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The map label for this gene is bioD2 [H]

Identifier: 238919966

GI number: 238919966

Start: 1990364

End: 1991029

Strand: Reverse

Name: bioD2 [H]

Synonym: NT01EI_2070

Alternate gene names: 238919966

Gene position: 1991029-1990364 (Counterclockwise)

Preceding gene: 238919967

Following gene: 238919964

Centisome position: 52.23

GC content: 55.26

Gene sequence:

>666_bases
ATGCTAAAACGCGTATTCGTTACGGGAACCGACACCGAGGTGGGTAAAACGGTGGCTTCCCGTGCGTTATTACAAGCTAT
TCAGGCAGCTGGGCACAGTGCTGCGGGATATAAACCTATCGCCGTAGGCAGTGATGAGACCCCTCAGGGGGTACGCAACC
GGGATGCCGTAACTCTGCTCAAGGCATCCTCCATGGTGCTAAGCTATGACGAAGTCAACCCCGTAACCCTGCGTGAAGAG
ATAGCCAATGCCTATACCGGCGAAGCGCTGGACTATGGCGCCATGAGCCGTGGGTTACAGAATATGAGTGTCAAGGCGCA
ACATGTTGTGGTTCAGGGAACCGGCGGCTGGCGGGTCTTGATGCAGGATTTACGTCCGCTCTCAGAGTGGGTTGTGCAGG
AGCAGCTTCCGGTAATCCTGGTGGTAGGGATTAAGCTGGGCTGTATCAGTCATGCCATCTTGACCGCTCAGGCCATTATT
AATGATGGCCTGCCGTTTGTCGGCTGGATTGCGAACCGGATTAACCCAGGTCTGTCGCACTATGCTGAAACCATTGATGC
CCTGCGGCAGAAGCTGCCGGCACCGCAGTTGGGTGAGATCCCCTATCTGTACCGTCCAGAGGAAAAAGCGTTGGCGCGCT
ACATCGATTTATCTGCGCTGGACTGA

Upstream 100 bases:

>100_bases
ACTTTCTGCTTTATTGGCGATCGCGGGTACACAGCATACTTCCGGTCAGATCGTTCTGCTTTCCAATTATTAATCGGAGT
CTCTCCGGAGTAATTATTCA

Downstream 100 bases:

>100_bases
AGCCGCGCATGGGTAGATGTTGTTACTGAAACGGGCGTGCATAGTGCACGCCCGTTTGCTTTTCATTAACGGCTACGGCT
ATGGATGGGCCGCCTCCACC

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase 2; DTBS 2; Dethiobiotin synthase 2 [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLKRVFVTGTDTEVGKTVASRALLQAIQAAGHSAAGYKPIAVGSDETPQGVRNRDAVTLLKASSMVLSYDEVNPVTLREE
IANAYTGEALDYGAMSRGLQNMSVKAQHVVVQGTGGWRVLMQDLRPLSEWVVQEQLPVILVVGIKLGCISHAILTAQAII
NDGLPFVGWIANRINPGLSHYAETIDALRQKLPAPQLGEIPYLYRPEEKALARYIDLSALD

Sequences:

>Translated_221_residues
MLKRVFVTGTDTEVGKTVASRALLQAIQAAGHSAAGYKPIAVGSDETPQGVRNRDAVTLLKASSMVLSYDEVNPVTLREE
IANAYTGEALDYGAMSRGLQNMSVKAQHVVVQGTGGWRVLMQDLRPLSEWVVQEQLPVILVVGIKLGCISHAILTAQAII
NDGLPFVGWIANRINPGLSHYAETIDALRQKLPAPQLGEIPYLYRPEEKALARYIDLSALD
>Mature_221_residues
MLKRVFVTGTDTEVGKTVASRALLQAIQAAGHSAAGYKPIAVGSDETPQGVRNRDAVTLLKASSMVLSYDEVNPVTLREE
IANAYTGEALDYGAMSRGLQNMSVKAQHVVVQGTGGWRVLMQDLRPLSEWVVQEQLPVILVVGIKLGCISHAILTAQAII
NDGLPFVGWIANRINPGLSHYAETIDALRQKLPAPQLGEIPYLYRPEEKALARYIDLSALD

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=220, Percent_Identity=62.2727272727273, Blast_Score=293, Evalue=4e-81,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=52, Blast_Score=224, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 23945; Mature: 23945

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRVFVTGTDTEVGKTVASRALLQAIQAAGHSAAGYKPIAVGSDETPQGVRNRDAVTLL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHEE
KASSMVLSYDEVNPVTLREEIANAYTGEALDYGAMSRGLQNMSVKAQHVVVQGTGGWRVL
ECCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHCCCEEEEEEEEECCCCHHHH
MQDLRPLSEWVVQEQLPVILVVGIKLGCISHAILTAQAIINDGLPFVGWIANRINPGLSH
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
YAETIDALRQKLPAPQLGEIPYLYRPEEKALARYIDLSALD
HHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKRVFVTGTDTEVGKTVASRALLQAIQAAGHSAAGYKPIAVGSDETPQGVRNRDAVTLL
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCHHHEE
KASSMVLSYDEVNPVTLREEIANAYTGEALDYGAMSRGLQNMSVKAQHVVVQGTGGWRVL
ECCCCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHCHHCCCEEEEEEEEECCCCHHHH
MQDLRPLSEWVVQEQLPVILVVGIKLGCISHAILTAQAIINDGLPFVGWIANRINPGLSH
HHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
YAETIDALRQKLPAPQLGEIPYLYRPEEKALARYIDLSALD
HHHHHHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]