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The map label for this gene is cbiJ [H]

Identifier: 238920079

GI number: 238920079

Start: 2109894

End: 2110679

Strand: Reverse

Name: cbiJ [H]

Synonym: NT01EI_2184

Alternate gene names: 238920079

Gene position: 2110679-2109894 (Counterclockwise)

Preceding gene: 238920080

Following gene: 238920078

Centisome position: 55.36

GC content: 68.19

Gene sequence:

>786_bases
GTGAACCATGGCGGGGTGCTGGTGATCGGCGGCACCAGCGACGCCCGGGCGCTGTGTCAGCAGCTGGATGCGGCCCGGGT
GGCCTACACCCTGTCGGTGGCGACGCCGACCGGTGAGGCCCTCGCCGGCGCGATCCGCGGGCGGGTGCGCTGCGGGCGCA
TGACGCAGGCGGAGATGGTCGCCTGGCTTGGCGCGCACCGGACCCGCTGGGTGATCGACGCATCGCATCCCTATGCCGAG
CTGGTCAGCCACAGTATCACGCAGGCCTGTCAGATGGCAGGGGTGCTGCTCAGCCGCTATCAGCGCCCGGAGCAGCTCGG
CGATCTGACCCATCCGCTGCTCCACCGCGTCGCCGGTATTGAGGCGGCCAGCGCGCTGGCGCGGCGCTTTGGCGAGCGGG
TATTGCTAACCACCGGCAGCAAGGATCTGGCGCGCTGGTGCGCGGCGCTGCCGGAGAAAACGCTGCTGGTGCGCGTGCTG
CCGCTGGCGGAGGTGATGGCGCAGTGCGCCCGGCTCGGCCTTGGCGTCGGGCAGATCTTCGCGCTGTGCGGCCCCTTCAG
CGCAGAGTTTAATGCGGCGTTTTATCGCCAATGCCGGGCTGACGTGGTGATAACCAAAGCCTCCGGTGCCGAGGGCGGCT
ATCGGGAAAAGGTTCAGCCCTGTCTGGATGCCGGTATTCCCTGCATCGTTATTACACGTCCGGCGCCGCTGGTGACGGGT
GCGGAGTTACTGGAGAGTCAGGCCGCCTTTCAACGCCGTTTAGCGCGCTGGCTGGCCATGGCTTAA

Upstream 100 bases:

>100_bases
TGGATTTTGCGCCGGTGGATATGACCAGTCTGGTGATCGTCGGCAATAAGGCCACCTACGTGCAGGACGGCCTGATGATC
ACGCCGCGGGGCTACGCGCT

Downstream 100 bases:

>100_bases
GGAGAATGAGATGAAAAAGGCGCTTCTGGTGGTCAGCTTTGGCACCAGCTACCGTGAGACCGCAGAGAAAAATATCGCAG
CCTGCGAGCGTGAGCTGGCG

Product: cobalt-precorrin-6x reductase

Products: Precorrin 6A; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNHGGVLVIGGTSDARALCQQLDAARVAYTLSVATPTGEALAGAIRGRVRCGRMTQAEMVAWLGAHRTRWVIDASHPYAE
LVSHSITQACQMAGVLLSRYQRPEQLGDLTHPLLHRVAGIEAASALARRFGERVLLTTGSKDLARWCAALPEKTLLVRVL
PLAEVMAQCARLGLGVGQIFALCGPFSAEFNAAFYRQCRADVVITKASGAEGGYREKVQPCLDAGIPCIVITRPAPLVTG
AELLESQAAFQRRLARWLAMA

Sequences:

>Translated_261_residues
MNHGGVLVIGGTSDARALCQQLDAARVAYTLSVATPTGEALAGAIRGRVRCGRMTQAEMVAWLGAHRTRWVIDASHPYAE
LVSHSITQACQMAGVLLSRYQRPEQLGDLTHPLLHRVAGIEAASALARRFGERVLLTTGSKDLARWCAALPEKTLLVRVL
PLAEVMAQCARLGLGVGQIFALCGPFSAEFNAAFYRQCRADVVITKASGAEGGYREKVQPCLDAGIPCIVITRPAPLVTG
AELLESQAAFQRRLARWLAMA
>Mature_261_residues
MNHGGVLVIGGTSDARALCQQLDAARVAYTLSVATPTGEALAGAIRGRVRCGRMTQAEMVAWLGAHRTRWVIDASHPYAE
LVSHSITQACQMAGVLLSRYQRPEQLGDLTHPLLHRVAGIEAASALARRFGERVLLTTGSKDLARWCAALPEKTLLVRVL
PLAEVMAQCARLGLGVGQIFALCGPFSAEFNAAFYRQCRADVVITKASGAEGGYREKVQPCLDAGIPCIVITRPAPLVTG
AELLESQAAFQRRLARWLAMA

Specific function: Catalyzes the reduction of the macrocycle of cobalt- precorrin-6X into cobalt-precorrin-6Y [H]

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003723 [H]

Pfam domain/function: PF02571 CbiJ [H]

EC number: 1.3.1.54

Molecular weight: Translated: 28001; Mature: 28001

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHGGVLVIGGTSDARALCQQLDAARVAYTLSVATPTGEALAGAIRGRVRCGRMTQAEMV
CCCCCEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHH
AWLGAHRTRWVIDASHPYAELVSHSITQACQMAGVLLSRYQRPEQLGDLTHPLLHRVAGI
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EAASALARRFGERVLLTTGSKDLARWCAALPEKTLLVRVLPLAEVMAQCARLGLGVGQIF
HHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALCGPFSAEFNAAFYRQCRADVVITKASGAEGGYREKVQPCLDAGIPCIVITRPAPLVTG
HHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHH
AELLESQAAFQRRLARWLAMA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNHGGVLVIGGTSDARALCQQLDAARVAYTLSVATPTGEALAGAIRGRVRCGRMTQAEMV
CCCCCEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHH
AWLGAHRTRWVIDASHPYAELVSHSITQACQMAGVLLSRYQRPEQLGDLTHPLLHRVAGI
HHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EAASALARRFGERVLLTTGSKDLARWCAALPEKTLLVRVLPLAEVMAQCARLGLGVGQIF
HHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ALCGPFSAEFNAAFYRQCRADVVITKASGAEGGYREKVQPCLDAGIPCIVITRPAPLVTG
HHHCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHH
AELLESQAAFQRRLARWLAMA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Precorrin 6B; NADP+

Specific reaction: precorrin-6B + NADP+ = precorrin-6A + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]