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The map label for this gene is purB [H]

Identifier: 238920039

GI number: 238920039

Start: 2073711

End: 2075075

Strand: Reverse

Name: purB [H]

Synonym: NT01EI_2143

Alternate gene names: 238920039

Gene position: 2075075-2073711 (Counterclockwise)

Preceding gene: 238920040

Following gene: 238920038

Centisome position: 54.43

GC content: 51.65

Gene sequence:

>1365_bases
ATGTCTACCGGTGTTTTTGACTCAGCGTTGCTAAAAAACCTATGGTCTACTGAAGAAATGCGTGAAATCTTCTCTGACAA
TATTCGGATACAGATGTGGCTGGATTACGAGGCCGCGCTGGCTATTGAGCAGGCTGAGCTAGGCATTATTCCCAGCGAAG
CCGCTGATGAGATAGTGGCATGTGCCAACGTAGAATGTTTGGATATGGATTACATTCTGGAACAGGTCAGATTGACCAAA
CACCCGCTGGTTCCGACTATCCGCGGCCTAGAGCACGCCTGTGCTAAGGGATTCGGCGAGTATGTGCATTTCGGTCCGAC
GACTCAAGATGTGATGGATACCGGGATGATGCTGCAATTTAAAATGGCCCATAACGTCTTTTTACGTGAGCTGAAATCCA
TTGGCCGTGCATTGCTAACCCTATCGGAAACTCACCGTAATACGCCGATGGCGGGTCGTACTCTGGCCCTGCAGGCCATT
CCGATTACCTTTGGCCACAAGAGTGCTATCTGGCTTGCTGAGATTACCCGCCATTATGAGCGTCTGGTGCAGCTGGAGCC
GCGCTTCTTTGTTGGAAGTGTGGTTGGCGCAGTGGGGAGCAAAGCCTCATTTGGAGAGCGGGCTGATGAGTTGGAGCGGC
GCGTTCTGTCACGTTTGGGGTTGAACGTGCCGGAGATCTCTTGGCAGCCGGCCCGTGACCGCTTCTGTGAATATGGCATG
GTTCTCGGGATGATCGGTGCGACGCTGGGGAAAATTGCCAATGAAATTCTCTTGCTGGCCCATAATGAGCTGGATGAAGT
GGCCGAGCCCTTTGCTAAGGGGCAGGTCGGCTCCTCTACAATGCCCCATAAACGTAACCCCGCGACGACCGAGAATGCGG
CCTGTGTTAGTAATACACTGAAAGGCAACGTTTCCCTACTTCTCGACCTAATGAAACACCAGCATGAGCGTGATGGTGCC
ATCTGGAAAATGGAGTGGAAGGTTCTGCCTGAGATTTGCCTGATGTTATCCATCATCTTGGATAACATGAAATTTACCTT
GTCAGGTCTGGTGGTAAATAAAAATAAGATGCGCAGTAATCTCGATCTTCTCGGTGGCTTTATGCTGGCAGAGCGGGTCA
TGTTTATTCTCTCCGAGAAGGCTGGAAAGCAGACTGCCCATGAGTTGATCTATGAGGCCGCAATGCAGGGGCTGGAAGGG
GGAACTACCTTTGCCCACGCCCTGACCGAAAATCCCCGAATCATGGCGATCCTCAGTCAGTCTGAGCTGAATGCAGCCTT
AGATCCCACCACCTATATTGGTAACGCACCAGAGCAGGTGGATCAGGTTATCGCGCAAGTCAACGCGAAGGGATGGCTGA
ATTAG

Upstream 100 bases:

>100_bases
GTTGGCAGGTTGTTTTTTTCAGAAAATATTTCCATTGGTGTAAGCTAATGTAACGATATTTCTCTGGTTGGTGGATTCGA
CTATCAGGAGATTAAATACG

Downstream 100 bases:

>100_bases
TTACGGGGGATGGCGGGGGAGGCCTACCGCAGTACTCCAGGGGCGTTATCCCTGCATTGAGAGTTCTCCGATGACTATTC
TGGAAAATATGTCCTGATTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSTGVFDSALLKNLWSTEEMREIFSDNIRIQMWLDYEAALAIEQAELGIIPSEAADEIVACANVECLDMDYILEQVRLTK
HPLVPTIRGLEHACAKGFGEYVHFGPTTQDVMDTGMMLQFKMAHNVFLRELKSIGRALLTLSETHRNTPMAGRTLALQAI
PITFGHKSAIWLAEITRHYERLVQLEPRFFVGSVVGAVGSKASFGERADELERRVLSRLGLNVPEISWQPARDRFCEYGM
VLGMIGATLGKIANEILLLAHNELDEVAEPFAKGQVGSSTMPHKRNPATTENAACVSNTLKGNVSLLLDLMKHQHERDGA
IWKMEWKVLPEICLMLSIILDNMKFTLSGLVVNKNKMRSNLDLLGGFMLAERVMFILSEKAGKQTAHELIYEAAMQGLEG
GTTFAHALTENPRIMAILSQSELNAALDPTTYIGNAPEQVDQVIAQVNAKGWLN

Sequences:

>Translated_454_residues
MSTGVFDSALLKNLWSTEEMREIFSDNIRIQMWLDYEAALAIEQAELGIIPSEAADEIVACANVECLDMDYILEQVRLTK
HPLVPTIRGLEHACAKGFGEYVHFGPTTQDVMDTGMMLQFKMAHNVFLRELKSIGRALLTLSETHRNTPMAGRTLALQAI
PITFGHKSAIWLAEITRHYERLVQLEPRFFVGSVVGAVGSKASFGERADELERRVLSRLGLNVPEISWQPARDRFCEYGM
VLGMIGATLGKIANEILLLAHNELDEVAEPFAKGQVGSSTMPHKRNPATTENAACVSNTLKGNVSLLLDLMKHQHERDGA
IWKMEWKVLPEICLMLSIILDNMKFTLSGLVVNKNKMRSNLDLLGGFMLAERVMFILSEKAGKQTAHELIYEAAMQGLEG
GTTFAHALTENPRIMAILSQSELNAALDPTTYIGNAPEQVDQVIAQVNAKGWLN
>Mature_453_residues
STGVFDSALLKNLWSTEEMREIFSDNIRIQMWLDYEAALAIEQAELGIIPSEAADEIVACANVECLDMDYILEQVRLTKH
PLVPTIRGLEHACAKGFGEYVHFGPTTQDVMDTGMMLQFKMAHNVFLRELKSIGRALLTLSETHRNTPMAGRTLALQAIP
ITFGHKSAIWLAEITRHYERLVQLEPRFFVGSVVGAVGSKASFGERADELERRVLSRLGLNVPEISWQPARDRFCEYGMV
LGMIGATLGKIANEILLLAHNELDEVAEPFAKGQVGSSTMPHKRNPATTENAACVSNTLKGNVSLLLDLMKHQHERDGAI
WKMEWKVLPEICLMLSIILDNMKFTLSGLVVNKNKMRSNLDLLGGFMLAERVMFILSEKAGKQTAHELIYEAAMQGLEGG
TTFAHALTENPRIMAILSQSELNAALDPTTYIGNAPEQVDQVIAQVNAKGWLN

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=461, Percent_Identity=26.0303687635575, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI183227688, Length=399, Percent_Identity=25.062656641604, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1787376, Length=455, Percent_Identity=23.956043956044, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17508577, Length=412, Percent_Identity=23.0582524271845, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=365, Percent_Identity=22.4657534246575, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323391, Length=462, Percent_Identity=26.4069264069264, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24647570, Length=459, Percent_Identity=24.8366013071895, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50391; Mature: 50260

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGVFDSALLKNLWSTEEMREIFSDNIRIQMWLDYEAALAIEQAELGIIPSEAADEIVA
CCCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEEECHHHHHEEEHHHCCCCCCHHHHHHHH
CANVECLDMDYILEQVRLTKHPLVPTIRGLEHACAKGFGEYVHFGPTTQDVMDTGMMLQF
HCCCCEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHCCCHHEEH
KMAHNVFLRELKSIGRALLTLSETHRNTPMAGRTLALQAIPITFGHKSAIWLAEITRHYE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
RLVQLEPRFFVGSVVGAVGSKASFGERADELERRVLSRLGLNVPEISWQPARDRFCEYGM
HHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VLGMIGATLGKIANEILLLAHNELDEVAEPFAKGQVGSSTMPHKRNPATTENAACVSNTL
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KGNVSLLLDLMKHQHERDGAIWKMEWKVLPEICLMLSIILDNMKFTLSGLVVNKNKMRSN
CCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEHEEEEEHHHHHHH
LDLLGGFMLAERVMFILSEKAGKQTAHELIYEAAMQGLEGGTTFAHALTENPRIMAILSQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEC
SELNAALDPTTYIGNAPEQVDQVIAQVNAKGWLN
HHCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
STGVFDSALLKNLWSTEEMREIFSDNIRIQMWLDYEAALAIEQAELGIIPSEAADEIVA
CCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEEECHHHHHEEEHHHCCCCCCHHHHHHHH
CANVECLDMDYILEQVRLTKHPLVPTIRGLEHACAKGFGEYVHFGPTTQDVMDTGMMLQF
HCCCCEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHCCCHHEEH
KMAHNVFLRELKSIGRALLTLSETHRNTPMAGRTLALQAIPITFGHKSAIWLAEITRHYE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHH
RLVQLEPRFFVGSVVGAVGSKASFGERADELERRVLSRLGLNVPEISWQPARDRFCEYGM
HHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
VLGMIGATLGKIANEILLLAHNELDEVAEPFAKGQVGSSTMPHKRNPATTENAACVSNTL
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KGNVSLLLDLMKHQHERDGAIWKMEWKVLPEICLMLSIILDNMKFTLSGLVVNKNKMRSN
CCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEHEEEEEHHHHHHH
LDLLGGFMLAERVMFILSEKAGKQTAHELIYEAAMQGLEGGTTFAHALTENPRIMAILSQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEEEEC
SELNAALDPTTYIGNAPEQVDQVIAQVNAKGWLN
HHCCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]