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The map label for this gene is pepT

Identifier: 238919716

GI number: 238919716

Start: 1756014

End: 1757252

Strand: Direct

Name: pepT

Synonym: NT01EI_1819

Alternate gene names: 238919716

Gene position: 1756014-1757252 (Clockwise)

Preceding gene: 238919710

Following gene: 238919720

Centisome position: 46.06

GC content: 60.86

Gene sequence:

>1239_bases
ATGAATAACTTACTTGATCGTTTTCTTAACTATGTTTCGTTTGACACACAGTCCAAGCCCGGAGTACGTCAGGTTCCGAG
CACCGAGGGACAGTTCAGGCTGGCCCGCGCACTGCAAAGCGAGCTGCTGGCGCTGGGGCTGGAGCAGGTCACGCTCAGCG
AACACGGCTGCGTGATGGCGACGCTGCCCGCCAACGTGGCCTGGCCGGTGCCAACCATCGGTTTTATCGCCCATATGGAT
ACGGCGCCGGACGCCAGTGGTAAAAACGTGAATCCGCAGATCGTCGAAAATTACCGCGGTGGCGATATCGCCCTGGGGAT
CGGCGATGAAATTCTCTCTCCGGTGATGTTTCCGGTGCTGCACCAGCTGCTGGGGCAGACCCTGATCACCACCGACGGCA
AAACCCTGCTGGGGGCCGACGATAAGTCGGGGATCGCCGAAATTATGACGGCGATGGTGCGTCTCAAGCAGGGAAATACC
CCCCACGGTGAGATCCGGGTAGCCTTTACCCCGGATGAAGAGGTGGGTAAGGGCGCCCAGCATTTCGACGTCGCGGCCTT
TGGCGCGGAGTGGGCCTACACCGTGGACGGTGGTGGCGTGGGCGAGCTGGAGTGTGAAAACTTTAATGCGGCCTCCGTCA
ACATCAAGATCATCGGCAATAACGTACATCCGGGCAGCGCCAAGGGGGTGATGGTTAACGCCCTGGGGTTGGCAAACCGT
ATTCATGCGCTGCTGCCGGCGGCGGAGGTGCCGGAGCAGACCGACGGCTATGAGGGGTTCTACCACCTGGTGTCGATGAA
GGGCAGCGTGGAGAAGGCCGAGATGCACTACATCGTGCGCGACTTCAGCCGCGAGGGGTTTGAGGCGCGCAAGAAGCACA
TGATGGCGATTGCCAAGCAGGTGGGCCAGGGATTGCACCCGGACTGCTACATCGAGGTGACGCTGGACGACAGTTACTAC
AATATGCGTGACGAGGTGGCGAAGCACCCTCATATCGTCGCCCTGGCGCGTCAGGCGATGTGCGATCTGGCGATCGAGCC
CATCGAGCGGCCGATCCGCGGCGGCACCGATGGGGCGCAGCTGTCATTCCACGGCCTGCCGTGCCCGAACCTGTTTACCG
GTGGCTATAACTTCCACGGCAAGCATGAGTTTATTACGCTGGAGGGGATGGAGAAGGCGGTATCGGTGATCATGCGTATC
GCTGAGCTGACCGCCGGGCGCGCACGCGTCGCCGGATAA

Upstream 100 bases:

>100_bases
TGTCAGGAGAAAAGGCAAACCCCGGTAAAAATGACCTGACGCAATATTTCGCCGGAGGTGGCTCCGCATAATGGGGCAAC
AAGAAGGGAGAAAATACCAA

Downstream 100 bases:

>100_bases
TCTGGACCGCTGTACGGCGGCCGGGGATGGCCGTCGTATCCGGGGTGTCGCGCGATGGGCGGGGCACCCCGTGTTTTGCG
CCGGATTCAGCAGGTGCCGT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MNNLLDRFLNYVSFDTQSKPGVRQVPSTEGQFRLARALQSELLALGLEQVTLSEHGCVMATLPANVAWPVPTIGFIAHMD
TAPDASGKNVNPQIVENYRGGDIALGIGDEILSPVMFPVLHQLLGQTLITTDGKTLLGADDKSGIAEIMTAMVRLKQGNT
PHGEIRVAFTPDEEVGKGAQHFDVAAFGAEWAYTVDGGGVGELECENFNAASVNIKIIGNNVHPGSAKGVMVNALGLANR
IHALLPAAEVPEQTDGYEGFYHLVSMKGSVEKAEMHYIVRDFSREGFEARKKHMMAIAKQVGQGLHPDCYIEVTLDDSYY
NMRDEVAKHPHIVALARQAMCDLAIEPIERPIRGGTDGAQLSFHGLPCPNLFTGGYNFHGKHEFITLEGMEKAVSVIMRI
AELTAGRARVAG

Sequences:

>Translated_412_residues
MNNLLDRFLNYVSFDTQSKPGVRQVPSTEGQFRLARALQSELLALGLEQVTLSEHGCVMATLPANVAWPVPTIGFIAHMD
TAPDASGKNVNPQIVENYRGGDIALGIGDEILSPVMFPVLHQLLGQTLITTDGKTLLGADDKSGIAEIMTAMVRLKQGNT
PHGEIRVAFTPDEEVGKGAQHFDVAAFGAEWAYTVDGGGVGELECENFNAASVNIKIIGNNVHPGSAKGVMVNALGLANR
IHALLPAAEVPEQTDGYEGFYHLVSMKGSVEKAEMHYIVRDFSREGFEARKKHMMAIAKQVGQGLHPDCYIEVTLDDSYY
NMRDEVAKHPHIVALARQAMCDLAIEPIERPIRGGTDGAQLSFHGLPCPNLFTGGYNFHGKHEFITLEGMEKAVSVIMRI
AELTAGRARVAG
>Mature_412_residues
MNNLLDRFLNYVSFDTQSKPGVRQVPSTEGQFRLARALQSELLALGLEQVTLSEHGCVMATLPANVAWPVPTIGFIAHMD
TAPDASGKNVNPQIVENYRGGDIALGIGDEILSPVMFPVLHQLLGQTLITTDGKTLLGADDKSGIAEIMTAMVRLKQGNT
PHGEIRVAFTPDEEVGKGAQHFDVAAFGAEWAYTVDGGGVGELECENFNAASVNIKIIGNNVHPGSAKGVMVNALGLANR
IHALLPAAEVPEQTDGYEGFYHLVSMKGSVEKAEMHYIVRDFSREGFEARKKHMMAIAKQVGQGLHPDCYIEVTLDDSYY
NMRDEVAKHPHIVALARQAMCDLAIEPIERPIRGGTDGAQLSFHGLPCPNLFTGGYNFHGKHEFITLEGMEKAVSVIMRI
AELTAGRARVAG

Specific function: Cleaves the N-terminal amino acid of tripeptides

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=407, Percent_Identity=77.3955773955774, Blast_Score=664, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPT_EDWI9 (C5BFN3)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933231.1
- GeneID:   7962140
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1819
- OMA:   HMDTAPD
- ProtClustDB:   PRK05469
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.4.11.4

Molecular weight: Translated: 44584; Mature: 44584

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 80-80 ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLLDRFLNYVSFDTQSKPGVRQVPSTEGQFRLARALQSELLALGLEQVTLSEHGCVMA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
TLPANVAWPVPTIGFIAHMDTAPDASGKNVNPQIVENYRGGDIALGIGDEILSPVMFPVL
ECCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHH
HQLLGQTLITTDGKTLLGADDKSGIAEIMTAMVRLKQGNTPHGEIRVAFTPDEEVGKGAQ
HHHHCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCC
HFDVAAFGAEWAYTVDGGGVGELECENFNAASVNIKIIGNNVHPGSAKGVMVNALGLANR
CEEEEEECCCEEEEECCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHHH
IHALLPAAEVPEQTDGYEGFYHLVSMKGSVEKAEMHYIVRDFSREGFEARKKHMMAIAKQ
HHHHCCHHHCCCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VGQGLHPDCYIEVTLDDSYYNMRDEVAKHPHIVALARQAMCDLAIEPIERPIRGGTDGAQ
HCCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LSFHGLPCPNLFTGGYNFHGKHEFITLEGMEKAVSVIMRIAELTAGRARVAG
EEECCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNNLLDRFLNYVSFDTQSKPGVRQVPSTEGQFRLARALQSELLALGLEQVTLSEHGCVMA
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEE
TLPANVAWPVPTIGFIAHMDTAPDASGKNVNPQIVENYRGGDIALGIGDEILSPVMFPVL
ECCCCCCCCCCHHHHHEECCCCCCCCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHH
HQLLGQTLITTDGKTLLGADDKSGIAEIMTAMVRLKQGNTPHGEIRVAFTPDEEVGKGAQ
HHHHCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHCCCCC
HFDVAAFGAEWAYTVDGGGVGELECENFNAASVNIKIIGNNVHPGSAKGVMVNALGLANR
CEEEEEECCCEEEEECCCCCCCEEECCCCEEEEEEEEEECCCCCCCCCCEEEEHHHHHHH
IHALLPAAEVPEQTDGYEGFYHLVSMKGSVEKAEMHYIVRDFSREGFEARKKHMMAIAKQ
HHHHCCHHHCCCCCCCCCHHEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VGQGLHPDCYIEVTLDDSYYNMRDEVAKHPHIVALARQAMCDLAIEPIERPIRGGTDGAQ
HCCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LSFHGLPCPNLFTGGYNFHGKHEFITLEGMEKAVSVIMRIAELTAGRARVAG
EEECCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA