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The map label for this gene is melA [H]

Identifier: 238919676

GI number: 238919676

Start: 1721887

End: 1723239

Strand: Direct

Name: melA [H]

Synonym: NT01EI_1777

Alternate gene names: 238919676

Gene position: 1721887-1723239 (Clockwise)

Preceding gene: 238919674

Following gene: 238919677

Centisome position: 45.17

GC content: 60.61

Gene sequence:

>1353_bases
ATGAACGCACCTAAAATTGCCTTTATTGGCGCAGGTTCAACCATTTTTGTGAAGAATATTCTGGGGGATGTATTCCAGCG
CCCCGCGCTGCGGGGAGCCGATATCGCGTTGATGGATATCGATGCGCAGCGCCTGGCCGAGTCGCGGATCGTCGTTGGCC
GGCTGATGGCGGCGGCCGGAGCTGACGGACACATTCAGTGCCATACGACGTTGCGTCCGGCGCTGCAGGGGGCCGATTTT
GTGGTGGTGGCGTTTCAGATCGGTGGCTATACGCCCTGCACGGTCACCGATTTTGAGGTCTGTGCACGCCATGGCCTGCG
GCAGACCATCGGCGACACCCTGGGGCCGGCCGGCATTATGCGTGCGCTGCGTACCTTTCCACACCTGTGGCAGATAGGCG
AGATGATGATGGCGGAGTGTCCTCAGGCGACGCTGCTAAACTATGTCAATCCGATGGCGATGAATAGCTGGGCTATGTAT
GCGCGCTATCCGCAGCTGCGGCAGGTCGGTCTATGCCACTCGGTACAGGGCACGGCGGAGGAGCTGGCGCGGGATCTCGA
TATCGATCCGGCGCAGCTACGCTACCGCTGCGCCGGGATTAACCATATGGCCTTCTATCTTTCGCTGGAGCAGCGCGGCG
GGGACGGTCAGTATCGCGATCTCTACCCGCAGCTGCTGCAGGCCTATCGTCAGGGGCGGGCGCCGAAGCCGAACATACAT
GGCAACCTGCGCTGCGAGAATATCGTACGCTATGAGCTGCTGCAAAAACTGGGTTACTTTGTCACGGAGTCCTCAGAGCA
TATGGCCGAATATACGCCGTGGTTTATCAAGCCTGGGCGCGATGATCTGATCGCGCGTTACCGCATCCCGCTGGATGAGT
ATCCCAAACGCTGCGTGGAGCAGATGGCGAGCTGGCAGCGCGAGCTGGCAGAGTATCAGGCGGCGCCGCATATCGAGATT
ACGCCATCGCGGGAGTACGCCAGCCGGATCATGAATGCGCTGTGGAGTGGGGAGCCAGAGGTGATCTATGGCAACGTGCG
TAACGCGGGCCTGATCGACAACCTGCCACAGGGTTGCTGTGTGGAAGTTCCCTGTCTGGTCGATGCCAATGGGATCCAGC
CGACGCGGATCGGGTCGCTGCCCTCGCACCTGGCGGCGCTGATGCAGACCAATGTCAATGTGCAGACGCTGGTAACGGAG
GCAGCGCTGACGGAAAACCGCGAGCGGATATACCATGCCGCACTGCTCGATCCGCATACGGCTGCGACGCTGAGTATCGA
TGAGATCTATGCCCTGATCGACGATCTCATTGCGGCGCACGGTGACTGGCTGCCTGACTGGGTGCGCGGCTGA

Upstream 100 bases:

>100_bases
AAATCCGAGGAAATGGGACTGCTTTGGTAAGAAGGCGGACGCGCGCGTCCGCTGTGCGTGTCACGATGCCTCCAGCGATG
GCTTTTATGGGAGAGAGACG

Downstream 100 bases:

>100_bases
GCCGGCAGCGTGTGGTGCTTTCCGGCGTTGGCTGGCGCAGCGGAAAATCAGACGATCCCTTAATTCCCTGCGCCGGGCTA
TTAGGTACTATGAACTTCCG

Product: alpha-galactosidase

Products: NA

Alternate protein names: Melibiase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNAPKIAFIGAGSTIFVKNILGDVFQRPALRGADIALMDIDAQRLAESRIVVGRLMAAAGADGHIQCHTTLRPALQGADF
VVVAFQIGGYTPCTVTDFEVCARHGLRQTIGDTLGPAGIMRALRTFPHLWQIGEMMMAECPQATLLNYVNPMAMNSWAMY
ARYPQLRQVGLCHSVQGTAEELARDLDIDPAQLRYRCAGINHMAFYLSLEQRGGDGQYRDLYPQLLQAYRQGRAPKPNIH
GNLRCENIVRYELLQKLGYFVTESSEHMAEYTPWFIKPGRDDLIARYRIPLDEYPKRCVEQMASWQRELAEYQAAPHIEI
TPSREYASRIMNALWSGEPEVIYGNVRNAGLIDNLPQGCCVEVPCLVDANGIQPTRIGSLPSHLAALMQTNVNVQTLVTE
AALTENRERIYHAALLDPHTAATLSIDEIYALIDDLIAAHGDWLPDWVRG

Sequences:

>Translated_450_residues
MNAPKIAFIGAGSTIFVKNILGDVFQRPALRGADIALMDIDAQRLAESRIVVGRLMAAAGADGHIQCHTTLRPALQGADF
VVVAFQIGGYTPCTVTDFEVCARHGLRQTIGDTLGPAGIMRALRTFPHLWQIGEMMMAECPQATLLNYVNPMAMNSWAMY
ARYPQLRQVGLCHSVQGTAEELARDLDIDPAQLRYRCAGINHMAFYLSLEQRGGDGQYRDLYPQLLQAYRQGRAPKPNIH
GNLRCENIVRYELLQKLGYFVTESSEHMAEYTPWFIKPGRDDLIARYRIPLDEYPKRCVEQMASWQRELAEYQAAPHIEI
TPSREYASRIMNALWSGEPEVIYGNVRNAGLIDNLPQGCCVEVPCLVDANGIQPTRIGSLPSHLAALMQTNVNVQTLVTE
AALTENRERIYHAALLDPHTAATLSIDEIYALIDDLIAAHGDWLPDWVRG
>Mature_450_residues
MNAPKIAFIGAGSTIFVKNILGDVFQRPALRGADIALMDIDAQRLAESRIVVGRLMAAAGADGHIQCHTTLRPALQGADF
VVVAFQIGGYTPCTVTDFEVCARHGLRQTIGDTLGPAGIMRALRTFPHLWQIGEMMMAECPQATLLNYVNPMAMNSWAMY
ARYPQLRQVGLCHSVQGTAEELARDLDIDPAQLRYRCAGINHMAFYLSLEQRGGDGQYRDLYPQLLQAYRQGRAPKPNIH
GNLRCENIVRYELLQKLGYFVTESSEHMAEYTPWFIKPGRDDLIARYRIPLDEYPKRCVEQMASWQRELAEYQAAPHIEI
TPSREYASRIMNALWSGEPEVIYGNVRNAGLIDNLPQGCCVEVPCLVDANGIQPTRIGSLPSHLAALMQTNVNVQTLVTE
AALTENRERIYHAALLDPHTAATLSIDEIYALIDDLIAAHGDWLPDWVRG

Specific function: Unknown

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=448, Percent_Identity=78.125, Blast_Score=757, Evalue=0.0,
Organism=Escherichia coli, GI1788029, Length=463, Percent_Identity=23.110151187905, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 50190; Mature: 50190

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPKIAFIGAGSTIFVKNILGDVFQRPALRGADIALMDIDAQRLAESRIVVGRLMAAAG
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
ADGHIQCHTTLRPALQGADFVVVAFQIGGYTPCTVTDFEVCARHGLRQTIGDTLGPAGIM
CCCCEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHCCCHHHHH
RALRTFPHLWQIGEMMMAECPQATLLNYVNPMAMNSWAMYARYPQLRQVGLCHSVQGTAE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCHHHHHCCHHHHHHHHHHCCCCHHH
ELARDLDIDPAQLRYRCAGINHMAFYLSLEQRGGDGQYRDLYPQLLQAYRQGRAPKPNIH
HHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GNLRCENIVRYELLQKLGYFVTESSEHMAEYTPWFIKPGRDDLIARYRIPLDEYPKRCVE
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCHHHHEEEECCHHHHHHHHHH
QMASWQRELAEYQAAPHIEITPSREYASRIMNALWSGEPEVIYGNVRNAGLIDNLPQGCC
HHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCE
VEVPCLVDANGIQPTRIGSLPSHLAALMQTNVNVQTLVTEAALTENRERIYHAALLDPHT
EECCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCC
AATLSIDEIYALIDDLIAAHGDWLPDWVRG
CCEEEHHHHHHHHHHHHHHCCCCCCHHHCC
>Mature Secondary Structure
MNAPKIAFIGAGSTIFVKNILGDVFQRPALRGADIALMDIDAQRLAESRIVVGRLMAAAG
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
ADGHIQCHTTLRPALQGADFVVVAFQIGGYTPCTVTDFEVCARHGLRQTIGDTLGPAGIM
CCCCEEEECCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHHHCCHHHHHHHCCCHHHHH
RALRTFPHLWQIGEMMMAECPQATLLNYVNPMAMNSWAMYARYPQLRQVGLCHSVQGTAE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCHHHHHCCHHHHHHHHHHCCCCHHH
ELARDLDIDPAQLRYRCAGINHMAFYLSLEQRGGDGQYRDLYPQLLQAYRQGRAPKPNIH
HHHHHCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
GNLRCENIVRYELLQKLGYFVTESSEHMAEYTPWFIKPGRDDLIARYRIPLDEYPKRCVE
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCCHHHHEEEECCHHHHHHHHHH
QMASWQRELAEYQAAPHIEITPSREYASRIMNALWSGEPEVIYGNVRNAGLIDNLPQGCC
HHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCE
VEVPCLVDANGIQPTRIGSLPSHLAALMQTNVNVQTLVTEAALTENRERIYHAALLDPHT
EECCEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCC
AATLSIDEIYALIDDLIAAHGDWLPDWVRG
CCEEEHHHHHHHHHHHHHHCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3031590; 7610040; 9278503; 6329200; 2831880 [H]