG
AGAAGATGCGTACCCTGGGCACCGCGGCCTGCCCGCCATACCACGTGGCCTTCGTCATCGGTGGCACCTCGGCGGAGTCT
ACCCTGAAGACGGTGAAACTGGCATCGACCAAGTACTATGATGCGTTGCCGACCGAGGGCAATGAATACGGCCAGGCGTT
CCGTGACGTGGCGCTGGAGCAGGAGCTGCTGCTGGCAGCCCAGGCGCTGGGGCTGGGCGCGCAGTTCGGCGGTAAATACT
TTGCCCACGATGTGCGGGTGATCCGCCTGCCGCGTCACGGTGCCTCCTGTCCGGTCGGCATGGGGGTTTCCTGCTCGGCG
GATCGCAATATTAAGGCCAAGATTAACCGCGAGGGGATCTGGATCGAGAAGTTGGAGCATAACCCGGGTAAATATATTCC
TGAGCATCTGCGCCAGGCCGGCGAGGGGGAGGCCATCAAGGTGGATCTCAACCGTCCGATGAAGGAGATTCTGGCGCAGC
TCTCCCAGTATCCGGTCTCCACCCGTCTGTCGCTTTCCGGCACCATCATCGTCGGGCGTGATATCGCCCACGCCAAGCTG
AAGGCGCTGCTGGACGAAGGCAAGCCGCTGCCGCAGTATGTGAAGGATCACCCGATCTACTATGCAGGCCCGGCCAAGAC
GCCGGCGGGCCATGCCTCTGGCTCACTGGGGCCAACCACGGCAGGCCGTATGGACTCCTATGTTGACCTGCTGCAGGCCA
ACGGCGGCAGCATGGTTATGCTGGCCAAAGGAAACCGCAGCCAGCAGGTCACCGACGCCTGCCATAAACACGGCGGCTTC
TATCTGGGCAGTATCGGCGGCCCAGCGGCGGTACTGGCCCAGCAGAGCATTAAGAGCCTGACCTGCGTGGCCTATCCTGA
GCTGGGAATGGAGGCTATCTGGAAGATTGAGGTAGAGGACTTCCCGGCCTTCATCCTGGTGGATGACAAGGGGAATGACT
TCTTTCAGCAGATTGTGACCAGCCGCTGCAGTGGCTGCATGAAATAA

Upstream 100 bases:

>100_bases
AAAGTTAACAGGTCGCGCGGTTTTTCTTATCGGGCATCACGGCGATGGCGCTGCGTGGCTCGGTAAATTTATACAAGCTG
ACGTTGTAAGCGAGAGAAAA

Downstream 100 bases:

>100_bases
GCGGCTAATCCCGGCAGCGATAGTGGCCGGTTATCCCATTGATCCCCACGCCATGGCGTGGGGATTTTTGTATGGGCCTA
GCGCAGCTGATCGCGGGCCG

Product: fumarate hydratase class I, anaerobic

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFHYQDPFPLQGDDTDYYLLSKEHVSVVSFEGQEMLKVEPQALTLLAQQAFHDASFMLRPAHQRQVAAILDDPQAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDAEALSRGVYNTYIEDNLRYSQNAALDMYNEVNT
GTNLPAQIDLYATEGEEYKFLCVAKGGGSANKTYLYQETKALLTPEKLKAFLIEKMRTLGTAACPPYHVAFVIGGTSAES
TLKTVKLASTKYYDALPTEGNEYGQAFRDVALEQELLLAAQALGLGAQFGGKYFAHDVRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGKYIPEHLRQAGEGEAIKVDLNRPMKEILAQLSQYPVSTRLSLSGTIIVGRDIAHAKL
KALLDEGKPLPQYVKDHPIYYAGPAKTPAGHASGSLGPTTAGRMDSYVDLLQANGGSMVMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLTCVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVTSRCSGCMK

Sequences:

>Translated_548_residues
MSNKPFHYQDPFPLQGDDTDYYLLSKEHVSVVSFEGQEMLKVEPQALTLLAQQAFHDASFMLRPAHQRQVAAILDDPQAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDAEALSRGVYNTYIEDNLRYSQNAALDMYNEVNT
GTNLPAQIDLYATEGEEYKFLCVAKGGGSANKTYLYQETKALLTPEKLKAFLIEKMRTLGTAACPPYHVAFVIGGTSAES
TLKTVKLASTKYYDALPTEGNEYGQAFRDVALEQELLLAAQALGLGAQFGGKYFAHDVRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGKYIPEHLRQAGEGEAIKVDLNRPMKEILAQLSQYPVSTRLSLSGTIIVGRDIAHAKL
KALLDEGKPLPQYVKDHPIYYAGPAKTPAGHASGSLGPTTAGRMDSYVDLLQANGGSMVMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKSLTCVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVTSRCSGCMK
>Mature_547_residues
SNKPFHYQDPFPLQGDDTDYYLLSKEHVSVVSFEGQEMLKVEPQALTLLAQQAFHDASFMLRPAHQRQVAAILDDPQASE
NDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDAEALSRGVYNTYIEDNLRYSQNAALDMYNEVNTG
TNLPAQIDLYATEGEEYKFLCVAKGGGSANKTYLYQETKALLTPEKLKAFLIEKMRTLGTAACPPYHVAFVIGGTSAEST
LKTVKLASTKYYDALPTEGNEYGQAFRDVALEQELLLAAQALGLGAQFGGKYFAHDVRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINREGIWIEKLEHNPGKYIPEHLRQAGEGEAIKVDLNRPMKEILAQLSQYPVSTRLSLSGTIIVGRDIAHAKLK
ALLDEGKPLPQYVKDHPIYYAGPAKTPAGHASGSLGPTTAGRMDSYVDLLQANGGSMVMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQQSIKSLTCVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVTSRCSGCMK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=87.956204379562, Blast_Score=1017, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=87.043795620438, Blast_Score=1007, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=28.7292817679558, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59709; Mature: 59577

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFHYQDPFPLQGDDTDYYLLSKEHVSVVSFEGQEMLKVEPQALTLLAQQAFHDASF
CCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCEEEECHHHHHHHHHHHHCCCHH
MLRPAHQRQVAAILDDPQASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHEEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDAEALSRGVYNTYIEDNLRYSQNAALDMYNEVNTGTNLPAQIDLYATEGEEYKF
EEECCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPEKLKAFLIEKMRTLGTAACPPYHVAFVIGGTSAES
EEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TLKTVKLASTKYYDALPTEGNEYGQAFRDVALEQELLLAAQALGLGAQFGGKYFAHDVRV
HHHEEEEECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGKYIPEHLRQAGEGEAIK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLSGTIIVGRDIAHAKLKALLDEGKPLPQYVKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEECEEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCEE
YAGPAKTPAGHASGSLGPTTAGRMDSYVDLLQANGGSMVMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLTCVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVT
EEECCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SRCSGCMK
HHHHCCCC
>Mature Secondary Structure 
SNKPFHYQDPFPLQGDDTDYYLLSKEHVSVVSFEGQEMLKVEPQALTLLAQQAFHDASF
CCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCEEEECHHHHHHHHHHHHCCCHH
MLRPAHQRQVAAILDDPQASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHEEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDAEALSRGVYNTYIEDNLRYSQNAALDMYNEVNTGTNLPAQIDLYATEGEEYKF
EEECCCCHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEEECCCCEEE
LCVAKGGGSANKTYLYQETKALLTPEKLKAFLIEKMRTLGTAACPPYHVAFVIGGTSAES
EEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHH
TLKTVKLASTKYYDALPTEGNEYGQAFRDVALEQELLLAAQALGLGAQFGGKYFAHDVRV
HHHEEEEECCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGKYIPEHLRQAGEGEAIK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCHHHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLSGTIIVGRDIAHAKLKALLDEGKPLPQYVKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEECEEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCEE
YAGPAKTPAGHASGSLGPTTAGRMDSYVDLLQANGGSMVMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKSLTCVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVT
EEECCCCHHHHHHHHHHHHEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
SRCSGCMK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]