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The map label for this gene is sapA [H]

Identifier: 238919726

GI number: 238919726

Start: 1767831

End: 1769501

Strand: Reverse

Name: sapA [H]

Synonym: NT01EI_1829

Alternate gene names: 238919726

Gene position: 1769501-1767831 (Counterclockwise)

Preceding gene: 238919727

Following gene: 238919725

Centisome position: 46.42

GC content: 61.88

Gene sequence:

>1671_bases
ATGCGCGCTCTGAAAACACTGTTTATTCCGCTGCTACTCCTGTCAGCAGCGGTCACGGCGGCCGGGAAACCGGCGCCGCA
GCCCGCCCCCGCTGTGCCGGATATCCGCCAGAGCGGGTTTATCTATTGCGTCAACGGTATCGTCAATACCTTCAATCCGC
AGATGGCCAGCAGCGGCGTGACGGTGGATACCTTGGCGGCCCAGCTCTACGACCGACTGCTGGATGTCGACCCCTATACC
TATCGCCTGATCCCCGAGCTGGCCCAACGTTGGGAGGTGCTCGACGGCGGCGCGACCTATATTCTGCACCTGCGCCGCGA
TGTTCCGTTCCAGAAAACCGCTTGGTTCACCCCGACCCGCAACATGAACGCCGACGATGTGCTGTTCAGTTTCGAGCGCA
TGTTCGACCCCAAACACCCCTATCACGACGTCAACGGCGGTCACTATCCCTACTTCGACAGTCTGCAGTTCAGCAATGCG
GTACAGAGTATCCGCAGGCTGGACGATAACAGCGTCGAGATTCGCCTGAAGCAGCCTGACGCCTCTTTTCTCTGGCATCT
TGCCACCCACTACGCACCGGTGCTCTCGGCCCAGTACGCCGATGCGCTGGCCAAGCGTGGCCAGCAGGTGCAGATAGACC
GGCTGCCGGTCGGCACCGGCCCCTTTATGCTGGATGAGTATCGCGCCGGGCAGTTTATCCGGCTGACGCGTAACCCGCGG
TACTGGAAAGGGACGCCGCGCATGACACAGGTGGTCATCGACCTGGGCGCCGGCGGCACCGGACGGATCTCTAAGCTGCT
GACCGGCGAGTGCGATGTACTGGCCTATCCCGCGGCCAGCCAGCTGGGCATACTGCGCGACGATCCGCGCCTGCGCCTCA
CCCTGCGTCCGGGGATGAACGTCGCCTATCTGGCGTTTAACACCCGTAAGCCGCCTCTGGACAACGTCGATGTACGCAAC
GCCATCGCCTATGCGATCAATAACCAGCGCCTGATGCAGTCCATTTATTATGGCACGGCGGAGACGGCAGCCTCCCTGCT
GCCACGCGCATCCTGGGCCTACGACAATGACGCGCGCGTTACCGACTACAATCCGGAGAAGGCGCGCGCCATGCTCAAGT
CCGCCGGGATCAGCAATCTACGTCTGCAGCTGTGGGTACCAACCGCCTCCCAGGCCTATAATCCCAGCCCACTGAAAACC
GCCGAGCTGATCCAGGCCGACTTGGCCCAGGTCGGCATCAAGGTCACCATTGTGCCGGTGGAGGGGCGCTTTCAGGAGGC
CCGTCTGAATGATATGAGCCATGATATGACCCTCTCCGGCTGGGCGACCGACAGCAACGATCCGGACAGCTTCTTCCGGC
CGTTGCTCAGCTGCGCCGCCATTCAGTCCCAGACCAATCTGGCCCACTGGTGTGATCCGGCCTTCGATACCCTGCTGCAC
CAGGCACTGCAGTCCCAGCAGCTCTCCCAGCGGATTGACGACTATCAGGCGGCGCAAAAAATCCTGGAGCAGCAGCTGCC
GATCCTGCCGCTGGCCTCTTCGCTTCGCCTGCAGGCCTATCGCCATGACATCAAAGGACTGGTGCTCAGTCCATTCGGCA
ACGCCTCGTTCGCAGGGGTATACCGTGATATACCGAGCCCGACGCCCGCGCCGGACAAGGAGAAACCATGA

Upstream 100 bases:

>100_bases
CTGCCGCTGCTCCGTCGTGTCATGGATCCGACGCAGGCAGCGTGCCGGGCCTCTCTGCCTGCGCCGCCGGCAGCACAGCC
AACAACCTGTTGAAGCCTCT

Downstream 100 bases:

>100_bases
TTATTTTTACCCTGCGGCGTCTGCTGCTGCTGCTGGTCACACTGTTTTTCCTGACCCTCGTCAGCTTCAGCCTGCTCTAC
TTTACGCCCCATGCCCCGCT

Product: peptide transport periplasmic protein SapA

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MRALKTLFIPLLLLSAAVTAAGKPAPQPAPAVPDIRQSGFIYCVNGIVNTFNPQMASSGVTVDTLAAQLYDRLLDVDPYT
YRLIPELAQRWEVLDGGATYILHLRRDVPFQKTAWFTPTRNMNADDVLFSFERMFDPKHPYHDVNGGHYPYFDSLQFSNA
VQSIRRLDDNSVEIRLKQPDASFLWHLATHYAPVLSAQYADALAKRGQQVQIDRLPVGTGPFMLDEYRAGQFIRLTRNPR
YWKGTPRMTQVVIDLGAGGTGRISKLLTGECDVLAYPAASQLGILRDDPRLRLTLRPGMNVAYLAFNTRKPPLDNVDVRN
AIAYAINNQRLMQSIYYGTAETAASLLPRASWAYDNDARVTDYNPEKARAMLKSAGISNLRLQLWVPTASQAYNPSPLKT
AELIQADLAQVGIKVTIVPVEGRFQEARLNDMSHDMTLSGWATDSNDPDSFFRPLLSCAAIQSQTNLAHWCDPAFDTLLH
QALQSQQLSQRIDDYQAAQKILEQQLPILPLASSLRLQAYRHDIKGLVLSPFGNASFAGVYRDIPSPTPAPDKEKP

Sequences:

>Translated_556_residues
MRALKTLFIPLLLLSAAVTAAGKPAPQPAPAVPDIRQSGFIYCVNGIVNTFNPQMASSGVTVDTLAAQLYDRLLDVDPYT
YRLIPELAQRWEVLDGGATYILHLRRDVPFQKTAWFTPTRNMNADDVLFSFERMFDPKHPYHDVNGGHYPYFDSLQFSNA
VQSIRRLDDNSVEIRLKQPDASFLWHLATHYAPVLSAQYADALAKRGQQVQIDRLPVGTGPFMLDEYRAGQFIRLTRNPR
YWKGTPRMTQVVIDLGAGGTGRISKLLTGECDVLAYPAASQLGILRDDPRLRLTLRPGMNVAYLAFNTRKPPLDNVDVRN
AIAYAINNQRLMQSIYYGTAETAASLLPRASWAYDNDARVTDYNPEKARAMLKSAGISNLRLQLWVPTASQAYNPSPLKT
AELIQADLAQVGIKVTIVPVEGRFQEARLNDMSHDMTLSGWATDSNDPDSFFRPLLSCAAIQSQTNLAHWCDPAFDTLLH
QALQSQQLSQRIDDYQAAQKILEQQLPILPLASSLRLQAYRHDIKGLVLSPFGNASFAGVYRDIPSPTPAPDKEKP
>Mature_556_residues
MRALKTLFIPLLLLSAAVTAAGKPAPQPAPAVPDIRQSGFIYCVNGIVNTFNPQMASSGVTVDTLAAQLYDRLLDVDPYT
YRLIPELAQRWEVLDGGATYILHLRRDVPFQKTAWFTPTRNMNADDVLFSFERMFDPKHPYHDVNGGHYPYFDSLQFSNA
VQSIRRLDDNSVEIRLKQPDASFLWHLATHYAPVLSAQYADALAKRGQQVQIDRLPVGTGPFMLDEYRAGQFIRLTRNPR
YWKGTPRMTQVVIDLGAGGTGRISKLLTGECDVLAYPAASQLGILRDDPRLRLTLRPGMNVAYLAFNTRKPPLDNVDVRN
AIAYAINNQRLMQSIYYGTAETAASLLPRASWAYDNDARVTDYNPEKARAMLKSAGISNLRLQLWVPTASQAYNPSPLKT
AELIQADLAQVGIKVTIVPVEGRFQEARLNDMSHDMTLSGWATDSNDPDSFFRPLLSCAAIQSQTNLAHWCDPAFDTLLH
QALQSQQLSQRIDDYQAAQKILEQQLPILPLASSLRLQAYRHDIKGLVLSPFGNASFAGVYRDIPSPTPAPDKEKP

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=540, Percent_Identity=74.2592592592593, Blast_Score=826, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=507, Percent_Identity=37.2781065088757, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1787052, Length=506, Percent_Identity=26.8774703557312, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787762, Length=489, Percent_Identity=24.9488752556237, Blast_Score=138, Evalue=8e-34,
Organism=Escherichia coli, GI1789397, Length=541, Percent_Identity=27.7264325323475, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789887, Length=488, Percent_Identity=25.4098360655738, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787495, Length=478, Percent_Identity=25.1046025104602, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081878, Length=463, Percent_Identity=24.8380129589633, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62014; Mature: 62014

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00027 HOMEOBOX_1 ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALKTLFIPLLLLSAAVTAAGKPAPQPAPAVPDIRQSGFIYCVNGIVNTFNPQMASSGV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCEEEEECCHHHCCCCHHHCCCC
TVDTLAAQLYDRLLDVDPYTYRLIPELAQRWEVLDGGATYILHLRRDVPFQKTAWFTPTR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCEEECCCC
NMNADDVLFSFERMFDPKHPYHDVNGGHYPYFDSLQFSNAVQSIRRLDDNSVEIRLKQPD
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCC
ASFLWHLATHYAPVLSAQYADALAKRGQQVQIDRLPVGTGPFMLDEYRAGQFIRLTRNPR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEHHCCCCCEEEEECCCC
YWKGTPRMTQVVIDLGAGGTGRISKLLTGECDVLAYPAASQLGILRDDPRLRLTLRPGMN
CCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCEEECCCHHHCCEEECCCCEEEEECCCCC
VAYLAFNTRKPPLDNVDVRNAIAYAINNQRLMQSIYYGTAETAASLLPRASWAYDNDARV
EEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEE
TDYNPEKARAMLKSAGISNLRLQLWVPTASQAYNPSPLKTAELIQADLAQVGIKVTIVPV
CCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEC
EGRFQEARLNDMSHDMTLSGWATDSNDPDSFFRPLLSCAAIQSQTNLAHWCDPAFDTLLH
CCCCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHH
QALQSQQLSQRIDDYQAAQKILEQQLPILPLASSLRLQAYRHDIKGLVLSPFGNASFAGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHH
YRDIPSPTPAPDKEKP
HHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRALKTLFIPLLLLSAAVTAAGKPAPQPAPAVPDIRQSGFIYCVNGIVNTFNPQMASSGV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCEEEEECCHHHCCCCHHHCCCC
TVDTLAAQLYDRLLDVDPYTYRLIPELAQRWEVLDGGATYILHLRRDVPFQKTAWFTPTR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCEEECCCC
NMNADDVLFSFERMFDPKHPYHDVNGGHYPYFDSLQFSNAVQSIRRLDDNSVEIRLKQPD
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCC
ASFLWHLATHYAPVLSAQYADALAKRGQQVQIDRLPVGTGPFMLDEYRAGQFIRLTRNPR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEHHCCCCCEEEEECCCC
YWKGTPRMTQVVIDLGAGGTGRISKLLTGECDVLAYPAASQLGILRDDPRLRLTLRPGMN
CCCCCCCEEEEEEEECCCCCHHHHHHHCCCCCEEECCCHHHCCEEECCCCEEEEECCCCC
VAYLAFNTRKPPLDNVDVRNAIAYAINNQRLMQSIYYGTAETAASLLPRASWAYDNDARV
EEEEEEECCCCCCCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCEE
TDYNPEKARAMLKSAGISNLRLQLWVPTASQAYNPSPLKTAELIQADLAQVGIKVTIVPV
CCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEC
EGRFQEARLNDMSHDMTLSGWATDSNDPDSFFRPLLSCAAIQSQTNLAHWCDPAFDTLLH
CCCCHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHH
QALQSQQLSQRIDDYQAAQKILEQQLPILPLASSLRLQAYRHDIKGLVLSPFGNASFAGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCHHHH
YRDIPSPTPAPDKEKP
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]