Click here to switch to the map view.

The map label for this gene is ppsA [H]

Identifier: 238919812

GI number: 238919812

Start: 1845160

End: 1847535

Strand: Direct

Name: ppsA [H]

Synonym: NT01EI_1916

Alternate gene names: 238919812

Gene position: 1845160-1847535 (Clockwise)

Preceding gene: 238919798

Following gene: 238919814

Centisome position: 48.4

GC content: 60.44

Gene sequence:

>2376_bases
ATGTCTAGTAATGGCGCGCAATTACAGAATGTGGTGTGGTACAACCAGCTCGGGATGCACGATGTCGAGAGGGTAGGCGG
TAAAAATGCCTCCCTGGGAGAGATGATAACCAACCTGTCTGAACTGGGTGTGTCGGTGCCGAATGGCTTTGCCACTACGG
CGCAGGCGTTTAATGATTTTCTGGAACAGAGCGGCGTCAATCAGCGGATCTATACGCTGTTGGATCAGACGGACATCGAT
GATATAGACGCGCTGGCGCGGGCGGGGGCGCAGATCCGCCGCCTGGTGATCGAAACCCCGTTTCCTGCGGCGCTGGAGGC
GGAAATCCGCAGCGCCTATGCCACCTTGGCCGCGGACAACGTCAATGCCTCCTTCGCCGTGCGCTCCTCGGCGACGGCAG
AGGATATGCCTGATGCCTCGTTTGCCGGGCAGCAGGAGACCTTTCTCAACGTACAGGGCATCGATGCGGTGATGACGGCG
GTGAAGCATGTTTTCGCCTCGCTGTTTAACGATCGTGCCATTTCATACCGGGTGCACCAGGGCTATGACCACCGCGGCGT
GGCACTCTCTGCCGGGGTTCAGCTGATGGTACGCTCCGATCTGGCAGCCTCTGGAGTGATGTTTACCCTGGATACCGAAT
CGGGGTTTGATCAGGTGGTGTTTATCACCTCGGCCTATGGGCTGGGCGAAATGGTGGTGCAGGGAGCGGTAAACCCCGAT
GAGTTCTACGTGCATAAACCGACCCTGCAGGCGGGGCGGCCGGCCATCGTGCGCCGTACGCTGGGGTCGAAGAAGATCCG
TATGGTGTATGCCGACAGCCAGGAGCACGGTAGGCAGGTGCGGATTGAGGATGTCCCTGCGGTGCAGAGCGGGCGTTTTA
GCCTGAGCGACGGGGAGATCCAGCAGCTGGCCCGGCAGGCGCTGCTGATCGAACAGCACTATGGCCGTCCGATGGACATT
GAATGGGCCAAGGACGGGCACAGTGGGCGGCTGTACATCGTTCAGGCCCGTCCGGAGACCGTACGGGCCAACGAGCAGGT
GATGGAGCGCTATCAGTTGAGCCAGCGCGGCGAGGTGCTGGTGGAGGGACGCGCCATCGGCCATCGTATCGGCGCCGGGC
CGGTGAAGGTGATCACCAGTATCAGTGAGATGCACCTGATCCAGGCGGGCGATGTGCTGGTGACGGACATGACCGATCCG
GACTGGGAGCCGATCATGAAGCGGGCCTCGGCGATTGTCACCAACCGCGGCGGGCGCACCTGTCATGCGGCGATCATCGC
GCGCGAACTGGGGATCCCGGCGGTCGTCGGTTGCGGCGATGCCACCGAGCGCCTGCGACCGGGGGCGGCGGTCACAGTTT
CCTGCGCCGAAGGGGATACCGGCTACGTTTACCACGGGCAGATCGACTTCAGCGTACAGAGTTCGCAGGTAGAGCAGATG
CCGGCGCTTCCCCTGAAGATCATGATGAACGTCGGTAACCCGGACCGCGCCTTTGACTTCGCCCGTCTGCCCAACGACGG
CGTGGGGCTGGCGCGTCTGGAGTTTATCATTAACCGGATGATCGGCGTACATCCGCGTGCGGCACTGGAGTTTGAGCGAC
AGGATGCGACGGTGCAGGCGGAGATCCGTCAGATGATGCAGGGATTTGACGATCCGGTGGAGTTCTACATCAGCCGACTG
ACTGAGGGTATTGCGACGCTGGCGGCGGCATTCTGGCCCAAACGGGTGATTGTCCGTCTGTCAGACTTCAAGTCCAATGA
ATATGCCAACCTGATTGGCGGTGAGCACTATGAGCCCCATGAGGAAAACCCGATGTTGGGGTTCCGCGGCGCCGGGCGCT
ATGTGGCGGAGAGCTTCGCCCCCTGTTTTGCGCTGGAGTGCGCTGCCATTAAACGGGTGCGCAATACGATGGGGCTGACC
AACGTCGAGGTGATGATCCCCTTTGTGCGTACGGTGGAGCAGGCGCAGGCGGTGGTTTCTCAGTTGGCGAAAGAGGGGCT
GCGCCGAGGTGAAAATGGCCTGAAGGTGATCATGATGTGCGAGATCCCCTCCAATGCGCTGTTGGCCGACGCATTTCTGG
CCCATTTTGACGGTTTCTCCATCGGCTCTAACGATATGACTCAGCTCACCCTGGGCCTGGATCGCGACTCTGGAGTGGTC
TCTGCGCTGTTTGACGAGCGCAATGCGGCGGTGAAAGCACTGCTGTCGATGGCTATCCGCAGCGCCAAGGCGCAGGGGAA
ATATGTGGGGATCTGCGGTCAGGGTCCTTCGGATCATGAGGATTTTGCCCGCTGGCTCATGGAGCAGGGAATTGACAGTC
TGTCACTGAATCCTGATACGGTGGCGCAGACCTGGATTAATCTGGCAGAAAAATAA

Upstream 100 bases:

>100_bases
ATCTATCTTGGCCTGTGTCGACATTATGTTAGTCTGATTACGCCGCGTTGGCGGTATCTTGTCTCCGAAACCCATGCTTT
GAAATAAAAGGATTGCTTCG

Downstream 100 bases:

>100_bases
GCGTTATTTTGATAATAGTCTAAAACGGTCGCTGTAATGCGGCCGTTTTTTCTGCCATTTTTATTTTTTCTCGACGCGTC
AAAAATAAACGGCCGGTTGC

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 791; Mature: 790

Protein sequence:

>791_residues
MSSNGAQLQNVVWYNQLGMHDVERVGGKNASLGEMITNLSELGVSVPNGFATTAQAFNDFLEQSGVNQRIYTLLDQTDID
DIDALARAGAQIRRLVIETPFPAALEAEIRSAYATLAADNVNASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAVMTA
VKHVFASLFNDRAISYRVHQGYDHRGVALSAGVQLMVRSDLAASGVMFTLDTESGFDQVVFITSAYGLGEMVVQGAVNPD
EFYVHKPTLQAGRPAIVRRTLGSKKIRMVYADSQEHGRQVRIEDVPAVQSGRFSLSDGEIQQLARQALLIEQHYGRPMDI
EWAKDGHSGRLYIVQARPETVRANEQVMERYQLSQRGEVLVEGRAIGHRIGAGPVKVITSISEMHLIQAGDVLVTDMTDP
DWEPIMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLRPGAAVTVSCAEGDTGYVYHGQIDFSVQSSQVEQM
PALPLKIMMNVGNPDRAFDFARLPNDGVGLARLEFIINRMIGVHPRAALEFERQDATVQAEIRQMMQGFDDPVEFYISRL
TEGIATLAAAFWPKRVIVRLSDFKSNEYANLIGGEHYEPHEENPMLGFRGAGRYVAESFAPCFALECAAIKRVRNTMGLT
NVEVMIPFVRTVEQAQAVVSQLAKEGLRRGENGLKVIMMCEIPSNALLADAFLAHFDGFSIGSNDMTQLTLGLDRDSGVV
SALFDERNAAVKALLSMAIRSAKAQGKYVGICGQGPSDHEDFARWLMEQGIDSLSLNPDTVAQTWINLAEK

Sequences:

>Translated_791_residues
MSSNGAQLQNVVWYNQLGMHDVERVGGKNASLGEMITNLSELGVSVPNGFATTAQAFNDFLEQSGVNQRIYTLLDQTDID
DIDALARAGAQIRRLVIETPFPAALEAEIRSAYATLAADNVNASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAVMTA
VKHVFASLFNDRAISYRVHQGYDHRGVALSAGVQLMVRSDLAASGVMFTLDTESGFDQVVFITSAYGLGEMVVQGAVNPD
EFYVHKPTLQAGRPAIVRRTLGSKKIRMVYADSQEHGRQVRIEDVPAVQSGRFSLSDGEIQQLARQALLIEQHYGRPMDI
EWAKDGHSGRLYIVQARPETVRANEQVMERYQLSQRGEVLVEGRAIGHRIGAGPVKVITSISEMHLIQAGDVLVTDMTDP
DWEPIMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLRPGAAVTVSCAEGDTGYVYHGQIDFSVQSSQVEQM
PALPLKIMMNVGNPDRAFDFARLPNDGVGLARLEFIINRMIGVHPRAALEFERQDATVQAEIRQMMQGFDDPVEFYISRL
TEGIATLAAAFWPKRVIVRLSDFKSNEYANLIGGEHYEPHEENPMLGFRGAGRYVAESFAPCFALECAAIKRVRNTMGLT
NVEVMIPFVRTVEQAQAVVSQLAKEGLRRGENGLKVIMMCEIPSNALLADAFLAHFDGFSIGSNDMTQLTLGLDRDSGVV
SALFDERNAAVKALLSMAIRSAKAQGKYVGICGQGPSDHEDFARWLMEQGIDSLSLNPDTVAQTWINLAEK
>Mature_790_residues
SSNGAQLQNVVWYNQLGMHDVERVGGKNASLGEMITNLSELGVSVPNGFATTAQAFNDFLEQSGVNQRIYTLLDQTDIDD
IDALARAGAQIRRLVIETPFPAALEAEIRSAYATLAADNVNASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAVMTAV
KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQLMVRSDLAASGVMFTLDTESGFDQVVFITSAYGLGEMVVQGAVNPDE
FYVHKPTLQAGRPAIVRRTLGSKKIRMVYADSQEHGRQVRIEDVPAVQSGRFSLSDGEIQQLARQALLIEQHYGRPMDIE
WAKDGHSGRLYIVQARPETVRANEQVMERYQLSQRGEVLVEGRAIGHRIGAGPVKVITSISEMHLIQAGDVLVTDMTDPD
WEPIMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLRPGAAVTVSCAEGDTGYVYHGQIDFSVQSSQVEQMP
ALPLKIMMNVGNPDRAFDFARLPNDGVGLARLEFIINRMIGVHPRAALEFERQDATVQAEIRQMMQGFDDPVEFYISRLT
EGIATLAAAFWPKRVIVRLSDFKSNEYANLIGGEHYEPHEENPMLGFRGAGRYVAESFAPCFALECAAIKRVRNTMGLTN
VEVMIPFVRTVEQAQAVVSQLAKEGLRRGENGLKVIMMCEIPSNALLADAFLAHFDGFSIGSNDMTQLTLGLDRDSGVVS
ALFDERNAAVKALLSMAIRSAKAQGKYVGICGQGPSDHEDFARWLMEQGIDSLSLNPDTVAQTWINLAEK

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=790, Percent_Identity=81.0126582278481, Blast_Score=1342, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=442, Percent_Identity=25.5656108597285, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=34.5549738219895, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994992, Length=372, Percent_Identity=26.3440860215054, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789193, Length=406, Percent_Identity=24.384236453202, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=345, Percent_Identity=26.9565217391304, Blast_Score=89, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86420; Mature: 86289

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNGAQLQNVVWYNQLGMHDVERVGGKNASLGEMITNLSELGVSVPNGFATTAQAFNDF
CCCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LEQSGVNQRIYTLLDQTDIDDIDALARAGAQIRRLVIETPFPAALEAEIRSAYATLAADN
HHHCCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHEECC
VNASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAVMTAVKHVFASLFNDRAISYRVHQ
CCCEEEEECCCCCCCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GYDHRGVALSAGVQLMVRSDLAASGVMFTLDTESGFDQVVFITSAYGLGEMVVQGAVNPD
CCCCCCEEECCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCC
EFYVHKPTLQAGRPAIVRRTLGSKKIRMVYADSQEHGRQVRIEDVPAVQSGRFSLSDGEI
CEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCHHCCCEEEEECCCCCCCCCEECCCHHH
QQLARQALLIEQHYGRPMDIEWAKDGHSGRLYIVQARPETVRANEQVMERYQLSQRGEVL
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCCHHCCCHHHHHHHHHHCCCCEE
VEGRAIGHRIGAGPVKVITSISEMHLIQAGDVLVTDMTDPDWEPIMKRASAIVTNRGGRT
EECCHHCCCCCCCHHHHHHHHHHHHEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
CHAAIIARELGIPAVVGCGDATERLRPGAAVTVSCAEGDTGYVYHGQIDFSVQSSQVEQM
HHHHHHHHHCCCCEEEECCCHHHHCCCCCEEEEEECCCCCCEEEEEEEEEEECHHHHHHC
PALPLKIMMNVGNPDRAFDFARLPNDGVGLARLEFIINRMIGVHPRAALEFERQDATVQA
CCCCEEEEEECCCCHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHEEECCCCCHHHH
EIRQMMQGFDDPVEFYISRLTEGIATLAAAFWPKRVIVRLSDFKSNEYANLIGGEHYEPH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHCCCCCCCCC
EENPMLGFRGAGRYVAESFAPCFALECAAIKRVRNTMGLTNVEVMIPFVRTVEQAQAVVS
CCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QLAKEGLRRGENGLKVIMMCEIPSNALLADAFLAHFDGFSIGSNDMTQLTLGLDRDSGVV
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
SALFDERNAAVKALLSMAIRSAKAQGKYVGICGQGPSDHEDFARWLMEQGIDSLSLNPDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHH
VAQTWINLAEK
HHHHHHHHHCC
>Mature Secondary Structure 
SSNGAQLQNVVWYNQLGMHDVERVGGKNASLGEMITNLSELGVSVPNGFATTAQAFNDF
CCCCCHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LEQSGVNQRIYTLLDQTDIDDIDALARAGAQIRRLVIETPFPAALEAEIRSAYATLAADN
HHHCCCCHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHEECC
VNASFAVRSSATAEDMPDASFAGQQETFLNVQGIDAVMTAVKHVFASLFNDRAISYRVHQ
CCCEEEEECCCCCCCCCCCCCCCCCHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GYDHRGVALSAGVQLMVRSDLAASGVMFTLDTESGFDQVVFITSAYGLGEMVVQGAVNPD
CCCCCCEEECCCEEEEEECCCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCC
EFYVHKPTLQAGRPAIVRRTLGSKKIRMVYADSQEHGRQVRIEDVPAVQSGRFSLSDGEI
CEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCHHCCCEEEEECCCCCCCCCEECCCHHH
QQLARQALLIEQHYGRPMDIEWAKDGHSGRLYIVQARPETVRANEQVMERYQLSQRGEVL
HHHHHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCCHHCCCHHHHHHHHHHCCCCEE
VEGRAIGHRIGAGPVKVITSISEMHLIQAGDVLVTDMTDPDWEPIMKRASAIVTNRGGRT
EECCHHCCCCCCCHHHHHHHHHHHHEEECCCEEEECCCCCCHHHHHHHHHHHHCCCCCCC
CHAAIIARELGIPAVVGCGDATERLRPGAAVTVSCAEGDTGYVYHGQIDFSVQSSQVEQM
HHHHHHHHHCCCCEEEECCCHHHHCCCCCEEEEEECCCCCCEEEEEEEEEEECHHHHHHC
PALPLKIMMNVGNPDRAFDFARLPNDGVGLARLEFIINRMIGVHPRAALEFERQDATVQA
CCCCEEEEEECCCCHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHEEECCCCCHHHH
EIRQMMQGFDDPVEFYISRLTEGIATLAAAFWPKRVIVRLSDFKSNEYANLIGGEHYEPH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHEEEEECCCCCCCHHHCCCCCCCCC
EENPMLGFRGAGRYVAESFAPCFALECAAIKRVRNTMGLTNVEVMIPFVRTVEQAQAVVS
CCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QLAKEGLRRGENGLKVIMMCEIPSNALLADAFLAHFDGFSIGSNDMTQLTLGLDRDSGVV
HHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHH
SALFDERNAAVKALLSMAIRSAKAQGKYVGICGQGPSDHEDFARWLMEQGIDSLSLNPDT
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHH
VAQTWINLAEK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]