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The map label for this gene is cbiK [H]

Identifier: 238920078

GI number: 238920078

Start: 2109089

End: 2109883

Strand: Reverse

Name: cbiK [H]

Synonym: NT01EI_2183

Alternate gene names: 238920078

Gene position: 2109883-2109089 (Counterclockwise)

Preceding gene: 238920079

Following gene: 238920077

Centisome position: 55.34

GC content: 63.02

Gene sequence:

>795_bases
ATGAAAAAGGCGCTTCTGGTGGTCAGCTTTGGCACCAGCTACCGTGAGACCGCAGAGAAAAATATCGCAGCCTGCGAGCG
TGAGCTGGCGAACCATTGCCCCGATCGCGATCTGTTCCGGGCATTTACCTCCGGGATGATCATCCGCAAGCTCAGAGCGC
GCGACGGAATTGCGATCGACACGCCGCTGCAGGCGCTGCAGCGGCTTGCGGAGCAGGGATATCAGGATGTCGCGATTCAG
TCGCTGCACATTATTAACGGCGATGAGTATGAGAAGGTGGTGCGCGAGGTACAGAGTATGCGTCCGCTGTTTTTCCGCCT
GACGCTGGGGGCTCCGCTGCTGAGCAGCCATGATGACTACGTATCGCTGATGGGGGCGCTGCGGCAGCAGATGCCGCGCC
TCGGGGCGCAGGAGAAAGTGGTGTTTATGGGGCACGGCGCGACGCATCACGCCTTTGCGGCCTATGCCTGCCTCGATCAC
ATGATGCGGGCGCAGGGTCTTCCGGCCTGCGTGGGCGCGGTGGAGAGCTATCCGCAGGTCGAGATCCTGATCGAGAGCCT
CCGTCGGGAGGGGGTCCGCGCGGTGCATCTGATGCCGCTGATGCTGGTGGCCGGTGATCATGCGATCAATGACATGGCCT
CAGATGAGGCGGACTCCTGGAAAACGCAGTTCAACCGCGCCGGGATCCCGGCGACGCCGTGGCTGAACGGTCTGGGTGAG
AATCCCGCCGTACGGGCGATCTTCGTGGCGCACCTGCAGCAGGCGCTGAGCGCTGCCATGGAGGCGGCGGCATGA

Upstream 100 bases:

>100_bases
CGTCCGGCGCCGCTGGTGACGGGTGCGGAGTTACTGGAGAGTCAGGCCGCCTTTCAACGCCGTTTAGCGCGCTGGCTGGC
CATGGCTTAAGGAGAATGAG

Downstream 100 bases:

>100_bases
GCGGCAAACTGTACGCCCTGAGTACGGGGCCGGGCGCCGCCGATCTGATCACCGTCCGCGCCGCGCGGATCCTCGGCTCG
CTGGATATCCTCTACGCCCC

Product: sirohydrochlorin cobaltochelatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKKALLVVSFGTSYRETAEKNIAACERELANHCPDRDLFRAFTSGMIIRKLRARDGIAIDTPLQALQRLAEQGYQDVAIQ
SLHIINGDEYEKVVREVQSMRPLFFRLTLGAPLLSSHDDYVSLMGALRQQMPRLGAQEKVVFMGHGATHHAFAAYACLDH
MMRAQGLPACVGAVESYPQVEILIESLRREGVRAVHLMPLMLVAGDHAINDMASDEADSWKTQFNRAGIPATPWLNGLGE
NPAVRAIFVAHLQQALSAAMEAAA

Sequences:

>Translated_264_residues
MKKALLVVSFGTSYRETAEKNIAACERELANHCPDRDLFRAFTSGMIIRKLRARDGIAIDTPLQALQRLAEQGYQDVAIQ
SLHIINGDEYEKVVREVQSMRPLFFRLTLGAPLLSSHDDYVSLMGALRQQMPRLGAQEKVVFMGHGATHHAFAAYACLDH
MMRAQGLPACVGAVESYPQVEILIESLRREGVRAVHLMPLMLVAGDHAINDMASDEADSWKTQFNRAGIPATPWLNGLGE
NPAVRAIFVAHLQQALSAAMEAAA
>Mature_264_residues
MKKALLVVSFGTSYRETAEKNIAACERELANHCPDRDLFRAFTSGMIIRKLRARDGIAIDTPLQALQRLAEQGYQDVAIQ
SLHIINGDEYEKVVREVQSMRPLFFRLTLGAPLLSSHDDYVSLMGALRQQMPRLGAQEKVVFMGHGATHHAFAAYACLDH
MMRAQGLPACVGAVESYPQVEILIESLRREGVRAVHLMPLMLVAGDHAINDMASDEADSWKTQFNRAGIPATPWLNGLGE
NPAVRAIFVAHLQQALSAAMEAAA

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 29077; Mature: 29077

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALLVVSFGTSYRETAEKNIAACERELANHCPDRDLFRAFTSGMIIRKLRARDGIAID
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEC
TPLQALQRLAEQGYQDVAIQSLHIINGDEYEKVVREVQSMRPLFFRLTLGAPLLSSHDDY
CHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCHHH
VSLMGALRQQMPRLGAQEKVVFMGHGATHHAFAAYACLDHMMRAQGLPACVGAVESYPQV
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHH
EILIESLRREGVRAVHLMPLMLVAGDHAINDMASDEADSWKTQFNRAGIPATPWLNGLGE
HHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHCCCC
NPAVRAIFVAHLQQALSAAMEAAA
CHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKALLVVSFGTSYRETAEKNIAACERELANHCPDRDLFRAFTSGMIIRKLRARDGIAID
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEC
TPLQALQRLAEQGYQDVAIQSLHIINGDEYEKVVREVQSMRPLFFRLTLGAPLLSSHDDY
CHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHCCCHHH
VSLMGALRQQMPRLGAQEKVVFMGHGATHHAFAAYACLDHMMRAQGLPACVGAVESYPQV
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHH
EILIESLRREGVRAVHLMPLMLVAGDHAINDMASDEADSWKTQFNRAGIPATPWLNGLGE
HHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHCCCC
NPAVRAIFVAHLQQALSAAMEAAA
CHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]