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The map label for this gene is iolD [H]

Identifier: 238920032

GI number: 238920032

Start: 2064582

End: 2066507

Strand: Reverse

Name: iolD [H]

Synonym: NT01EI_2136

Alternate gene names: 238920032

Gene position: 2066507-2064582 (Counterclockwise)

Preceding gene: 238920033

Following gene: 238920031

Centisome position: 54.21

GC content: 63.4

Gene sequence:

>1926_bases
ATGAAAACCGAACGGATGACCATGGCGCAGGCGCTGGTCAAATTCCTTAATCAACAGTATGTCTGTGTCGACGGGCAGGA
GACGCCCTTTGTGGAAGGGGTAGCGACAATTTTCGGCCACGGCAATGTGCTCGGTATCGGGCAGGCGCTGGAGCAGGATG
CGGGTCACCTGAAGGTTATGCAGGGCTGCAACGAGCAGGGGATGGCGCATATGGCGACCGGGTTCGCGAAGCAGCACCGC
CGTCTGCGCATTTTTGCCGTTACCTCGTCGGTTGGGCCGGGGGCGGCCAACATGGTAACGGCGGCGGCTACCGCCACGGC
GAACCGTATTCCGCTGCTGCTGCTGCCGGGAGATATCTACGCCAGCCGCCAGCCGGACCCGGTGCTGCAGCAAATCGAGC
AGTTCCACGATCTGAGCATCAGCACCAACGATTGCTTTCGTCCGGTCTCCCGCTACTGGGATCGAATCAACCGCCCGGAG
CAGCTGATGAGCGCGATGCTGAACGCCATGCGTACGCTCACCGACCCGGCGGCCACCGGCGCGGTGACGATTTGCCTACC
GCAGGACGTGCAGGGGGAGGCCTGGGATTACCCGCACAGCTTCTTCGCCCGCCGCGTGCATTACATTGAACGCCGCCCGC
CGGACGTGCAGCGTCTGGCGATGGCACAGGCACTGATTGCCCGCAAGCGCAGGCCGCTGGTGGTGTGCGGTGGCGGGGTT
CGCTACTCGGGCGCTCACGATACATTTCGCGAGTTTGTCGAGACGCTGCAGCTGCCGTTTGCCGAAACGCAGGCCGGAAA
AGGGGCGCTGGCAAGCGATCATCCGCTGAACCTCGGTGGAATTGGCGTCACCGGCGGGCTGGCGGCGAATCAGCTGGCGC
CGCAGTCGGATTTGGTGATCGGTGTCGGTACACGCCTGAGCGATTTCACCACCGGTTCGAAGGCGCTGTTTTCCCACCCT
GATGTCGACTTCCTGCTGCTCAACGTTGCGGAGTTCGACGCACTCAAGCTCGACGCCACGGCGCTGGTGGCCGATGCCCG
CGTCGGGCTTTCGGCGTTGACGGCGGCGCTTGCCGGTTACCGAAGCCAGTGGGGCGACGCGGTTTGCCGGGCGATGGCGG
CCTGGCGCAACGAGTGCTGCCGTCTGTGGACGCGGACATGGTCGCCCGGTGAGGTGCCGGAAATCGCCGGACATCTTGAT
GCGCTGCTGGGCGAGTATGGCGAGACGCTCAACACCCGTCTGACCCAGACGCGGGTGCTGGGGCTTATCAACCAGCATAT
TGAAGAGGACGCCATCGTGGTCGGTGCGGCGGGCTCTCTGCCGGGGGATCTGCAGCGTCTGTGGCAGGTGAAGACGCCCG
ACAGCTACCATCTGGAGTACGGCTACTCCTGCATGGGCTATGAGATTGCCGCCGCCATTGGCGCTAAGCTCGCCATGCCG
CAGCAGCCGGTCTATGCCATGGTGGGCGACGGCTCGTACATGATGCTGCACTCTGAGCTGCAAACGGCAGTGCAGGAGGG
AATTAAGATTAGCGTGCTGCTGTTTGATAACGCCGGGTTTGGCTGCATCAACAACCTGCAGATGGGGCATGGCATCGGCA
GCTTTGGCACAGAGAGCCGTCAGCGCAATCCGCAAACCGGGCGGCTGGACGGTCCGCTGGTGAAGGTGGATTTCGCACAG
AACGCCGAAAGCTACGGCTGCCGTGCATGGCGGGTACACGATGAGTCAAGTCTGCTGGCGGCGCTGGCGGCGTCCCGGCA
GCATCCCGGGCCGACGCTTATCGATATCAAGGTGTTGCCAAAGACCATGACGCACGACTATGCCGCCTGGTGGCGAACCG
GTGATGCGCAGGTTGCAACATCGCCCGCGGTACGTGACGCGGCGGAGCAGACACAAAACCGGGTGAAAAGCGCGCGCCAG
TATTGA

Upstream 100 bases:

>100_bases
TCTATACCCGGATGAAGACCGTTACCGCCCGCTGGCCTGAGATGCAACAGGATACCGGCGCCGCGTTCTCCATGCCGACG
CTGGGCTGACAGGAGGGGTC

Downstream 100 bases:

>100_bases
GTTTTCCAGCGCGACGAAATCTTTATTCCATTCTCTCTTTTCCGCGTGCCCCCACTCCCCAAAAGGGCGTGGGGGCACGC
GCAGTTGCGGCCGTATCCTC

Product: malonic semialdehyde oxidative decarboxylase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MKTERMTMAQALVKFLNQQYVCVDGQETPFVEGVATIFGHGNVLGIGQALEQDAGHLKVMQGCNEQGMAHMATGFAKQHR
RLRIFAVTSSVGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQFHDLSISTNDCFRPVSRYWDRINRPE
QLMSAMLNAMRTLTDPAATGAVTICLPQDVQGEAWDYPHSFFARRVHYIERRPPDVQRLAMAQALIARKRRPLVVCGGGV
RYSGAHDTFREFVETLQLPFAETQAGKGALASDHPLNLGGIGVTGGLAANQLAPQSDLVIGVGTRLSDFTTGSKALFSHP
DVDFLLLNVAEFDALKLDATALVADARVGLSALTAALAGYRSQWGDAVCRAMAAWRNECCRLWTRTWSPGEVPEIAGHLD
ALLGEYGETLNTRLTQTRVLGLINQHIEEDAIVVGAAGSLPGDLQRLWQVKTPDSYHLEYGYSCMGYEIAAAIGAKLAMP
QQPVYAMVGDGSYMMLHSELQTAVQEGIKISVLLFDNAGFGCINNLQMGHGIGSFGTESRQRNPQTGRLDGPLVKVDFAQ
NAESYGCRAWRVHDESSLLAALAASRQHPGPTLIDIKVLPKTMTHDYAAWWRTGDAQVATSPAVRDAAEQTQNRVKSARQ
Y

Sequences:

>Translated_641_residues
MKTERMTMAQALVKFLNQQYVCVDGQETPFVEGVATIFGHGNVLGIGQALEQDAGHLKVMQGCNEQGMAHMATGFAKQHR
RLRIFAVTSSVGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQFHDLSISTNDCFRPVSRYWDRINRPE
QLMSAMLNAMRTLTDPAATGAVTICLPQDVQGEAWDYPHSFFARRVHYIERRPPDVQRLAMAQALIARKRRPLVVCGGGV
RYSGAHDTFREFVETLQLPFAETQAGKGALASDHPLNLGGIGVTGGLAANQLAPQSDLVIGVGTRLSDFTTGSKALFSHP
DVDFLLLNVAEFDALKLDATALVADARVGLSALTAALAGYRSQWGDAVCRAMAAWRNECCRLWTRTWSPGEVPEIAGHLD
ALLGEYGETLNTRLTQTRVLGLINQHIEEDAIVVGAAGSLPGDLQRLWQVKTPDSYHLEYGYSCMGYEIAAAIGAKLAMP
QQPVYAMVGDGSYMMLHSELQTAVQEGIKISVLLFDNAGFGCINNLQMGHGIGSFGTESRQRNPQTGRLDGPLVKVDFAQ
NAESYGCRAWRVHDESSLLAALAASRQHPGPTLIDIKVLPKTMTHDYAAWWRTGDAQVATSPAVRDAAEQTQNRVKSARQ
Y
>Mature_641_residues
MKTERMTMAQALVKFLNQQYVCVDGQETPFVEGVATIFGHGNVLGIGQALEQDAGHLKVMQGCNEQGMAHMATGFAKQHR
RLRIFAVTSSVGPGAANMVTAAATATANRIPLLLLPGDIYASRQPDPVLQQIEQFHDLSISTNDCFRPVSRYWDRINRPE
QLMSAMLNAMRTLTDPAATGAVTICLPQDVQGEAWDYPHSFFARRVHYIERRPPDVQRLAMAQALIARKRRPLVVCGGGV
RYSGAHDTFREFVETLQLPFAETQAGKGALASDHPLNLGGIGVTGGLAANQLAPQSDLVIGVGTRLSDFTTGSKALFSHP
DVDFLLLNVAEFDALKLDATALVADARVGLSALTAALAGYRSQWGDAVCRAMAAWRNECCRLWTRTWSPGEVPEIAGHLD
ALLGEYGETLNTRLTQTRVLGLINQHIEEDAIVVGAAGSLPGDLQRLWQVKTPDSYHLEYGYSCMGYEIAAAIGAKLAMP
QQPVYAMVGDGSYMMLHSELQTAVQEGIKISVLLFDNAGFGCINNLQMGHGIGSFGTESRQRNPQTGRLDGPLVKVDFAQ
NAESYGCRAWRVHDESSLLAALAASRQHPGPTLIDIKVLPKTMTHDYAAWWRTGDAQVATSPAVRDAAEQTQNRVKSARQ
Y

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=609, Percent_Identity=25.2873563218391, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081685, Length=611, Percent_Identity=28.3142389525368, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786717, Length=583, Percent_Identity=23.4991423670669, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1788716, Length=520, Percent_Identity=21.5384615384615, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=23.9388794567063, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922626, Length=602, Percent_Identity=22.7574750830565, Blast_Score=84, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69699; Mature: 69699

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTERMTMAQALVKFLNQQYVCVDGQETPFVEGVATIFGHGNVLGIGQALEQDAGHLKVM
CCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHHCCCHHHHH
QGCNEQGMAHMATGFAKQHRRLRIFAVTSSVGPGAANMVTAAATATANRIPLLLLPGDIY
HCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
ASRQPDPVLQQIEQFHDLSISTNDCFRPVSRYWDRINRPEQLMSAMLNAMRTLTDPAATG
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDYPHSFFARRVHYIERRPPDVQRLAMAQALIARKRRPLVVCGGGV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCE
RYSGAHDTFREFVETLQLPFAETQAGKGALASDHPLNLGGIGVTGGLAANQLAPQSDLVI
EECCCHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEE
GVGTRLSDFTTGSKALFSHPDVDFLLLNVAEFDALKLDATALVADARVGLSALTAALAGY
EECCCCCCCCCCCCHHCCCCCCCEEEEEHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHH
RSQWGDAVCRAMAAWRNECCRLWTRTWSPGEVPEIAGHLDALLGEYGETLNTRLTQTRVL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLINQHIEEDAIVVGAAGSLPGDLQRLWQVKTPDSYHLEYGYSCMGYEIAAAIGAKLAMP
HHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
QQPVYAMVGDGSYMMLHSELQTAVQEGIKISVLLFDNAGFGCINNLQMGHGIGSFGTESR
CCCCEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHH
QRNPQTGRLDGPLVKVDFAQNAESYGCRAWRVHDESSLLAALAASRQHPGPTLIDIKVLP
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEEEECC
KTMTHDYAAWWRTGDAQVATSPAVRDAAEQTQNRVKSARQY
CHHCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTERMTMAQALVKFLNQQYVCVDGQETPFVEGVATIFGHGNVLGIGQALEQDAGHLKVM
CCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCCEEECCHHHHHCCCHHHHH
QGCNEQGMAHMATGFAKQHRRLRIFAVTSSVGPGAANMVTAAATATANRIPLLLLPGDIY
HCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
ASRQPDPVLQQIEQFHDLSISTNDCFRPVSRYWDRINRPEQLMSAMLNAMRTLTDPAATG
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AVTICLPQDVQGEAWDYPHSFFARRVHYIERRPPDVQRLAMAQALIARKRRPLVVCGGGV
EEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEECCCE
RYSGAHDTFREFVETLQLPFAETQAGKGALASDHPLNLGGIGVTGGLAANQLAPQSDLVI
EECCCHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCEEE
GVGTRLSDFTTGSKALFSHPDVDFLLLNVAEFDALKLDATALVADARVGLSALTAALAGY
EECCCCCCCCCCCCHHCCCCCCCEEEEEHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHH
RSQWGDAVCRAMAAWRNECCRLWTRTWSPGEVPEIAGHLDALLGEYGETLNTRLTQTRVL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLINQHIEEDAIVVGAAGSLPGDLQRLWQVKTPDSYHLEYGYSCMGYEIAAAIGAKLAMP
HHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCC
QQPVYAMVGDGSYMMLHSELQTAVQEGIKISVLLFDNAGFGCINNLQMGHGIGSFGTESR
CCCCEEEECCCCEEEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHH
QRNPQTGRLDGPLVKVDFAQNAESYGCRAWRVHDESSLLAALAASRQHPGPTLIDIKVLP
CCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHCCCCCCEEEEEEECC
KTMTHDYAAWWRTGDAQVATSPAVRDAAEQTQNRVKSARQY
CHHCCHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA