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The map label for this gene is rluE [H]

Identifier: 238920207

GI number: 238920207

Start: 2214314

End: 2214841

Strand: Direct

Name: rluE [H]

Synonym: NT01EI_2316

Alternate gene names: 238920207

Gene position: 2214314-2214841 (Clockwise)

Preceding gene: 238920205

Following gene: 238920208

Centisome position: 58.08

GC content: 62.88

Gene sequence:

>528_bases
TTGAATAAGCCCTTCGACGTCCTGCCGCAGTTTACCGATGAGCAGGGGCGCGCCACCCTGAAAGACTATATCCCGCTGCA
GGGGGTTTACGCCGCGGGGCGGCTGGATCGCGACAGCGAGGGGCTGCTGGTGCTGACCAACGATGGTCGACTGCAGGCGC
AGCTGACCCAACCCGGCAAGCGCACCGGCAAAGTCTACTATGTGCAGGTCGAGGGTGAACCTAGCGAGGCGGATCTGCAG
CGCTTTTGCCAGGGGCTGACGCTGAAGGATGGCCCAACCCTGCCCGCCCTGGCCGAGCGCGTCGCGCAGCCGGATTGGCT
ATGGCCGCGCAACCCACCGATCCGTGAGCGTAAAAACATCCCGACCAGCTGGCTTAAAATCACTCTGTATGAAGGGCGCA
ACCGCCAGGTCCGCCGCATGACGGCCCATATCGGTTACCCTACACTGCGCCTGATCCGCTATAGTATGGACTCTCTAACC
CTGCAGGATCTGGCACCGGGAGAATGGCGCCGGATCGACTGGCCCTGA

Upstream 100 bases:

>100_bases
GTCAAAAACAGCCCCCATTTCCCGCGCTCGGCGCCCGGCGTCATCCTCCCGCCGTCCCAGTATAGCCAAACGCCCCGCGG
CGCCGCGTCAGGTGGTGATT

Downstream 100 bases:

>100_bases
GCTCCTCTTGCCTCAGCGCCGCAACGGAGACGTCACCGTGCTCAAACCCCATGTGACCGTAGCCTGCGTAGTTCAGGCTG
AAGGACATTTTTTAATCGTG

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MNKPFDVLPQFTDEQGRATLKDYIPLQGVYAAGRLDRDSEGLLVLTNDGRLQAQLTQPGKRTGKVYYVQVEGEPSEADLQ
RFCQGLTLKDGPTLPALAERVAQPDWLWPRNPPIRERKNIPTSWLKITLYEGRNRQVRRMTAHIGYPTLRLIRYSMDSLT
LQDLAPGEWRRIDWP

Sequences:

>Translated_175_residues
MNKPFDVLPQFTDEQGRATLKDYIPLQGVYAAGRLDRDSEGLLVLTNDGRLQAQLTQPGKRTGKVYYVQVEGEPSEADLQ
RFCQGLTLKDGPTLPALAERVAQPDWLWPRNPPIRERKNIPTSWLKITLYEGRNRQVRRMTAHIGYPTLRLIRYSMDSLT
LQDLAPGEWRRIDWP
>Mature_175_residues
MNKPFDVLPQFTDEQGRATLKDYIPLQGVYAAGRLDRDSEGLLVLTNDGRLQAQLTQPGKRTGKVYYVQVEGEPSEADLQ
RFCQGLTLKDGPTLPALAERVAQPDWLWPRNPPIRERKNIPTSWLKITLYEGRNRQVRRMTAHIGYPTLRLIRYSMDSLT
LQDLAPGEWRRIDWP

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=172, Percent_Identity=76.1627906976744, Blast_Score=276, Evalue=7e-76,
Organism=Escherichia coli, GI1787524, Length=175, Percent_Identity=33.7142857142857, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1788510, Length=145, Percent_Identity=33.1034482758621, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790453, Length=171, Percent_Identity=29.8245614035088, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20159; Mature: 20159

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKPFDVLPQFTDEQGRATLKDYIPLQGVYAAGRLDRDSEGLLVLTNDGRLQAQLTQPGK
CCCCCCCCCCCCCCCCCEEHHHCCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCC
RTGKVYYVQVEGEPSEADLQRFCQGLTLKDGPTLPALAERVAQPDWLWPRNPPIRERKNI
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHCCC
PTSWLKITLYEGRNRQVRRMTAHIGYPTLRLIRYSMDSLTLQDLAPGEWRRIDWP
CCEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHEEHHHCCCCCCEECCCC
>Mature Secondary Structure
MNKPFDVLPQFTDEQGRATLKDYIPLQGVYAAGRLDRDSEGLLVLTNDGRLQAQLTQPGK
CCCCCCCCCCCCCCCCCEEHHHCCCCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCC
RTGKVYYVQVEGEPSEADLQRFCQGLTLKDGPTLPALAERVAQPDWLWPRNPPIRERKNI
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHCCC
PTSWLKITLYEGRNRQVRRMTAHIGYPTLRLIRYSMDSLTLQDLAPGEWRRIDWP
CCEEEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHEEHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]