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The map label for this gene is holB [H]

Identifier: 238920254

GI number: 238920254

Start: 2265407

End: 2266393

Strand: Reverse

Name: holB [H]

Synonym: NT01EI_2363

Alternate gene names: 238920254

Gene position: 2266393-2265407 (Counterclockwise)

Preceding gene: 238920255

Following gene: 238920253

Centisome position: 59.45

GC content: 66.77

Gene sequence:

>987_bases
GTGAACTGGTATCCCTGGCTCAACGCACCGTATCGCCAGCTGCTGGCACAGCATCAGGAGGGGCGTGGCCACCATGCGCT
GCTGCTGCATGCCATCGACGGCATGGGTGAGGAGTCGCTGGTATACGCTCTGGCCCGCTGGTTGCTTTGTCAGCGCCCGC
AGGGAGATAAGAGCTGTGGCGAATGCCATGCCTGCCGCCTGATGATGGCGGGCACCCACCCTGACTGGCACGTGCTGCAG
CCGGAGAAAGGGAAGTCGACGCTGGGTATTGAACCGGTGCGTCAGCTCATTGAGCAGCTGCAGAGCCATGCCCAGCAGGG
GGGAGCCAAGCTGGTGTGGATCGCCCGCAGCGAGCAGCTCAGCGAAGCGGCGGCCAATGCCCTGCTGAAGACGCTGGAGG
AGCCGCCCGCACGGACCTATTTCCTGCTGGGAACCCGCGAACCGGCGCAGTTGTTGCCGACGCTGCGCAGCCGCTGTTTC
TACCTCTATCTCGCTCCGCCGGCCGAGGGCGTCAGCCTGCGCTGGTTGACGCAGCAGATGCCGGGGGATGCGCAGGCCCA
GCGCAGTGCCCTGCGCCTGAGTGCAGGCGCACCGGCAGCAGCGCTGGCGCTACTGCAGCCGCCGCGCTGGACCCAGCGCG
AGGCGCTGTGTCAGGCGCTGTATCAGGCGTTGGAGAGCCGCCAGTGGCTGACGCTGCTGGGCGTGTTGAACCATGACGAC
GGTAGCGAACGGCTGGGCTGGCTGTGCGCCCTGCTGCTGGATGCCCTGAAGCTGTGCCACGGCGCCGCGGCCAGCGTCAC
TAACTGTGACCACCTCCCGTTAATCCAGCGGCTGGCGACGTGTCTCGGCGAGGCCGATCTGCGGCGCCTGCTGCAGGCAT
GGATCGCTTGTCGCCACCAGTTGCTGGCGACGCCGGGGGTAAACCGTGAATTGATGCTCATGCGCCAACTTTGCGTATGG
GACGCACCCGCACCGGCCCACCCGTGA

Upstream 100 bases:

>100_bases
AGACCATCGTAACTCTGGATGCCGGGCAGCCGCTGGAGCAGGTCAGTGCGGCGATCCGTCAGGCGCTACGGGCGTGGCTG
GCCTCTCAGGAGGCGCAGGT

Downstream 100 bases:

>100_bases
GCCGGTCATAGACAGACTGTAGGTTTCTTATGTATCTAGTGGATTCGCACTGCCATCTTGATGGCTTGAACTATGAGACG
CTGCACGACGGCGTCGACAG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNWYPWLNAPYRQLLAQHQEGRGHHALLLHAIDGMGEESLVYALARWLLCQRPQGDKSCGECHACRLMMAGTHPDWHVLQ
PEKGKSTLGIEPVRQLIEQLQSHAQQGGAKLVWIARSEQLSEAAANALLKTLEEPPARTYFLLGTREPAQLLPTLRSRCF
YLYLAPPAEGVSLRWLTQQMPGDAQAQRSALRLSAGAPAAALALLQPPRWTQREALCQALYQALESRQWLTLLGVLNHDD
GSERLGWLCALLLDALKLCHGAAASVTNCDHLPLIQRLATCLGEADLRRLLQAWIACRHQLLATPGVNRELMLMRQLCVW
DAPAPAHP

Sequences:

>Translated_328_residues
MNWYPWLNAPYRQLLAQHQEGRGHHALLLHAIDGMGEESLVYALARWLLCQRPQGDKSCGECHACRLMMAGTHPDWHVLQ
PEKGKSTLGIEPVRQLIEQLQSHAQQGGAKLVWIARSEQLSEAAANALLKTLEEPPARTYFLLGTREPAQLLPTLRSRCF
YLYLAPPAEGVSLRWLTQQMPGDAQAQRSALRLSAGAPAAALALLQPPRWTQREALCQALYQALESRQWLTLLGVLNHDD
GSERLGWLCALLLDALKLCHGAAASVTNCDHLPLIQRLATCLGEADLRRLLQAWIACRHQLLATPGVNRELMLMRQLCVW
DAPAPAHP
>Mature_328_residues
MNWYPWLNAPYRQLLAQHQEGRGHHALLLHAIDGMGEESLVYALARWLLCQRPQGDKSCGECHACRLMMAGTHPDWHVLQ
PEKGKSTLGIEPVRQLIEQLQSHAQQGGAKLVWIARSEQLSEAAANALLKTLEEPPARTYFLLGTREPAQLLPTLRSRCF
YLYLAPPAEGVSLRWLTQQMPGDAQAQRSALRLSAGAPAAALALLQPPRWTQREALCQALYQALESRQWLTLLGVLNHDD
GSERLGWLCALLLDALKLCHGAAASVTNCDHLPLIQRLATCLGEADLRRLLQAWIACRHQLLATPGVNRELMLMRQLCVW
DAPAPAHP

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=313, Percent_Identity=53.9936102236422, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1786676, Length=144, Percent_Identity=36.1111111111111, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36522; Mature: 36522

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWYPWLNAPYRQLLAQHQEGRGHHALLLHAIDGMGEESLVYALARWLLCQRPQGDKSCG
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
ECHACRLMMAGTHPDWHVLQPEKGKSTLGIEPVRQLIEQLQSHAQQGGAKLVWIARSEQL
HHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHH
SEAAANALLKTLEEPPARTYFLLGTREPAQLLPTLRSRCFYLYLAPPAEGVSLRWLTQQM
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCEEEEEECCCCCCCEEEHHHHHC
PGDAQAQRSALRLSAGAPAAALALLQPPRWTQREALCQALYQALESRQWLTLLGVLNHDD
CCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
GSERLGWLCALLLDALKLCHGAAASVTNCDHLPLIQRLATCLGEADLRRLLQAWIACRHQ
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LLATPGVNRELMLMRQLCVWDAPAPAHP
HHCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNWYPWLNAPYRQLLAQHQEGRGHHALLLHAIDGMGEESLVYALARWLLCQRPQGDKSCG
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
ECHACRLMMAGTHPDWHVLQPEKGKSTLGIEPVRQLIEQLQSHAQQGGAKLVWIARSEQL
HHHHHHHHHCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHH
SEAAANALLKTLEEPPARTYFLLGTREPAQLLPTLRSRCFYLYLAPPAEGVSLRWLTQQM
HHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCEEEEEECCCCCCCEEEHHHHHC
PGDAQAQRSALRLSAGAPAAALALLQPPRWTQREALCQALYQALESRQWLTLLGVLNHDD
CCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
GSERLGWLCALLLDALKLCHGAAASVTNCDHLPLIQRLATCLGEADLRRLLQAWIACRHQ
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHH
LLATPGVNRELMLMRQLCVWDAPAPAHP
HHCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430; 10491164 [H]