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The map label for this gene is pspF [H]

Identifier: 238919727

GI number: 238919727

Start: 1769903

End: 1770925

Strand: Reverse

Name: pspF [H]

Synonym: NT01EI_1831

Alternate gene names: 238919727

Gene position: 1770925-1769903 (Counterclockwise)

Preceding gene: 238919734

Following gene: 238919726

Centisome position: 46.45

GC content: 67.06

Gene sequence:

>1023_bases
ATGGCCGATCGCGATACCCTACTGGGCGAAGACAACGCGTTTCTGGAGGTGCTGGAGCAGACCTCACGCCTGGCTCAATT
GAACAAGCCGCTGCTGATCCTCGGCGAACGCGGCACCGGTAAGGAGCTGATCGCCCAGCGCCTGCACTATCTTTCGCCGC
GCTGGCAGGGGCCGTTTATCTCCCTAAACTGCGCCGCGCTCAGCGAGACCCTGCTGGACTCCGAGCTGTTCGGCCATGAG
GCCGGCGCCTTCACCGGCGCCCAGCGTCGTCATCTGGGGCGCTTTGAGCGCGCCGACGGCGGCACCCTGTTTCTCGATGA
GCTGGCCACCGCGCCGATGCTGATCCAGGAAAAGCTGCTGCGAGTCATCGAGTATGGCCATCTGGAGCGCGTCGGCGGCA
GCCGGTCGCTGCAGGTTGACGTACGCTTGGTCTGTGCCACCAATGCCGATCTGCCAGCGCTGGCCGCCGCGGGGCAGTTT
CGCGCCGATCTGCTGGATCGCCTGGCGTTTGAGGTCATCGCCCTGCCGCCGCTGCGCCGGCGCCGGGCTGACATCCTGCC
GCTGGCGGAGCACTTCGCCATGCAGATGTGCCGGGAGCTGGGGCGGGCGCTGTTCGGTGGCTTCAGCGCCCGCGCGCAGC
GTACGCTGCTCGACTACCGCTGGCCCGGCAACATCCGCGAGCTGAAAAACGTGGTGGAGCGCTCCGTGTTTCGCCACGGC
GACGACGAAGGCGCACTGCAGAACATCGTGCTTAATCCCTTCCCCACTCCCGACGCTGCCGTGGATGATGAGAGGGCCGC
GGCGCTGCCGACGCTGCCGCTGGATCTGCGCGCCTGGCAGAGCGAACAGGAGTGCCGCCTGCTACGCCACGCGCTGCAAC
AGGCGCACTATAACCAGCGGCGCGCCGCCGACCTGCTCGGCATCACCTACCATCAGCTGCGCGGCATTATGAAAAAGCAG
GGGATCGCGGCGATACCGCCGCAGACGGCCGACGATGCCGCGGCGATGACACCGCGCGAATGA

Upstream 100 bases:

>100_bases
ACAATTCAAAGTGTTAATTATATTCCCGCCGGAGGATTGCAGAAACAGGAGTGATGAGTTTTACTAAAAGATAGTAAAAA
TAACCACATTAAGGTGAGAG

Downstream 100 bases:

>100_bases
GGCCGACGACGGGATAAAAAAAAGCCAGCACCCGCGCTGGCATTAAAATGAAACTGTAAGCAATGTGAGCAATGTCGTGC
TCCCCGCAGGCGCCTGTCAG

Product: phage shock protein operon transcriptional activator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MADRDTLLGEDNAFLEVLEQTSRLAQLNKPLLILGERGTGKELIAQRLHYLSPRWQGPFISLNCAALSETLLDSELFGHE
AGAFTGAQRRHLGRFERADGGTLFLDELATAPMLIQEKLLRVIEYGHLERVGGSRSLQVDVRLVCATNADLPALAAAGQF
RADLLDRLAFEVIALPPLRRRRADILPLAEHFAMQMCRELGRALFGGFSARAQRTLLDYRWPGNIRELKNVVERSVFRHG
DDEGALQNIVLNPFPTPDAAVDDERAAALPTLPLDLRAWQSEQECRLLRHALQQAHYNQRRAADLLGITYHQLRGIMKKQ
GIAAIPPQTADDAAAMTPRE

Sequences:

>Translated_340_residues
MADRDTLLGEDNAFLEVLEQTSRLAQLNKPLLILGERGTGKELIAQRLHYLSPRWQGPFISLNCAALSETLLDSELFGHE
AGAFTGAQRRHLGRFERADGGTLFLDELATAPMLIQEKLLRVIEYGHLERVGGSRSLQVDVRLVCATNADLPALAAAGQF
RADLLDRLAFEVIALPPLRRRRADILPLAEHFAMQMCRELGRALFGGFSARAQRTLLDYRWPGNIRELKNVVERSVFRHG
DDEGALQNIVLNPFPTPDAAVDDERAAALPTLPLDLRAWQSEQECRLLRHALQQAHYNQRRAADLLGITYHQLRGIMKKQ
GIAAIPPQTADDAAAMTPRE
>Mature_339_residues
ADRDTLLGEDNAFLEVLEQTSRLAQLNKPLLILGERGTGKELIAQRLHYLSPRWQGPFISLNCAALSETLLDSELFGHEA
GAFTGAQRRHLGRFERADGGTLFLDELATAPMLIQEKLLRVIEYGHLERVGGSRSLQVDVRLVCATNADLPALAAAGQFR
ADLLDRLAFEVIALPPLRRRRADILPLAEHFAMQMCRELGRALFGGFSARAQRTLLDYRWPGNIRELKNVVERSVFRHGD
DEGALQNIVLNPFPTPDAAVDDERAAALPTLPLDLRAWQSEQECRLLRHALQQAHYNQRRAADLLGITYHQLRGIMKKQG
IAAIPPQTADDAAAMTPRE

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=321, Percent_Identity=71.0280373831776, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1788550, Length=319, Percent_Identity=41.692789968652, Blast_Score=230, Evalue=8e-62,
Organism=Escherichia coli, GI87082117, Length=329, Percent_Identity=41.6413373860182, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1789087, Length=321, Percent_Identity=42.0560747663551, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1790437, Length=312, Percent_Identity=41.9871794871795, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI87082152, Length=326, Percent_Identity=39.5705521472393, Blast_Score=210, Evalue=9e-56,
Organism=Escherichia coli, GI1790299, Length=333, Percent_Identity=39.039039039039, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=39.0625, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788905, Length=305, Percent_Identity=39.0163934426229, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786524, Length=318, Percent_Identity=39.3081761006289, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=35.4838709677419, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081858, Length=319, Percent_Identity=32.9153605015674, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=34.6320346320346, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 37916; Mature: 37785

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRDTLLGEDNAFLEVLEQTSRLAQLNKPLLILGERGTGKELIAQRLHYLSPRWQGPFI
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEE
SLNCAALSETLLDSELFGHEAGAFTGAQRRHLGRFERADGGTLFLDELATAPMLIQEKLL
EEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCEEEHHHHCCCHHHHHHHHH
RVIEYGHLERVGGSRSLQVDVRLVCATNADLPALAAAGQFRADLLDRLAFEVIALPPLRR
HHHHHCCHHHCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RRADILPLAEHFAMQMCRELGRALFGGFSARAQRTLLDYRWPGNIRELKNVVERSVFRHG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DDEGALQNIVLNPFPTPDAAVDDERAAALPTLPLDLRAWQSEQECRLLRHALQQAHYNQR
CCCHHHHHHEECCCCCCCCCCCCCHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RAADLLGITYHQLRGIMKKQGIAAIPPQTADDAAAMTPRE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADRDTLLGEDNAFLEVLEQTSRLAQLNKPLLILGERGTGKELIAQRLHYLSPRWQGPFI
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCEE
SLNCAALSETLLDSELFGHEAGAFTGAQRRHLGRFERADGGTLFLDELATAPMLIQEKLL
EEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCCEEEHHHHCCCHHHHHHHHH
RVIEYGHLERVGGSRSLQVDVRLVCATNADLPALAAAGQFRADLLDRLAFEVIALPPLRR
HHHHHCCHHHCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
RRADILPLAEHFAMQMCRELGRALFGGFSARAQRTLLDYRWPGNIRELKNVVERSVFRHG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
DDEGALQNIVLNPFPTPDAAVDDERAAALPTLPLDLRAWQSEQECRLLRHALQQAHYNQR
CCCHHHHHHEECCCCCCCCCCCCCHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RAADLLGITYHQLRGIMKKQGIAAIPPQTADDAAAMTPRE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]